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Tips for good
hygienic practice
•	 Keep moisture levels to a minimum
•	 Keep food processing plant clean
•	 Follow recognised cleaning schedules
•	 Keep perishable products within the
safe temperature zone
•	 Practice effective personal hygiene
•	 Don’t give micro-organisms time
to multiply
•	 Practice effective pest and foreign
body control
•	 Store raw and cooked foods
separately
0 20 min 40 min 1 h 2 h 3 h 4 h 5 h 6 h 7 hTime
Bacteria
Time effect
1 2 4 8 64 512 4,096 32,768 262,144 2,097,152 Typical bacteria multiplication rate at optimum temperature.
Temperature effect
91
82
77
71
54
49
43
38
32
21
7
0
-18
-23
Spores can still
survive at this
temperature
Bacterial
multiplication
is reduced
considerably at
these and higher
temperatures
Food should not
be stored within
this temperature
range for extended
periods of time
Bacteria growth
is slowed
considerably,
which makes
these suitable
refrigeration
temperatures.
Psychrophilic
bacteria may
predominate
Usual
pasteruisation
temperature
Majority of
vegetative
bacteria
destroyed
Optimum
growth
temperature
for pathogenic
bacteria
is 37°C –
human body
temperature
Recommended
temperatures
for storage of
frozen foods
ºC
66
60
Micro-organisms and food
www.diversey.com
© 2018 Diversey, Inc. All Rights Reserved. 66958 en 03/18
CHARACTERISTICS OF THE MAJOR PROBLEM MICRO-ORGANISMS IN THE FOOD AND BEVERAGE INDUSTRIES
PATHOGENICMICRO-ORGANISMS
BACTERIA GROUP DESCRIPTION ORIGIN
CONDITIONS FOR
GROWTH
FOODS
AFFECTED
SOURCE OF
CONTAMINATION
PRINCIPAL SYMPTOMS
TIME OF
INCUBATION
DURATION
OF DISEASE
TEMPERATURE AT
WHICH ORGANISM
IS DESTROYED
STAPHYLOCOCCUS
AUREUS
Gram positive coccus
Hands, hair,
nose, animals,
septic spots, boils
Aerobic and
faculatively anaerobic
Cooked meats, poultry,
egg products, canned
products, milk, cream
Handling of food
and equipment
Nausea, vomiting 2-6 hours 24-48 hours
70°C for
2 minutes toxins
are thermostable
CLOSTRIDIUM
PERFRINGENS
Gram positive, spore
forming bacillus
Faeces, raw meat,
poultry, soil
Anaerobic and
microerophilic
Cooked meats,
poultry, stews, gravies,
canned meats
handling of food and
equipment, soil
Abdominal cramp, diarrhoea 8-24 hours 24-48 hours
Spores are resistant
to boiling
SALMONELLA SPECIES Gram negative rod
Human and
animal intestines,
water bourne
Aerobic and
faculatively anaerobic
Any food of nimal
origin, seafood, egg,
chocolate
Live and slaughtered
animals, handling of
equipment
Acute gastoenteritis,
fever, diarrhoea, vomiting,
septicaemia
12-72 hours 2-5 days 70°C for 2 minutes
ESCHERICHIA COLI Gram negative rod
Human and
animal intestines
Faculatively anaerobic
Meats and derivatives,
cheese, milk products
Handling of food and
equipment
Diarrhoea, dysentry 6-36 hours 1-7 days 70°C for 2 minutes
VEROCYTOTOXIN
(VTEC) PRODUCING
ESCHERICHIA COLI 0157
Gram negative rod
Faeces, raw meat,
raw milk
Aerobic and
faculatively anaerobic
Meats, minced
meats and derivatives,
yogurt, raw milk
Slaughtered animals,
handling of equipment
Severe abdominal pain,
watery diarrhoea with blood,
vomiting, little or no fever, may
cause HUS leading to kidney
dailure and anaemia
3-9 days
6-8 days
Mortality rate
3-5% in cases
with HUS
70°C for 2 minutes
CLOSTRIDIUM
BOTULINUM
Gram positive, spore
forming bacillus
Soil, water Anaerobic
Canned or preserved
foods
External contamination
of equipment
Nausea, vomiting, paralysis 10-48 hours
Approx 10
days – however
mortality rate
is high
Spores are resistant
to boiling
CAMPYLOBACTER
SPECIES
Gram negative bacillus
Faeces, live
animals, water
Faculatively anaerobic
and microaerophilic
Poultry, unpasteurised
milk
Slaughtered animals
Fever, diarrhoea,
abdominal cramp
2-11 days 3-14 days 70°C for 2 minutes
LISTERIA SPECIES Gram positive bacillus Ubiquitous
Aerobic and
faculatively anaerobic
Fish, chicken,cheeses,
raw foods
General environment
Heavy cold, diarrhoea,
septacaemia
1-3 days 3-21 days 70°C for 2 minutes
ROTAVIRUSES
RNA virus
double strand
Faeces,
polluted water
Cell culture in
specific host cells
No specific food
Handling of food,
polluted water
Vomiting, diarrhoea, upper
respiratory tract infections,
may also cause fever
1-7 days
(2-4 days
typical)
2-3 days 70°C for 2 minutes
NORO VIRUS
Believed to be RNA
virus single strand
Faeces,
polluted water
Cell culture in specific
host cells
Shellfish
Handling of food,
polluted water
Nausea, vomiting,
abdominal pain, malaise,
low grade fever
24-72 hours 24-48 hours 80°C for 2 minutes
HEPATITIS A
RNA virus
single strand
Faeces,
polluted water
Cell culture in specific
host cells
Contaminated water,
raw vegetables, shellfish
Handling of food
(faecal-oral route)
polluted water
Fever, malaise,
abdominal pain, jaundice,
liver may be affected
15-50 days
1 week to
several months
80°C for 2 minutes
BACILLUS CEREUS
Gram positive bacillus
sporeforming
Soil, dust, water,
intestines
Aerobic and
faculatively anaerobic
Vegetables, meats,
cereals, milk
General environmental
Gastroenteritis,
vomiting, diarhoea
1-6 hours 6-24 Hours 90°C for 2 minutes
SPOILAGEMICRO-ORGANISMS
LACTOBACILLUS
SPECIES
Gram positive bacillus
Ubiquitous, mouth,
gastrointestinal tract,
widely found in nature
Aerobic some
anaerobic
Dairy, grain, meat, fish
and derived products,
water sewage, beer,
wine, fruit
Plant material, part of
normal gut flora
Little clinical significance,
pathogenicity is rare
Spores are resistant
to boiling
ZYGOSACCHAROMYCES
BAILII
Yeast
Environment, normal
flora of foods
Aerobic and
faculatively anaerobic
High salt, low
preservative, low water
content squashes and
mayonnaise
Inadequately cleaned
food processing plant,
air bourne dust
Little clinical significance,
pathogenicity is rare. Causes
post packing fermentaion
of food products
70°C for 2 minutes
Some species
can survive
pasteurisation
PSEUDOMONAS SPECIES Gram negative rod Water, soil Aerobic Meat, poultry, seafood Water
Infections where moisture
accumulates (e.g. wounds,
burns, urinary tract)
70°C for 2 minutes
RHODOTORULA SPECIES Yeast Air, dust
Aerobic and
faculatively anaerobic
Fresh and refrigerated
meat, poultry, fish,
seafood
Air bourne dust
Little clinical significance,
pathogenicity is very rare.
Produces pink pigments on
meat, fish and poultry
70°C for 2 minutes
SACCHAROMYCES
SPECIES
Yeast
Stored grains, air, dust,
fruit and vegetables
Aerobic and
faculatively anaerobic
Beer, bakery goods Air bourne dust
Little clinical significance,
pathogenicity is very rare.
Produces off flavours in
fermented products
70°C for 2 minutes

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Microorganisms and food_chart

  • 1. Tips for good hygienic practice • Keep moisture levels to a minimum • Keep food processing plant clean • Follow recognised cleaning schedules • Keep perishable products within the safe temperature zone • Practice effective personal hygiene • Don’t give micro-organisms time to multiply • Practice effective pest and foreign body control • Store raw and cooked foods separately 0 20 min 40 min 1 h 2 h 3 h 4 h 5 h 6 h 7 hTime Bacteria Time effect 1 2 4 8 64 512 4,096 32,768 262,144 2,097,152 Typical bacteria multiplication rate at optimum temperature. Temperature effect 91 82 77 71 54 49 43 38 32 21 7 0 -18 -23 Spores can still survive at this temperature Bacterial multiplication is reduced considerably at these and higher temperatures Food should not be stored within this temperature range for extended periods of time Bacteria growth is slowed considerably, which makes these suitable refrigeration temperatures. Psychrophilic bacteria may predominate Usual pasteruisation temperature Majority of vegetative bacteria destroyed Optimum growth temperature for pathogenic bacteria is 37°C – human body temperature Recommended temperatures for storage of frozen foods ºC 66 60 Micro-organisms and food www.diversey.com © 2018 Diversey, Inc. All Rights Reserved. 66958 en 03/18 CHARACTERISTICS OF THE MAJOR PROBLEM MICRO-ORGANISMS IN THE FOOD AND BEVERAGE INDUSTRIES PATHOGENICMICRO-ORGANISMS BACTERIA GROUP DESCRIPTION ORIGIN CONDITIONS FOR GROWTH FOODS AFFECTED SOURCE OF CONTAMINATION PRINCIPAL SYMPTOMS TIME OF INCUBATION DURATION OF DISEASE TEMPERATURE AT WHICH ORGANISM IS DESTROYED STAPHYLOCOCCUS AUREUS Gram positive coccus Hands, hair, nose, animals, septic spots, boils Aerobic and faculatively anaerobic Cooked meats, poultry, egg products, canned products, milk, cream Handling of food and equipment Nausea, vomiting 2-6 hours 24-48 hours 70°C for 2 minutes toxins are thermostable CLOSTRIDIUM PERFRINGENS Gram positive, spore forming bacillus Faeces, raw meat, poultry, soil Anaerobic and microerophilic Cooked meats, poultry, stews, gravies, canned meats handling of food and equipment, soil Abdominal cramp, diarrhoea 8-24 hours 24-48 hours Spores are resistant to boiling SALMONELLA SPECIES Gram negative rod Human and animal intestines, water bourne Aerobic and faculatively anaerobic Any food of nimal origin, seafood, egg, chocolate Live and slaughtered animals, handling of equipment Acute gastoenteritis, fever, diarrhoea, vomiting, septicaemia 12-72 hours 2-5 days 70°C for 2 minutes ESCHERICHIA COLI Gram negative rod Human and animal intestines Faculatively anaerobic Meats and derivatives, cheese, milk products Handling of food and equipment Diarrhoea, dysentry 6-36 hours 1-7 days 70°C for 2 minutes VEROCYTOTOXIN (VTEC) PRODUCING ESCHERICHIA COLI 0157 Gram negative rod Faeces, raw meat, raw milk Aerobic and faculatively anaerobic Meats, minced meats and derivatives, yogurt, raw milk Slaughtered animals, handling of equipment Severe abdominal pain, watery diarrhoea with blood, vomiting, little or no fever, may cause HUS leading to kidney dailure and anaemia 3-9 days 6-8 days Mortality rate 3-5% in cases with HUS 70°C for 2 minutes CLOSTRIDIUM BOTULINUM Gram positive, spore forming bacillus Soil, water Anaerobic Canned or preserved foods External contamination of equipment Nausea, vomiting, paralysis 10-48 hours Approx 10 days – however mortality rate is high Spores are resistant to boiling CAMPYLOBACTER SPECIES Gram negative bacillus Faeces, live animals, water Faculatively anaerobic and microaerophilic Poultry, unpasteurised milk Slaughtered animals Fever, diarrhoea, abdominal cramp 2-11 days 3-14 days 70°C for 2 minutes LISTERIA SPECIES Gram positive bacillus Ubiquitous Aerobic and faculatively anaerobic Fish, chicken,cheeses, raw foods General environment Heavy cold, diarrhoea, septacaemia 1-3 days 3-21 days 70°C for 2 minutes ROTAVIRUSES RNA virus double strand Faeces, polluted water Cell culture in specific host cells No specific food Handling of food, polluted water Vomiting, diarrhoea, upper respiratory tract infections, may also cause fever 1-7 days (2-4 days typical) 2-3 days 70°C for 2 minutes NORO VIRUS Believed to be RNA virus single strand Faeces, polluted water Cell culture in specific host cells Shellfish Handling of food, polluted water Nausea, vomiting, abdominal pain, malaise, low grade fever 24-72 hours 24-48 hours 80°C for 2 minutes HEPATITIS A RNA virus single strand Faeces, polluted water Cell culture in specific host cells Contaminated water, raw vegetables, shellfish Handling of food (faecal-oral route) polluted water Fever, malaise, abdominal pain, jaundice, liver may be affected 15-50 days 1 week to several months 80°C for 2 minutes BACILLUS CEREUS Gram positive bacillus sporeforming Soil, dust, water, intestines Aerobic and faculatively anaerobic Vegetables, meats, cereals, milk General environmental Gastroenteritis, vomiting, diarhoea 1-6 hours 6-24 Hours 90°C for 2 minutes SPOILAGEMICRO-ORGANISMS LACTOBACILLUS SPECIES Gram positive bacillus Ubiquitous, mouth, gastrointestinal tract, widely found in nature Aerobic some anaerobic Dairy, grain, meat, fish and derived products, water sewage, beer, wine, fruit Plant material, part of normal gut flora Little clinical significance, pathogenicity is rare Spores are resistant to boiling ZYGOSACCHAROMYCES BAILII Yeast Environment, normal flora of foods Aerobic and faculatively anaerobic High salt, low preservative, low water content squashes and mayonnaise Inadequately cleaned food processing plant, air bourne dust Little clinical significance, pathogenicity is rare. Causes post packing fermentaion of food products 70°C for 2 minutes Some species can survive pasteurisation PSEUDOMONAS SPECIES Gram negative rod Water, soil Aerobic Meat, poultry, seafood Water Infections where moisture accumulates (e.g. wounds, burns, urinary tract) 70°C for 2 minutes RHODOTORULA SPECIES Yeast Air, dust Aerobic and faculatively anaerobic Fresh and refrigerated meat, poultry, fish, seafood Air bourne dust Little clinical significance, pathogenicity is very rare. Produces pink pigments on meat, fish and poultry 70°C for 2 minutes SACCHAROMYCES SPECIES Yeast Stored grains, air, dust, fruit and vegetables Aerobic and faculatively anaerobic Beer, bakery goods Air bourne dust Little clinical significance, pathogenicity is very rare. Produces off flavours in fermented products 70°C for 2 minutes