Micro Coriolis air sampler

BEST PRACTICES FOR
ENVIRONMENTAL DETECTION
AND MONITORING OF AIRBORNE
VIRUSES WITH CORIOLIS AIR
SAMPLERS

Dr. Martin Serrano Sanchez, Field Application Scientist
at Bertin Technologies (AM session)
Dr. Floriane Cohen, Field Application Scientist at Bertin
Technologies (PM session)
Sophie Dubacq, Product Manager at Bertin
Technologies
I. INTRODUCTION: PRESENTATION OF
CORIOLIS AIR SAMPLERS
II. TIPS & TRICKS TO COLLECT VIRUS
III. CASE STUDIES: CORIOLIS FOR AIRBORNE
VIRUS DETECTION
Q&A SESSION
AGENDA OF THE SESSION

WEBINAR RULES
Your microphone will be muted
during the presentation to minimize
the interferences and back ground
noises.
If you have questions or remarks
during the presentation, please write
it in the question box.
After the presentation, speakers will
answer to your questions.

INTRODUCTION: PRESENTATION OF
CORIOLIS AIR SAMPLERS

OPTIMIZED SOLUTION FOR LIFE SCIENCES
15 years of expertise in designing and manufacturing
high quality, innovative & ergonomic lab equipment
Precellys
Universal tissue
Homogenizer
InCellis
Smart
Cell imaging System
BioReagent
ELISA kits
Coriolis µ
Air Sampler for
bio-contamination
quality control

CORIOLIS® µ
SINCE 2009
Air Sampler for bio-contamination
quality control
FRENCH ARMY PROJECT
SINCE 2006
Portable Air Sampler for airborne
pathogens detection
MONALISA PROJECTS
2006-2009
Validation of a new method for
pollen and allergen detection
10 YEARS OF WET CYCLONE TECHNOLOGY IMPLEMENTATION
CORIOLIS COMPACT
SINCE 2020
Compact and lightweight Air
Sampler for bio-contamination
quality control

CORIOLIS: APPROVED BY KOL WORLDWIDE

CORIOLIS µ PRODUCT DESCRIPTION
Dimensions: 220 x330 x 360 mm
Weight: 2,8 kg
Noise: 70 dBa at 300 L/min
Usage temperature: between + 5 /+ 40 ° C
Autonomy : 1h on battery, up to 6h with Long Time Monitoring
Battery: NiMH
Air Flow: 100-300 L/min
Collect any biological sample
Use: indoor & outdoor

CORIOLIS WET CYCLONE TECHNOLOGY
Air
Collection liquid
Particles
Bacteria, fungi, spores,
viruses, pollens, allergens,
endotoxins...
Pre-filled sterile cone with
adapted collection liquid
Air & particles enter into the cone
and form a vortex: aspirated
particles are centrifuged with the
collection liquid on the wall
Particles are concentrated into
the collection liquid

CORIOLIS µ PRODUCT DESCRIPTION
Main parts:
1. Coriolis body
2. Cane
3. Air Intake
4. Cone

NEW: CORIOLIS COMPACT - DRY CYCLONIC
Dimensions: 255 x135 x 130 mm
Weight: 1,42 kg
Noise: 62 dBa
Usage temperature: between + 5 /+ 45 ° C
Autonomy: 8H
Battery: Li-Ion
Air Flow: 50 L/min
Collect any biological sample
Use: indoor & outdoor
Bluetooth

CORIOLIS COMPACT: DRY CYCLONIC TECHNOLOGY
Aspirated air
goes through the
cone
Particles are
retained in the
cone wall
Collected particles
can be recovered by
rinsing the cone.
Cone is placed
on the device.

CORIOLIS COMPACT HIGHLIGHTS
Collect more particles with up to 8h hours
straight sampling.
Sample compatible with rapid analysis
methods (qPCR).
No need of concentrate sample.
Easy to transport, can be put in strategic
places or even on a drone.

CORIOLIS COMPACT PRODUCT DESCRIPTION
Main parts:
1. Coriolis body
2. Air Intake
3. Cone
1
2
3

COMPATIBLE WITH BIOLOGICAL ANALYSIS
Culturing methods
 Based on the study of the main phenotypic
characteristics of bacteria
Immunological methods
 Based on the recognition of a specific antigen of
the agent
Genetic methods
 Based on the recognition of a specific nucleic
acid sequence of the agent
Spectrometry methods
 Physical methods based on the recognition of
molecular structures by their mass

ENVIRONMENTAL MONITORING OF AIRBORNE VIRUSES
HEALTH
VIRUS DETECTION IN
HOSPITALS AND
NURSING HOMES
FOOD INDUSTRY
SARS-CoV-2,
BACTERIOPHAGES
AFFECTING PRODUCTION
VETERINARY
MONITOR VIRAL
CONTAMINATION OF
LIVESTOCK IN FARMS
OFFICE SPACES
DECONTAMINATION
CONTROL DURING
PANDEMICS

TIPS AND TRICKS SESSIONS TO COLLECT
VIRUS IN THE AIR

WORKFLOW: FROM SAMPLING TO ANALYSIS
SAMPLING
STRATEGY
SAMPLE
COLLECTION
SAMPLE
STORAGE
SAMPLE
PROCESSING
SAMPLE
ANALYSIS
DECONTAMI-
NATION
PROCEDURES
Choose
a sampling
strategy adapted
to the
environment
(sampling room
size, air flow
patterns..)
Use
appropriate
parameters for
airflow rate
and sampling
cycles duration
Store
and transport
samples in
appropriate
conditions to avoid
RNA degradation
Optional
reconcentration
step
Get
reliable results in
hours with rapid
microbiology
techniques
Decontaminate
the Coriolis after
each experiment

BEST PRACTICES
SAMPLING STRATEGY
 Air flow rates: Bertin recommends an airflow rate of 200 L/min but we have
ran 300 L/min using the Coriolis µ, 50L/min with the Coriolis Compact
 Sampling Duration: Minimum sampling time is 20-30 min
 Device Position: Start from the furthest distance away and move closer to
the patient. The device should always be placed on the trajectory of the
airflow in the room.
 Sampling Liquid: The optimal collection liquid for virus-laden aerosols
sampling is Phosphate Buffer Saline buffer. A culture medium such as DMEM
or MEM is also a suitable alternative. On the other hand, most RNA shields
should be avoided due to high evaporation speeds. The recommended
starting sampling volume liquid range is between 5 and 15mL.

BEST PRACTICES (2)
SAMPLE STORAGE
 Before transportation, samples should be transferred from the cones into
appropriate storage tubes. Samples can be stored for up to 24h at 4°C. For
long term storage, they can be frozen in cryotubes at -20°C or -80°C.
SAMPLE PROCESSING
 Coriolis µ:
• Reconcentration step with a tangential flow filtration device, such as the Amicon
100 kDa Amicon Ultra-15 (Millipore)
• Or choose a small starting volume of collection liquid (such as 5mL), then taking a
small aliquot of around 150 μL for DNA or RNA extraction.
 Coriolis Compact
• Resuspend in the desired volume by using a swab or vortexing the cone

BEST PRACTICES (3)
SAMPLE ANALYSIS
 Before transportation, samples should be transferred from the cones into
appropriate storage tubes. Samples can be stored for up to 24h at 4°C. For
long term storage they can be frozen in cryotubes at -20°C or -80°C.
DECONTAMINATION
 Cane, air intake and sampling cone
• Autoclave (except cone for Coriolis Compact)
• Soak in commercial bleach solution
• Ethanol 70%
 Instrument
• H2O2 vapor
• Run them with an ethanol-filled cone for 15min at maximum speed
* All references are available on the White paper called best practices for environmental detection and monitoring of airborne viruses with Coriolis® air samplers

VIRUS DETECTION WITH CORIOLIS AIR SAMPLERS
CASE STUDIES
SARS-CoV-2 DETECTION with Coriolis® µ air sampler
NOROVIRUS DETECTION IN HOSPITALS with Coriolis® µ
SWINE INFLUENZA A VIRUS DETECTION IN PIG FARMS with Coriolis® μ
EVALUATION OF DECONTAMINATION PROCEDURE IMPACT IN
DAIRY FACTORIES with Coriolis® Compact

Thanks to Jie Zhou to share the results and authorize us to present it.
SARS-COV-2 DETECTION WITH CORIOLIS µ AIR SAMPLER

Objective of the study: To evaluate SARS-CoV-2 surface and air contamination during
the peak of the COVID-19 pandemic in London.
Sampling design: Surface and air samples were collected in 8 different sites including 7
clinical areas and 1 public area at a North Western London hospital. In each area, 4 air
samples were collected Surface samples were collected by swabbing 25 cm2 of items
Collection protocol: Sampling was carried out with the Coriolis μ air sampler (Bertin
Technologies, France) at 100L/min for 10 min (corresponding to 1m3 of air), with 5 mL
DMEM
Analysis: RT-qPCR and viral culture
SARS-COV-2 DETECTION WITH CORIOLIS µ AIR SAMPLER

RESULTS
Viral RNA detected in 114/218 (52.3%) of
surface samples
Viral RNA detected in 14/31 (38.7%) air
samples
PCR results showed viral RNA concentration too
low for the virus to be cultivable
Viral RNA detected in public areas of the
hospital
PCR results from air samples.
From: Zhou, J. (2020). Investigating SARS-CoV-2 surface and air contamination in an
acute healthcare setting during the peak of the COVID-19 pandemic in London.
Medrxiv.

NOROVIRUS DETECTION IN HOSPITALS
Bonifait, Laetitia, et al. "Detection and quantification of airborne norovirus during outbreaks in healthcare
facilities." Clinical infectious diseases 61.3 (2015): 299-304.
Motivation: Noroviruses are a risk for patients in
hospitals: responsible for 50%of gastroenteritis
outbreaks worldwide
Goal: Investigate the presence of norovirus GII
bioaerosols during gastroenteritis outbreaks in
healthcare facilities

NOROVIRUS DETECTION IN HOSPITALS: PROTOCOL
Sampling strategy: 48 samples in 8 hospitals during outbreaks
 1m from each patient
 in front of patient’s room
 at the nurses’station
Collection: Coriolis μ, 200L/min during 10min in 15mL PBS
Sample processing: Filtration and concentration of the sample with
Amicon device (Millipore)
Sample analysis: viral RNA extraction , RT-qPCR

NOROVIRUS DETECTION IN HOSPITALS: RESULTS

SWINE INFLUENZA A VIRUS DETECTION IN PIG FARMS
Cador, C. Hervé, S. Andraud, M. Gorin, S. Paboeuf, F. Barbier, N. Quéguiner, S. Deblanc, C. Simon, G. Rose,
N. (2016). Maternally-derived antibodies do not prevent transmission of swine influenza A virus between
pigs. Veterinary Research 47:86.
Motivation: Understanding Swine influenza A virus (swIAV)
transmission through aerosols
Goal: Determine the relationship between airborne swIAV
detection and the number of infected pigs in pig farms
swIAV detection in air samples collected in experimental
rooms housing specific-pathogen-free (SPF) pigs without or
with maternally-derived antibodies (MDA)

SWINE INFLUENZA A VIRUS DETECTION IN PIG FARMS
Experimental design:
33 MDA- piglets were assigned to 3 independent rooms (rooms 1 to 3)
33 MDA+ piglets to 3 independent rooms (rooms 4 to 6)
In each room :
- 2 seeder pigs (inoculated with swIAV)
- 4 pigs in direct contact with seeder
pigs (same pen)
- 5 pigs in indirect contact with seeder
pigs ( different pen 30cm apart)

SWINE INFLUENZA A VIRUS DETECTION IN PIG FARMS:
PROTOCOL
Sampling strategy:
Coriolis μ air sampling between 2 pens, 70 cm
away from the ground at the height of pigs but
without direct contact with them
Nasal swabs
Collection: 300L/min during 10min in 15mL PBS with 0.005% Triton
3 times a week for 25 days post-infection
Sample processing: Filtration and concentration of the sample with
Amicon device (Millipore)
Sample analysis: viral RNA extraction , RT-qPCR

SWINE INFLUENZA A VIRUS DETECTION IN PIG FARMS:
RESULTS
swIAV genome was detected in aerosols from all rooms, peak at DPI 9
all indirect-contact (IC) pigs shown to be infected

EVALUATION OF DECONTAMINATION PROCEDURE IMPACT
IN DAIRY FACTORIES
Motivation: phage infection of lactococcal bacteria can
hinder fermentation processes > delay in cheese
production, variation in taste and texture
Goal: Evaluate the impact of ozone decontamination
treatment on the bacteriophages present in the air in a
dairy factory.

IN DAIRY FACTORIES: PROTOCOL
Decontamination: Alphatech Mobil disinfection unit C28 N°439
Ozone (O3) concentration target 2ppm/2h during 8h
Sampling strategy: 4 sampling times + control sample
 before ozone treatment
 just after ozone treatment
 2 days after ozone treatment
 after 2nd ozone treatment
Collection: Coriolis Compact 50L/min during 5 hours
Analysis: DNA extraction, simplex PCR specific for Lactococcus
lactis phages (limit of detection 101 to 102 phages / ml)
Coriolis Compact on dairy plant facilities.
5h at 50 l/min

IN DAIRY FACTORIES: RESULTS
C2 phages /ml SK1phages /ml PP35 phages /ml
Control 0 0 0
Before O3 treatment 1000 100000 1000
Just after O3 treatment 10 1000 10
2 days after O3 treatment 100000 100000 1000
After second O3 treatment 10 100000 0
important impact of ozone treatment on C2 bacteriophages
SK1 bacteriophages population harder to reduce, 2nd treatment has no impact

CONCLUSION
Innovative and comprehensive systems
 Coriolis μ air sampler
• High Air Flow Rate
• LTM Platform
• Liquid sampling & Molecular analysis
 Coriolis Compact air sampler
• Easy to use and to transport on the field
• Dry sampling, no resuspension step before molecular analysis
Ideal instruments for airborne viruses detection

YOUR POINT OF CONTACT
Your sales representative:
 Sophie DUBACQ
 sophie.dubacq@bertin.fr
 +33 (0) 139 306 133
Customer service:
 Order-life@bertin.fr
 +33 (0) 139 306 036
Product manager:
sophie.dubacq@bertin.fr

Micro Coriolis air sampler

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