The document describes the Maxwell 16 system, a cartridge-based purification system that automates nucleic acid extraction. It can isolate genomic DNA, total RNA, and proteins. Samples that can be used include cultured cells, tissues, blood, bacteria, and food. The system uses magnetic beads to bind, purify, and isolate the targets. It provides high yields of pure nucleic acids and proteins in an automated workflow that is faster and more consistent than manual methods.

1. What can Maxwell® 16 do? WhiteSci What happens inside? What is Maxwell 16? What else can Maxwell 16 do? Plant Tissues & Seeds Cultured cells & bacteria

2. What can Maxwell® 16 do? WhiteSci What happens inside? What else can Maxwell 16 do? Special Offer Plant Tissues & Seeds Cultured cells & bacteria Close What is Maxwell® 16? Imagine if your gDNA preps consisted of: Placing a sample into a cartridge Pushing a button Walking away Returning ~45 minutes later to high yield, pure gDNA ready for assay! The Maxwell 16 System is a cartridge-based purification system bringing automation to your benchtop. Change applications by changing cartridges and selecting a different program. Maxwell 16 can bring better results to your laboratory thanks to improvements in purity and consistency.

3. What can Maxwell® 16 do? WhiteSci What is Maxwell 16? What else can Maxwell 16 do? Special Offer Plant Tissues & Seeds Cultured cells & bacteria Close What happens inside? Maxwell® 16 is a magnetic particle processor, not a liquid handler. The sample is mixed by rapid up-and-down movement of the single-use plunger. The MagneSil® Paramagnetic Particles are captured after a magnetic rod insert down the middle of the plunger. The MagneSil Particles bind the nucleic acid The nucleic acids are purified through a series of capture and release washes The purified nucleic acid is released. Maxwell 16 ® Tissue Genomic DNA Cartridge 2 3 4 5 6 7 1 3 4 5 6 7 2 3 4 5 6 7 2 1 4 5 6 Mixing Capture Binding Washing & Elution 300µl

4. What can Maxwell® 16 do? WhiteSci What happens inside? What is Maxwell 16? What else can Maxwell 16 do? Special Offer Plant Tissues & Seeds Cultured cells & bacteria Close What else can Maxwell® do? Low Elution Volume (LEV) gDNA Standard Elution Volume (SEV) & LEV Total RNA SEV Polyhistidine Protein Purification SEV? LEV?

5. What can Maxwell® 16 do? 300-600µl 30-100µl WhiteSci What happens inside? What is Maxwell 16? What else can Maxwell 16 do? Special Offer Plant Tissues & Seeds Cultured cells & bacteria Close What else can Maxwell® do? Low Elution Volume (LEV) gDNA Standard Elution Volume (SEV) & LEV Total RNA SEV Polyhistidine Protein Purification Close 1 3 4 5 6 7 2 2 3 4 5 6 1 Maxwell® 16 can work with two formats of cartridges. SEV & LEV Standard Elution Volume (SEV) Low Elution Volume (LEV) Maxwell® 16 Instrument can switch from SEV to LEV format or back in less than 5 minutes.

6. What can Maxwell® 16 do? WhiteSci What happens inside? What is Maxwell 16? Special Offer Plant Tissues & Seeds Cultured cells & bacteria Back Low Elution Volume gDNA Process 1-10 5µm formalin-fixed paraffin-embedded sections after overnight proteinase K digest. No xylene extractions. Maxwell ® 16 FFPE Tissue LEV DNA Purification Kit Cat. # AS1130 Maxwell ® 16 Cell LEV DNA Purification Kit Cat. # AS1030 Maxwell ® 16 LEV Total Nucleic Acid Purification Kit Coming Soon! Designed for 10-10,000 cells. Ideal for capturing gDNA from samples like stem cell colonies, cerebral spinal fluid, etc. Currently in testing for viral total nucleic acid purification More information in the Clinical Section of http://www.maxwell16.com/ Close

7. What can Maxwell® 16 do? WhiteSci What happens inside? What is Maxwell 16? Special Offer Plant Tissues & Seeds Cultured cells & bacteria SEV & LEV Total RNA Maxwell ® 16 Total RNA Purification Kit Cat. # AS1050 Maxwell ® 16 Tissue LEV Total RNA Purification Kit Cat. # AS1220 Back Close Maxwell ® 16 Cell LEV Total RNA Purification Kit Cat. # AS1225 Maxwell ® 16 Tissue LEV 28S 18S M 1 2 3 4 5 6 1 Brain 2 Heart 3 Kidney 4 Liver 5 Lung 6 Spleen 1µg loaded, denaturing gel Samples are homogenized & processed to remove gDNA prior to addition to the cartridge and purification.

8. What can Maxwell® 16 do? WhiteSci What happens inside? What is Maxwell 16? Special Offer Plant Tissues & Seeds Cultured cells & bacteria SEV & LEV Total RNA Maxwell ® 16 Polyhistidine Protein Purification Kit Cat. # AS1220 Back Close Purifies polyhistidine-tagged protein from bacteria, insect cells, mammalian cells, cell-free expression reactions, conditioned cell culture media, etc. Total Wash Eluate HQ-tagged MonsterGreen™ Fluorescent Protein expressed in S30 T7 High Yield Expression System

9. B = Mouse Brain H = Mouse Heart I = Mouse small intestine L = Mouse Liver P = Mouse pancreas S = Mouse spleen T 1 = 1cm mouse tail T 0.5 = 0.5 cm mouse tail 5µl gDNA eluate 1µl gDNA eluate in PCR Quick Isolation from a variety of tissues Maxwell 16 ® Tissue DNA Purification Cartridge 2 3 4 5 6 7 Tissue Sample Mouse tails and other animal tissue are homogenized directly in lysis buffer without Proteinase K. WhiteSci Maxwell ® 16 Tissue DNA Purification Kit Cat. # AS1030 48 preps gDNA from Model Organisms gDNA from Food Samples Back

10. B = Mouse Brain H = Mouse Heart I = Mouse small intestine L = Mouse Liver P = Mouse pancreas S = Mouse spleen T 1 = 1cm mouse tail T 0.5 = 0.5 cm mouse tail 5µl gDNA eluate 1µl gDNA eluate in PCR Quick Isolation from a variety of tissues Maxwell 16 ® Tissue DNA Purification Cartridge 2 3 4 5 6 7 Tissue Sample Mouse tails and other animal tissue are homogenized directly in lysis buffer without Proteinase K. WhiteSci Maxwell ® 16 Tissue DNA Purification Kit Cat. # AS1030 48 preps Use Maxwell® 16 to speed research with Model Organisms More information online http://www.promega.com/maxwell16/applications Close Source Yield (PicoGreen ® ) C t (1µl eluate) C t (5µl eluate) Target Drosophila melanogaster 0.32µg (1 fly) 1.52µg (5 flies) 24.4 21.5 GAPDH gene Danio rerio 24.8µg 50mg tissue 26.7 24.5 p53 gene Caenorhabditis elegans 0.08µg ~50,000 whole worms not detected 34.4 Laminin Saccharomyces cerevisiae 0.72µg 3mm colony with lyticase pre-treatment 25.3 21.5 LYS2 gDNA from Food Samples Back

11. B = Mouse Brain H = Mouse Heart I = Mouse small intestine L = Mouse Liver P = Mouse pancreas S = Mouse spleen T 1 = 1cm mouse tail T 0.5 = 0.5 cm mouse tail 5µl gDNA eluate 1µl gDNA eluate in PCR Quick Isolation from a variety of tissues Maxwell 16 ® Tissue DNA Purification Cartridge 2 3 4 5 6 7 Tissue Sample Mouse tails and other animal tissue are homogenized directly in lysis buffer without Proteinase K. WhiteSci Maxwell ® 16 Tissue DNA Purification Kit Cat. # AS1030 48 preps gDNA from Model Organisms Close Directly process food samples to detect… milk cheeses Panel A: S. aureus presence in milk and cheese samples Panel B: Multiplex PCR for S. aureus toxins … bacteria in dairy products Amplification of 18S bovine rRNA and 16S bacterial rRNA . … bacteria in raw meat samples Data courtesy of P. Leone and P. Cremonesi, Italian National Council of Research-Instititute of Agro-Biology and Biotechnology More information online: www.promega.com/maxwell16/applications Back

12. Isolate gDNA from good or “bad” blood Maxwell 16 ® Blood DNA Purification Cartridge 2 3 4 5 6 1 Anti-coagulated whole blood or buffy coat cells added directly to Reagent Cartridge WhiteSci Maxwell ® 16 Blood DNA Purification Kit Cat. # AS1010 48 preps  400 300 200 100 75 50 µl fresh whole blood +4°C -20°C RT Storage Condition Apoptotic DNA  Buffy Coat Data Concordance Study Back

13. Isolate gDNA from good or “bad” blood Maxwell 16 ® Blood DNA Purification Cartridge 2 3 4 5 6 1 Anti-coagulated whole blood or buffy coat cells added directly to Reagent Cartridge WhiteSci Maxwell ® 16 Blood DNA Purification Kit Cat. # AS1010 48 preps  400 300 200 100 75 50 µl fresh whole blood +4°C -20°C RT Storage Condition Apoptotic DNA  Concordance Study Close Using buffy coat cells gives higher gDNA yields and concentrations Buffy Coat gDNA >100ng/µl Back

14. Isolate gDNA from good or “bad” blood Maxwell 16 ® Blood DNA Purification Cartridge 2 3 4 5 6 1 Anti-coagulated whole blood or buffy coat cells added directly to Reagent Cartridge WhiteSci Maxwell ® 16 Blood DNA Purification Kit Cat. # AS1010 48 preps  400 300 200 100 75 50 µl fresh whole blood +4°C -20°C RT Storage Condition Apoptotic DNA  Buffy Coat Data Close PCR results concordant to reference extraction method 16 samples amplified, analyzed by gel electrophoresis Data courtesy: Dr. Deborah O. Crowe, PhD, D(ABHI) DCI Laboratory, Nashville, TN Reference Manual Purification Method Maxwell® 16 400µl whole blood Maxwell® 16 300µl buffy coat cells

15. Quick gDNA isolation from cultured cells Maxwell 16 ® Cell DNA Purification Cartridge 2 3 4 5 6 1 Directly process Cultured mammalian cells Gram negative bacteria Pre-treat Gram positive bacteria with lysozyme HeLa 1x10 6 cells Designed for 1 x 10 5 to 5 x 10 6 mammalian cells 3T3 CHO 293 WhiteSci Maxwell ® 16 Cell DNA Purification Kit Cat. # AS1020 48 preps Gram Negative Bacteria Gram Positive Bacteria Back

16. Quick gDNA isolation from cultured cells Maxwell 16 ® Cell DNA Purification Cartridge 2 3 4 5 6 1 Directly process Cultured mammalian cells Gram negative bacteria Pre-treat Gram positive bacteria with lysozyme HeLa 1x10 6 cells Designed for 1 x 10 5 to 5 x 10 6 mammalian cells 3T3 CHO 293 WhiteSci Maxwell ® 16 Cell DNA Purification Kit Cat. # AS1020 48 preps Gram Positive Bacteria Close Genomic DNA isolation from a variety of Gram negative bacteria 400µl overnight culture Escherichia coli Pseudomonas aeruginosa Proteus vulgaris Salmonella typhimurium Yersinia enterocolitica 1 2 3 4 5 1 2 3 4 5 Designed to work with up to 2 x 10 9 bacterial cells

17. Quick gDNA isolation from cultured cells Maxwell 16 ® Cell DNA Purification Cartridge 2 3 4 5 6 1 Directly process Cultured mammalian cells Gram negative bacteria Pre-treat Gram positive bacteria with lysozyme HeLa 1x10 6 cells Designed for 1 x 10 5 to 5 x 10 6 mammalian cells 3T3 CHO 293 WhiteSci Maxwell ® 16 Cell DNA Purification Kit Cat. # AS1020 48 preps Gram Negative Bacteria Close Genomic DNA isolation from a variety of Gram positive bacteria 400µl overnight culture Staphylococcus aureus Arthrobacter luteus Bacillus subtilis Bacillus cereus Enterobacter faecalis 1 2 3 4 5 1 2 3 4 5 Cells treated with lysozyme prior to isolation Designed to work with up to 2 x 10 9 bacterial cells 400µl overnight culture Back

18. Maxwell 16 ® Tissue DNA Purification Cartridge 2 3 4 5 6 7 Leaf Punch Many plant samples can be processed directly. Others may require an enzymatic pre-treatment Improve the purity and consistency of your plant genomic DNA purification Isolation genomic DNA from eight samples of 25mg each of tomato leaf using the Maxwell 16 Instrument and the Maxwell 16 Tissue DNA Purification Kit. WhiteSci Maxwell ® 16 Cell DNA Purification Kit Cat. # AS1020 48 preps More Plant Samples Back

19. Maxwell 16 ® Tissue DNA Purification Cartridge 2 3 4 5 6 7 Leaf Punch Many plant samples can be processed directly. Other may require an enzymatic pre-treatment Improve the purity and consistency of your plant genomic DNA purification Isolation genomic DNA from eight samples of 25mg each of tomato leaf using the Maxwell 16 Instrument and the Maxwell 16 Tissue DNA Purification Kit. WhiteSci Maxwell ® 16 Cell DNA Purification Kit Cat. # AS1020 48 preps Close Example Yield and Purity with Maxwell® 16 Tissue DNA Purification Kit www.promega.com/maxwell/applications Plant Tissue Samples Yields determined with PicoGreen ® Reagent (Molecular Probes). Details available at www.promega.com/maxwell16/applications Samples digested with CelluACE™ XG System (Cat. # FF3800) in the presence of polyvinylpyrrolidone prior to isolation. Samples digested with CelluACE™ XG System (Cat. # FF3800) prior to isolation. Sample Size Yield 1 Detected by qPCR 2 Arabidopsis thaliana (mustard) 1 leaf 139ng + Brassica napus (canola) 5 seeds 2.3µg + 8mm punch 512ng + Fragaria ananassa (strawberry) 8mm punch 193ng 3 + Glycine Max (soybean) 1 seed 3.1µg 4 + 8mm punch 587ng + Gossypium hirsutum (cotton) 1 seed 2.6µg 4 + 8mm punch 854ng + Sample Size Yield 1 Detected by qPCR 2 Medicago sativa (alfalfa) 8mm punch 1.4µg + Oryza sativa (rice) 1 seed 216ng 4 + 5mm punch 238ng 4 + Solanum lycopersicum (tomato) 8mm punch 734ng + Triticum aestivum (wheat) 5mm slice 5.6µg 4 + 1 seed 1.4µg 4 + Vitis vinigera (grape) 8mm punch 20ng 3 + Zea mays (corn) 8mm punch 655ng + Back

20. Rapid Mouse Tail gDNA isolation… for less 5µl of gDNA from 8 x 1cm mouse tail snips Get Maxwell 16 into your lab, so you can Isolate tail gDNA sooner Genotype mice earlier Identify transgenics faster All without Proteinase K! WhiteSci Discover how simple your gDNA preps can be Ask for a Demo! Back