The document discusses a Light-CT scanner from LLTech that provides fast, high-resolution 3D imaging of tissue samples. The scanner uses optical coherence tomography to generate 1 μm resolution images across a 200 μm penetration depth in minutes, without damaging samples. It allows pathologists to examine breast tissue and identify structures like ducts and lobules. The scanner could help reduce unnecessary repeat surgeries by enabling rapid margin analysis and metastasis detection compared to traditional techniques.

1. LLTech
Light-­‐CT
Scanner
Breast
images
2012
© LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com 1

2. Light-­‐CT™
key
benefits
• Op8cal
in-­‐depth
biopsies
of
gross
8ssue
within
minutes
• 1
µm
2D
and
3D
histopathological
resolu8on
• Easy
explora8on,
acquisi8on
and
rendering
in
DICOM
format
• Safe,
non-­‐invasive
and
non-­‐destruc8ve
process
Fast
and
non-­‐invasive
3D
in-­‐depth
structural
and
cellular
imaging
© LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com 2

3. Light
Computed
Tomography
technology
Based
on
Full-­‐Field
Op8cal
Coherence
Tomography
(FFOCT)
Combines
microscope
resolu8on
with
interferometry
High
resolu8on
in-­‐depth
C
scans
Commercial
device
specs:
• Excellent
resolu8on:
1,5µm
transverse,
1µm
axial
• 70Hz
max.
tomographic
frame
rate
–
0.8
mm
x
0.8
mm
•
Penetra8on
depth
200µm
–
1mm
depending
on
8ssue
scaZering
• 25
mm
diameter
sample
size
• Small
footprint:
Scanner
and
light
source
fit
on
70
cm
x
35
cm
© LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com 3

4. Light-­‐CT™
Scanner
for
ex-­‐vivo
cellular
imaging
Op8cal
acquisi8on
unit,
• User
friendly
moving
ver8cally
acquisi8on
soware
• DICOM
2D
Movable
tray
and
3D
with
sample
Viewer
holder
X,Y
moving
stage
White
Light
Joys8ck
for
easy
Integrated
wide
field
camera
to
Source
control
of
X,Y,
Z
take
sample
picture
before
movements
imaging
© LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com 4

5. BREAST IMAGING
© LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com 5

6. Healthy
breast
<ssue
Grainy
aspect
of
normal
Duct
with
Lobule
Adipocytes
Vessel
fibrous
8ssue
calcifica8on
© LLTech 2012 © LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com Courtesy of Hôpital Tenon, Paris, France
6

7. Breast tissue reading tree
© LLTech 2012 © LLTECH 2011Diagnosis decision
t ree
for
FF-­‐OCT
images
o n
h uman
b reast
t issue
Figure
3 :
Copyright LLTech 2012 – www.lltechimaging.com 7

8. Breast Histology
DCIS – Ductal Carcinoma in situ
Fat cells
1 cm
Lobules
Breast., Ductal Carinoma H&E vs LightCT on Fresh Tissue
Hopital Tenon, France, August 2010
© LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com 8

9. Breast Histology
DCIS – Ductal Carcinoma in situ
Lobules
Duct with
necrosiss
© LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com 9

10. Breast Histology
Invasive adenocarcinoma - Nodular tumor
Healthy
8ssue:
grainy
medium
scaZering
fibers
Tumorous
8ssue:
highly
scaZering
thin
trabeculae
aspect
of
fibers
© LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com 10

11. Breast
Milk duct with thick wall
1 mm
3 mm
1
2
Connective Fat cells
tissue
1 mm
Blood vessels
Traditional Histology
Milk Duct
© LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com 11

12. Breast Histology
Identification of the milk duct structures
Milk Duct End
Fat Cells
© LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com 12

13. Healthy
breast
<ssue
Lobule
Galactophorous
duct
cut
longitudinally
Honeycomb
configura8on
of
adipocytes
© LLTech 2012 © LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com Courtesy of Hôpital Tenon, Paris, France
13

14. Nipple
-­‐
Cross
sec<on
Collec8ng
duct
with
secre8on
in
the
lumen
Collec8ng
duct
in
the
retroareolar
sec8on
© LLTech 2012 © LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com Courtesy of Hôpital Tenon, Paris, France
14

15. Invasive
adenocarcinoma
with
in
Situ
Component
Highly
scaZering
thin
trabeculae
Enlarged
ducts
filled
with
cells
aspect
of
fibrous
8ssue
prolifera8on
or
necrosis
surrounding
grey
cellular
zones
© LLTech 2012 © LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com Courtesy of Hôpital Tenon, Paris, France
15

16. Fibroadenoma
Well
delimited
nodule
with
lobulated
appearence
Elongated
compressed
ductules
with
slit-­‐like
lumen
© LLTech 2012 © LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com Courtesy of Hôpital Tenon, Paris, France
16

17. Fibroadenoma
Enlarged
ductules
characteris8c
of
the
lesion
© LLTech 2012 © LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com Courtesy of Hôpital Tenon, Paris, France
17

18. The Light-CT scanner allows fast tissue Processing and
Pathology Examination. It completes the tools available for
pathologists
Tissue Scanner 3D
Biopsy Digital Image
5-8 minutes
Non-Destructive
2D
Digital Image
Slide
15-12 mins
Artefacts When Frozen Section is
Destructive needed
(Mostly Intra-operative)
12-24 hours
Expensive When Chemical Fixation
Destructive is needed
(Mostly Diagnostic)
© LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com 18

19. The Light-CT could help reducing the number of re excision in
breast cancer surgery whilst preserving the current habits of
pathologists
Up to 40% of double procedures Per surgery Post surgery
Up to 40% of double surgical
procedures
cancerous Cancerous
Current techniques
Histology
Visual
And/or Further
Surgery analysis,
OK Immuno OK treatment
cryostat
histochemistry
Node
Poor identification of
metastasis
No surgical
margins analysis
Margin
Few minutes analysis Several days analysis
Surgeon Pathologist Pathologist
Per surgery Post Surgery
Decrease of double surgical
Cancerous procedures Cancerous
Significative decrease of
Histology
double procedures
LLTech’s And/or Further
LLTech Light-CT
Surgery
analysis OK Immuno OK treatment
histochemistry
Surgical margin analysis
Metastasis analysis (> 2mm) et
micro metastaisi :
PN1mi > 0.2 – 2 mm <
Few minutes analysis Several days analysis
Surgeon Pathologist Pathologist
© LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com 19

20. Contact Details
USA Europe
LLTech Inc.
LLTech SAS
103 Carnegie Center Drive
6, place de la Madeleine
Suite 300
75008
Princeton, NJ 08540
Paris - France
USA
www.lltechimaging.com
www.lltechimaging.com
contact@lltech.fr
contact@lltech.fr
Phone :+33 9 72 16 33 40
Phone :+ 1 609 955 3506
© LLTECH 2011 Copyright LLTech 2012 – www.lltechimaging.com 20