Common name : Rice Scientific name : (Oryza sativa L.) Rice has become a model cereal plant for molecular genetic research. Rice has the most comprehensive molecular linkage maps with more than 2000 DNA markers. Linkage maps Linkage maps are the maps of the genes on a chromosome that based on linkage analysis. A linkage map does not show the physical distances between genes but rather their relative positions, as determined by how often two gene loci are inherited together. Diploid oryza has 24 chromosomes, and no of linkage grp is similar to the haploid no of chromosome of particular species. Number of Chromosome (2n) = 24 Number of linkage groups (n) = 12 Number of surveyed markers = 211 Number of markers = 170 Used Marker types = RFLP Mapping population = F1 population Since the establishment of 12 linkage groups corresponding to the haploid chromosome number of rice by Nagao and Takahashi (1963), have been investigating the relationships between the linkage groups and chromosomes mainly by using trisomics and reciprocal translocations (Iwata and Omura 1971a, )

1. Linkage Map Of
(Oryza sativa L.)
Dinithi Vihanga De Silva

2.  Common name : Rice
 Scientific name : (Oryza sativa
L.)
Rice has become a model
cereal plant for molecular genetic research.
Rice has the most comprehensive molecular
linkage maps with more than 2000 DNA
markers.
Linkage Map Of
(Oryza sativa L.)

3. What are the
linkage maps
?? Linkage maps
Linkage maps are the maps of
the genes on a chromosome that
based on linkage analysis. A
linkage map does not show the
physical distances between
genes but rather their relative
positions, as determined by how
often two gene loci are inherited
together.

4. Diploid oryza has 24 chromosomes, and no of linkage grp is
similar to the haploid no of chromosome of particular species.
Number of Chromosome (2n) = 24
Number of linkage groups (n) = 12
Number of surveyed markers = 211
Number of markers = 170
Used Marker types = RFLP
Mapping population = F1 population
Since the establishment of 12 linkage groups
corresponding to the haploid chromosome number of rice by
Nagao and Takahashi (1963), have been investigating the
relationships between the linkage groups and chromosomes
mainly by using trisomics and reciprocal translocations (Iwata
and Omura 1971a, )
Number of linkage groups
Figure 01- RFLP map with corrected orientation and
centromeric positions.

5. • Short arms of the chromosome are at the top and the long arms
of the chromosome are at the bottom. Then, solid lines are the
centromeric positions which are shown in blue arrows.

6. • Markers C711 and G103 (chromosome 9) and G1084 and G2155
(chromosome 10) ( which are marked with red arrows )are from the
map shown in figure 02. & Numbers at the top of each linkage
group refer to the chromosomes.

7. Figure 02 – markers
References : Kurata, N., Nagamura, Y.,
Yamamoto, K., Harushima, Y., Sue, N.,
Wu, J., Antonio, B.A., Shomura, A.,
Shimizu, T., Lin, S.Y. and Inoue, T., 1994.
A 300 kilobase interval genetic map of
rice including 883 expressed sequences.
Nature genetics, 8(4), pp.365-372.

8. Figure 03- Number of molecular markers
used for polymorphism
survey and arm location
References :Singh, K., Ishii, T., Parco, A.,
Huang, N., Brar, D.S. and Khush, G.S., 1996.
Chromosome 1. Of the
19 polymorphic probes
used for arm
location, 12 mapped on
the long arm and 7 on
the short arm.
Markers RZ288 and
RZ783, which map
toward distal regions
of the map, were located
on short and long arms,
respectively.
The centromere was
shown to lie between
RG811 and RZ413,
with RG811 on the short
arm and RZ413 on the
long arm.

9. Figure 4-.table of Plant material used for RFLP analysis
References :Singh, K., Ishii, T., Parco, A., Huang, N., Brar,
D.S. and Khush, G.S., 1996.

10.  Singh, K., Ishii, T., Parco, A., Huang, N., Brar, D.S. and Khush, G.S., 1996. Centromere mapping and
orientation of the molecular linkage map of rice (Oryza sativa L.). Proceedings of the National
Academy of Sciences, 93(12), pp.6163-6168.
 Kurata, N., Nagamura, Y., Yamamoto, K., Harushima, Y., Sue, N., Wu, J., Antonio, B.A., Shomura, A.,
Shimizu, T., Lin, S.Y. and Inoue, T., 1994. A 300 kilobase interval genetic map of rice including 883
expressed sequences. Nature genetics, 8(4), pp.365-372.
 Khush, G. S. (1973) Cytogenetics ofAneuploids (Academic, New York).
 Khush, G. S., Singh, R. J., Sur, S. C. & Librojo, A. (1984) Genetics 107, 141-163.
 O. Panaud. X. Chen. S. R. McCouch (1996) Development of microsatellite markers and
characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.)
References

11. Acknowledgement

12. Thank you…..