Alejandra Gómez Zuluaga Sofía Guerra Espinosa

1. ALEJANDRA GÓMEZ ZULUAGA – SOFÍA GUERRA ESPINOSA
UNIVERSIDAD PONTIFICIA BOLIVARIANA
TERCER SEMESTRE
2021

2. BREAST CANCER
Is the abnormal and disorderly proliferation
of malignant breast cells that leads to the
uncontrolled growth of a tumor in this tissue.
Breast cancer can spread outside the breast
through blood and lymph vessels. If it
spreads, the tumor is said to have
metastasized.
Epidemiology
• The highest incident occurs after age 35
with 84% of the cases occurring after
age 50.
• Lowest consumption of meat = lowest
incidence of breast cáncer
• Menarche and weight
• 1 in 12 women will develop Bca
sometime during their lifes.
• Leading cause of death and illness
among
Colombian women.
 PAX

3. LINC00337
1
Recently, the function of long
non-coding RNAs in tumor
development has been widely
understood, but their roles in
tumor- associated
macrophages are still in its
infancy.
2
Collectively, this study intends
to reveal the function and
possible mechanism of
LINC00337 in BCa through cell
experiments and animal
experiments combined with
bioinformatics analysis.
4
We demonstrate that IBC
strongly expresses IL8 and
growth-regulated oncogene
(GRO) chemokines that
activate STAT3, which
promotes development of high
levels of cancerigenous cells
and a mesenchymal
phenotype.
3
Another hallmark of Bca is
high infiltration of M2 tumor-
associated (immune-
suppressing) macrophages.
heyre commanded by the
cancerigenous cells to
become more tumor -
promoting.

4. Goal
● This study focused on the potential
involvement and mechanism of LINC00337
in Bca.

5. Materials and methods
RT – qPCR
It is a polymerase chain reaction that uses
reverse transcription. The letter “q” indicates
it´s a quantative test. It consists of the
amplification of a specific RNA sequence by
prior synthesis of its cDNA (DNA copy) using
a reverse transcriptase, followed by several
cycles of conventional PCR.

6. Materials and methods
ELISA
ELISA (enzyme – linked immunosorbent
assay) method was performed to assess the
levels of M-CSF and granulocyte-macrophage
colony stimulating factor (GM-CSF) in tumor
tissues or cell supernatants.

7. Materials and methods
Inmunohistochemestry
is the identification of a tissue by
means of a specific interaction
between an antigen and an antibody
where the antibody has been visibly
labeled.
IHC was constructed to determine the
expressions of CD163 and CD68 in
tumor tissues.

8. Materials and methods
WESTERN BLOT
Proteins from cell extracts are separated by
gel electrophoresis according to their size,
negatively charged. Then, the proteins are
transferred to a filter that is incubated with
antibodies that react with the protein of
interest.
ECL chemiluminescent solution and ImageJ
were performed for visualization and analysis.

9. RESULTS
BCa cells were cultured in a conditioned medium, the
functional test was carried out to detect the effect of
abnormal expression of LINC00337 in BCa cells. LINC00337
regulated the growth of breast cancer cells through M2
macrophages.

10. RESULTS
Overexpression of LINC00337 further
enhanced the effects of CM on promoting the
malignant phenotype of cells, while
shLINC00337 partially reduced the viability
and migration of BCa cells induced by CM.

11. RESULTS
1. LINC00337 promoted tumor
generation.
2. shLINC00337 and PAX inhibited
tumor growth.
3. PAX could partially reduce the
tumor size caused by LINC00337

12. RESULTS
It was detected the expression of IL-13 and CCL2 in
tumor tissues, and the results showed that the
expressions of IL-13 and CCL2 were down-regulated
in the shLINC00337 and vector + PAX groups, but
were up-regulated in the LINC00337 group.

13. AUTHOR THEIR WORDS DO THEY
AGREE?
Shi et al., 2020 “Recently, LINC00337 has been reported to have
cancer-promoting effects on a variety of cancers, such
as colorectal cancer and esophageal cáncer”
Huang et al.,
2017
“Macrophages are important immune cells, are their
functional diversity and differ- entiation also regulated
by lncRNA􏰉 The answer is yes”
Li et al., 2016 “studies have found that the differential expression level
of lncRNAs is closely related to the drug resistance of
tumors”
DISCUSSION

14. Molecular biology is fundamental in medicine
because it offers less invasive diagnostic
alternatives to the exploration, so as not to generate
metastases
In the research field, molecular biology is a great
contribution since it provides the means to find
solutions, not only for health problems, but also for
the prevention of diseases
CONCLUSIONS
Molecular biology contributes to the treatment of a
large number of infectious and genetically based
diseases that greatly affect the well-being of many
patients.

15. Alejandra Gómez Zuluaga

16. Sofía Guerra Espinosa