This document summarizes research on using nanoparticles for photothermal therapy on mice with cancer tumors. It includes figures showing the effects of different concentrations of nanoparticles and laser wavelengths on tumor temperature over time. The results show that injecting higher concentrations and doses of nanoparticles into tumors, followed by laser irradiation, led to greater increases in tumor temperature and helped shrink and eliminate tumors over time compared to lower doses.

1. Dan Feldman
“Nanonaps” for Photothermal therapy on Balb/C
mice with 4T1 (cancer cell line) tumors
7/6/15

2. ONC
F127Fig 1

3. 0
0.05
0.1
0.15
0.2
0.25
0.3
0.35
700 750 800 850 900
Absorbance
Wavelength (nm)
860 wavelength, before fridge
0
0.05
0.1
0.15
0.2
0.25
0.3
0.35
0.4
0.45
700 750 800 850 900
Absorbance
Wavelength (nm)
880 wavelength, after 24 hours in fridge

4. 866
868
870
872
874
876
878
880
882
884
0 0.5 1 1.5 2 2.5 3 3.5 4 4.5
Wavelength
DCM concentration (ml)
Wavelength effected by DCM
concentration
859
860
861
862
863
864
865
866
867
868
0 2 4 6 8 10 12 14 16
Wavelength(nm)
F127 concentration (ml)
Wavelength effected by F127
concentration
0 50 100 150 200
Pre Wash
Wash 1
Wash 2
Wash 3
Size (nm)
Pre Wash
Wash 1
Wash 2
Wash 3
Fig 2

5. Dye combinations
0
0.1
0.2
0.3
0.4
0.5
0.6
600 650 700 750 800 850 900 950 1000
Absorbance
Wavelength
250 ul ONC and 125 ul ZnBNc
0
0.1
0.2
0.3
0.4
0.5
0.6
600 700 800 900
Absorbance
Wavelength
250 ul ONc 250 ul ZnBNc
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
600 650 700 750 800 850 900 950 1000
absorbance
wavelength
250 ul ONC 500 ul ZnBNc
0
0.2
0.4
0.6
0.8
1
600 700 800 900 1000
absorbance
wavelength
500 ul onc and 250 ul znbnc
ZnBNc (707 nm) experiences a red shift when combined with ONc (860 nm)

6. 860 upconverting nanoparticles with Vaseline

7. 30
35
40
45
50
55
60
65
70
0 50 100 150 200 250 300
Temperature(Celcius)
Time (Seconds)
Temperature Vs. Time Of Different O.D.
0 O.D.
12.5 O.D.
25 O.D.
50 O.D.
Power Density = 550

8. 30
35
40
45
50
55
60
65
70
0 50 100 150 200 250 300
Temperature(Celcius)
Time (Seconds)
Temperature of Tumor
50 ul NNP
100 ul NNP
200 ul NNP
Tumor, 200 ul (100 OD),
0 seconds
Tumor, 200 ul (100 OD),
30 seconds
Tumor, 200 ul (100 OD),
1.5 minutes
PD= 400

9. 20 days after PTT
100 OD injection 50 OD injection

10. 0
0.02
0.04
0.06
0.08
0.1
0.12
0 10 20 30 40 50 60 70 80 90 100 110 120
Absorbance
Injected O.D.
Absorbance of nanonap after injection into tumor
0
0.5
1
1.5
2
2.5
3
3.5
x 10
4100 OD
0
0.5
1
1.5
2
2.5
3
3.5
x 10
450 OD
0
0.5
1
1.5
2
2.5
3
3.5
x 10
4Control, 0 OD
PAT images 24 hours after nanonaps were injected into the tail vein of mice

11. 0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0 20 40 60 80 100 120
MassofONc(mgdye/kgtumor)
Injected OD
Mass of dye in tumor
0
20
40
60
80
100
120
0 20 40 60 80 100 120
MeasuredO.D.aftertumorhomogenization
Injected O.D.
Measured O.D. in tumor

12. 100 OD Injected into mouse
0.5
1
1.5
2
2.5
3
3.5
x 10
4
0.5
1
1.5
2
2.5
3
3.5
x 10
4
At 860 nm laser At 820 nm laser