Customer discovery is a process of validating ideas through discussions with end users to understand their challenges. It involves conducting 150-200 email interviews, 30-50 phone or video calls, and up to 10 in-person visits. Key tips include focusing on specific customer problems and use cases during interviews, writing down all details from customers rather than summarizing, and talking to each customer individually rather than using forms. The process helps ensure 72% of new products do not fail due to unmet customer expectations.
Why Customer Centricity is your Biggest Competitive AdvantagePRWD
In this presentation PRWD Founder Paul Rouke shared why becoming customer-centric is the biggest competitive advantage for any business. He also shared tips of how to get started using the no.1 technique to start becoming customer-centric.
Paul also shared the Conversion Optimisation Maturity Model which is available as in instant download at bit.ly/CROMaturityModel
Before the Business Plan: Business Modeling Lady Bizness
Do you find the business plan scary and don't know where to start your research? Come learn how using the Lean Canvas Model can help you focus in to the key ingredients to research for your business plan.
Speaker(s): Lady Bizness, Inc.
Scott Stonehocker, Intuit. How to build a culture of customer obsessionIT Arena
Scott is known for building high functioning product teams and driving a culture of customer-centricity. Originally from Canada, he is currently leading product and innovation in Paris France. The teams are constantly looking for the biggest customer pains, then testing and developing solutions to solve them quickly.
Why Customer Centricity is your Biggest Competitive AdvantagePRWD
In this presentation PRWD Founder Paul Rouke shared why becoming customer-centric is the biggest competitive advantage for any business. He also shared tips of how to get started using the no.1 technique to start becoming customer-centric.
Paul also shared the Conversion Optimisation Maturity Model which is available as in instant download at bit.ly/CROMaturityModel
Before the Business Plan: Business Modeling Lady Bizness
Do you find the business plan scary and don't know where to start your research? Come learn how using the Lean Canvas Model can help you focus in to the key ingredients to research for your business plan.
Speaker(s): Lady Bizness, Inc.
Scott Stonehocker, Intuit. How to build a culture of customer obsessionIT Arena
Scott is known for building high functioning product teams and driving a culture of customer-centricity. Originally from Canada, he is currently leading product and innovation in Paris France. The teams are constantly looking for the biggest customer pains, then testing and developing solutions to solve them quickly.
Transforming to a product led company by Josh CentnerProduct Anonymous
Product transformation is easy and everyone in our organisation is both excited and happy to be on the journey….
This sounds far fetched but it is the reality at PageUp. Our speaker, Josh Centner, Head of Product will walk through the journey of PageUp - a successful Melbourne based software company and how they went from a custom software house to a fast paced, customer focused product company.
Josh will cover their approach to introducing organisational transformation and how the organisation combined the best of Design Thinking and Lean Startup to revolutionise the structure, process and mindset that drives strategy, operations and product development practices.
We will hear about structure, culture and process along with what PageUp did with all 3 and what you can take from their experience.
Sales Skills and a little bit more. For many years, I have been successful winning new business, and major accounts.
Here are some really good pointers, some you know, others you may not. Happy Hunting.
Денис Кулик: Customer Discovery: First step for turning an idea into a succes...Provectus
Денис Кулик, Product Manager в компании Readdle в рамках "Product Management: what is it about?"
Organizer Product Management Community - Ukraine
Regional Partner Provectus
Powered by SoftServe
How to Grow and Become a Seasoned PM by Microsoft Sr PMProduct School
Main takeaways:
-Identifying the right stakeholders in your growth
-How to leverage all opportunities correctly with these stakeholders
-Important skills to demonstrate in your work that make you eligible for next level
The Art of Conducting Customer Interviews by Microsoft Prod LeadProduct School
Main Takeaways:
-Understanding types of customer interviews
.Identifying right audience for the interview
-Recognizing untold stories of customer
-To-Dos that makes interview successfu
-Don'ts to avoid during customer interviews
-Simple framework for Customer Interview Lifecycle
How to Thrive, Not Just Survive at Product by BBC PMProduct School
Main Takeaways:
- Know what success looks like and how you intend to measure it upfront
- Identify the key stakeholders and have a plan to manage them
- Be a good storyteller and sell the positive case
Target good enough, not perfect
Creating Actionable Product Strategy by Turo Director of ProductProduct School
Main Takeaways:
- Measure what matters – Establishing the right metrics and KPIs early on can provide tremendous clarity. Driving towards the wrong goals can result in team misalignment, at best, and a failed product strategy, at worst.
- Distinguish the highest impact ideas from the good ideas
- Most companies have lots of good ideas. PMs must separate the great from the good, and craft product strategies that yield the highest impact outcomes for their customers and business.
Iterate, based on customer feedback & data – Great product strategies should evolve over time, with the ongoing incorporation of customer feedback, data, and stakeholder input. Strategies developed in a vacuum are unlikely to succeed, as are strategies that fail to evolve with the changing needs of customers.
I gave a talk on the role of Design Thinking to leaders in the financial industry. The focus was on user centric thinking to innovate financial products and digital services. (all case material is removed)
Transforming to a product led company by Josh CentnerProduct Anonymous
Product transformation is easy and everyone in our organisation is both excited and happy to be on the journey….
This sounds far fetched but it is the reality at PageUp. Our speaker, Josh Centner, Head of Product will walk through the journey of PageUp - a successful Melbourne based software company and how they went from a custom software house to a fast paced, customer focused product company.
Josh will cover their approach to introducing organisational transformation and how the organisation combined the best of Design Thinking and Lean Startup to revolutionise the structure, process and mindset that drives strategy, operations and product development practices.
We will hear about structure, culture and process along with what PageUp did with all 3 and what you can take from their experience.
Sales Skills and a little bit more. For many years, I have been successful winning new business, and major accounts.
Here are some really good pointers, some you know, others you may not. Happy Hunting.
Денис Кулик: Customer Discovery: First step for turning an idea into a succes...Provectus
Денис Кулик, Product Manager в компании Readdle в рамках "Product Management: what is it about?"
Organizer Product Management Community - Ukraine
Regional Partner Provectus
Powered by SoftServe
How to Grow and Become a Seasoned PM by Microsoft Sr PMProduct School
Main takeaways:
-Identifying the right stakeholders in your growth
-How to leverage all opportunities correctly with these stakeholders
-Important skills to demonstrate in your work that make you eligible for next level
The Art of Conducting Customer Interviews by Microsoft Prod LeadProduct School
Main Takeaways:
-Understanding types of customer interviews
.Identifying right audience for the interview
-Recognizing untold stories of customer
-To-Dos that makes interview successfu
-Don'ts to avoid during customer interviews
-Simple framework for Customer Interview Lifecycle
How to Thrive, Not Just Survive at Product by BBC PMProduct School
Main Takeaways:
- Know what success looks like and how you intend to measure it upfront
- Identify the key stakeholders and have a plan to manage them
- Be a good storyteller and sell the positive case
Target good enough, not perfect
Creating Actionable Product Strategy by Turo Director of ProductProduct School
Main Takeaways:
- Measure what matters – Establishing the right metrics and KPIs early on can provide tremendous clarity. Driving towards the wrong goals can result in team misalignment, at best, and a failed product strategy, at worst.
- Distinguish the highest impact ideas from the good ideas
- Most companies have lots of good ideas. PMs must separate the great from the good, and craft product strategies that yield the highest impact outcomes for their customers and business.
Iterate, based on customer feedback & data – Great product strategies should evolve over time, with the ongoing incorporation of customer feedback, data, and stakeholder input. Strategies developed in a vacuum are unlikely to succeed, as are strategies that fail to evolve with the changing needs of customers.
I gave a talk on the role of Design Thinking to leaders in the financial industry. The focus was on user centric thinking to innovate financial products and digital services. (all case material is removed)
In the quest for innovation, companies all over the world are embracing the need for customer insight to drive product development, with many corporates investing in innovation labs with user testing facilities, and embarking on large scale customer research.
These kinds of undertakings do not come cheap - so when you do choose to invest in customer research, how do you make sure you get the most out of your spend?
Here's Wilson Fletcher's view on getting the most out of user research and the best way of implementing it to consistently drive successful innovation.
As presented by Lauren Argenta at Wilson Fletcher's Sydney event on 7th April 2016.
Intro to Lean Startup and Customer Discovery for AgilistsShashi Jain
This is a short presentation I made to the Portland Agile and Scrum group giving a light introduction to Lean Startup, Customer Discovery, and how you use them together to create a product-market fit.
Similar to Kulyk Denus Customer Discovery – Turning an Idea Into a Successful Product (20)
Artem Bykovets: Чому люди не стають раптово кросс-функціональними, хоча в нас...Lviv Startup Club
Artem Bykovets: Чому люди не стають раптово кросс-функціональними, хоча в нас Agile? (UA)
Kyiv PMDay 2024 Summer
Website – www.pmday.org
Youtube – https://www.youtube.com/startuplviv
FB – https://www.facebook.com/pmdayconference
Natalia Renska & Roman Astafiev: Нарциси і психопати в організаціях. Як це вп...Lviv Startup Club
Natalia Renska & Roman Astafiev: Нарциси і психопати в організаціях. Як це впливає на розробку продуктів та реалізацію інноваційних рішень (UA)
Kyiv PMDay 2024 Summer
Website – www.pmday.org
Youtube – https://www.youtube.com/startuplviv
FB – https://www.facebook.com/pmdayconference
Igor Protsenko: Difference between outsourcing and product companies for prod...Lviv Startup Club
Igor Protsenko: Difference between outsourcing and product companies for product managers and related challenges (UA)
Kyiv PMDay 2024 Summer
Website – www.pmday.org
Youtube – https://www.youtube.com/startuplviv
FB – https://www.facebook.com/pmdayconference
Kseniya Leshchenko: Shared development support service model as the way to ma...Lviv Startup Club
Kseniya Leshchenko: Shared development support service model as the way to make small projects with small budgets profitable for the company (UA)
Kyiv PMDay 2024 Summer
Website – www.pmday.org
Youtube – https://www.youtube.com/startuplviv
FB – https://www.facebook.com/pmdayconference
Anna Kompanets: Проблеми впровадження проєктів, про які б ви ніколи не подума...Lviv Startup Club
Anna Kompanets: Проблеми впровадження проєктів, про які б ви ніколи не подумали (UA)
Kyiv PMDay 2024 Summer
Website – www.pmday.org
Youtube – https://www.youtube.com/startuplviv
FB – https://www.facebook.com/pmdayconference
Anton Hlazkov: Впровадження змін – це процес чи проєкт? Чому важливо розуміти...Lviv Startup Club
Anton Hlazkov: Впровадження змін – це процес чи проєкт? Чому важливо розуміти різницю і як це впливає на результат (UA)
Kyiv PMDay 2024 Summer
Website – www.pmday.org
Youtube – https://www.youtube.com/startuplviv
FB – https://www.facebook.com/pmdayconference
Yana Bort: Ритм організації. Чи можливо синхронізувати великий ентерпрайз за ...Lviv Startup Club
Yana Bort: Ритм організації. Чи можливо синхронізувати великий ентерпрайз за допомогою Agile практик? (UA)
Kyiv PMDay 2024 Summer
Website – www.pmday.org
Youtube – https://www.youtube.com/startuplviv
FB – https://www.facebook.com/pmdayconference
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
3. Customer Discovery 101
Customer Discovery is a process of idea validation through dialog with an
end user and discussion of challenges they have with current solutions.
“72% of new products and services innovations fail to
deliver on customer expectations and fail as a result.”
*Source: Simon-Kucher & Partners, 2014
5. Practical Tip #1
FOCUS ON THE SPECIFIC CASE
DURING INTERVIEW WITH A
POTENTIAL CUSTOMER.
Dive deep into specific customer problems. Ask clarifying questions
and try to understand how they work.
6. Practical Tip #2
DO NOT SUMMARIZE
WHAT PEOPLE SAY EVEN AFTER
40 INTERVIEWS
Write down all of the details that customers share in one document.
Outline patterns and use cases in a separate document.
7. Practical Tip #3
DO NOT USE
GOOGLE FORMS.
Do not use forms at all. Talk to each customer individually and do not
treat them like a bunch of contacts that need to fill out the form.