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Overview

Circuit
Result

Modeling

Human
Practice
Three Circuit Types

GFP

1.Original

GFP GFP GFP

2.Positive Feedback
Faster & Higher Intensity

GFP GFP GFP

3.Negative Regulation
More Stable

Overview

Circuit Result

Modeling

Human Practice
1.Original Circuit
Induced
Promoter

RBS

Reporter

Double
Terminator

Constitutive
Promoter

QS
Receptor

GFP

Overview

Circuit Result

Modeling

Human Practice

Double
Terminator
1.Original Circuit
Induced
Promoter

RBS

Reporter

Double
Terminator

Constitutive
Promoter

QS
Receptor

GFP

Overview

Circuit Result

Modeling

Human Practice

Double
Terminator
1.Original Circuit
Induced
Promoter

RBS

Reporter

Double
Terminator

Constitutive
Promoter

QS
Receptor

GFP

Overview

Circuit Result

Modeling

Human Practice

Double
Terminator
1.Original Circuit
Induced
Promoter

RBS

Reporter

Double
Terminator

Constitutive
Promoter

QS
Receptor

GFP

Overview

Circuit Result

Modeling

Human Practice

Double
Terminator
1.Original Circuit
Induced
Promoter

RBS

Reporter

Double
Terminator

Constitutive
Promoter

QS
Receptor

GFP

Overview

Circuit Result

Modeling

Human Practice

Double
Terminator
1.Original Circuit
Induced
Promoter

RBS

Reporter

Double
Terminator

Constitutive
Promoter

QS
Receptor

GFP

Overview

Circuit Result

Modeling

Human Practice

Double
Terminator
1.Original Circuit
Induced
Promoter

RBS

Reporter

Double
Terminator

Constitutive
Promoter

QS
Receptor

GFP

Overview

Circuit Result

Modeling

Human Practice

Double
Terminator
1.Original Circuit
Induced
Promoter

RBS

Reporter

Double
Terminator

Constitutive
Promoter

QS
Receptor

GFP
GFP

Overview

Circuit Result

Modeling

Human Practice

Double
Terminator
ELISA Plate Reader

Overview

Circuit Result

Modeling

Human Practice
ELISA Plate Reader

Overview

Circuit Result

Modeling

Human Practice
Flow Cytometry

Start

Overview

Circuit Result

Modeling

Human Practice
Flow Cytometry

Start

1st Hour

Overview

Circuit Result

Modeling

Human Practice
Flow Cytometry

Start

1st Hour

Overview

2nd Hour

Circuit Result

Modeling

Human Practice
Flow Cytometry

Start

1st Hour

Overview

2nd Hour

Circuit Result

3rd Hour

Modeling

Human Practice
Flow Cytometry

Start

1st Hour

Overview

2nd Hour

Circuit Result

3rd Hour

Modeling

4th Hour

Human Practice
2. Positive Feedback Circuit

GFP

RhIR

pRhI

Overview

Circuit Result

Modeling

Human Practice
2. Positive Feedback Circuit

GFP

RhIR

pRhI

Overview

Circuit Result

Modeling

Human Practice
2. Positive Feedback Circuit

RhIR

GFP

Overview

Circuit Result

RhIR

pRhI

Modeling

Human Practice
2. Positive Feedback Circuit

RhIR

GFP

RhIR

pRhI
RhIR

Overview

Circuit Result

Modeling

Human Practice
2. Positive Feedback Circuit

RhIR

GFP

RhIR

pRhI
RhIR

Overview

Circuit Result

Modeling

Human Practice
2. Positive Feedback Circuit

RhIR

GFP

RhIR

pRhI
RhIR

RhIR

Overview

Circuit Result

Modeling

Human Practice
2. Positive Feedback Circuit

RhIR

GFP

RhIR

pRhI
RhIR

RhIR

Overview

Circuit Result

Modeling

Human Practice
2. Positive Feedback Circuit

RhIR

GFP

RhIR

pRhI
GFP

RhIR

RhIR

Overview

Circuit Result

Modeling

Human Practice
2. Positive Feedback Circuit

RhIR

GFP

RhIR

pRhI
GFP

RhIR

RhIR

RhIR

Overview

Circuit Result

Modeling

Human Practice
2. Positive Feedback Circuit

RhIR

GFP

RhIR

pRhI
GFP

RhIR

RhIR

RhIR

Positive
Feedback
Circuit
Overview

Circuit Result

Modeling

Human Practice
ELISA Plate Reader

Overview

Circuit Result

Modeling

Human Practice
ELISA Plate Reader

Overview

Circuit Result

Modeling

Human Practice
Flow Cytometry

Start

Overview

Circuit Result

Modeling

Human Practice
Flow Cytometry

Start

1st Hour

Overview

Circuit Result

Modeling

Human Practice
Flow Cytometry

Start

1st Hour

Overview

2nd Hour

Circuit Result

Modeling

Human Practice
Flow Cytometry

Start

1st Hour

Overview

2nd Hour

Circuit Result

3rd Hour

Modeling

Human Practice
Flow Cytometry

Start

1st Hour

Overview

2nd Hour

Circuit Result

3rd Hour

Modeling

4th Hour

Human Practice
3. Negative Regulation

Divided into 3 separated steps:

Overview

Circuit Result

Modeling

Human Practice
3. Negative Regulation

Divided into 3 separated steps:
Positive
Feedback

Overview

Circuit Result

Modeling

Human Practice
3. Negative Regulation

Divided into 3 separated steps:
Positive
Feedback

Overview

Repressor

Circuit Result

Modeling

Human Practice
3. Negative Regulation

Divided into 3 separated steps:
Positive
Feedback

Overview

Repressor

Circuit Result

Modeling

Reporter

Human Practice
3. Negative Regulation
Positive
Feedback

Repressor

Reporter
Overview

Circuit Result

Modeling

Human Practice
3. Negative Regulation
Double
Terminator

LasR
pConst

Positive
Feedback

Repressor

Reporter
Overview

Circuit Result

Modeling

Human Practice
3. Negative Regulation
Double
Terminator

LasR
pConst

Positive
Feedback

LasR

Repressor

Reporter
Overview

Circuit Result

Modeling

Human Practice
3. Negative Regulation
Double
Terminator

LasR
pConst
LasR

Positive
Feedback

AHL

Repressor

Reporter
Overview

Circuit Result

Modeling

Human Practice
3. Negative Regulation
Double
Terminator

LasR
pConst
LasR

Positive
Feedback

AHL

LasR

CI

Repressor

pLas

Reporter
Overview

Circuit Result

Modeling

Human Practice
3. Negative Regulation
Double
Terminator

LasR
pConst
LasR

Positive
Feedback

AHL

LasR

CI

Repressor

pLas

Reporter
Overview

Circuit Result

Modeling

Human Practice
3. Negative Regulation
Double
Terminator

LasR
pConst
LasR

Positive
Feedback

AHL

LasR

CI

Repressor

pLas
CI

Reporter
Overview

Circuit Result

Modeling

Human Practice
3. Negative Regulation
Double
Terminator

Positive
Feedback

LasR
pConst
LasR

AHL

LasR

Repressor

CI

pLas
CI

GFP
pCI

Reporter

Luciferase/XFP
Overview

Circuit Result

Modeling

Human Practice
3. Negative Regulation
Double
Terminator

Positive
Feedback

LasR
pConst
LasR

AHL

LasR

Repressor

CI

pLas
CI

GFP
pCI

Reporter

Luciferase/XFP
Overview

Circuit Result

Modeling

Human Practice
3. Negative Regulation
Double
Terminator

Positive
Feedback

LasR
pConst
LasR

AHL

LasR

Repressor

CI

pLas
CI

GFP
pCI

Reporter

Luciferase/XFP
Overview

Circuit Result

Modeling

Human Practice
3. Negative Regulation
Double
Terminator

Positive
Feedback

LasR
pConst
LasR

AHL

LasR

Repressor

CI

pLas
CI

GFP
pCI

Reporter

Luciferase/XFP
Overview

Circuit Result

Modeling

Human Practice
ELISA Plate Reader

Overview

Circuit Result

Modeling

Human Practice
ELISA Plate Reader

Overview

Circuit Result

Modeling

Human Practice
Flow Cytometry

Start

Overview

Circuit Result

Modeling

Human Practice
Flow Cytometry

Start

1st Hour

Overview

Circuit Result

Modeling

Human Practice
Flow Cytometry

Start

1st Hour

Overview

2nd Hour

Circuit Result

Modeling

Human Practice
Flow Cytometry

Start

1st Hour

Overview

2nd Hour

Circuit Result

3rd Hour

Modeling

Human Practice
Flow Cytometry

Start

1st Hour

Overview

2nd Hour

Circuit Result

3rd Hour

Modeling

4th Hour

Human Practice
Comparison Of Three Circuits

GFP

1.Original

GFP GFP GFP

2.Positive Feedback
Faster & Higher Intensity

GFP GFP GFP

3.Negative Regulation
More Stable

Overview

Circuit Result

Modeling

Human Practice
Comparison Of Three Circuits
Normal State
GFP

1.Original

GFP GFP GFP

2.Positive Feedback
Faster & Higher Intensity

GFP GFP GFP

3.Negative Regulation
More Stable

Overview

Circuit Result

Modeling

Human Practice
Comparison Of Three Circuits
QS Signal
GFP

1.Original

GFP GFP GFP

2.Positive Feedback
Faster & Higher Intensity

GFP GFP GFP

3.Negative Regulation
More Stable

Overview

Circuit Result

Modeling

Human Practice

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