This document describes iTRAQ-based proteomics analysis for biomarker discovery. It highlights the advantages of the iTRAQ technique including its high sensitivity and ability to detect post-translational modifications through an untargeted approach. Bioinformatic analysis includes functional annotation, enrichment analysis, clustering, network analysis and statistics. The service offered provides relative protein quantification in complex mixtures using iTRAQ labeling and mass spectrometry that is well-suited for comparing normal, diseased and drug-treated samples.

1. iTRAQ-based
Proteomics Analysis

2. Advantage of our iTRAQ technique:
• Cutting-edge facilities & optimized protocols
• High sensitivity
• Untargeted approach for biomarker discovery
• Post-translational modification is detectable

3. Bioinformatic Analysis:
• Functional annotation and enrichment analysis
• Clustering analysis
• Network analysis
• Statistical analysis

4. • Creative Proteomics offers iTRAQ Labeling protein quantification service
suited for unbiased untargeted biomarker discovery. Relative quantification
of proteins for biomarker discovery in complex mixtures by mass
spectrometry can easily and quickly be achieved using iTRAQ technology.
iTRAQ is ideally suited for comparing normal, diseased, and drug-treated
samples, time course studies, biological replicates and provides relative
quantitation.