Vanessa Ford analyzed an unknown tree bark sample from the Philippines to determine if it had any medicinal or analgesic properties. She boiled and filtered the bark to produce liquid and solid samples. Thin layer chromatography indicated one liquid sample tested positive for an active compound. Melting points of two solid samples were above 150°C, requiring further testing to determine their exact compositions. Future work will involve extracting compounds from the bark and comparing them to known medicinal plants from the Philippines like narra and malunggay trees.

1. Exploring
The
Unknown
Tree Bark Sample
FROM THE PHILIPPINES, AS A NATURAL PRODUCT
Vanessa Ford
Honors Component for SCI 281
Prof. Kimberly Stieglitz
Dec. 15, 2014

2. Objective
Unknown Tree Bark Sample from Philippines
Medicinal or Analgesic Properties?

3. The Aeta People – An indigenous tribe of the Philippines
Background

4. Background
Pterocarpus Indicus Willd,
Narra Tree
Moringa Oleifera
Malunggay Tree
Trees in the Philippines
with known medicinal properties

5. Tea of the
Indigenous Philippine
Tribes
Introduction

6. Experimental Methods
Boiling Bark

7. Concentrating
Sample
Crystallization
Experimental Methods

8. Vacuum Filtering System
Experimental Methods

9. Resulting Products
Experimental Methods
Sample A
(Filtered Liquid)
Sample B
(Filtered Solid)
Sample C
(Filtered Solid,
Re-suspended in water)

10. Fractional Distillation
Experimental Methods
of Sample A (Filtered Liquid)

11. Experimental Methods
Products of Sample A
Distillate Liquid Remaining solution
After distillation
Vacuum filtered Vacuum filtered
Sample D
(liquid)
Sample F
(solid)
Sample E
(liquid)
Sample G
(solid)

12. Experimental Methods
& Results
Thin Layer Chromatography
(TLC)
Liquid Samples (A, C, D, and E)
Sample C (the initial solid, re-suspended in water) was the
only one to test positive for an active compound

13. Melting Point Apparatus
Melting Points of Solid Samples (B, F, and G)
Experimental Methods
& Results
Melting Points of 2 out of 3 solids
were determined to be above 150 °C
Further testing needed in order to
determine exact melting points
Closer to characterization based on
comparison to literature

14. WHOLE PRODUCTS EXTRACTION
Future Direction
V.S

15. Future Direction
Human Digestive System

16. Thank You

17. References
• Shriner, Ralph L., Christine K. F. Hermann, Terence C. Moril, David Y.
Curtin, and Reynold C. Fuson. The Systemic Identification of Organic
Compounds. 8th ed. Hoboken, NJ: Johnson Wiley & Sons, 2004. Print.
• Seager, Spencer L., and Michael R. Slabaugh. Chemistry for Today:
General, Organic, and BioChemistry. 8th ed. Belmont, CA: Mary Finch,
/Cole, Cengage Learning, 2011. Print.
• Seager, Spencer L., and Michael R. Slabaugh. Safety-Scale Laboratory
Experiments for Chemistry for Today. 8th ed. Belmont, CA: Mary Finch,
/Cole, Cengage Learning, 2011. Print.
• Sasidharan, S., Y. Chen, and L. Yoga Latha. "Extraction, Isolation and
Characterization of Bioactive Compounds from Plants' Extracts." African
Journal of Traditional, Complementary, and Alternative Medicines (2010).
NCBI. African Networks on Ethnomedicines. Web. 15 Nov. 2014.
<http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3218439/>.

18. References
• S, Wang, Ding S, Zhang X, and Zhang L. "A New Medicinal Plant
Pterocarpus Indicus." NCBI (1997). NCBI. Web. 15 Nov. 2014.
http://www.ncbi.nlm.nih.gov/pubmed/12572429
• "A Bioactive Polyphenolic Constituent in the Bark of Pterocarpus Indicus,
Willd. I. Isolation and Characterization.” CiNii. Web.
<http://ci.nii.ac.jp/naid/130000025769/>.
• Mbikay, Majamba. "Therapeutic Potential of Moringa Oleifera Leaves in
Chronic Hyperglycemia and Dyslipidemia: A Review." Frontiers in
Pharmacology (2012). Web. 25 Nov. 2014.
• Fahey, Jed W. "Moringa Oleifera: A Review of the Medical Evidence for
Its Nutritional, Therapeutic, and Prophylactic Properties. Part 1." Tree For
Life Journal (2005). Trees for Life Journal. Web. 20 Nov. 2014.
<http://www.tfljournal.org/article.php/20051201124931586>.

19. Media References
• Bark background, http://blog.ppnf.org/vitamin-c-what-it-does-where-
to-get-it-how-to-supplement-it/
• Aeta Family, http://kwekudee-
tripdownmemorylane.blogspot.com/2012/10/aeta-people-one-of-first-
natives-of.html
• Bark pieces, http://www.herbmuseum.ca/content/why-pau-darco-so-
effective
• Cutting of Bark, http://home-remedies.wonderhowto.com/how-
to/make-aspirin-from-willow-tree-0142525/

20. Media References
• Tea in cup,
http://spiritscienceactivenation.net/Oz/phpBB3/viewtopic.php?f=49&t
=182
• Cutting of Bark, http://home-remedies.wonderhowto.com/how-
to/make-aspirin-from-willow-tree-0142525/
• Narra Tree, plants.swtexture.com/2009_01_archive.html
• Moringa Tree, fyi09.wordpress.com/tag/vegetable
• Question Mark, icons.mysiteway.com/legacy-icon/114653-magic-
marker-icon-alhpanumeric-question-mark1-ps/