This document describes 4 natural routes in the Kilkis region of Greece. Route 1 describes Pikrolimni lake, known since ancient times and containing sulfur-rich waters used for healing. Route 2 describes Doirani lake shared with North Macedonia, home to 19 fish species and a unique lake museum. Route 3 describes the Balkan Botanical Garden of Kroussia mountain, open since 1996 with artificial lakes, oak forest, and walking paths. Route 4 describes a historical route along traditional villages built with special architecture, including the picturesque village of Livadia known for its potatoes.