Poster from the I Meeting of Systematics, Biogeography and Evolution Symposia: Phylogenomics and molecular evolution

1. Genetic connectivity patterns of
sympatric Scurria limpets along the
Chilean coast
Lívia Peluso 1,2
and Pablo Saenz Agudelo 1
1
Instituto de Ciencias Ambientales y Evolutivas, Universidad Austral de Chile
2
liviapeluso1@gmail.com

2. ● Sympatric and sister species of Scurria
(Patellogastropoda)
● Habitat: rocky intertidal shores in Peru and Chile
○ S. scurra associated with kelps Lessonia sp.
and Durvillaea antarctica
● Wide geographical distribution: environmental
gradients and biogeographic barrier at 30°
10° S
20° S
30° S
40° S
Map with species geographic distribution (bars).
Colors fallow species names by the shells.
Scurria araucana
Scurria ceciliana
Scurria scurra
Do they share the same genetic
connectivity patterns and barriers to
dispersal?
Introduction

3. Material & methods
SNPs N
S. araucana 76159 57
S. ceciliana 36281 47
S. scurra 88375 68
Paposo
Carrizal Bajo
Temblador
Limarí
Huentelauquén
Puertecillo*
*Only S. scurra
70° W
20°S30°S40°S
● Five/six sites sampled
● Samples preserved in ethanol 100%
● DNA extraction from foot tissue
● RAD-seq (Floragenex)
● Only 1 SNP per locus used; SNPs
filtered for low quality loci and
individuals; 1
1
STACKs and VCFtools
2
R, using packages:: adegenet, LEA, dartR
● Genetic structure: PCA and
cluster analysis 2
● Geographic influence: IBD 2

4. No genetic structure observed
N
Results & Discussion
Scurria ceciliana
Cluster analysis: K=1
Well structured populations
Scurria scurra
Cluster analyses: K=3
Gradual structure w/ geographic
distance
Scurria araucana
Cluster analyses: K=1, but:
K=2K=3

5. Results & Discussion
B. Broitman
& Changolab
C. Asorey
Acknowledgments
¡Gracias!
Geographic distance (km)
PairwiseFst(Nei87)
● S. scurra and S. ceciliana did
not presented isolation by
distance (IBD) patterns
● S. araucana had an IBD pattern,
as expected from the structure
analyses (Mantel statistic r:
0.88; p<0.01):
● The species analyzed had
different and contrasting
genetic connectivity patterns
● Different processes appear to
influence them: S. scurra
connectivity may be limited by
its kelps host dispersal; S.
araucana structure may occur
due to dispersal limitation
and/or association with climate
gradients, S. ceciliana is highly
connected in this region
● Next steps: test the influence of
seascape variables:
temperature, salinity, primary
productivity and currents