CD Bioparticles provides one-stop customization services for gene therapy including nucleic acid synthesis, modifications, encapsulation in delivery systems like nanoparticles and viral vectors, and characterization of physical and biological properties. Their services include design and synthesis of nucleic acids, incorporation of modifications, development of polymer nanoparticles, liposomes, lipid nanoparticles, exosome and viral vectors for delivery, and characterization of size, structure, encapsulation, expression and animal studies. They aim to offer reliable delivery systems and a full suite of services to support academic and industrial customers in gene therapy research and development.

1. CD Bioparticles
Provide one-stop customization service of gene
therapy for academic and industrial customers.

2. Nucleic acid services platform
Gene therapy is developing into precise medicines that can manipulate specific genes in the treatment
of serious diseases and vaccines development. To effectively function in vivo, reliable delivery systems
are very important to the nucleic acids for protecting them from degradation and allowing effective
cellular uptake and release. Based on profound scientific research accumulation, strong R&D strength
and continuous technical support, CD Bioparticles could provide customers with one-stop
customization service as:
Nucleic acid services Delivery systems development Characterization
• Design
• Synthesis
• Modifications
• Polymer-based nanoparticles
• Liposomes
• Cell-based nanoparticles
• Viral Vectors
• Physical characterization
• Cell characterization
• Animal studies
Design
According to the name of the disease or the gene, search the database and literature for the
sequence of the nucleic acid, and select the proper CDS for synthesis.
* Other modifications if needed.
Synthesis
• In Vitro Transcription (IVT)
• Chemical synthesis for siRNA, mRNA, DNA, plasmid, ASO, sgRNA, shRNA
• The corresponding QC testing services
Modifications
5`-Terminal
Cap0/ Cap1/Cap2,
Fluorescent Cap,
IRES/MS2,
UTR modification…
3`-Terminal
Poly(A) synthesis, UTR
modification…
Code Region:
m1ψ, m5C, 2'-OMe,
Pseudo-Uridine, LNA, N6
methyladenine, 5
methylcytidune…

3. Delivery system development platforms
Polymer-based nanoparticles
Due to the wide range of sources and low immunogenicity, cationic polymers, such as PEI, dendrimers,
chitosan, gelatin are widely used nanoscales delivery systems for gene therapy.
With the similar structure of cell membrane, liposomes are considered to be a safer and more
effective delivery system. Its flexibility and robustness in lipid structure, composition, ratio and
preparation methods make liposomes an important artificial carrier for gene therapy. Targeting
ligands, such as peptides, antibodies have been successfully applied in liposomes to achieve
active targeting delivery to specified area. Furthermore, encapsulating fluorescent dyes in liposomes
is very beneficial for later tracking and imaging studies.
Liposomes
www.cd-bioparticles.net
Lipid nanoparticles (LNPs)
• High nucleic acid loading
• Stable in vivo
• Low toxicity
Commercial LNPs
• SM-102/MC3/ALC-0315 for research only
• FDA approved
• Ideal delivery model
Fluorescent liposomes
• Biological tracer
• Visual distinction
LNPs with target groups
• Active targeting delivery
• Tissue/cell selectivity
• High bioavailability

4. Delivery system development platforms
Cell-based nanoparticles
Exosomes are nanovesicles derived from cell membrane with natural targeting ability. CD Bioparticles
provides various exosome production custom services for gene therapy research.
Viral vectors
Viral vectors are a tool commonly used in molecular biology to bring genetic material into cells. The
principle is to use the molecular mechanism of viruses to transmit their genomes into other cells for
infection. Its high effectiveness and tropism make viral vectors one of the most commonly used
carriers for gene therapy. CD Bioparticles could provide a wild range of viral vectors for basic
research and preclinical study.
www.cd-bioparticles.net
Adenoviral vectors (Ads)
• Non-integrating
• Large packaging capacity
• Broad tropism
• Transduce (non-)dividing cells
• High level expression
Lentiviral vectors (LVs)
• Integrating
• Stable expression
• Long-term expression
Retroviral vectors (RVs)
• Integrating
• Transduce dividing cells
Adeno-associated viral vectors (AAVs)
• Non-integrating
• Tissue tropisms
• Various mutants
• Low immunogenicity
• High level and long-term

5. Characterization Platform
• DNA sequencing
• Off-target detection
• Size & PDI
• Morphology
• Zeta potential
• Lipid assay (liposomes only)
• Encapsulation efficiency & Drug loading
Physical characterization
• Cell culture
• Cell targeting
• Gene & protein expression
• Titer definition
• Functional evaluation
• Pseudovirus & plasmids creation (viral vectors only)
• Viral vectors purification (viral vectors only)
Cell characterization
• Animal model establishment
• Immunizations
• Safety evaluation
• Effectiveness evaluation
Animal studies

6. Contact Us
Phone: 1-631-624-4882 (USA) / 44-161-818-6441 (Europe) / Fax:1-631-938-8221
E-mail: info@cd-bioparticles.com / www.cd-bioparticles.net
45-1 Ramsey Road, Shirley, NY 11967, USA