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Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report 1 
1. 
Food – Frenz Report 
Study Visit to New Zealand 
Building a scientific bridge between Europe and New Zealand 
Sara Barrento 
Research Unity of Upgrading Fishery and Aquaculture Products 
National Institute of Biological Resources, Lisboa 
Institute of Biomedical Sciences Abel Salazar 
University of Porto, Portugal 
sarabarrento@yahoo.com 
Ph +351 963 643 696
Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report 
1. State of the art 
Food industry is a fast and growing business that demands a constant knowledge and innovation. In this business sector consumers represent an ever increasing role; knowledge is a global achievement and as a consequence consumers’ expectations and demands are high. In the European Union increased emphasis is being placed on food safety, but also, there is a new concern towards a responsible consumption based on a sustainable and green food production. Considering these challenges, Food Frenz invited EU scientists to visit New Zealand, in order to promote research linkages between EU and New Zealand. New Zealand is a well known country for its food exportations, particularly of seafood, dairy, meat, wine, fruit and vegetables with a well established cooperation between research and industry. Therefore, it offers an important innovative expertise, complementary to that within the EU. 
As a student scientist supported by FoodFrenz, I had the unique opportunity to have a close contact with the food research institutes and industry in New Zealand and, I must say, that the outcome is more than positive. My field of interest focuses on improving the Portuguese live crustacean trade. This is a highly profitable sector especially considering the high demand of live crustaceans that are traditionally kept live in deposit facilities until the culinary preparation as a guarantee of freshness. My research is centered on the biological requirements and the several stressors that affect live crustaceans, in order to optimize the present handling procedures, transportation and storage infrastructures. Considering this field of research and given the several project partners I was hosted by New Zealand Institute for Crop and Food Research (Crop and Food). 
Crop and Food has several research areas and diverse capabilities that cover the "fork to farm" production chain. Our research link and melt point is crustaceans health and post harvest welfare. My main aim was to exchange knowledge in this area, which turned out to be very productive, especially considering that I come from an importer country and New Zealand is a huge exporter, which is an excellent complement. But besides the research area that we have in common and the knowledge exchange that we developed and intend to continue, I had also the opportunity to be introduced to New Zealand research management. 
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Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report 
2. Kia hora! 
The New Zealand Institute for Crop and Food Research (Crop and Food) has its head office at Lincoln (near Christchurch) with regional offices at Auckland, Pukekohe, Hastings, Palmerston North, Nelson, Dunedin, Invermay and Gore as well as a site in Australia at Albury. My formal visit lasted two weeks and I went to the regional office at Auckland, in the North Island, and the regional office at Nelson, in the South Island (Fig. 1). 
Figure 1. New Zealand map showing Crop and Food offices locations. 
During the first week I was welcomed by Dr. Graham Fletcher and his team, responsible for the Processing & Preservation Technologies, in the regional office in Auckland, North Island. There I was introduced to the team researchers and main scientific goals. But, my visit turned out to be much more vast and productive, considering that I did several visits that included not only research facilities but also the seafood industry. These included: the University of Auckland, Moana Pacific Fisheries, Auckland Fish Market and Sanford Limited, all located in Auckland (Fig. 2). 
Figure 2. Moana Pacific Fisheries transportation truck, Auckland Fish Market and Sanford Limited head office signs located at Auckland Fish Market (from left to right). 
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Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report 
During the second week, I was welcomed to Nelson, South Island, by Dr. TC Chadderton and his group team responsible for seafoods and marine extracts. There I had also the opportunity to visit the research facilities of The Cawthron Institute, New Zealand King Salmon and Sealord Shellfish Limited, located in Nelson (Fig. 3). Additionaly, I visited Kiwi Lobster, Fiordland Lobster Co. Ltd in Te Anau and the shore- based depot at Milford Sound. 
Figure 3. Crop and Food facilities in Nelson; Sealord located in Nelson fishing harbour, Kiwi lobster – Fiordland Lobster Co. Ltd. transportation truck at Milford Sound (from left to right). 
This visit was not initially schedule but since I stayed more than the two weeks in New Zealand, Dr. Michael Roberts was very helpful and contacted Dr. Malcom Lawson to arrange me a meeting with Mr. Mike Schuck, the chief executive of Kiwi Lobster, Fiordland Lobster Co. Ltd in Te Anau. 
During the third week I went to Te Anau and visited the facilities of Kiwi Lobster, Fiordland Lobster Co. Ltd settled there. I had also the opportunity to fly from Te Anau to Milford Sound with the floatplane pilot from the company Wings and Water that usually transports lobsters from the Fiords to the live lobster plant in Te Anau. In Milford Sound Mr. Allan Cosgrove, responsible for the Milford Sound shore based depot, welcomed me and showed me the fishing vessels, and explained me how the lobsters are kept live and transported to Te Anau plant. 
Figure 3. Firodlandland map. Airplane sign indicates the location of floatplane landing by the lake Te Anau; to the North is Milford Sound where it is located a fishing vessel harbour and a shore based depot. 
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Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report 
3. Insight into New Zealand seafood research and industry 
Seafood research in New Zealand is deeply involved with the industry; research is centered in the industry needs and it moves towards its demands. To understand this trend it is important to recall that New Zealand faced a severe economical crash in 1987, but in the early 90s several strategies were put in practice and an economical recovery began. This also implicated science, for instance, Crop and Food is one out of nine Crown Research Institutes (CRIs) that were set up in 1992 as government-owned companies. This is a concept that permits research institutes to operate as commercial organizations, borrowing funds form joint ventures and subsidiary companies so that they can fully exploit the commercial potential of new developments for the benefit of New Zealand. In this respect research Institutes have the responsibility to find out applied solutions for real problems to keep on having financial support. Therefore, funding from the government is more difficult to get, and in this matter, Universities have more governmental funding. 
In this instance, research being done at the Institutes is by definition applied; the main goals of Crop and Food group teams visited are to maximize the value of seafood in the marketplace, by studying and understanding postharvest physiology, biochemistry and intrinsic food-related properties of fish tissue. 
Cawthrone Institute, on the other hand, was established in 1919 and is owned by the Cawthron Trust Board. The Trustees approve the strategic direction of the Cawthron Institute, fund a range of science and education focused community initiatives, and appoint the Board of Directors. 
Cawthron’s purpose, resumes to adding value to New Zealand’s environment, business and community through the provision of research based advice, product and services. 
Probably, the best definition of the research that is being done at the New Zealand’s Institutes is Cawthron’s premise: “Research Based Solutions”. 
This also means that the industry is deeply involved and interested in research. There is a natural speculative mind, and therefore a concern to improve and innovate in order to achieve the best results. 
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Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report 
4. Research Institutes 
4.1. Research at the Crop and Food – Auckland 
Dr. Graham Fletcher is the Senior Research Scientist of the Processing and Preservation Technologies group team, mainly focused in the microbiology and seafood quality. The current projects concern: a) high pressure processing mussels (main researcher: Dr. Sravani Gupta with the support and collaboration of Professor Mohammed Farid, Auckland University); b) seafood safety (several collaborations), and c) the isolation, characterization and industrial application of a predatory group of bacteria, the Bdellovibrio, from New Zealand sources. 
Dra. Sravani Gupta is doing a PhD research concerning the utilization of pressure to facilitate the shell opening and to avoid mussels contamination with Vibrionacea and Norovirus. New Zealand is one of the biggest exporters of half shells of green shell mussels. However, opening the shells is a difficult task and prone to contamination, the utilization of pressures besides facilitating the shell opening avoids contaminations. The meat quality and enzyme inactivation is also being investigated; one of the advantages of using this process is the meat yield increase which is quite advantageous for the industry. 
The main seafood problem that New Zealand faces is the green shell mussel contamination with Listeria. A major program is being developed to characterize New Zealand’s strains of Listeria; compare strains in products and environments and genetic basis of biofilm forming and prevalence. This is a major concern and priority due to a recent Listeria outbreak in mussels exported to the USA. Several strategies are being investigated to open the shells (hot water baths, ultraviolet light, utilization of a robot to open the shells and high pressure). 
Bd bacteria is also being investigated from New Zealand sources. Bds are gram-negative prokaryotes isolated from very diverse environments that attack and grow in the periplasm of some other gram-negative bacteria resulting in their lysis. Other Bd strains from different places in the world are also being investigated and successfully applied in the industry: Germany (antibiotic spray), USA (effective killing of E. coli), and in New Zealand it was characterized and isolated for the first time at Crop and Food. At Crop 
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Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report 
and Food, the main objectives are to use these bacterias against seafood bacterias, try to isolate Bd from colder environments and strains able to kill positive and negative bacterias. 
I also visited Auckland University under the supervision of Professor Mohammed Farid, who showed the new technologies being developed at his laboratory: a phase change material that minimizes temperature changes, thus improving for instance cold storage; pulse electric field of high voltage (3000 volts) to inactivate bacteria thus avoiding pasteurization; the utilization of high pressure (9000 atm) to open mussel shells; and a technique to crystallize water, thus removing water and concentrating, for instance, juice. 
4.2. Research at Crop and Food – Nelson 
Dr. TC Chadderton is the manager of the Seafood & marine extracts group. The group develops and delivers harvesting, handling, processing and preservation technologies for New Zealand’s fishing, aquaculture and seafood processing sectors. 
Visiting Nelson was very fruitful for my investigation; I did an oral presentation to introduce the research unity where I work in portugal and the investigation on post harvest stress of edible crab (Cancer pagurus). This area is similar to the work being developed by Doctor Alister Jerret and Dr. Michael Roberts. The meeting conversation that we had was very productive, Dr. Michael Roberts measured several stress parameter in the rock lobster fisheries (pH, glucose, lactate), inorganic ions (K, Mg, Ca, Cl and Na), and also the vigor. Considering that I am going to start stress parameters measurements, this meeting was very enlightening. Also, New Zealand’s industry is starting to exploit a deep red spider crab that has never been exported, and the transportation conditions have not been investigated yet. This was also a starting point to establish future contacts. 
Moreover, this group has strong support from the seafood industry and is deeply committed in taking new discoveries to the market. In this respect, Dr. Jan Holland provided some information about AquiSTM, which is one of the examples of a successful product developed by Crop and Food that is presently being commercialized. AquiSTM is a fish anaesthetic/sedative commercialized in many countries to be used as a fish aquaculture sedative. Few studies have been developed in crustaceans, but preliminary studies indicate that crabs are susceptible to be easily sedated by AquiSTM. However, there is the indication that a sweeter 
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Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report 
taste than normal might be perceived in the seafood, especially in crustaceans. This might be due to a less stressed physiological condition that prevents the production of metabolites, such as lactic acid. 
This product might be useful in the transportation of live edible crabs, which is a critical step in the trade of these animals. In Portugal, the main problem is the high mortalities during transportation and 48 h after transportation which can reach up to 25 %. At the Research Unity of Upgrading Fishery and Aquaculture Products, in Portugal, there is the interest to test this product. We have contacted by email Dr. Gunnar Molland and we were given a free sample that we just tested in the end of May. In this experiment we compared the stress effects of animals treated with AquiSTM vs no AquiSTM in simulated transportation conditions at three different temperatures (8, 12 and 15 ºC). During the experiments I periodically analyzed the animal vigor, collected haemolymph samples and seawater samples. At the end of the experiment, sensorial analysis of animals (raw and cooked animals) was analyzed. The results are being studied and will be disseminated as soon as possible. 
4.3. Research at Cawthron 
At Cawthron Institute there was an interview meeting with Dr. Norman Ragg a shellfish physiologist working in the aquaculture group, Dr. Leslie Rhodes senior research scientist working with the Biosecurity and Biotechnology group and Aditya Kesarcodi-Watson, aquaculture scientist. 
The aquaculture group works with the industry to advance New Zealand’s marine farming, from breeding to harvest, main projects are focused in greenshell mussel and oysters production, probiotics development and application. 
The Biosecurity and Biotechnology Group focuses on research and advice for the biosecurity of aquatic ecosystems, seafood safety and biotechnology. Our discussion focused on the production of biotoxins and the up take of palytoxin by paddle crabs (Ovalipes catharus White 1843). The uptake of biotoxins by crustaceans is an important issue that has not been much investigated. In Europe the main concern is towards bivalves, and few studies have been developed regarding crustaceans. This is issue can be important in the near future and should be address. 
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Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report 
5. Seafood Industry 
5.1 Moana Pacific Fisheries - Auckland 
In Auckland a visit was arranged to the Moana Pacific Fisheries live crustaceans’ plant infrastructures, where permission was given to see the stocking tanks, the package room and the biofilters. I was very welcomed and allowed to take pictures. 
Moana Pacific Fisheries selective harvests premium white flesh fish species predominantly by long line catching. Rock lobster is one of the twenty one species that are harvested and is caught by potting. The fishery is open year round, however the highest catches tend to occur from October to December. Most New Zealand Rock Lobster is delivered live to shore-based plants either for conditioning for live export packs or processed into frozen tails, or frozen whole fish. 
The Lobster fishery in New Zealand is strictly controlled the minimum size is set at 54 mm tail width for males and 60 mm tail width for females. 
The plants’ closed system has several storage tanks with at least six biofilters (coral) as high as 2 m (Fig. 4), the total tanks capacity is of 12 tons of lobsters. There is a real concern about water quality and management, water tests are routinely done (pH, salinity, ammonia, nitrates, nitrites and alkalinity) and measures are put into action if the water equilibrium is disturbed, for instance, if pH is too low, soda ash is added. The mortalities at the plant are low, less than 10 %. 
At arrival to the plant lobsters are graded, according to size, 8 – 12 kg per poly box; grade weight: 
A 0 - 600gms; 
B 
A 
B 600 - 800gms; 
C 800 - 1000gms; 
D 1,000 - 1,500gms; 
E 1,500 - 2,500gms; 
F 2,500gms+. 
Figure 4. A) Packaging room; B) biofilters filled with coral in a cylindrical 2 m container. 
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Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report 
If lobsters are missing more than three legs they are not exported. The chambers are kept at 12 º C but the day previous to the exportation, lobsters are chilled to 7 º C. Lobster are stocked for a maximum of 5 days, depending on the market demand. The main markets are Asia (Hong Kong and Japan); prices depend on lobster size (smaller lobster are more expensive in Asia but less expensive in the USA), June, July and February (Chinese new year’s) are the best selling months. 
5.2. New Zealand King Salmon - Nelson 
The New Zealand King Salmon Co Ltd is a well established group in the world’s commercial salmon farming industry. A visit was arranged to the company Head Office in Nelson and to a processing facility where it was possible to see the manual filleting, the cooler and smoking chambers. The company currently operates five seacage sites, all located in the remote, clean waters of the Marlborough Sounds. The company also owns and runs a research breeding facility, two hatcheries for smolt rearing and 4 purpose built processing facilities in Nelson. 
The company harvests approximately 6000mt (metric tonne) per annum which can be considered a small producer by world standards where production is over 1.5 million tones. Nevertheless it is a fast growing business where New Zealand market takes around one half of production. Japan is the largest export market taking 21% of production. A further 15% goes to Australia with the balance going to South East Asia and the USA. Salmon is processed for delivery in a variety of forms including fresh, frozen, smoked, marinaded and sashimi. 
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Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report 
5.4. Sealord Shellfish Limited - Nelson 
A meeting visit was arranged to Sealord offices in Nelson fishing harbour and to the Greenshell™ Mussel processing plant, the largest Greenshell™ Mussel processing plant in the world (18,000 greenweight tonnes a year). We were allowed to see the seventy workers opening the shells. During the low season (June to September) only one shift is working - seventy two workers opening shells in a 7.5 h day while during the rest of the year, 144 people work in two shifts. The shells are supplied to a cement factory. 
Sealord is home based in New Zealand having secure access to about 19 percent (about 113,000 green weight tonnes) of the total fishing quota in the New Zealand 200 mile Exclusive Economic Zone; but it has also several other bases in about 15 countries. The company sells more than 1500 products to customers world-wide. 
5.5. Kiwi lobster – Fiordland Lobster Co. Ltd. 
In Te Anau Mr. Mike Schuck, the chief executive of Kiwi Lobster, Fiordland Lobster Co. Ltd kindly showed the plant and explained the management of the Rock lobster and how live exportation started in first place in Fiordland. 
The Fiordland Lobster Company is the outcome of a partnership formed between fifteen Fiordland fishermen and Mt. Maunganui Seafoods (a North island seafood processor) in 1989. It is interesting to note that before this time lobsters were tailed, frozen and exported to the USA market. The lobster bodies were discarded as waste. The live lobster export is therefore a recent achievement that saw the first exports of Fiordland live lobster into Japan in 1988. The implementation of the new lobster measure and Quota Management System in 1986, turned out to be a driving force to establish a successful business. 
The Quota Management System is based in an Individual Transferable Quota (ITQ) that gives the right to harvest a defined amount of a species (percentage by weight of the TACC, total allowable commercial catch) in a specified area during one fishing year. When a species is first brought under the Quota Management System, Maori are given 20% of the total quota. The remainder is distributed amongst those 
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Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report 
people who hold commercial fishing permits for that species - based on how much they caught over previous years. The quota rights can be transferred to another person or company through either sale or lease. For most quota-managed species the year runs from 1 October to 30 September. A monitoring process is set in place to ensure that actual landed catches are always matched against someone's quota. The Fisheries Act limits how much quota any one person or company can own - so that that no one company or individual can develop a monopoly on fishing in any one area or for any one species. The leading New Zealand fishing companies and quota holders are currently Sealord, Sanford, and Talley's. 
At Fiordland fishermen catch lobsters in a depth from 5 m up to 200 m by potting, each boat can have up to 250 pots being operated by two or three man (Fig. 5). They can catch up to 2 tons of lobsters per day, and since the Asian market price can change 5$/day, there is an investment in holding the product in an attempt to get the best price. They can hold 5 to 6 tons of lobsters in seacages (each having 300 – 400 Kg) in the Fiords. However, water physicochemical properties change rapidly in the Fiords due to spring water input from rain and melting ice, water surface has therefore, a quite low salinity compared to deeper water. Temperature is also different, especially in the winter, surface temperature can be 19 º C but deep water is only at 12 º C. These water variations and octopus predation are a drawback to holding lobsters in the Fiords. 
Figure 5. Fishing vessel and pots at Milford Sound; depot tanks at Te Anau factory(from left to right) 
The Fiordland Lobster Company contract fishermen suppliers to land their lobster catch at shore based depots spread around the country or have it flown via helicopter or floatplane directly to one of the pack factories like the plant factory visited in Te Anau (Fig 6). The Milford Sound shore based depot can hold up to 2.5 tons while the Te Anau can hold 10 to 14 tons. Once received the lobsters are weighted, graded and 
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Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report 
then swum in the international tanks before export. A minimum swimming time of 24 hours- 48 hours is expected before the lobster is packed in wood wool and ice for transport to either Auckland or Christchurch International Airport. Major markets are China (95 %), Japan (3.9 %) and Dubai (1.1 %), occasionally Italy. Shipments to Asia have mortalities lower than 5 %, in fact customers can only reclaim when mortalities are higher than 5 %. The flight to China lasts up to 35 – 40 h and in fact it has been established that lobster can sustain up to 72 h. 
Figure 6. Fiordland Lobster Company Operation Map. The blue CRA areas are the rock lobster permit areas in which this company has interest in. 
6. Conclusions and future perspectives 
The study visit to New Zealand was very enlightening as far as research and industry management is concerned. The strong applied research with the financial support of the industry promotes a more competitive and innovative market. It is important to highlight that during my visit I had the unique opportunity to visit 5 prestigious companies. All of them were very interested and helpful. Moreover, I had access to Crop and Food Library, where I could gather much useful information, not only related to research but also to the codes of practice of the Seafood industry training organization. This will be very helpful, considering IPIMAR is currently developing a training program to the Portuguese live crustacean’s traders. Many ideas related to my research area have also taken form and at least a preliminary experiment to test AquiSTM has already been done at the Research Unity of Upgrading Fishery and Aquaculture Products facilities. This preliminary experiment is going to be complemented and scientific communication will take place in short term. 
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Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report 
As far as live crustaceans trade is concerned comparing to Portugal, New Zealand has several advantages that are related to the fact that it is an exporter country specialized in an expensive crustacean, the Rock lobster. Moreover, New Zealand is geographically located near important Asian markets, especially Hong Kong and Japan, a market willing to pay for a supreme quality live lobster. Besides the market advantages, there are technical issues that facilitate the live export: captivity duration is low and water management is specific for one species only. Also, New Zealand has closed depots with a very strict water control. 
In Portugal, bigger import depots are usually open or semi open systems with a poor water quality management. Some facilities do not cool the water and at least nine species are traded live: four species of crabs, Cancer. pagurus, Maja spp., Necora. puber and Carcinus maenas; two species of clawed lobsters, Homarus gammarus, H. americanus, and at least three species of spiny lobsters Palinurus elephas, P. mauritanicus, and Panulirus regius. The trade of so many different species, with different habitats and physiological needs is a major drawback to settle plant facilities technically suited to keep all these crustaceans live. Also, captivity can be as short as one day and as long as three months depending on species. Though Portugal has a long lasting tradition of keeping live crustaceans in depots, water management is very deficient and attitudes are difficult to change especially considering that this is usually a family business that in some companies dates back from the beginning of the 20th century. 
In New Zealand this is a recent business where there was a big investment in training; this is just starting in Portugal. Also, since it is an export country the need to settle in the international trade promotes innovation and the search for the necessary improvements to face the market demands. 
Needless to say that New Zealand has pristine waters where the cool environment and unspoiled coastline provides a fishery haven abundant with high quality seafood. In addition to these unique conditions, fisheries are efficiently managed; access to New Zealand's fishing resources is protected by a 1986 Act of Parliament. The sophisticated Quota Management System controls the harvesting of the natural fish resources to sustain the resources for future generations and is actively supported by the New Zealand fishing industry. 
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Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report 
Bibliography 
a) Cawthron annual report, 2007 
b) Crop & Food Research, Infosheet No. 2 – 21 2006 
c) FAO/WHO (2001) Health and Nutritional Properties of Probiotics in Food including Powder Milk with Live Lactic Acid Bacteria. Report of a Joint FAO/WHO Expert Consultation on Evaluation of Health and Nutritional Properties of Probiotics in Food Including Powder Milk with Live Lactic Acid Bacteria. 
Sites in the internet: 
http://www.lobster.co.nz/the_process.html 
http://www.seafood.co.nz/qms 
http://www.sealord.co.nz/xml/default.asp?globalPage=C_environment 
http://www.moanapacific.com/index1.htm 
http://www.kingsalmon.co.nz/Company/ 
Acknowledgments 
I must acknowledge FoodFrenz, all Crop and Food researchers who shared their research and accompanied me to the several companies visited and all managers and technicians of these companies. 
I must make a special acknowledgement to Dr. Michael Roberts and Doctor Alister Jerret that put me in contact with Dr. Malcom Lawson. Also, a special thanks to Mr. Mike Schuck who kindly received me at Te Anau and Mr. Allan Cosgrove who took part of his Sunday afternoon to meet me at Milford Sound harbour. 
This visit truly beneficiated my investigation back in Portugal. 
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Building a Scientific Bridge between Europe and New Zealand

  • 1. Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report 1 1. Food – Frenz Report Study Visit to New Zealand Building a scientific bridge between Europe and New Zealand Sara Barrento Research Unity of Upgrading Fishery and Aquaculture Products National Institute of Biological Resources, Lisboa Institute of Biomedical Sciences Abel Salazar University of Porto, Portugal sarabarrento@yahoo.com Ph +351 963 643 696
  • 2. Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report 1. State of the art Food industry is a fast and growing business that demands a constant knowledge and innovation. In this business sector consumers represent an ever increasing role; knowledge is a global achievement and as a consequence consumers’ expectations and demands are high. In the European Union increased emphasis is being placed on food safety, but also, there is a new concern towards a responsible consumption based on a sustainable and green food production. Considering these challenges, Food Frenz invited EU scientists to visit New Zealand, in order to promote research linkages between EU and New Zealand. New Zealand is a well known country for its food exportations, particularly of seafood, dairy, meat, wine, fruit and vegetables with a well established cooperation between research and industry. Therefore, it offers an important innovative expertise, complementary to that within the EU. As a student scientist supported by FoodFrenz, I had the unique opportunity to have a close contact with the food research institutes and industry in New Zealand and, I must say, that the outcome is more than positive. My field of interest focuses on improving the Portuguese live crustacean trade. This is a highly profitable sector especially considering the high demand of live crustaceans that are traditionally kept live in deposit facilities until the culinary preparation as a guarantee of freshness. My research is centered on the biological requirements and the several stressors that affect live crustaceans, in order to optimize the present handling procedures, transportation and storage infrastructures. Considering this field of research and given the several project partners I was hosted by New Zealand Institute for Crop and Food Research (Crop and Food). Crop and Food has several research areas and diverse capabilities that cover the "fork to farm" production chain. Our research link and melt point is crustaceans health and post harvest welfare. My main aim was to exchange knowledge in this area, which turned out to be very productive, especially considering that I come from an importer country and New Zealand is a huge exporter, which is an excellent complement. But besides the research area that we have in common and the knowledge exchange that we developed and intend to continue, I had also the opportunity to be introduced to New Zealand research management. 2
  • 3. Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report 2. Kia hora! The New Zealand Institute for Crop and Food Research (Crop and Food) has its head office at Lincoln (near Christchurch) with regional offices at Auckland, Pukekohe, Hastings, Palmerston North, Nelson, Dunedin, Invermay and Gore as well as a site in Australia at Albury. My formal visit lasted two weeks and I went to the regional office at Auckland, in the North Island, and the regional office at Nelson, in the South Island (Fig. 1). Figure 1. New Zealand map showing Crop and Food offices locations. During the first week I was welcomed by Dr. Graham Fletcher and his team, responsible for the Processing & Preservation Technologies, in the regional office in Auckland, North Island. There I was introduced to the team researchers and main scientific goals. But, my visit turned out to be much more vast and productive, considering that I did several visits that included not only research facilities but also the seafood industry. These included: the University of Auckland, Moana Pacific Fisheries, Auckland Fish Market and Sanford Limited, all located in Auckland (Fig. 2). Figure 2. Moana Pacific Fisheries transportation truck, Auckland Fish Market and Sanford Limited head office signs located at Auckland Fish Market (from left to right). 3
  • 4. Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report During the second week, I was welcomed to Nelson, South Island, by Dr. TC Chadderton and his group team responsible for seafoods and marine extracts. There I had also the opportunity to visit the research facilities of The Cawthron Institute, New Zealand King Salmon and Sealord Shellfish Limited, located in Nelson (Fig. 3). Additionaly, I visited Kiwi Lobster, Fiordland Lobster Co. Ltd in Te Anau and the shore- based depot at Milford Sound. Figure 3. Crop and Food facilities in Nelson; Sealord located in Nelson fishing harbour, Kiwi lobster – Fiordland Lobster Co. Ltd. transportation truck at Milford Sound (from left to right). This visit was not initially schedule but since I stayed more than the two weeks in New Zealand, Dr. Michael Roberts was very helpful and contacted Dr. Malcom Lawson to arrange me a meeting with Mr. Mike Schuck, the chief executive of Kiwi Lobster, Fiordland Lobster Co. Ltd in Te Anau. During the third week I went to Te Anau and visited the facilities of Kiwi Lobster, Fiordland Lobster Co. Ltd settled there. I had also the opportunity to fly from Te Anau to Milford Sound with the floatplane pilot from the company Wings and Water that usually transports lobsters from the Fiords to the live lobster plant in Te Anau. In Milford Sound Mr. Allan Cosgrove, responsible for the Milford Sound shore based depot, welcomed me and showed me the fishing vessels, and explained me how the lobsters are kept live and transported to Te Anau plant. Figure 3. Firodlandland map. Airplane sign indicates the location of floatplane landing by the lake Te Anau; to the North is Milford Sound where it is located a fishing vessel harbour and a shore based depot. 4
  • 5. Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report 3. Insight into New Zealand seafood research and industry Seafood research in New Zealand is deeply involved with the industry; research is centered in the industry needs and it moves towards its demands. To understand this trend it is important to recall that New Zealand faced a severe economical crash in 1987, but in the early 90s several strategies were put in practice and an economical recovery began. This also implicated science, for instance, Crop and Food is one out of nine Crown Research Institutes (CRIs) that were set up in 1992 as government-owned companies. This is a concept that permits research institutes to operate as commercial organizations, borrowing funds form joint ventures and subsidiary companies so that they can fully exploit the commercial potential of new developments for the benefit of New Zealand. In this respect research Institutes have the responsibility to find out applied solutions for real problems to keep on having financial support. Therefore, funding from the government is more difficult to get, and in this matter, Universities have more governmental funding. In this instance, research being done at the Institutes is by definition applied; the main goals of Crop and Food group teams visited are to maximize the value of seafood in the marketplace, by studying and understanding postharvest physiology, biochemistry and intrinsic food-related properties of fish tissue. Cawthrone Institute, on the other hand, was established in 1919 and is owned by the Cawthron Trust Board. The Trustees approve the strategic direction of the Cawthron Institute, fund a range of science and education focused community initiatives, and appoint the Board of Directors. Cawthron’s purpose, resumes to adding value to New Zealand’s environment, business and community through the provision of research based advice, product and services. Probably, the best definition of the research that is being done at the New Zealand’s Institutes is Cawthron’s premise: “Research Based Solutions”. This also means that the industry is deeply involved and interested in research. There is a natural speculative mind, and therefore a concern to improve and innovate in order to achieve the best results. 5
  • 6. Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report 4. Research Institutes 4.1. Research at the Crop and Food – Auckland Dr. Graham Fletcher is the Senior Research Scientist of the Processing and Preservation Technologies group team, mainly focused in the microbiology and seafood quality. The current projects concern: a) high pressure processing mussels (main researcher: Dr. Sravani Gupta with the support and collaboration of Professor Mohammed Farid, Auckland University); b) seafood safety (several collaborations), and c) the isolation, characterization and industrial application of a predatory group of bacteria, the Bdellovibrio, from New Zealand sources. Dra. Sravani Gupta is doing a PhD research concerning the utilization of pressure to facilitate the shell opening and to avoid mussels contamination with Vibrionacea and Norovirus. New Zealand is one of the biggest exporters of half shells of green shell mussels. However, opening the shells is a difficult task and prone to contamination, the utilization of pressures besides facilitating the shell opening avoids contaminations. The meat quality and enzyme inactivation is also being investigated; one of the advantages of using this process is the meat yield increase which is quite advantageous for the industry. The main seafood problem that New Zealand faces is the green shell mussel contamination with Listeria. A major program is being developed to characterize New Zealand’s strains of Listeria; compare strains in products and environments and genetic basis of biofilm forming and prevalence. This is a major concern and priority due to a recent Listeria outbreak in mussels exported to the USA. Several strategies are being investigated to open the shells (hot water baths, ultraviolet light, utilization of a robot to open the shells and high pressure). Bd bacteria is also being investigated from New Zealand sources. Bds are gram-negative prokaryotes isolated from very diverse environments that attack and grow in the periplasm of some other gram-negative bacteria resulting in their lysis. Other Bd strains from different places in the world are also being investigated and successfully applied in the industry: Germany (antibiotic spray), USA (effective killing of E. coli), and in New Zealand it was characterized and isolated for the first time at Crop and Food. At Crop 6
  • 7. Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report and Food, the main objectives are to use these bacterias against seafood bacterias, try to isolate Bd from colder environments and strains able to kill positive and negative bacterias. I also visited Auckland University under the supervision of Professor Mohammed Farid, who showed the new technologies being developed at his laboratory: a phase change material that minimizes temperature changes, thus improving for instance cold storage; pulse electric field of high voltage (3000 volts) to inactivate bacteria thus avoiding pasteurization; the utilization of high pressure (9000 atm) to open mussel shells; and a technique to crystallize water, thus removing water and concentrating, for instance, juice. 4.2. Research at Crop and Food – Nelson Dr. TC Chadderton is the manager of the Seafood & marine extracts group. The group develops and delivers harvesting, handling, processing and preservation technologies for New Zealand’s fishing, aquaculture and seafood processing sectors. Visiting Nelson was very fruitful for my investigation; I did an oral presentation to introduce the research unity where I work in portugal and the investigation on post harvest stress of edible crab (Cancer pagurus). This area is similar to the work being developed by Doctor Alister Jerret and Dr. Michael Roberts. The meeting conversation that we had was very productive, Dr. Michael Roberts measured several stress parameter in the rock lobster fisheries (pH, glucose, lactate), inorganic ions (K, Mg, Ca, Cl and Na), and also the vigor. Considering that I am going to start stress parameters measurements, this meeting was very enlightening. Also, New Zealand’s industry is starting to exploit a deep red spider crab that has never been exported, and the transportation conditions have not been investigated yet. This was also a starting point to establish future contacts. Moreover, this group has strong support from the seafood industry and is deeply committed in taking new discoveries to the market. In this respect, Dr. Jan Holland provided some information about AquiSTM, which is one of the examples of a successful product developed by Crop and Food that is presently being commercialized. AquiSTM is a fish anaesthetic/sedative commercialized in many countries to be used as a fish aquaculture sedative. Few studies have been developed in crustaceans, but preliminary studies indicate that crabs are susceptible to be easily sedated by AquiSTM. However, there is the indication that a sweeter 7
  • 8. Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report taste than normal might be perceived in the seafood, especially in crustaceans. This might be due to a less stressed physiological condition that prevents the production of metabolites, such as lactic acid. This product might be useful in the transportation of live edible crabs, which is a critical step in the trade of these animals. In Portugal, the main problem is the high mortalities during transportation and 48 h after transportation which can reach up to 25 %. At the Research Unity of Upgrading Fishery and Aquaculture Products, in Portugal, there is the interest to test this product. We have contacted by email Dr. Gunnar Molland and we were given a free sample that we just tested in the end of May. In this experiment we compared the stress effects of animals treated with AquiSTM vs no AquiSTM in simulated transportation conditions at three different temperatures (8, 12 and 15 ºC). During the experiments I periodically analyzed the animal vigor, collected haemolymph samples and seawater samples. At the end of the experiment, sensorial analysis of animals (raw and cooked animals) was analyzed. The results are being studied and will be disseminated as soon as possible. 4.3. Research at Cawthron At Cawthron Institute there was an interview meeting with Dr. Norman Ragg a shellfish physiologist working in the aquaculture group, Dr. Leslie Rhodes senior research scientist working with the Biosecurity and Biotechnology group and Aditya Kesarcodi-Watson, aquaculture scientist. The aquaculture group works with the industry to advance New Zealand’s marine farming, from breeding to harvest, main projects are focused in greenshell mussel and oysters production, probiotics development and application. The Biosecurity and Biotechnology Group focuses on research and advice for the biosecurity of aquatic ecosystems, seafood safety and biotechnology. Our discussion focused on the production of biotoxins and the up take of palytoxin by paddle crabs (Ovalipes catharus White 1843). The uptake of biotoxins by crustaceans is an important issue that has not been much investigated. In Europe the main concern is towards bivalves, and few studies have been developed regarding crustaceans. This is issue can be important in the near future and should be address. 8
  • 9. Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report 5. Seafood Industry 5.1 Moana Pacific Fisheries - Auckland In Auckland a visit was arranged to the Moana Pacific Fisheries live crustaceans’ plant infrastructures, where permission was given to see the stocking tanks, the package room and the biofilters. I was very welcomed and allowed to take pictures. Moana Pacific Fisheries selective harvests premium white flesh fish species predominantly by long line catching. Rock lobster is one of the twenty one species that are harvested and is caught by potting. The fishery is open year round, however the highest catches tend to occur from October to December. Most New Zealand Rock Lobster is delivered live to shore-based plants either for conditioning for live export packs or processed into frozen tails, or frozen whole fish. The Lobster fishery in New Zealand is strictly controlled the minimum size is set at 54 mm tail width for males and 60 mm tail width for females. The plants’ closed system has several storage tanks with at least six biofilters (coral) as high as 2 m (Fig. 4), the total tanks capacity is of 12 tons of lobsters. There is a real concern about water quality and management, water tests are routinely done (pH, salinity, ammonia, nitrates, nitrites and alkalinity) and measures are put into action if the water equilibrium is disturbed, for instance, if pH is too low, soda ash is added. The mortalities at the plant are low, less than 10 %. At arrival to the plant lobsters are graded, according to size, 8 – 12 kg per poly box; grade weight: A 0 - 600gms; B A B 600 - 800gms; C 800 - 1000gms; D 1,000 - 1,500gms; E 1,500 - 2,500gms; F 2,500gms+. Figure 4. A) Packaging room; B) biofilters filled with coral in a cylindrical 2 m container. 9
  • 10. Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report If lobsters are missing more than three legs they are not exported. The chambers are kept at 12 º C but the day previous to the exportation, lobsters are chilled to 7 º C. Lobster are stocked for a maximum of 5 days, depending on the market demand. The main markets are Asia (Hong Kong and Japan); prices depend on lobster size (smaller lobster are more expensive in Asia but less expensive in the USA), June, July and February (Chinese new year’s) are the best selling months. 5.2. New Zealand King Salmon - Nelson The New Zealand King Salmon Co Ltd is a well established group in the world’s commercial salmon farming industry. A visit was arranged to the company Head Office in Nelson and to a processing facility where it was possible to see the manual filleting, the cooler and smoking chambers. The company currently operates five seacage sites, all located in the remote, clean waters of the Marlborough Sounds. The company also owns and runs a research breeding facility, two hatcheries for smolt rearing and 4 purpose built processing facilities in Nelson. The company harvests approximately 6000mt (metric tonne) per annum which can be considered a small producer by world standards where production is over 1.5 million tones. Nevertheless it is a fast growing business where New Zealand market takes around one half of production. Japan is the largest export market taking 21% of production. A further 15% goes to Australia with the balance going to South East Asia and the USA. Salmon is processed for delivery in a variety of forms including fresh, frozen, smoked, marinaded and sashimi. 10
  • 11. Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report 5.4. Sealord Shellfish Limited - Nelson A meeting visit was arranged to Sealord offices in Nelson fishing harbour and to the Greenshell™ Mussel processing plant, the largest Greenshell™ Mussel processing plant in the world (18,000 greenweight tonnes a year). We were allowed to see the seventy workers opening the shells. During the low season (June to September) only one shift is working - seventy two workers opening shells in a 7.5 h day while during the rest of the year, 144 people work in two shifts. The shells are supplied to a cement factory. Sealord is home based in New Zealand having secure access to about 19 percent (about 113,000 green weight tonnes) of the total fishing quota in the New Zealand 200 mile Exclusive Economic Zone; but it has also several other bases in about 15 countries. The company sells more than 1500 products to customers world-wide. 5.5. Kiwi lobster – Fiordland Lobster Co. Ltd. In Te Anau Mr. Mike Schuck, the chief executive of Kiwi Lobster, Fiordland Lobster Co. Ltd kindly showed the plant and explained the management of the Rock lobster and how live exportation started in first place in Fiordland. The Fiordland Lobster Company is the outcome of a partnership formed between fifteen Fiordland fishermen and Mt. Maunganui Seafoods (a North island seafood processor) in 1989. It is interesting to note that before this time lobsters were tailed, frozen and exported to the USA market. The lobster bodies were discarded as waste. The live lobster export is therefore a recent achievement that saw the first exports of Fiordland live lobster into Japan in 1988. The implementation of the new lobster measure and Quota Management System in 1986, turned out to be a driving force to establish a successful business. The Quota Management System is based in an Individual Transferable Quota (ITQ) that gives the right to harvest a defined amount of a species (percentage by weight of the TACC, total allowable commercial catch) in a specified area during one fishing year. When a species is first brought under the Quota Management System, Maori are given 20% of the total quota. The remainder is distributed amongst those 11
  • 12. Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report people who hold commercial fishing permits for that species - based on how much they caught over previous years. The quota rights can be transferred to another person or company through either sale or lease. For most quota-managed species the year runs from 1 October to 30 September. A monitoring process is set in place to ensure that actual landed catches are always matched against someone's quota. The Fisheries Act limits how much quota any one person or company can own - so that that no one company or individual can develop a monopoly on fishing in any one area or for any one species. The leading New Zealand fishing companies and quota holders are currently Sealord, Sanford, and Talley's. At Fiordland fishermen catch lobsters in a depth from 5 m up to 200 m by potting, each boat can have up to 250 pots being operated by two or three man (Fig. 5). They can catch up to 2 tons of lobsters per day, and since the Asian market price can change 5$/day, there is an investment in holding the product in an attempt to get the best price. They can hold 5 to 6 tons of lobsters in seacages (each having 300 – 400 Kg) in the Fiords. However, water physicochemical properties change rapidly in the Fiords due to spring water input from rain and melting ice, water surface has therefore, a quite low salinity compared to deeper water. Temperature is also different, especially in the winter, surface temperature can be 19 º C but deep water is only at 12 º C. These water variations and octopus predation are a drawback to holding lobsters in the Fiords. Figure 5. Fishing vessel and pots at Milford Sound; depot tanks at Te Anau factory(from left to right) The Fiordland Lobster Company contract fishermen suppliers to land their lobster catch at shore based depots spread around the country or have it flown via helicopter or floatplane directly to one of the pack factories like the plant factory visited in Te Anau (Fig 6). The Milford Sound shore based depot can hold up to 2.5 tons while the Te Anau can hold 10 to 14 tons. Once received the lobsters are weighted, graded and 12
  • 13. Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report then swum in the international tanks before export. A minimum swimming time of 24 hours- 48 hours is expected before the lobster is packed in wood wool and ice for transport to either Auckland or Christchurch International Airport. Major markets are China (95 %), Japan (3.9 %) and Dubai (1.1 %), occasionally Italy. Shipments to Asia have mortalities lower than 5 %, in fact customers can only reclaim when mortalities are higher than 5 %. The flight to China lasts up to 35 – 40 h and in fact it has been established that lobster can sustain up to 72 h. Figure 6. Fiordland Lobster Company Operation Map. The blue CRA areas are the rock lobster permit areas in which this company has interest in. 6. Conclusions and future perspectives The study visit to New Zealand was very enlightening as far as research and industry management is concerned. The strong applied research with the financial support of the industry promotes a more competitive and innovative market. It is important to highlight that during my visit I had the unique opportunity to visit 5 prestigious companies. All of them were very interested and helpful. Moreover, I had access to Crop and Food Library, where I could gather much useful information, not only related to research but also to the codes of practice of the Seafood industry training organization. This will be very helpful, considering IPIMAR is currently developing a training program to the Portuguese live crustacean’s traders. Many ideas related to my research area have also taken form and at least a preliminary experiment to test AquiSTM has already been done at the Research Unity of Upgrading Fishery and Aquaculture Products facilities. This preliminary experiment is going to be complemented and scientific communication will take place in short term. 13
  • 14. Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report As far as live crustaceans trade is concerned comparing to Portugal, New Zealand has several advantages that are related to the fact that it is an exporter country specialized in an expensive crustacean, the Rock lobster. Moreover, New Zealand is geographically located near important Asian markets, especially Hong Kong and Japan, a market willing to pay for a supreme quality live lobster. Besides the market advantages, there are technical issues that facilitate the live export: captivity duration is low and water management is specific for one species only. Also, New Zealand has closed depots with a very strict water control. In Portugal, bigger import depots are usually open or semi open systems with a poor water quality management. Some facilities do not cool the water and at least nine species are traded live: four species of crabs, Cancer. pagurus, Maja spp., Necora. puber and Carcinus maenas; two species of clawed lobsters, Homarus gammarus, H. americanus, and at least three species of spiny lobsters Palinurus elephas, P. mauritanicus, and Panulirus regius. The trade of so many different species, with different habitats and physiological needs is a major drawback to settle plant facilities technically suited to keep all these crustaceans live. Also, captivity can be as short as one day and as long as three months depending on species. Though Portugal has a long lasting tradition of keeping live crustaceans in depots, water management is very deficient and attitudes are difficult to change especially considering that this is usually a family business that in some companies dates back from the beginning of the 20th century. In New Zealand this is a recent business where there was a big investment in training; this is just starting in Portugal. Also, since it is an export country the need to settle in the international trade promotes innovation and the search for the necessary improvements to face the market demands. Needless to say that New Zealand has pristine waters where the cool environment and unspoiled coastline provides a fishery haven abundant with high quality seafood. In addition to these unique conditions, fisheries are efficiently managed; access to New Zealand's fishing resources is protected by a 1986 Act of Parliament. The sophisticated Quota Management System controls the harvesting of the natural fish resources to sustain the resources for future generations and is actively supported by the New Zealand fishing industry. 14
  • 15. Sara Isabel da Silva Pires Marques Barrento – FoodFrenz Report Bibliography a) Cawthron annual report, 2007 b) Crop & Food Research, Infosheet No. 2 – 21 2006 c) FAO/WHO (2001) Health and Nutritional Properties of Probiotics in Food including Powder Milk with Live Lactic Acid Bacteria. Report of a Joint FAO/WHO Expert Consultation on Evaluation of Health and Nutritional Properties of Probiotics in Food Including Powder Milk with Live Lactic Acid Bacteria. Sites in the internet: http://www.lobster.co.nz/the_process.html http://www.seafood.co.nz/qms http://www.sealord.co.nz/xml/default.asp?globalPage=C_environment http://www.moanapacific.com/index1.htm http://www.kingsalmon.co.nz/Company/ Acknowledgments I must acknowledge FoodFrenz, all Crop and Food researchers who shared their research and accompanied me to the several companies visited and all managers and technicians of these companies. I must make a special acknowledgement to Dr. Michael Roberts and Doctor Alister Jerret that put me in contact with Dr. Malcom Lawson. Also, a special thanks to Mr. Mike Schuck who kindly received me at Te Anau and Mr. Allan Cosgrove who took part of his Sunday afternoon to meet me at Milford Sound harbour. This visit truly beneficiated my investigation back in Portugal. 15