Floor eggs laid outside of nest boxes pose hygiene issues as they are laid on dirty surfaces. When freshly laid, floor eggs absorb contaminants from the surrounding area through pores in the eggshell. This leads to higher bacterial loads in floor eggs compared to nest eggs. Hatchability is lower for floor eggs due to increased embryonic mortality and rotten eggs. Floor eggs can also cross-contaminate other eggs and hatched chicks in the same incubator. Attempts to disinfect floor eggs through washing or wet disinfection increase health risks rather than improving hatchability. It is recommended that floor eggs not be considered hatching eggs to maintain high biosecurity and chick quality standards.
Viruses are Different From Other Microbes
Viruses are obligate intracellular parasites. They depend totally on their host cells for their existence. Their total host dependence makes it, extremely difficult to get good insight of them natural conditions, because the internal characteristics of the host cells are likely to interfere with the observations. Due to these reasons, it has been found desirable that viruses are cultivated or grown in the laboratory itself.
Laboratory animals
Fertilized Hen’s Egg
Chorioallantoic membrane
Allantoic cavity
Amniotic cavity
Yolk sac
Organ/Tissue/Cell Culture
Growth identified by serological method like neutralization.
Embryonated Egg Chorioallantioc membrane (CAM)
Allantoic cavity
Amniotic cavity
Yolk Sac
Cell Lines/ Tissue cultures Primary
Diploid/ Secondary
Continuous
Animal inoculation Suckling
Embryonated Hen’s Egg
Cultivation of Viruses and Bacteria
Chorioallantoic membrane (CAM) – visible lesions called pocks. Each infectious virus particle forms one pock. e.g. Variola, Vaccinia virus
Allantoic cavity – Influenza virus (vaccine production) & paramyxoviruses
Amniotic cavity – primary isolation of Influenza virus
Yolk sac – Chlamydia, Rickettsia & some viruses
Embryonated eggs:
The Embryonated hen’s egg was first used for cultivation of viruses by Good Pasteur and Burnet (1931). Cultivation of viruses in organized tissues like chick embryo necessitates a different type of approach.. For all practical purposes they all themselves behave as tissue cultures. The process of cultivation of viruses in embryonated eggs depends on the type of egg which is used. The egg used for cultivation must be sterile and the shell should be intact and healthy.
F.M. Burnet in the laboratory
in the early 1950's,
was experimenting
on influenza virus genetics,
using the developing hen's egg
Inoculated eggs are candled
daily to see the chicken
embryos inside.
Animals and chick embryo
were the first method that was used
to cultivate virus. This method is rarely
used as it is not convenient.
However, when preparing for bulk virus,
(e.g. antigen or vaccine production)
the usage of chick embryo is useful.
Fertile chicken eggs provide
a convenient, space-saving incubator
for many kinds of animal viruses.
Different viruses can be injected
into an egg at different sites and
the egg can be easily observed
for viral replication throughout
the development of the chicken embryo.
Isolation and cultivation of many avian
Viruses are Different From Other Microbes
Viruses are obligate intracellular parasites. They depend totally on their host cells for their existence. Their total host dependence makes it, extremely difficult to get good insight of them natural conditions, because the internal characteristics of the host cells are likely to interfere with the observations. Due to these reasons, it has been found desirable that viruses are cultivated or grown in the laboratory itself.
Laboratory animals
Fertilized Hen’s Egg
Chorioallantoic membrane
Allantoic cavity
Amniotic cavity
Yolk sac
Organ/Tissue/Cell Culture
Growth identified by serological method like neutralization.
Embryonated Egg Chorioallantioc membrane (CAM)
Allantoic cavity
Amniotic cavity
Yolk Sac
Cell Lines/ Tissue cultures Primary
Diploid/ Secondary
Continuous
Animal inoculation Suckling
Embryonated Hen’s Egg
Cultivation of Viruses and Bacteria
Chorioallantoic membrane (CAM) – visible lesions called pocks. Each infectious virus particle forms one pock. e.g. Variola, Vaccinia virus
Allantoic cavity – Influenza virus (vaccine production) & paramyxoviruses
Amniotic cavity – primary isolation of Influenza virus
Yolk sac – Chlamydia, Rickettsia & some viruses
Embryonated eggs:
The Embryonated hen’s egg was first used for cultivation of viruses by Good Pasteur and Burnet (1931). Cultivation of viruses in organized tissues like chick embryo necessitates a different type of approach.. For all practical purposes they all themselves behave as tissue cultures. The process of cultivation of viruses in embryonated eggs depends on the type of egg which is used. The egg used for cultivation must be sterile and the shell should be intact and healthy.
F.M. Burnet in the laboratory
in the early 1950's,
was experimenting
on influenza virus genetics,
using the developing hen's egg
Inoculated eggs are candled
daily to see the chicken
embryos inside.
Animals and chick embryo
were the first method that was used
to cultivate virus. This method is rarely
used as it is not convenient.
However, when preparing for bulk virus,
(e.g. antigen or vaccine production)
the usage of chick embryo is useful.
Fertile chicken eggs provide
a convenient, space-saving incubator
for many kinds of animal viruses.
Different viruses can be injected
into an egg at different sites and
the egg can be easily observed
for viral replication throughout
the development of the chicken embryo.
Isolation and cultivation of many avian
Common causes of abortions in dairy animals and their managementveterinary worlds
Common causes of abortions in dairy animals and their management
various bacterial and viral causes of the abortion has been explained with the help of the slide
VIRAL VACCINES
Since viruses are intracellular parasites they will grow only within other living cells.
Methods of viral vaccine production:
Cultivation of virus using free living animals
Fertile eggs
Tissue cultures
How To Change Name On Volaris Ticket.pdfnamechange763
How to change name on Volaris ticket? This is one of the most common questions asked by travelers flying with Volaris Airlines. The mentioned details can help you with your name rectification on the airline ticket. If you are still facing difficulties call the consolidation desk at +1-800-865-1848.
Common causes of abortions in dairy animals and their managementveterinary worlds
Common causes of abortions in dairy animals and their management
various bacterial and viral causes of the abortion has been explained with the help of the slide
VIRAL VACCINES
Since viruses are intracellular parasites they will grow only within other living cells.
Methods of viral vaccine production:
Cultivation of virus using free living animals
Fertile eggs
Tissue cultures
How To Change Name On Volaris Ticket.pdfnamechange763
How to change name on Volaris ticket? This is one of the most common questions asked by travelers flying with Volaris Airlines. The mentioned details can help you with your name rectification on the airline ticket. If you are still facing difficulties call the consolidation desk at +1-800-865-1848.
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1. 15
International Hatchery Practice • Volume 32 Number 4
Floor eggs are eggs that were
laid outside of the breeder
flock’s nest boxes in loose
housing systems. The eggs are often
laid on dirty litter or other contami-
nated surfaces. When an egg is
freshly laid, it still has a temperature
equal to the hen’s body tempera-
ture. It will cool down as it comes
into contact with the cooler air and
surfaces surrounding it in the
breeder house. This causes the egg
contents to shrink, pulling in air
from the surroundings through the
egg pores.
by Dr Carla van der Pol,
Hatchtech, the Netherlands.
www.hatchtech.nl
The cuticle at this point is still
soft and can be penetrated by more
harmful material than outside air.
Therefore, when a warm, fresh egg
is laid on a dirty surface, contami-
nated material may be drawn into
the egg as it cools down.
As a result, floor eggs have a
higher bacterial load than nest eggs.
They constitute a hygiene problem
both within the hatchery and in the
broiler house and would best be
discarded. This may seem to be an
economic loss, but discarding floor
eggs is beneficial to a hatchery’s
quality standards and profitability.
This article aims to explain why we
believe that floor eggs are not
hatching eggs.
Cross contamination
Van den Brand et al. (2016) demon-
strated that hatchability was lower
for floor eggs compared to clean
nest eggs. This was due to lower
fertility, higher embryonic mortality
in the first and second week of
incubation, and a higher percentage
of rotten eggs.
Not only are floor eggs them-
selves affected by the higher micro-
bial load, but they can also cross
contaminate other eggs and hatch-
lings, especially those present in the
same incubator. The most visual
example of this are the so called
‘bangers’ or ‘exploders’.
These are eggs in which the rot-
ting process has caused hydrogen
sulphide to build up, but it is con-
tained by the egg shell and inner
egg shell membranes.
The egg contents may leak
through the pores and at a small
provocation, the egg shell and
membranes may burst like a bal-
loon, directly contaminating neigh-
bouring eggs with a spray of rotten
egg contents and indirectly contam-
inating all other eggs inside (and
sometimes outside) the incubator
with airborne pathogens.
Yolk sac infection
Those chicks that do hatch from
floor eggs have, on average, a lower
body weight than chicks from clean
eggs. Furthermore, they may con-
tribute to post-hatch contamination
of other chicks. Deeming et al.
(2002) found that microbial yolk sac
contamination in embryos that had
died during incubation was three
times as high for floor eggs as it was
for nest eggs. Since they used eggs
with intact egg shells, this suggests
that microbial penetration of
pathogens through the egg shell
into the egg contents does take
place and embryos can already be
affected during incubation.
Since yolk sac infections are a
major cause of early life mortality in
broiler chickens, it can safely be
assumed that a higher contamina-
tion in the egg can also result in
higher mortality in the broiler
house. In particular, when the navels
of contaminated chicks are not
closed optimally, they may spread
the pathogens originating from their
eggs to otherwise clean chickens in
the broiler house.
Risks of floor egg
disinfection
Hatcheries take several measures in
an attempt to lower the contamina-
tion risk of floor eggs. For example,
dirty eggs are sometimes washed
before incubation.
However, washing can damage the
cuticle, making the egg more vulner-
able to pathogenic penetration.
Washing water itself can become a
source of infection when proce-
dures are not closely followed.
In terms of fertility, hatchability,
and percentage of rotten eggs,
washed eggs do not perform better
than floor eggs.
Another method that is often
used is wet disinfection by fogging
sterilising substances around the
eggs. These wet disinfection proce-
dures result in wet eggshells, which
may create the optimal micro cli-
mate for bacterial growth when the
eggs are placed in a warm incuba-
tion environment.
This is a risk especially for eggs
with a high bacterial contamination
from the parent flock. Research by
HatchTech has shown that wet dis-
infection can increase early embry-
onic mortality by up to 3.1%
compared to not disinfecting the
eggs at all. Additionally, the number
of unhatched eggs with pathogenic
contamination was not lower for
wet disinfected eggs than for eggs
without any disinfection.
The eggshell may be clean at the
start of incubation after wet disin-
fection but the egg contents are not
sanitised and hygiene is still a big
issue for embryos and hatched
chicks from floor eggs.
Floor eggs are not hatching
eggs
The best way to deal with floor
eggs is to not consider them to be
hatching eggs. Even when floor eggs
are incubated in a separate machine,
the hatched chicks may spread
pathogenic contamination from
their residual yolk sac during han-
dling and storage or in the broiler
house.
Floor eggs are a problem that
should be tackled at the breeder
level, with good breeder manage-
ment and suitable housing equip-
ment. It is best to take our losses
with any floor eggs that may still
occur if we want to guarantee high
biosecurity and chick quality, not
set them in our incubators. n
References are available
from the author on request
To guarantee chick
quality: floor eggs are
not hatching eggs