Final Double Sided NaR Handout
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This document describes an enzyme-based analytical chemistry method for measuring nitrate levels using nitrate reductase. Key advantages of this method include being non-toxic, accurate, sensitive, selective, and producing fewer interferences than heavy metal-based methods. The method can measure nitrate levels in complex matrices like salt water, soils, and crops. It is offered in various formats tailored for automated analyzers, laboratories, and field testing to provide a green, reliable alternative to methods using cadmium, zinc, or vanadium.
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Final Double Sided NaR Handout
- 1. ENZYME-BASED ANALYTICAL CHEMISTRY N OO N OO O Nitrate Nitrite NITRATE REDUCTASE Replaces Heavy Metals Ships Ambient Assay at Room Temp (22°C) Colorimetric Results Stable for Storage and Use Green Analytical Chemistry Only Reacts with Nitrate Works in Complex Matrices Fewer Interferences Validated Methods Equivalent or Better Results Data Quality Superior NON-TOXIC ACCURATE SENSITIVE SELECTIVE Safe for Users Ecologically Responsible No Hazardous Waste Fees 334 Hecla St, Lake Linden, MI 49945 Technical Support: 906.296.1130 www.nitrate.com NaR Cadmium Wide Detection Range Low Detection Limit Small Sample Volume Specific Binding Site Unique Binding Site False Negatives Rare False Positives Rare SPECIFIC Method D7781 Method N07-0003* CWA 40CFR Part 136 *Anticipated by early 2016 Standard Range Method I-2547-11 Low Range Method I-2578-11 RELIABLE Recombinant Technology Guaranteed >95% Purity Lot-to-Lot Consistency
- 2. NITRATE REDUCTASE PRODUCT FORMATS 334 Hecla St, Lake Linden, MI 49945 Technical Support: 906.296.1130 www.nitrate.com Tailored reagent packs developed hand-in-hand with equipment manufacturers for Discrete Analyzers, SFA, CFA, and some FIA Instruments. Competitive cost per test with reduced waste disposal and no cadmium columns! User provides assay buffer and color reagents. Kit includes enzyme, NADH, and instructions. Simplified kits with prepackaged reagents for on-site and field applications. Specialized kits for water, salt water, soil, crops, and animal forage samples. Customization available for any sample type. Lab quality data anywhere - even for the novice chemist. Enzyme packs for economical routine analysis. Recommended for experienced users. NECi will provide assistance optimizing for various applications. Supplied with enzyme diluent, NADH optional. Replaces methods that use cadmium, zinc, or vanadium - Test safely and with better accuracy Same Griess reaction chemistry for color development Fewer Interferences Suited for salt water, sulfate, humics, grease, and pH extremes Competitive Cost per Test Reduced Cost for Shipping and Disposal Test Tube Format Kits for most equipped labs. The 2 mL assay volume is compatible with most photometers. Results are measured at 540 nm ± 10 nm. Labs supply reagent grade H2O, 3N HCl, and test tubes. The kit supplies the rest. Available in 25 or 100 sample kits in Standard or Low Range. Microplate Format Kits for labs equipped with microplate readers. Standard 96-well microplate included with reagents in kit. Available in Standard or Low range. AUTOMATED ANALYZER REAGENT PACKS ENZYME AND REAGENT PACKS LABORATORY TEST TUBE KITS LABORATORY MICROPLATE KITS SIMPLIFIED FIELD KITS Standard Range: 1.0 - 10.0 ppm Nitrate-N Low Range: 0.05 - 1.0 ppm Nitrate-N Kits for Brackish/Saline Samples