A protein microarray is a high-throughput technology used to study protein interactions, functions, and activities. It's similar to a DNA microarray, but instead of DNA, it uses proteins immobilized on a solid surface (like a glass slide or membrane).

1. Protein Micro
Array
Presented By-
Abhishek Gupta
Presented To-
Dr. Abhishek Awasthi

2. • Protein micro array(or Protein chip) is a high-
throughput method used to track the interactions
&activities of proteins, to determinate their
functions.
• Protein micro array are rapid, automated,
economical and highly sensitive consuming only
small quantities of samples and reagents.

3. • Micro array technology was first developed from
an earlier concept called “Ambient analyte
immunoassay” by Roger Ekins in 1989.
• Later transformed into DNA microarray.
• Due to some limitations in DNA microarray ,
this protein microarray was developed to
overcome those limitations.

5. Protein chip consists of a support surface such as
glass slide, nitrocellulose, bead or microtitreplate to
which array of capture proteins is bound.
Probe molecules typically labeled with a
fluorescent dye are added to the array.
Any reaction between the probe and the
immobilized protein emits a fluorescent
signal and that is read by laser scanner.

7. șecondary• .AB
Primary AB
Antigen capture

8. NCTI PR TEIN
Protein –DNA
interactions
Protein – Small
molecule
Kinase Immune
responses

9. • Functional protein microarray are(target
protein array) constructed by immobilizing
large number of proteins to assay enzymatic
activity and to detect antibodies & their
functions.
• They differ from analytical protein microarray
by containing full length functional proteins or
protein domains.

10. • These are used to study the Biochemical
activities 1
"
of entire proteome in a single
experiment.
• The proteins must retain their native or
original structure so that meaningful
functional interactions takes place on the
array surface.

12. LABEL - DEPENDENT LABLE- FREE
Several types of labeling
reagents like fluorescent
dyes(Cy3 & Cy5).
•Label dependent may effect the
protein activity.
•Surface Plasmon Resonance
Spectroscopy (SPRS)
•Optical Elipsometry (OE)
•Reflectometric Interference
Spectroscopy(RIFS)
•Oblique-incidence reflectivity
difference(OIRD)
ensymes(Horseradish peroxides),
radio isotopes (32P, 33P &14C
liposome's are used.
4•Rolling circle amplication (RCA)
Tyramide signal amplication(TSA)
used to detect low abundance
proteins.

13. Application
Mainly used in 5 major areas
➢ Diagnostics
➢ Proteomics
➢ Protein functional analysis
➢ Antibody characterization
➢ Treatment development

14. nos
Detection in antigen antibodies in blood sample
To discover new disease biomarkers; monitoring the
diseased state and responses to therapy.
Ex: Digital bioassay
eo ics:
Protein expression profiling i.e., which proteins are
expressed in a particular cell.

15. ein nc
To identi
▪ Protein-protein interactions
▪ Protein- phospholipids interactions
▪ Enzymatic substrates
▪ Receptor ligands

16. eriza ion:
❑Characterization of cross reactivity
specificity and mapping epitopes.
rea en eve en
❑Development of antigen- specificity therapies for
autoimmunity , cancer and allergies.
❑Identification of small molecules that could potentially
used to be as new drugs