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EXPERIENCE AT GENERAL
BIOLOGY I LABORATORY
By Jose A. Cruz Arzon
CHEMISTRY OF LIFE
SOLUTION PREPARATION
Glucose Stock Solution
Stock solution at different
concentrations
DETECTING MACROMOLECULES
Benedict Test: positive to carbohydrates are red,
orange, yellow and green.
Unknown
Glucose
Milk
Coconut
Onion
Juice
Biuret Test: positive to proteins are presented in
violet.
Casein
Milk
Coconut
Albumin
Lugol Test: postive to starch are presented in
black.
Starch
TRANSPORT IN THE CELL
MEMBRANE
Inside the cell: glucose and starch. Glucose
stays inside the cell after an hour.
Inside the cell: no protein (albumin).
Albumin stays outside the cell after an hour.
Inside the cell: Starch. Starch stays inside
the cell after an hour.
Outside the cell: no glucose. Glucose
can cross the lipid bilayer
Outside the cell: albumin. Albumin stays
outside the cell.
Outside the cell: Lugol. Lugol can cross
the lipid bilayer, since it detected the
starch that was inside.
ENZYME ACTIVITY
Enzyme activity of celobiase at different substrate concentrations
PHOTOSYNTHESIS
Coleus sp. (plant used)
Polar and non-polar pigments extraction from Coleus leaf
Chromatography for the separation of
pigments
Light dependent and Light independent reaction in Elodea using phenol red.
CELLULAR RESPIRATION
Measuring respiration rate in a water snail and a goldfish using the
Principle of Tritation
Fermentation of yeast in substances like water, corn syrup and sucrose
MOLECULAR GENETICS
DNA samples for Agarose Gel Electrophoresis
Results of my first Agarose Gel
Electrophoresis
DNA extraction from a strawberry
MENDELIAN GENETICS
Corn used to measure the rate of
dominant and recessive alleles
P.T.C Paper to see how many students are dominant for bitter flavor
and recessive for tasteless.

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Experience at general biology i laboratory