This document contains environmental data from a sample collected on March 4th 2013 at 6:15 AM in a rural area at coordinates 18.17336, -65.86055. The temperature was 25°C, depth was 2.5 cm, and soil was dry. It also describes enrichment and streaking protocols carried out on March 5th and 11th 2013 and two rounds of plaque purification on April 3rd and 4th 2013. Preliminary results found two successful rounds of purification with a third planned and positive soil samples seeming to come from areas with dark soil and high plant density.

1. Edwin J. Alvarado
José Antonio Cruz-Arzón

2. Environmental Data
“Sample 3”
Date: 2013 March 4th 6:15 A.M.
Data: Location: 18.17336, –65.86055
Temperature: 25 ºC
Depth: 2.5 cm
Moisture: Dry
Geography: Rural Area
Water Proximity

3. Enrichment & Streaking Protocol
Date: 2013 March 5th
Date: 2013 March 11th

4. Plaque Purification
Plaque
Purification I
PP 1.1
PP 1.2
Date: 2013 April 3rd

5. Plaque Purification
Plaque
Purification II
Plaque
Purification I
PP 1.1
PP 1.2
PP 2.1.2
PP 2.2.1
PP 2.2.2
Date: 2013 April 4th

6. Preliminary Results & Conclusion
Results include two successful rounds of purification.
Third purification to be carried out in the coming weeks.
Positive soil samples seem to derive from areas with
dark soil and a high plant density.