The document provides an overview of ELISA (Enzyme-Linked Immunosorbent Assay), describing it as an antibody-based method used to quantitatively or qualitatively detect specific antigens or antibodies. It discusses different types of ELISA including indirect, sandwich, and ELISPOT. The basic principles of two-step and sandwich ELISA are described, involving binding of primary and secondary antibodies and use of an enzyme to produce a color change indicating the presence of an analyte. Considerations for whether ELISA is suitable for a given experiment are also outlined.