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EFFECTS OF UREA AND ADULT PALM FERTILIZER MIXTURE ON THE SURVIVAL AND BODY WALL TISSUES OF THE EARTHWORM, Pheretima hawayana O. K. C. DE SILVA Department of Zoology University of Kelaniya
Introduction ,[object Object],[object Object],[object Object]
Introduction cont’d… ,[object Object],[object Object]
[object Object],[object Object],[object Object]
Field survey; Study area
Study sites Site A – Home garden  Site B – Banana plantation
Study sites cont’d… Site C– Coconut plantation Site D – Pineapple plantation
Field survey cont’d… ,[object Object],[object Object],[object Object]
Results of the  Field Survey Variation of the abundance of  Notoscolex  spp between the four study sites Abundance of  Notoscolex  spp  ± SE
Field survey cont’d… ,[object Object],[object Object],[object Object],[object Object]
Results of the  Field Survey  cont’d… Variation of soil pH between the four field study sites
Laboratory Experiment ,[object Object],Pheretima hawayana
Laboratory Experiment cont’d… ,[object Object],[object Object]
Group of replicate Urea treatment C1 (n=4) 1.5g of urea per container(30 g m -2 ) C2 (n=4 ) 0.1g of urea per container(1.96 g m -2 ) C3 (n=4 ) 3.0g of urea per container(58.8 g m -2 ) C4 (n=4 ) C0 (n=4 ) 16.2g of urea per container(317.6 g m -2 ) Control treatment
Laboratory Experiment cont’d… ,[object Object],[object Object]
[object Object],first week (  ), second week (  ) and third week (  ) % Survival of  P. hawayana   ± SE
Laboratory Experiment cont’d… ,[object Object],[object Object]
Group of replicate APM fertilizer treatment C1 (n=4) 2.2 g of fertilizer per container (43.2 g m -2 ) C2 (n=4 ) 1.0 g of fertilizer per container (19.6 g m -2 ) C3 (n=4 ) 5.0 g of fertilizer per container (98.0 g m -2 ) C4 (n=4 ) C0 (n=4 ) 21.6 g of fertilizer per container (424.3 g m -2 ) Control treatment
first week (  ), second week (  ), third week (  ) and fourth week (  )  % Survival of  P. hawayana  ± SE Applied APM fertilizer amount (g m -2 ) Variation of the % survival of  P. hawayana  in different APM fertilizer treatments
Results of The Laboratory Experiment cont’d… ,[object Object],[object Object]
Histological experiment ,[object Object],[object Object],[object Object],[object Object],[object Object]
Histological experiment cont’d… Control Urea added APM fertilizer added CU EL EL CM CM EL CM CM - circular muscle layer  CU - cuticle  EL - epithelial layer
Conclusions ,[object Object],[object Object]
[object Object],[object Object]
Thank You

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Effects of urea and adult palm fertilizer mixture on the survival and body wall tissues of earthworms

  • 1. EFFECTS OF UREA AND ADULT PALM FERTILIZER MIXTURE ON THE SURVIVAL AND BODY WALL TISSUES OF THE EARTHWORM, Pheretima hawayana O. K. C. DE SILVA Department of Zoology University of Kelaniya
  • 2.
  • 3.
  • 4.
  • 6. Study sites Site A – Home garden Site B – Banana plantation
  • 7. Study sites cont’d… Site C– Coconut plantation Site D – Pineapple plantation
  • 8.
  • 9. Results of the Field Survey Variation of the abundance of Notoscolex spp between the four study sites Abundance of Notoscolex spp ± SE
  • 10.
  • 11. Results of the Field Survey cont’d… Variation of soil pH between the four field study sites
  • 12.
  • 13.
  • 14. Group of replicate Urea treatment C1 (n=4) 1.5g of urea per container(30 g m -2 ) C2 (n=4 ) 0.1g of urea per container(1.96 g m -2 ) C3 (n=4 ) 3.0g of urea per container(58.8 g m -2 ) C4 (n=4 ) C0 (n=4 ) 16.2g of urea per container(317.6 g m -2 ) Control treatment
  • 15.
  • 16.
  • 17.
  • 18. Group of replicate APM fertilizer treatment C1 (n=4) 2.2 g of fertilizer per container (43.2 g m -2 ) C2 (n=4 ) 1.0 g of fertilizer per container (19.6 g m -2 ) C3 (n=4 ) 5.0 g of fertilizer per container (98.0 g m -2 ) C4 (n=4 ) C0 (n=4 ) 21.6 g of fertilizer per container (424.3 g m -2 ) Control treatment
  • 19. first week ( ), second week ( ), third week ( ) and fourth week ( ) % Survival of P. hawayana ± SE Applied APM fertilizer amount (g m -2 ) Variation of the % survival of P. hawayana in different APM fertilizer treatments
  • 20.
  • 21.
  • 22. Histological experiment cont’d… Control Urea added APM fertilizer added CU EL EL CM CM EL CM CM - circular muscle layer CU - cuticle EL - epithelial layer
  • 23.
  • 24.