PREPARATION AND APPLICATION OF PANCREATIN COATED ZINC NANOPARTICLE AGAINST METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS(MRSA)

1. PREPARATION AND APPLICATION OF
PANCREATIN COATED ZINC NANOPARTICLE
AGAINST METHICILLIN RESISTANT
STAPHYLOCOCCUS AUREUS(MRSA)
PRESENTED BY,
JYOTSNA MONDAL
DEBOLINA MUKHERJEE
NEHA KUMARI

2. ABSTRACT
 Antimicrobial nanoparticles represent a promising
alternative to antibiotics and may lower antibiotic
use and spread of antibiotic resistance traits along
pathogens.
 Zinc nanoparticle coated with pancreatin digest are
experinced against MRSA.

3. CONTINUED..
 The highest in vitro inhibitory effect of our NP
was obsereved against methicillin resistant
Staphylococcus aureus.
 After successful in vitro testing,in vivo study
with goat skin was conducted.

4. INTRODUCTION
 S. aureus infections range in extremities from
simple skin lesions to life threatening infections
like pneumonia, endocarditis, osteomyelitis,
septicemia and exotoxins syndromes .
 Prolonged usage of bacteriostatic or bactericidal
antibiotics has resulted in the emergence of
multidrug resistance strains (MDR) .The ability of
MRSA to endure conventional antibiotic
treatment can be attributed to its biofilm
forming capability

5. MRSA (methichillin resistant
staphylococcus aureous):

6. Infections caused by MRSA:
Plasma Agglutination by MRSA:

7. CONTINUED
 Due to the high exposure of bacteria with
different antibiotics,they are developing
resistance aginst it.Multidrug resistivity
(MDR) enable some bacteria to oppose the
action of certain antibiotics,rendering the
antibiotics ineffective.
 To overcome this problem we can use the
natural compounds as nanoparticles, which is
also cost efficient

8. CONTINUED
 Oxidative stress Background Zinc-based
nanoparticles have been applied in several
fields including agriculture, chemistry, textile
and food industry, electronics, and medicine
 Due to their antibacterial activity, the Zn
nanoparticles (NPs), particularly ZnO-based,
have been designed and tested against
diverse bacterial species, including pathogen,
Staphylococcus aureus, and antibiotic-
resistant strains including methicillin resistant
S.aureus (MRSA)

9. CONTINUED
 The exact mode of action of Zn NPs is not
well understood;it can be either by
generating reactive oxygen species (ROS) or
by disrupting bacterial cell wall after the
contact with nanoparticles.
 Due to ROS production, the ZnO NPs cause
oxidative stress which leads to inflammation
and even death of mammalian cell lines.

10. Zinc Nanoparticles:
Pancreatin enzymes:

11. DLS peak for Zinc nanoparticles:
DLS peak
for
Zinc-
pancreatin
nanoparticles:

12. CONTINUED
 In the present study, enzyme -based NP
formulation was synthesized and
characterized.
 After confirmation of their antibacterial
activity in vitro, the study on goat skin was
conducted.The in vivo test aimed to
determine whether the selected enzyme-
based NP has comparable effect to ZnO and
ZnO NPs on the skin microbiome or not.

13. CONTINUED..
 Zinc is present in our body and pancreatin is a
naturally occuring enzyme.Hence the zinc
pancreatin nanoparticle is neither harmful nor
toxic for health.Working with it’s low
concentration helps in killing skin infection
causing MRSA.

14. CONTINUED..
 Pancreatin is an enzymtic digest of
amylase,lipase and protease which enhances
the antimicrobial property of the nanoparticle.
Being heat labile, it is added after calcination
of zinc nanoparticle which coats the ZnO NP
by sonication.

15. CONTINUED
 Biofilms on surfaces have a characteristic
structure consisting of microcolonies enclosed in
a hydrated matrix of microbially produced
proteins, nucleic acids, and polysaccharides.
Here the cells act less as individual entities and
more as a collective living system.

16. Processes inclusive to
biofilms of MRSA:

17. CONTINUED
 Biofilm organisms are significantly more
resistant to environmental stresses or
microbially deleterious substances like
antibiotics and biocides, than planktonic
cells. So for the controlling of those bacteria
we have to kill or inhibit their biofilm
formation.
 The nanoparticle we used ,have antimicrobial
affect on MRSA as it inhibit MRSA to form
biofilm ,hence it inhibit bacteria to cause skin
infection.

18. DISCUSSION
 As previously seen, zinc nanoparticle is
characterized by DLS, spectroscan method.
 Affectivity of the nanoparticle is proved by
several tests like disc diffusion, mic checking,
virulence property etc.
 The results show promising efficiency of the
nanoparticle to inhibit biofilm formation by
MRSA.

19. CONTINUED..
 To confirm the ability of the nanoparticle we
apply this on goat skin with skin infection
causing MRSA.