This document provides a training module on measuring silicate in water samples. It includes background on silicate occurrence and the ammonium molybdate spectrophotometric method. The module consists of a theoretical session, practical laboratory session to analyze samples using the standard analytical procedure, and a report writing session. The goal is for participants to understand the importance of silicate measurement and be able to analyze water samples for silicate concentration.
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1. World Bank & Government of The Netherlands funded
Training module # WQ - 46
How to Measure Silicate
New Delhi, September 2000
CSMRS Building, 4th Floor, Olof Palme Marg, Hauz Khas,
New Delhi – 11 00 16 India
Tel: 68 61 681 / 84 Fax: (+ 91 11) 68 61 685
E-Mail: dhvdelft@del2.vsnl.net.in
DHV Consultants BV & DELFT HYDRAULICS
with
HALCROW, TAHAL, CES, ORG & JPS
2. HP Training Module File: “ 46 How to Measure Silicate.doc” Version: September 2000 Page 1
Table of contents
Page
1. Module context 2
2. Module profile 3
3. Session plan 4
4. Overhead/flipchart master 5
5. Evaluation sheets 9
6. Handout 11
7. Additional handout 14
8. Main text 16
3. HP Training Module File: “ 46 How to Measure Silicate.doc” Version: September 2000 Page 2
1. Module context
This module introduces importance of silicate in natural waters, and measurement of silicate
in water samples by ammonium molybdate spectrophotometric method.
While designing a training course, the relationship between this module and the others,
would be maintained by keeping them close together in the syllabus and place them in a
logical sequence. The actual selection of the topics and the depth of training would, of
course, depend on the training needs of the participants, i.e. their knowledge level and skills
performance upon the start of the course.
No. Module title Code Objectives
1. Basic water quality concepts WQ - 01 • Discuss the common water quality
parameters
• List important water quality issues
2. Basic chemistry concepts WQ - 02 • Convert units from one to another
• Discuss the basic concepts of
quantitative chemistry
• Report analytical results with the
correct number of significant digits.
3. The need for Good
Laboratory Practice
WQ – 03 • Follow approved general laboratory
procedures
4. How to prepare standard
solutions
WQ – 04 • Select different types of glassware
• Use an analytical balance and
maintain it
• Prepare standard solutions
5. Basic Aquatic Chemistry
concept
WQ – 24 • Calculate ion concentrations from
ionisation constants
6. Major ions in water WQ - 28 • Know the major ions in water and air
sources
• Understand the significance of major
ion concentrations
7. Absorption Spectroscopy WQ - 34 • Understand the principle of absorption
spectroscopy
• Explain the use of absorption
spectroscopy for chemical analyses
4. HP Training Module File: “ 46 How to Measure Silicate.doc” Version: September 2000 Page 3
2. Module profile
Title : How to Measure Silicate
Target group : HIS function(s): Q2, Q3, Q5, Q6
Duration : 1 theoretical session of 30 min, plus
1 Practical Laboratory session of 120 min, plus
1 Report writing session of 30 min.
Objectives : After the training the participants will be able to:
• Understand the relevance of silicate to water quality
• Analyse water samples for silicate
Key concepts : • Molybdosilicate method for silicate
Training methods : Lecture, Laboratory Analytical Exercise, Report preparation
Training tools
required
: Board, flipchart, OHS, Complete Laboratory Facilities for silicate
Analysis
Handouts : As provided in this module including SAP for Analysis of Silicate
Further reading
and references
: • Chemistry for environmental engineers - C. N. Sawyer, P. L.
McCarty & G. F. Parkin, McGraw - Hill, Inc., 1994
• Standard methods for the examination of water and
wastewaters, AWWA, 19th
edition, 1995
5. HP Training Module File: “ 46 How to Measure Silicate.doc” Version: September 2000 Page 4
3. Session plan
No Activities Time Tools
1 Preparations
• Prepare reagents required for analysis according to SAP
• Prepare samples as follows:
A – Groundwaer
B and C – Using distilled water and silica stock solution
prepare samples containing 0.8 and 3 mg/L silica
2 Introduction:
• Introduce the session
• Ask the question: “Why do we need to measure
silicate?”
• Talk about the occurrence of silicate in the environment
10 min
List answers
on flip chart
OHS
3 Ammonium Molybdate Spectrophotometric Method –
Background:
• Discuss the ammonium molybdate method in terms of
its chemistry, the theory of spectrophotometry and
potential interferences
20 min OHS
4 Practical Session:
• Allow participants to conduct the silicate analysis
according to SAP: Refer to SAP for silicate
• Stress the need to write up material as the analysis is
proceeding
• Be available to guide participants and answer questions
120 min OHS
5 Report Writing:
• Allow participants to complete their reports
• Give the ‘correct’ answers to the silicate analyses
• Discuss results
30 min Use flip
chart
6. HP Training Module File: “ 46 How to Measure Silicate.doc” Version: September 2000 Page 5
4. Overhead/flipchart master
OHS format guidelines
Type of text Style Setting
Headings: OHS-Title Arial 30-36, with bottom border line (not:
underline)
Text: OHS-lev1
OHS-lev2
Arial 24-26, maximum two levels
Case: Sentence case. Avoid full text in UPPERCASE.
Italics: Use occasionally and in a consistent way
Listings: OHS-lev1
OHS-lev1-Numbered
Big bullets.
Numbers for definite series of steps. Avoid
roman numbers and letters.
Colours: None, as these get lost in photocopying and
some colours do not reproduce at all.
Formulas/Equat
ions
OHS-Equation Use of a table will ease horizontal alignment
over more lines (columns)
Use equation editor for advanced formatting
only
7. HP Training Module File: “ 46 How to Measure Silicate.doc” Version: September 2000 Page 6
Silicon
• Next to oxygen in abundance in earth’s crust
• Silica (SiO2) in sand and quartz
• Silicate Minerals
• Causes scaling problems
8. HP Training Module File: “ 46 How to Measure Silicate.doc” Version: September 2000 Page 7
Molybdosilicate Method
• Silica reacts with molybdate to give yellow colour
• Phosphate also undergoes similar reaction
- oxalic acid is added to destroy colour due to phosphate
• Colour and turbidity of sample may also interfere
- run suitable blank for compensation
• Read colour at 815 nm
• Dilute sample if silica > 2 mg/L
9. HP Training Module File: “ 46 How to Measure Silicate.doc” Version: September 2000 Page 8
Experiment
• Aim:
- To determine silicate in different samples of water
• Method:
- Collect samples A, B and C
- Dilute sample if out of range
- Use SAP
• Report:
- Discuss observed silicate conc. in terms of water quality
10. HP Training Module File: “ 46 How to Measure Silicate.doc” Version: September 2000 Page 9
5. Evaluation sheets
11. HP Training Module File: “ 46 How to Measure Silicate.doc” Version: September 2000 Page 10
12. HP Training Module File: “ 46 How to Measure Silicate.doc” Version: September 2000 Page 11
6. Handout
13. HP Training Module File: “ 46 How to Measure Silicate.doc” Version: September 2000 Page 12
Measurement of Silicate
• Next to oxygen in abundance in earth’s crust
• Silica (SiO2) in sand and quartz
• Silicate minerals
• Causes scaling problems
Molybdosilicate Method
• Silica reacts with molybdate to give yellow colour
• Phosphate also undergoes similar reaction
- oxalic acid is added to destroy colour due to phosphate.
• Colour and turbidity of sample may also interfere
- Run suitable blank for compensation
• Read colour at 815 nm.
• Dilute sample if silica > 2 mg/L.
Experiment
• Aim
- To determine silicate in different samples of water
• Method
- Collect samples A, B and C
- Dillute Sample if out of range
- Use SAP
• Report
- Discuss observed silicate conc. in terms of water quality.
14. HP Training Module File: “ 46 How to Measure Silicate.doc” Version: September 2000 Page 13
Add copy of Main text in chapter 8, for all participants.
15. HP Training Module File: “ 46 How to Measure Silicate.doc” Version: September 2000 Page 14
7. Additional handout
These handouts are distributed during delivery and contain test questions, answers to
questions, special worksheets, optional information, and other matters you would not like to
be seen in the regular handouts.
It is a good practice to pre-punch these additional handouts, so the participants can easily
insert them in the main handout folder.
16. HP Training Module File: “ 46 How to Measure Silicate.doc” Version: September 2000 Page 15
17. HP Training Module File: “ 46 How to Measure Silicate.doc” Version: September 2000 Page 16
8. Main text
Contents
1. Introduction 1
2. Molybdosilicate Method 1
3. Experiment 1
SAP for Silicate 3
18. HP Training Module File: “ 46 How to Measure Silicate.doc” Version: September 2000 Page 1
How to Measure Silicate
1. Introduction
Silicon occurs as the oxide (silica) in quartz and sand and in the form of complex silicate
minerals, particularly igneous rocks. Silica occurs in natural waters as suspended particles,
in a colloidal or polymaric state and as silicilic acids as silicate ions. Its concentration ranges
between 1 – to 30 – mg/L.
Silica forms scales in boilers, which is difficult to remove. Silica and silicate scales
particularly formed on high pressure steam turbine blades are troublesome.
2. Molybdosilicate Method
Ammonium molybdate at low pH reacts with silica and any phosphate present to produce
hetropoly acids giving a yellow colour. Oxalic acid is added to destroy the molybdo
phosphoric acid. The intensity of the colour can be measured at 815 nm. In atleast one of
its forms, the silica does not react with molybdate. To detect unreactive, silica the sample
may be digested with sodium bicarbonate. The method can detect upto 0.2 mg/L silica.
Samples containign more than 10 mg/L silica should be diluted.
Interference due to colour and turbidity may be compensated by running a parallel blank
without addition of molybdate, which should be used to adjust the spectrophotometers for
zero absorbance.
3. Experiment
Aim
a. To determine the concentration of silicate ion in a number of different samples by
spectrophotometry
Method
a. Collect a sample from each of the buckets marked A, B and C.
b. Determine the silicate in each sample according to the Standard Analytical Procedure for
silicate, ammonium molybdate spectrophotometric method.
Observations & calculations
a. Fill in the following table as you proceed with the method:
Standard or Sample Absorbance at 815 nm (R)
0.4 mg/l Standard
0.8 mg/l Standard
1.2 mg/l Standard
1.6 mg/l Standard
2.0 mg/l Standard
A
B
C
19. HP Training Module File: “ 46 How to Measure Silicate.doc” Version: September 2000 Page 2
b. Use the values of the standard solutions in the table to plot a graph of absorbance vs.
silicate concentration.
c. Read the silicate concentration of the three samples from the standard curve.
Report
When writing your report the following aspects should be addressed:
• the aim of the investigation
• the results that you have produced
• the silicate concentration of the samples and what this could mean in terms of water
quality
20. HP Training Module File: “ 46 How to Measure Silicate.doc” Version: September 2000 Page 3
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