This document provides materials for a training module on measuring total iron concentration in water samples. It includes an introduction to iron in water and the phenanthroline spectrophotometric method. The document outlines objectives, key concepts, required materials and references. It provides a session plan, presentation slides, evaluation sheets, handouts and the main text describing the experiment and analytical procedure. The experiment aims to determine total iron in different water samples using spectrophotometry. Participants will analyze samples, record results, plot a calibration curve and report on the suitability of the samples for drinking water use.
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1. World Bank & Government of The Netherlands funded
Training module # WQ - 43
How to Measure Total Iron
New Delhi, September 2000
CSMRS Building, 4th Floor, Olof Palme Marg, Hauz Khas,
New Delhi – 11 00 16 India
Tel: 68 61 681 / 84 Fax: (+ 91 11) 68 61 685
E-Mail: dhvdelft@del2.vsnl.net.in
DHV Consultants BV & DELFT HYDRAULICS
with
HALCROW, TAHAL, CES, ORG & JPS
2. HP Training Module File: “ 43 How to Measure Total Iron .doc” Version: September 2000 Page 1
Table of contents
Page
1. Module context 2
2. Module profile 3
3. Session plan 4
4. Overhead/flipchart master 5
5. Evaluation sheets 12
6. Handout 14
7. Additional handout 17
8. Main text 19
3. HP Training Module File: “ 43 How to Measure Total Iron .doc” Version: September 2000 Page 2
1. Module context
This module deals with the significance of iron to water quality and method for measuring
iron in water samples. Modules in which prior training is required to complete this module
successfully and other available, related modules in this category.
While designing a training course, the relationship between this module and the others,
would be maintained by keeping them close together in the syllabus and place them in a
logical sequence. The actual selection of the topics and the depth of training would, of
course, depend on the training needs of the participants, i.e. their knowledge level and skills
performance upon the start of the course.
No. Module title Code Objectives
1. Basic water quality concepts WQ - 01 • Discuss the common water quality
parameters
• List important water quality issues
2. Basic chemistry concepts WQ - 02 • Convert units from one to another
• Discuss the basic concepts of
quantitative chemistry
• Report analytical results with the
correct number of significant digits.
3. The need for Good
Laboratory Practice
WQ – 03 • Follow approved general laboratory
procedures
4. How to prepare standard
solutions
WQ – 04 • Select different types of glassware
• Use an analytical balance and
maintain it
• Prepare standard solutions
5. Basic Aquatic Chemistry
concept
WQ – 24 • Calculate ion concentrations from
ionisation constants
6. Major ions in water WQ - 28 • Know the major ions in water and air
sources
• Understand the significance of major
ion concentrations
7. Absorption Spectroscopy WQ - 34 • Understand the principle of absorption
spectroscopy
• Explain the use of absorption
spectroscopy for chemical analyses
4. HP Training Module File: “ 43 How to Measure Total Iron .doc” Version: September 2000 Page 3
2. Module profile
Title : How to Measure Total Iron
Target group : HIS function(s): Q2, Q3, Q5, Q6
Duration : 1 Theoretical session of 30 min, plus
1 Practical Laboratory session of 120 min, plus
1 Report writing session of 30 min.
Objectives : After the training the participants will be able to:
• Understand the relevance of iron to water quality
• Know how to make analysis of iron in water samples.
Key concepts : • Phenanthroline method
Training methods : Lecture, Laboratory Analytical Exercise, Report preparation
Training tools
required
: Board, flipchart, OHS, Complete Laboratory Facilities for Iron
Analysis
Handouts : As provided in this module,
Including SAP for analysis of Iron
Further reading
and references
: • Chemistry for environmental engineers - C. N. Sawyer, P. L.
McCarty & G. F. Parkin, McGraw - Hill, Inc., 1994
• Standard methods for the examination of water and
wastewaters, AWWA, 19th
edition, 1995
5. HP Training Module File: “ 43 How to Measure Total Iron .doc” Version: September 2000 Page 4
3. Session plan
No Activities Time Tools
1 Preparations
• Prepare reagents according to SAP for iron
measurement
• Prepare samples A, B & C, containing approximate
0.1, 1.0 and 4 mg/L iron, respectively, using tap
water and appropriate standard solution
2 Introduction
• Introduce the session
• Ask the question, ‘Why do we need to measure iron’?
• Introduce the subject of iron to the participants
including occurrence, concentrations, problems and
standards
10 min
OHS
3 Phenanthroline Spectrophotometric Method –
Background
• Describe the method in terms of its chemistry and
how iron is measured: Refer to SAP for Iron
• Explain the overall aim of this module
• Ask participants to read SAP for iron determination
20 min OHS
4 Practical Session
• Allow participants to conduct analysis according to
SAP: Refer to SAP of Iron
• Stress the need to write-up results as the analysis
proceeds
• Be available to guide participants and answer
questions
120 min
5 Report Writing:
• Allow participants to complete their reports
• Discuss results
30 min
6. HP Training Module File: “ 43 How to Measure Total Iron .doc” Version: September 2000 Page 5
4. Overhead/flipchart master
OHS format guidelines
Type of text Style Setting
Headings: OHS-Title Arial 30-36, with bottom border line (not:
underline)
Text: OHS-lev1
OHS-lev2
Arial 24-26, maximum two levels
Case: Sentence case. Avoid full text in UPPERCASE.
Italics: Use occasionally and in a consistent way
Listings: OHS-lev1
OHS-lev1-Numbered
Big bullets.
Numbers for definite series of steps. Avoid
roman numbers and letters.
Colours: None, as these get lost in photocopying and
some colours do not reproduce at all.
Formulas/
Equations
OHS-Equation Use of a table will ease horizontal alignment
over more lines (columns)
Use equation editor for advanced formatting
only
7. HP Training Module File: “ 43 How to Measure Total Iron .doc” Version: September 2000 Page 6
Measurement of Iron
• Iron is abundant in earth’s crust
• Concentrations are normally low (< 0.1 mg/L) in surface
waters but can be higher in groundwaters (up to 10 mg/L or
more)
8. HP Training Module File: “ 43 How to Measure Total Iron .doc” Version: September 2000 Page 7
Measurement of Iron
• Elevated concentrations of iron in drinking water can cause
problems due to:
- staining of laundry
- unpleasant appearance
- unpleasant taste
• India limits on iron are:
- 0.3 mg/L for water to be used for drinking without treatment
- 50 mg/L for raw water to be used for drinking after conventional
treatment
9. HP Training Module File: “ 43 How to Measure Total Iron .doc” Version: September 2000 Page 8
Measurement of Iron
• Phenanthroline Spectrophotometric Method:
- Relies on the fact that iron ion forms a stable complex with
phenanthroline
- The absorbance of the red complex produced is measured in a
spectrophotometer at wavelength of 510 nm
- Iron concentration is read from a calibration curve
10. HP Training Module File: “ 43 How to Measure Total Iron .doc” Version: September 2000 Page 9
Experiment
• To determine the concentration of total iron in a number of
different samples by spectrophotometry
11. HP Training Module File: “ 43 How to Measure Total Iron .doc” Version: September 2000 Page 10
Experiment
Method:
• Collect a sample from each of the buckets A, B and C
• Determine total iron in each sample
• Samples containing more than 200 mg in 50 ml aliquot will
need adequate dilution
12. HP Training Module File: “ 43 How to Measure Total Iron .doc” Version: September 2000 Page 11
Experiment
Report:
• The three samples represent potential drinking waters with
different levels of iron. On the basis of your result state
whether they are:
- Unsuitable for drinking water use
- Suitable for drinking water use after conventional treatment
- Suitable for drinking water use without treatment
13. HP Training Module File: “ 43 How to Measure Total Iron .doc” Version: September 2000 Page 12
5. Evaluation sheets
14. HP Training Module File: “ 43 How to Measure Total Iron .doc” Version: September 2000 Page 13
15. HP Training Module File: “ 43 How to Measure Total Iron .doc” Version: September 2000 Page 14
6. Handout
16. HP Training Module File: “ 43 How to Measure Total Iron .doc” Version: September 2000 Page 15
Measurement of Iron
• Iron is abundant in the earth’s crust
• Concentrations are normally low (<0.1 mg/L) in surface waters but can be higher
in groundwaters (up to 10 mg/L or more)
• Elevated concentrations of iron in drinking water can cause problems due to:
- staining of laundry
- unpleasant appearance
- unpleasant taste
• India limits on iron are:
- 0.3 mg/L for water to be used for drinking without treatment
- 50 mg/L for raw water to be used for drinking after conventional treatment
• Phenanthroline Spectrophotometric Method:
- Relies on the fact that iron ion forms a stable complex with phenanthroline
- The absorbance of the red complex produced is measured in a
spectrophotometer at a wave length of 510 nm
- Iron concentration is read from a calibration curve
Experiment:
• To determine the concentration of total iron in a number of different samples by
spectrophotometry
Method:
• Collect a sample from each of the buckets A, B and C
• Determine total iron in each sample
• Samples containing more than 200 mg in 50 ml aliquot will need adequate
dilution
Report:
• The three samples represent potential drinking waters with different levels of iron.
On the basis of your result state whether they are:
- Unsuitable for drinking water use
- Suitable for drinking water use after conventional treatment
- Suitable for drinking water use without treatment
17. HP Training Module File: “ 43 How to Measure Total Iron .doc” Version: September 2000 Page 16
Add copy of Main text in chapter 8, for all participants.
18. HP Training Module File: “ 43 How to Measure Total Iron .doc” Version: September 2000 Page 17
7. Additional handout
These handouts are distributed during delivery and contain test questions, answers to
questions, special worksheets, optional information, and other matters you would not like to
be seen in the regular handouts.
It is a good practice to pre-punch these additional handouts, so the participants can easily
insert them in the main handout folder.
19. HP Training Module File: “ 43 How to Measure Total Iron .doc” Version: September 2000 Page 18
20. HP Training Module File: “ 43 How to Measure Total Iron .doc” Version: September 2000 Page 19
8. Main text
Contents
1. Introduction 1
2. Phenanthroline Spectrophotometric Method 1
3. Experiment 1
SAP for Iron (1.13) 3
21. HP Training Module File: “ 43 How to Measure Total Iron .doc” Version: September 2000 Page 1
How to Measure Total Iron
1. Introduction
Iron is abundant element in the earth’s crust but, assuming the pH of surface waters it is
between 6 and 9, is normally present in low concentrations (< 1.0 mg/L). In groundwatees,
however, particularly those in contact with iron bearing minerals, higher concentrations (up
to 10 mg/L or more) are found.
Elevated concentrations of iron can be problematic in drinking water for three principal
reasons:
• staining of laundry and other items can occur due to the precipitation of hydrated ferric
oxide
• colloidal suspensions of the precipitate can also mean that the water looks unpleasant
• iron can also impart an unpleasant taste to the water which some people can detect at
levels above 1.0 mg/L
In India, there is a limit on iron in water that is to be used for drinking without treatment of 0.3
mg/L and in raw water that is to be used for drinking after conventional treatment of 50 mg/L.
2. Phenanthroline Spectrophotometric Method
This method relies on the fact that iron, when converted to its ferrous state by reaction with
acidified hydroxylamine, forms a colour-stable complex with phenanthroline. The red
coloured complex formed can then be measured with a spectrophotometer at a wavelength
of 510 nm.
3. Experiment
Aim
a. To determine the concentration of total iron in a number of different samples by
spectrophotometry.
Method
a. Read the SAP for total iron determination
b. Collect a sample from each of the buckets marked A, B and C.
c. Determine the concentration of total iron in each sample according to the Standard
Analytical Procedure for Iron.
22. HP Training Module File: “ 43 How to Measure Total Iron .doc” Version: September 2000 Page 2
Observations & calculation
a. Fill in the table as you proceed with the method:
Sample Absorbance at 510 nm
0.1 mg/l Standard Solution
0.2 mg/l Standard Solution
0.3 mg/l Standard Solution
0.5 mg/l Standard Solution
1.0 mg/l Standard Solution
2.0 mg/l Standard Solution
3.0 mg/l Standard Solution
4.0 mg/l Standard Solution
A
B
C
b. Use the values of the absorbance for the standard solutions in the table to plot a
standard curve of iron versus absorbance.
c. Read the total iron concentrations of the three samples from the standard curve.
Report
The three samples all represent potential drinking waters each with different concentrations
of iron. On the basis of your results state whether these waters are suitable for use as
drinking water. Report as:
- Unsuitable for drinking water use
- Suitable for drinking water use after conventional treatment
- Suitable for drinking water use without treatment
23. HP Training Module File: “ 43 How to Measure Total Iron .doc” Version: September 2000 Page 3
24. HP Training Module File: “ 43 How to Measure Total Iron .doc” Version: September 2000 Page 4