Follow this guidelines for Do and Don't during Animal Handling, Giving injectables, Giving Euthanasia and dosing.

1. DO’s DON’Ts
Provide appropriate training
for personnel
Handle animals roughly or
forcefully
Use suitable equipment and
tools for handling
Neglect animal welfare
needs
Monitor animal health and
welfare regularly
Perform unnecessary
procedures
Provide appropriate housing
and environmental
enrichment
Expose animals to
hazardous conditions
Minimize stress and
discomfort during handling
procedures
Violate regulatory
requirements
Implement proper hygiene
Use animals for non-
scientific purposes
Provide good sanitation
practices.
Keep animals in
overcrowded or
unsanitary conditions
Follow institutional and
regulatory guidelines
according to CPCSEA and
IAEC.
Conduct research without
proper ethical review and
approval
DO’s DON’Ts
Ensure proper training for
personnel and use appropriate
anesthesia.
Administer incorrect dosages.
Follow approved protocols and
regulations according to CPCSEA
and IAEC
Administer medications without
proper training.
Calculate doses accurately based
on body weight or surface area.
Exceed recommended dosage
limits.
Monitor animals closely for
unwanted effects.
Administer medications to
unhealthy or stressed animals.
Record all dosing information
accurately in study records.
Use expired or compromised
medications.
Dispose of needles and syringes
properly after use.
Reuse needles or syringes.
Properly handle and store
medications to maintain their
integrity.
Administer medications via
incorrect routes (e.g., intravenous
instead of intraperitoneal).
Minimize stress and discomfort
during dosing procedures.
Neglect proper training and
education on dosing techniques.
Communicate effectively with
the research team and veterinary
staff.
Neglect to obtain necessary
ethical approvals for animal
research.
DO’s DON’Ts
Use appropriate anesthesia
or sedation techniques
Don't perform blood
collection without proper
training
Follow aseptic techniques for
sample collection
Don't reuse needles or
syringes
Select appropriate blood
collection sites on the basis
of amount of blood needed.
Don't perform excessive
or unnecessary blood
collection
Use proper restraint methods
for the animal
Don't stress or harm the
animal during collection
Monitor vital signs during and
after collection
Don't collect blood from
unhealthy or debilitated
animals
Label blood samples
accurately and clearly
Don't mix up or mislabel
blood samples
Handle and store blood
samples properly
Don't delay processing or
analysis of blood samples
Dispose of sharps and
biohazardous waste safely
Don't expose yourself or
others to bloodborne
pathogens
DO’s DON’Ts
Ensure proper training for
personnel
Don't perform euthanasia
without appropriate training
Follow institutional protocols
and guidelines according to
CPCSEA and IAEC.
Don't delay euthanasia when
it's necessary
Use appropriate anesthetic
agents and methods
Don't use euthanasia as a
means of convenience
Handle the animal gently
Don't perform euthanasia in
front of other animals
Confirm loss of vital signs
before disposal
Don't neglect to monitor the
animal's response to the
procedure
Provide support and counseling
for staff involved
Don't ignore emotional impact
on staff members
DURING GIVING EUTHANASIA
DURING BLOOD COLLECTION
DURING ANIMAL DOSING
DURING ANIMAL HANDLING
DO'S AND DON’Ts