Escort Service Call Girls In Sarita Vihar,, 99530°56974 Delhi NCR
Â
Diagnosis of Anemia
1. Diagnosis of a case of
Anemia
Dr. Md Shahid Iqubal
Deptt Of Medicine, Nmch
2. Definition of anaemia
⢠Anaemia is a condition in which the number of
red blood cells or their oxygen-carrying capacity
is insufficient to meet physiologic needs, which
vary by age, sex, altitude, smoking, and
pregnancy status.
⢠The WHO criteria for anemia as hemoglobin (Hb)
levels <12.0 g/dL in nonpregnant women and
<13.0 g/dL in men(in adults).
⢠Anemia in pregnancy is defined as a hemoglobin
concentration of less than 11 g/dL .
3. DATA REPORTS
⢠It is a major public health problem
⢠Estimated prevalence of anemia in india is 42% in
women 15â59 years, 30% in men 15â59 years,
and 45% in adults >60 years.
⢠According to World Health Organization,
prevalence of anaemia among pregnant women
in developed countries is about 14%, whereas it is
still as high as 51% in the developing world
⢠The prevalence of anemia is 98% among the
pregnant females in this region of rural India.
4. Grading of anaemia
Grade of anemia Hb concentration
Mild Hb from lower limit of normal to 10g/dl
Moderate 10.0 to 7.0 g/dl
Severe <7.0 g/dl
6. Morphological classification of anemia
Microcytic anemia
(MCV<80fl)
Macrocytic anemia
(MCV>100fl)
Normocytic anemia
(MCV 80-100 fl)
Iron deficiency anemia Megaloblastic anemia Reticulocyte production
normal
Thalassemia Nonmegaloblastic anemia ⢠Recent blood loss
⢠hemolytic anemia
Sideroblastic anemia ⢠liver disease Reticulocyte production
deficient
Anemia of chronic disease ⢠hemolytic anemia ⢠Anemia of chronic
disease
⢠alcoholism ⢠aplastic anemia
⢠myelodysplastic
syndrome
⢠chronic kidney disease
⢠hypothyroidism ⢠hypothyroidism
7. Clinical presentation of anemia
ďąSymptoms
⢠Fatigue
⢠Malaise
⢠Dyspnea and palpitation
⢠Syncope
⢠Dizziness
⢠Menorrhagia
⢠Loss of appetite
8. Diagnosis of anemia
ďąHistory
⢠Diet history-vegeterian or nonvegeterian
⢠h/o-chronic blood loss(menorrhagia,hemorrhoids)
⢠h/o-drugs like anticancerous agents,
chloramphenicol,gold,penicillamine(aplastic anemia)
Dapsone,quinine(hemolytic anemia)
⢠Family history of anemia(thalassemia,sickle cell
anemia)
⢠h/o alcohol addiction
⢠h/o-renal disease,rheumatologic disease
⢠History of systemic symptoms like fever ,weight
loss,night sweats.
⢠Obstetric and menstrual history
10. Investigation
⢠CBC
⢠Reticulocyte count
⢠ESR
⢠Peripheral blood smear
⢠LFT(recent viral hepatitis)
⢠RFT
⢠Iron profile
⢠LDH,uric acid
⢠Vitamin B12 and folic acid level
⢠Bone marrow examination
⢠Hg electrophoresis
⢠Flowcytometry
⢠Direct and indirect coombs test
⢠Screening test for hep A,B and C
13. Iron deficiency anemia
ďąCause-blood loss(menses,GI blood loss)
celiac disease,h.pylori infection
⢠History of pica(consumption of substances
such as ice, starch, or clay)
⢠koilonychia (âspoon nailâ), and
glossitis(Plummer-Vinson's
syndrome) seen in severe iron
deficiency anemia
14. Investigations
⢠CBC and red cell indices-âHb, âMCV, âMCH, âMCHC
⢠Reticulocyte count-normal or decreased
⢠PBS âMicrocytic and hypochromic
anisocytosis and poikilocytosis
⢠Iron profile-ferritin decreased (<10ng/ml in women and <20ng/ml
in men)
serum iron â,TIBCâ
⢠BM biopsy-absent or â staining
for iron.
Severe iron deficiency anemia
(Normal serum iron-50-150Âľ/dL
Normal TIBC-300-360Âľ/dL)
15. Thalassemia
⢠The thalassemia syndromes are inherited
disorders of ι- or β-globin biosynthesis.
⢠Alpha and beta thalassemia
Alpha beta thalassemia
Silent carrier Minor
trait intermedia
Hemoglobin H Major(cooleyâs anemia)
hydrops fetalis
16. Diagnosis of Thalassemia
⢠The diagnosis of β Thalassemia major made
during childhood.
⢠Minor and intermedia remains asymptomatic
⢠On examination â jaundice, hepatosplenomegaly,
ďąInvestigation â
⢠CBC- âMCV, âMCH
⢠Reticulocytosis
⢠Peripheral smear-microcytic hypochromic RBCs
with poikilocytosis ,target cells
17. Peripheral blood smear of thalassemia
Microcytic and hypochromic
resembling severe iron-deficiency
anemia. Many elliptical and
teardrop-shaped red blood cells are
noted.
Target cells have a bullâs-eye
appearance
18. Diagnosis of Thalassemia contdâŚ.
⢠Hb electrophoresis is diagnostic for β â
thalassemia
⢠HbF(ι2γ2), HbA2 (ι2δ2) or both increased.
⢠In ι thalassemia trait HbA2 and HbF levels are
normal.
(Normal HbF<1%,HbA2-2.5-3.5%)
⢠Hb H disease have increased β tetramers.
19. Differential diagnosis of Microcytic anemia
Tests Iron Deficiency Inflammation Thalassemia Sideroblastic
Anemia
smear Micro/hypo Normal
Micro/hypo
Micro/hypo
with targeting
variable
Serum
iron(Îźg/dL)
<30 <50 Normal to high Normal to high
TIBC(Îźg/dL) >360 <300 normal normal
Percent
saturation
<10 10-20 30-80 30-80
Ferritin(Îźg/L) <15 30-200 50-300 50-300
Hemoglobin
pattern on
electrophoresis
Normal Normal Abnormal with
β thalassemia;
Normal
21. Megaloblastic anemia
⢠Megaloblastic anemia is a term used to describe
disorders of impaired DNA synthesis in hematopoietic
cells but affects all proliferating cells.
⢠Due to folic acid or vitamin B12 deficiency
ďąDiagnosis-
⢠In addition to sx of anemia peripheral neuropathy,
paresthesias,
Seizures and dementia may found due to vit b12
deficiency
⢠On examination- Jaundice or splenomegaly
⢠Decreased vibratory and positional sense, ataxia,
22. Investigations of megaloblastic anemia
⢠Increased MCV & MCH,normal MCHC
⢠Low RBC ,WBC,and platelets
⢠Peripheral smear-oval macrocytes,anisocytosis,poikilocytosis,
Hypersegmented neutrophils
⢠LDH and indirect bilirubin are elevated
⢠Raised urine urobilinogen
⢠Serum vitamin B12 ,or folate or both decresed
(Normal serum vit B12levels 160â200 ng/L and normal serum folate
2 -15 Îźg/L)
⢠Serum methylmalonic acid (MMA) and homocysteine (HC) are
elevated in vitamin B12 deficiency; and only HC is elevated in folate
deficiency.
⢠Detecting antibodies to intrinsic factor is specific for the diagnosis
of PA.
23. Peripheral smear of megaloblatic anemia
Severe megaloblastic anemia Macro-ovalocytes
25. Aplastic anemia
⢠Aplastic anemia is pancytopenia with bone
marrow hypocellularity.
⢠Inherited-fanconi anemia,dyskeratosis
congenita
⢠Acquired-radiation,drugs like cytotoxic
drugs,benzene,chloramphenicol,NSAIDS,sulfo
namides,gold,mercury,hydantoin,parvo
virus,hepatitis,EB virus,HIV-1
26. Diagnosis of aplastic anemia
⢠History-of bleeding,easy bruising,nose bleeds,heavy
menstrual flow
⢠Family history of hematologic ds
⢠Examination-petechiae and ecchymoses
⢠Lymphadenopathy and splenomegaly are highly atypical of
aplastic anemia. Cafe au lait spots and short stature
suggest Fanconi anemia
⢠MCV-increased,reticulocytes are absent or few
⢠Peripheral smear-shows large erythrocytes and a paucity of
platelets and granulocytes.
⢠Bone Marrow-only red cells,residual lymphocytes,mainly
fat
⢠Chromosome studies of bone marrow cells for MDS
⢠Flow cytometry to rule out PNH.
27. Anemia of chronic kidney disease
⢠Primarily due to decreased endogenous EPO
production
⢠Other causes are-Diminished red blood cell
survival, Bleeding diathesis
⢠Iron deficiency
⢠Hyperparathyroidism/bone marrow fibrosis
⢠Chronic inflammation
⢠Folate or vitamin B12 deficiency
⢠Comorbid conditions: hypo-/hyperthyroidism,
pregnancy,
31. Diagnosis of hemolytic anemia
⢠General examination- Jaundice, pallor
⢠Other physical findings Splenomegaly; bossing of skull
⢠Hemoglobin level From normal to severely reduced
⢠MCV, MCH Usually increased
⢠Reticulocytes Increased
⢠Bilirubin Increased (mostly unconjugated)
⢠LDH Increased
⢠Haptoglobin Reduced to absent
⢠direct Coombs test [DAT] is an indicator of the presence of
antibodies attached to RBC.
⢠The indirect Coombs test indicates the presence of free antibody in
the plasma.
⢠A peripheral blood smear-in Intravascular hemolysis may show red
cell fragmentation (i.e., schistocytes,helmet cells)
32. Hereditary Spherocytosis
⢠Autosomal dominant
⢠Jaundice,splenomegaly,gall stones
⢠MCHC increased
⢠Peripheral smear-normocytic,spherocytes
⢠Screening test-osmotic fragility test
⢠Definitive dx by molecular studies of gene
33. Sickle cell anemia
⢠Due to point mutation in 6th place of beta
chain ,glutamic acidâvaline
⢠On deoxygenation sickle cells are formed
ďąClinical presentation
⢠Vaso-occlusive crisis-acute chest
syndrome,pain crisis
⢠Aplastic crisis-by parvovirus B19
⢠Hemolytic crisis-gall stones
⢠infections
35. Diagnostic testing of sickle cell anemia
Sickling test
Sickling and solubility test âscreening test of sickle cell anemia
36. Diagnostic testing of sickle cell anemia
⢠HPLC- Hb analysis by high performance liquid chromatography
homozygoteheterozygote
37. Glucose-6-phosphate dehydrogenase deficiency
⢠X-linked disorder
⢠Reduced activity of G6PD
⢠Inability to remove H2O2
⢠Accumulated H2O2 leads to oxidation of
hemoglobin with precipitation of globin chains
⢠May Present as neonatal jaundice
⢠Incresed bilirubin and Hemoglobinuria.
38. Peripheral smear of G6PD
⢠On Peripheral smear-bite cells and heinz
bodies (precipitated Hb within RBCs).
39. Autoimmune Hemolytic Anemia
ďą Warm antibody AIHA-by an IgG autoantibody
⢠Lymphoma,CLL,collagen vascular ds
ďą Cold antibody AIHA-by an IgM autoantibody
⢠Seen in cold agglutinin ds,mycoplasma EB virus
ďą Diagnosis-
⢠reticulocytosis,elevated LDH, and indirect
hyperbilirubinemia.
⢠Peripheral blood smear may show spherocytes, occasional
fragmented RBCs,
⢠Positive DAT(direct coombs test)
⢠Warm AIHA: IgG +and/or C3+
⢠Cold AIHA: IgG-and C3+
40. Take home message
⢠Anemia is one of the important cause of morbidity and
mortality in women.
⢠Iron deficiency anemia is the most common type of anemia
⢠Iron deficiency in adult male means GI blood loss until
proven otherwise.
⢠Hb electrophoresis is diagnostic for thalassemia
⢠Megaloblastic anemia may present as pancytopenia
⢠Reticulocytosis present in hemolytic anemia
⢠Microcytic hypochromic âiron deficiency anemia
⢠Macrocytic-megaloblastic anemia
⢠Normocytic normochromic-hemolytic anemia