This curriculum vitae summarizes the qualifications and experience of David John Jackson. He has over 26 years of experience as a Senior Research Scientist in transfusion medicine, working with the UK Transplant Service and International Blood Group Reference Laboratory. His experience includes research in areas such as HLA typing, virology testing, cell culture, monoclonal antibody production, platelet immunology, and diagnostic development. He holds a Professional Doctorate in Biomedical Sciences and has published numerous research papers in peer-reviewed journals.
Despite greatly improved understanding of endothelial heterogeneity, the number of molecules discriminating human arterial and venous endothelium remains limited. Indeed, there have been few reports validating markers proposed in animal model studies in freshly isolated human tissues. We report here the global characterization of freshly isolated human umbilical arterial and venous endothelial cell (HUAECs and HUVECs) plasma membrane proteins using an experimentally validated label-free quantitative LC-MS/MS platform.
Patologie digestive, extradigestive e MicrobiotaASMaD
Presentazione a cura del Professor Giovanni Barbara - M.A.S.T.E.R. ECM in Gastroenterologia: Focus on: Microbiota e dintorni - Fondazione Santa Lucia - Roma
By identifying key cells and cytokines that play roles in the development of diseases like psoriasis and atopic dermatitis, we have been able to target very specific parts of the immune system and thereby treat these diseases more effectively and more safely. Following the same methodology, we hope to identify key targets that allow us to do the same for alopecia areata.
Despite greatly improved understanding of endothelial heterogeneity, the number of molecules discriminating human arterial and venous endothelium remains limited. Indeed, there have been few reports validating markers proposed in animal model studies in freshly isolated human tissues. We report here the global characterization of freshly isolated human umbilical arterial and venous endothelial cell (HUAECs and HUVECs) plasma membrane proteins using an experimentally validated label-free quantitative LC-MS/MS platform.
Patologie digestive, extradigestive e MicrobiotaASMaD
Presentazione a cura del Professor Giovanni Barbara - M.A.S.T.E.R. ECM in Gastroenterologia: Focus on: Microbiota e dintorni - Fondazione Santa Lucia - Roma
By identifying key cells and cytokines that play roles in the development of diseases like psoriasis and atopic dermatitis, we have been able to target very specific parts of the immune system and thereby treat these diseases more effectively and more safely. Following the same methodology, we hope to identify key targets that allow us to do the same for alopecia areata.
Identification of genetic regions in the yuk operon of Bacillus subtilis that are differentially required for secretion of YukE, a homolog to the virulence factor, ESXA in Mycobacterium tuberculosis
Identification of genetic regions in the yuk operon of Bacillus subtilis that are differentially required for secretion of YukE, a homolog to the virulence factor, ESXA in Mycobacterium tuberculosis
Nesta apresentação o Engenheiro Sanitarista Hiram Sartori proporciona aos interessados uma introdução bastante completa ao tema da compostagem.
Os interessados podem conhecer mais sobre o tema e aprender como proceder com a compostagem.
Para mais informações, acesse: http://www.hiramsartori.com.br
I am a science graduate with 5 years and 7 months of total experience. 1 year in calling profile with Wipro BPO, 4 years and 10 months into HR Outsourcing with AonHewitt and 2 Years 4 Months with SoftwareONE into Software Licensing and Master Data Management.
Currently Working as a Service Delivery Leader with SoftwareONE.
Chemokine (C-X-C) ligand 1 (CXCL1) protein expression is increased in aggress...Enrique Moreno Gonzalez
Chemokines, including chemokine (C-X-C motif) ligand 1 (CXCL1), may regulate tumor epithelial-stromal interactions that facilitate tumor growth and invasion. Studies have linked CXCL1 expression to gastric, colon and skin cancers, but limited studies to date have described CXCL1 protein expression in human bladder cancer (BCa) tissues.
dkNET Webinar "The Multi-Omic Response to Exercise Training Across Rat Tissue...dkNET
Presenter: Malene Lindholm, PhD, Instructor, Department of Medicine, Stanford University
Abstract
The Molecular Transducers of Physical Activity Consortium (MoTrPAC) aims to map the molecular responses to exercise and training to elucidate how exercise improves health and prevents disease. The first MoTrPAC data provides an extensive temporal map of the dynamic multi-omic response to endurance training across multiple rat tissues. All results can be viewed, interrogated, and downloaded in a user-friendly, publicly accessible data portal (https://motrpac-data.org). The MoTrPAC data compendium includes transcriptomics, proteomics, metabolomics, phosphoproteomics, acetylproteomics, ubiquitylproteomics, DNA methylation, chromatin accessibility, and multiplexed immunoassay data. This compilation constitutes of 211 datasets across 19 tissues, 25 molecular assays, and 4 training time points in adult male and female rats. Over 35,000 analytes were found to be differentially regulated in response to endurance training, with many displaying sexual dimorphism. We observed a male-specific recruitment of immune cells to adipose tissues and an anticorrelated transcriptional response in the adrenal gland related to the stress response. Temporal multi-omic and multi-tissue integration demonstrated similar temporal responses in the heart and skeletal muscle, reflecting a concerted adaptation of mitochondrial biogenesis and metabolism. Integrative multi-omic network analysis revealed connections between the heat shock-mediated stress response and mitochondrial biogenesis. Training increased phospholipids and decreased triacylglycerols in the liver, and there were extensive changes to mitochondrial protein acetylation. Many changes were relevant for human health conditions, such as non-alcoholic fatty liver disease, inflammatory bowel disease, cardiovascular wellness, and tissue damage and repair. Altogether, this MoTrPAC resource provides an unprecedented view of the effects of exercise across an organism, revealing mechanistic details of how exercise impacts mammalian health. The MoTrPAC data hub is the primary online resource to disseminate this large-scale multi-omics data.
The top 3 questions that the MoTrPAC resource can answer:
1. What is the multi-omic response to endurance exercise across different tissues?
2. What are the top signaling pathways affected in response to exercise and do they differ between males and females?
3. How can the MoTrPAC data hub be utilized to interrogate all the MoTrPAC findings?
Upcoming webinars schedule: https://dknet.org/about/webinar
1. C U R R I C U L U M V I T A E
DAVID JOHN JACKSON
5 Shaymoor Lane
Pilning
Bristol
South Gloucestershire
BS35 4JR
Telephone: 01454 501412
Mobile: 07752 844932
Email: joydave66@googlemail.com
PROFILE
I am an experienced Senior Research Scientist with over 26 years’ experience in
the UK Transplant Service and the International Blood Group Reference Laboratory,
NHS Blood Transplant in association with Bristol University. I have spent 23 years in
research and 3 years in a diagnostic laboratory working under containment level 3
laboratory conditions.
EXPERIENCE
HLA serological typing of human cells
Virology testing of hazardous human blood samples
Animal and human cell culture
Animal husbandry
Production of monoclonal anti-D antibodies by EBV transformation of human
immunised white cells
Production of human monoclonal antibodies using a SCID mouse model
Development of peptide therapy to ameliorate human alloantibody responses
FACS analysis
Light and fluorescent microscopy
Confocal microscopy
Development of assays using radioactive Isotopes
Platelet immunology
Diagnostic development of vCJD assays
Experience of working in a CL3 laboratory
Production of human recombinant proteins
Identification of vCJD in samples of human eye and spleen project
Molecular Biology
Collaboration with medics, nurses and colleagues to identify potential patients
for a NAIT research project
2. Talking with patients to explain research projects
National and International oral presentations of research work
Research papers (see below)
CAREER SUMMARY
20012- International Blood Group Reference Laboratory
Senior Clinical Scientist
1990-2012 International Blood Group Reference Laboratory
Research Scientist
1989-1990 UK Transplant Service
Scientific Officer
EDUCATION/QUALIFICATIONS
2006-2012 Professional Doctorate in Biomedical Sciences (PhD)
1991-1995 MPhil (Immunology)
1985-1989 BSc(Hons) Applied Biological Sciences
1982-1985 A Levels Chemistry, Biology, Physics
1977-1982 O Levels English, Maths, Biology, Physics, Chemistry,
History, Geography, Technical Drawing, Art
PROFESSIONAL MEMBERSHIP
Health and Care Professions Council(HCPC) –CS02810
British Blood Transfusion Service(BBTS)
PERSONAL
Date of Birth: 3rd February 1966
Married
British
NI no. NH792468D
3. ACTIVITIES/INTERESTS
SPORT: Cycling, running, weight training
OTHER: Photography
VOLUNTARY WORK:
Youth group leader to under privileged children.
Department head of the Sunday School in a large inner city church.
Department head of a team of 40 people responsible for the Hospitality in a large city
church of 400+ people.
Helping to distribute food, drink and clothing to the homeless in Bristol.
Facilitating a team of leaders responsible for the wellbeing and welfare of 40 adults.
Science practical demonstrations and talks to 12-18 year olds.
4. PUBLICATIONS
Human platelet antigen (HPA)-1a peptides do not reliably suppress anti-HPA-
1a responses using a humanized severe combined immunodeficiency (SCID)
mouse model.
Jackson DJ1, Eastlake JL, Kumpel BM.
Clin Exp Immunol 2014 Apr;176(1):23-36.
Ultrastructural localization of glycoprotein IIIa (GPIIIa, beta 3 integrin) on
placental syncytiotrophoblast microvilli: implications for platelet
alloimmunization during pregnancy.
Kumpel BM, Sibley K, Jackson DJ, White G, Soothill PW.
Transfusion. 2008 Oct;48(10):2077-86
Reactivity of T cells from women with antibodies to the human platelet antigen
(HPA)-1a to peptides encompassing the HPA-1 polymorphism.
Jackson DJ1, Murphy MF, Soothill PW, Lucas GF, Elson CJ, Kumpel BM.
Clin Exp Immunol 2005 Oct;142(1):92-102.
Flow cytometric characterisation of cells of differing densities isolated from
human term placentae and enrichment of villous trophoblast cells.
Manoussaka MS1, Jackson DJ, Lock RJ, Sooranna SR, Kumpel BM
Placenta 2005 Apr;26(4):308-18
Reduction of human anti-tetanus toxoid antibody in hu-PBL-SCID mice by
immunodominant peptides of tetanus toxoid.
Jackson DJ1, Elson CJ, Kumpel BM.
Clin Exp Immunol 2004 Aug;137(2):245-52.
Section 1C: Assessment of the functional activity and IgG Fc receptor
utilisation of 64 IgG Rh monoclonal antibodies. Coordinator's report.
Kumpel BM1, Beliard R, Brossard Y, Edelman L, de Haas M, Jackson DJ, Kooyman
P, Ligthart PC, Monchâtre E, Overbeeke MA, Puillandre P, de Romeuf C,Wilkes AM.
Transfus Clin Biol. 2002 Jan;9(1):45-53.
Comparison of flow cytometric assays with isotopic assays of (51)chromium-
labeled cells for estimation of red cell clearance or survival in vivo.
5. Kumpel BM1, Austin EB, Lee D, Jackson DJ, Judson PA, Chapman GE.
Transfusion 2000 Feb;40(2):228-39.
Optimisation of human anti-tetanus toxoid antibody responses and location of
human cells in SCID mice transplanted with human peripheral blood
leucocytes.
Jackson DJ1, Kumpel BM.
Human Antibodies 1997;8(4):181-8.
Characterization and functional activity of human Rh monoclonal antibodies.
Kumpel BM1, Jackson DJ.
Transfus Clin Biol 1996;3(6):453-8.
Human leucocyte antigen (HLA) and malaria morbidity in a Gambian
community.
Bennett S1, Allen SJ, Olerup O, Jackson DJ, Wheeler JG, Rowe PA, Riley
EM, Greenwood BM.
Trans R Soc Trop Med Hyg. 1993 May-Jun;87(3):286-7
Specific anti-A responses of SCID mice populated with human lymphoid cells
from peripheral blood, umbilical cord, bone marrow and spleen after
immunization with group A erythrocytes [corrected].
Kumpel BM1, Poole GD, Jackson DJ.
Immunol Lett 1992 Jul;33(2):163-8.