CYTOOchips Arena provide users with cell confinement areas of different sizes compatible with observations under a wide range of magnifications (5x to 63x). The chips are particularly useful for monitoring highly motile cells as well as cell lineage and cell fate studies. Arenas can also be used for standardized cell colony growth.

1. chips Arena ™
Don’t let your cells escape!

2. chips Arena CYTOOchip layout
Don’t let your cells escape!
CYTOOchips Arena provide users with cell confinement adhesive areas
of different sizes compatible with observations under a wide range of
magnifications (5x to 63x). The chips are particularly useful for monitoring
highly motile cells as well as for cell proliferation, cell lineage and cell
fate studies. The chips can also be used for standardized cell colony growth.
Micropatterns are available coated in fibronectin (fluorescent or not) or as
ready-to-coat. Protocols are available to coat proteins such as collagen, HeLa cells confined on 500µm Arena discs imaged
Poly-L-Lysine, laminin or Matrigel®. under 5x magnification
Benefits and key features Applications
• Cell confinement areas (adhesive discs of 80, 140, 225, 500 • Time-lapse measurements
and 1000 μm in diameter) corresponding to magnifications of 63x to 5x • Observation of highly motile cells
• No cell loss during time lapse experiments • Monitoring of cell fate
• Easy cell tracking • Precise cell proliferation studies
• Easy acquisition automation on multiple areas thanks to the integrated • Standardized cell groups The discs of different diameters (from 500 down to 80 µm) are organized over the chip in 4 identical quadrants. Each quadrant
cell localization grid • Cell colony growth is again divided into 4 zones and each disc diameter width is arrayed over 3x3 blocks. The blocks separating the 4 quadrants
• Designed to be compatible with 1 or 4 well CYTOOchambers • Precise cell cycle measurements feature the discs of 1000 µm (one disc per block).
Example: Monitoring cell fate upon serum starvation CYTOOchambers™ for Live Cell Imaging
Fibronectin, Discs 500 µm, RPE1 cells, 10x CYTOOchambers are easy-to-use devices designed for time-lapse experiments on CYTOOchips 20x20. Available in 1- or 4-well
formats, CYTOOchambers have a standard foortprint and fit into all 35 mm microscope stage adaptors.
The CYTOOchip Arena has been designed to be compatible with both 1 well and 4 well CYTOOchambers.
2.0 h 12.0 h 36.0 h 50.0 h
RPE1 cells were monitored in 10× time-lapse phase-contrast microscopy to measure cell division time with respect to time of
starvation and observe daughter cell fates. Colored dots are used as visual marks to follow individual cell fates: (i) the blue
cell’s daughters were starved in late G1 and divided again; (ii) the red cell’s daughters were starved in early G1 and didn’t
divide again; (iii) the green cell’s daughters adopted asymmetric behavior: one divided again and not the other.
From Pitaval et al. J Cell Biol. 2010, 191(2): 303-12.
Arena discs imaged at 5x
4 well CYTOOchamber
1 well CYTOOchamber
80 µm 225 µm 500 µm 1000 µm

3. Product specifications
Substrate High quality low fluorescence glass (170 μm thick, 1.5)
Chip size 20 x 20 mm² (CYTOOchip 20x20 format)
Patterned area 16.9 x 16.9 mm
Micropattern geometries Discs from 80 to 1000 μm of diameter
Disc diameter, μm 80 140 225 500 1000
Corresponding objective (one disc in the FoV) 63x 40x 20x 10x 5x
Pattern area, μm² 5,000 15,400 39,800 196,000 785,000
Micropatterns per block 36 25 16 4 1
Number of micropatterns per chip 1,296 900 576 144 25
Pitch between patterns, μm 217 260 325 650 1,300
Gap between patterns, µm 137 120 100 150 300
Adhesion protein Fibronectin (FN), FN650* or Ready-to-coat (A)**
Adhesive separation bands between blocks No
Cell localization grid Yes
Error-free identification of patterned side Yes
Chip identification letter Yes
Compatibility with CYTOOchambers 35 mm 1, 2 or 4 wells
Packaging By 6; in a blister pack sealed in aluminium bag under inert gas
* Fluorescent micropatterns (FN labeled with fluorophore with exc.
=650 µm): useful for easier pattern identification or
autofocusing as well as for automated image analysis over multiple
patterns.
Pitch Gap
** Activated CYTOOchips for adsorption of the protein of your choice
(Collagen, Laminin, Poly-Lysine, Matrigel®, specific antibodies etc.).
Protein may be fluorescently labeled. Contact us for recommended
coating protocols and specific needs.
Example of 500 µm discs (for 10x objective)
Ordering information
Cat. No. Product Description
10-020-10-06 CYTOOchips Arena FN Glass 20x20 mm; 170µm; Gridded; Disc patterns; Diam. 80, 140,
225, 500 and 1000 µm; Fibronectin; set of 6
10-020-13-06 CYTOOchips Arena FN650 Glass 20x20 mm; 170µm; Gridded; Disc patterns; Diam. 80, 140,
225, 500 and 1000 µm; Fibronectin labeled 650 nm; set of 6
10-020-00-06 CYTOOchips Arena A Glass 20x20 mm; 170µm; Gridded; Disc patterns; Diam. 80, 140,
225, 500 and 1000 µm; Activated (ready to coat); set of 6
30-010 CYTOOchamber 1 well Ext. diameter 35 mm; with lid; 1 well; Max. imaging area
16.5 x 16.5 mm2
30-012 CYTOOchamber 2 wells Ext. diameter 35 mm; with lid; 2 wells; Max. imaging area 16.5
x 7.5 mm2
30-011 CYTOOchamber 4 wells Ext. diameter 35 mm; with lid; 4 wells; Max. imaging area
7.5 x 7.5 mm2 per well
30-020 Microscope adaptor plate With clips; accept up to 6 CYTOOchambers
CYTOO S.A.
For inquiries please contact us at www.cytoo.com/contact 7, parvis Louis Néel
BHT 52, BP50
38040 Grenoble cedex 9 - FRANCE
Further Reading Tel. +33(0) 438 88 47 05
Find here a list of relevant publications: www.cytoo.com/publications CYTOO, Inc
Harvard Square
One Mifflin Place
Suite 400
IP Rights Cambridge, MA 02138
Tel. +1 617 674 7711
Adhesive micropattern products provided by CYTOO are covered by European and US Patents and patents pending as well as their foreign equivalents.
These products may be covered by one or more Limited Use Label Licenses. The purchase of this product conveys to the buyer the non-transferable www.cytoo.com - info@cytoo.com
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right to use the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise © 2009 CYTOO S.A.
CYTOO™, CYTOOchips™, CYTOOplates™,
transfer this product to a third party or otherwise use this product for Commercial Purposes. By the use of these products, you accept the terms and CYTOOchambers™ and Reference Cell™
conditions of all applicable Limited Use Label Licenses. For research use only. Not intended for any animal or human therapeutic or diagnostic use. are trademarks of CYTOO S.A.