InSphero produces standardized 3D tumor microtissues using their GravityPLUS platform which gently coerces cells to self-assemble into spheroids without scaffolds. These microtissues display key features of in vivo tumors, including cell-cell contacts and extracellular matrix production. They are available in 96-well plates ready for high-throughput screening and are more predictive of drug responses than traditional 2D cell cultures.

1. 3D tumor
microtissues
Oncology Products
» Provide an ideal basis for increasing your assay’s
predictive power
» Assay-ready, no cell culturing needed
» No scaffolds, so no unwanted artefacts
» Custom tumor microtissue production possible
(e.g. cocultures)
» Supplied in automation-friendly 96-well format

2. How are InSphero 3D tumor microtissues made?
InSphero employs the GravityPLUS platform to precision mass-fabricate standardized tumor micro-
tissues with a size variability of 10%. The GravityPLUS process gently coerces cells to assemble together
by gravity. This method completely avoids the use of polymeric scaffolds that could potentially interact
with drug compounds, assay reagents or cells.
What are 3D tumor microtissues?
Tumor microtissues are also known as multicellular tumor spheroids. These highly biomimetic 3D tumor
models display the gene expression patterns and the histomorphological, functional and microenviron-
mental features of in vivo human tumor tissue. 3D tumor microtissues are ideal for drug discovery and
far more predictive of clinical efficacy compared to 2D cultures. At last these valuable 3D models are
conveniently available “off-the-shelf” in large quantities, ready-for-use in high-throughput applications.
Biomimetic features
The GravityPLUS process results in the rapid formation of cell-cell contacts, cell-cell communication, cell
type-specific extracellular matrix and cell-matrix interaction. The morphology and functionality of the in
vivo tumor is clearly evident. In fact, unlike cells in 2D, InSphero tumor microtissues distinctly resemble
tissue from the inter-vascular regions of solid tumors.

3. One Dimension
ahead Advantages of InSphero Tumor Microtissues
More biomimetic: Compared to 2D monolayers, InSphero’s 3D microtissues can help to establish more
predictive in vitro drug testing, since phenomena such as multicellular resistance are clearly evident
No scaffolds: Polymeric scaffolds are not used which means freedom from concerns about unknown
and unwanted assay artefacts due to scaffold interactions with drug compounds, assay reagents or cells.
Assay-ready: InSphero tumor microtissues are available in industrial-scale quantities, in standard
96-well format, ready for screening or other HT applications.
Longer culture lifetime: Whereas 2D monolayer cultures offer only a very narrow time window for
gathering data this is extended many-fold with InSphero’s tumor microtissues.
Use your own cell lines: Complement InSphero’s off-the-shelf offer with your own cell lines in 3D
using InSphero’s custom production services.
© Luftbildfotograf - Fotolia
www.insphero.com

4. Product Overview Services Overview
Assay-ready tumor microtissues Also inquire about our custom micro–
Our growing list of assay-ready tumor microtissues (one microtissue per well in a standard 96-well plate) tissue-production services to upgrade
currently includes the products below : your cell line to 3D.
Cat. # Name Description Cell line
MT-01-001-01 hKi-CCL001MT Human kidney carcinoma microtissues A498
MT-01-002-01 hPr-CCL001MT Human prostate carcinoma microtissues DU145
MT-01-003-01 hCo-CCL001MT Human colorectal carcinoma microtissues HCT116
MT-01-004-01 hCr-CCL001MT Human colon carcinoma microtissues HT29
MT-01-005-01 hLi-CCL001MT Human liver carcinoma microtissues HepG2
MT-01-006-01 hCNS-CCL001MT Human CNS carcinoma microtissues SNB19
cDNA Libraries from cell lines in 2D, 3D microtissues and 3D-coculture microtissues
Model type 2D 3D homotypic 3D heterotypic
Cell line with NIH3T3
A498 ET-01-001-00 ET-01-001-01 ET-01-001-02
DU145 ET-01-002-00 ET-01-002-01 ET-01-002-02
HCT116 ET-01-003-00 ET-01-003-01 ET-01-003-02
InSphero AG
HT29 ET-01-004-00 ET-01-004-01 N/A Technoparkstrasse 1
HepG2 ET-01-005-00 ET-01-005-01 ET-01-005-02 CH-8005 Zurich
SNB19 ET-01-006-00 ET-01-006-01 ET-01-006-02
Switzerland
A549 ET-01-007-00 N/A ET-01-007-02
MCF7 ET-01-008-00 N/A ET-01-008-02 www.insphero.com
PC3 ET-01-009-00 N/A ET-01-009-02 Phone: +41-44-515049-0
Paraffin-embedded multi-microtissue sections e-mail: sales@insphero.com
Cat. # Name Array contents
ET-02-001 Multi-Tissue Array Tumor 1 A498, DU145, HCT116, HT29,
HepG2, SNB19