This is a presentation on connective tissues, which is important for histology and pathology in the preclinical years of medical school. It gives a great overview of what is necessary for success on STEP 1 and Level 1.

1. Connective Tissue Laboratory
Orientation
Sun-Kee Kim, Ph.D.
Department of Cell and Developmental Biology
University of Michigan
September 13, 2010

2. Loose Connective Tissue: #29 Small Intestine

3. Collagen fibers and fibroblasts
Fibroblasts
Collagen fibers

4. #29 – Intestinal Villi
Lamina propria

5. Lamina propria (Loose connective tissue)

6. #20 or #40 Trachea
Tracheal ring - cartilage

7. Plasma cells

8. Plasma cells
Eccentrically located nucleus
Cartwheel appearance of nucleus
Basophilic cytoplasm
Egg-shaped cell with clear outline

9. #26 lymph node
Look for macrophages in
this lightly stained area

10. Macrophages
Kidney bean shaped nucleus (indented)
Eosinophilic cytoplasm with bubbly
appearance
Ingested materials in the cytoplasm
Fuzzy cell border (outline)

11. Macrophage Plasma Cell

12. #152 Pharynx - fat (white) cells
-Unilocular adipose tissue
-displaced flattened nuclei

13. Slide H2 Brown Fat
-Multilocular
adipose tissue
-Spherical nuclei

14. #160 Mast cells

15. #160 Mast cell

16. #27 Lymph node stained with H & E
– Reticular connective tissue

17. #28 Lymph node stained with silver
– Reticular connective tissue

18. Dense irregular CT in the dermis
Skin #106 or #112
Dermis
Dermis

19. #33 Dermis of skin (aldehyde Fuchsin stain)
Dense irregular connective tissue with elastic fibers

20. Elastic Fibers in dense irregular CT - slide 33 (Verhoeff stain)

21. #250 Vagina (Dense irregular CT)
H & E Trichrome

22. Skeletal muscle
Dense regular CT (tendon)
Dense regular CT
#112 (or #106) Musculo-tendon junction

23. #34 Tendon (Dense regular connective tissue)
tendon
Skeletal muscle

24. #34 Tendon (X-section)

25. Slide #36, aorta, aldehyde fuchsin Slide 88, aorta. H & E
Elastic connective tissue