Compatibility of DMSO spotting buffers with SCHOTT Nexterion Epoxy-silane coated microarray slides. Effect of using different spotting pins and DMSO concentrations.
bio analytical method validation usfda guidlineschandu chatla
This document provides guidance from the US FDA on bioanalytical method validation. It discusses key principles such as method development, validation parameters for chromatographic and ligand binding assays, calibration curves, quality controls, selectivity, sensitivity, accuracy, precision, recovery, and stability. The guidance is intended to help sponsors validate analytical methods used in human and animal studies to quantify drug, metabolite, protein, and biomarker levels in biological matrices like blood, serum and tissue.
MỘT SỐ GIẢI PHÁP GÓP PHẦN NÂNG CAO NĂNG LỰC CẠNH TRANH CỦA NGÂN HÀNG THƯƠNG M...vietlod.com
Ngân hàng là một trong những lĩnh vực hết sức nhạy cảm và phải mở cửa gần như hoàn toàn theo các cam kết gia nhập tổ chức thương mại thế giới WTO, hệ thống ngân hàng Việt Nam được xếp vào diện các ngành chủ chốt, cần được tái cơ cấu nhằm nâng cao năng lực cạnh tranh. Để giành thế chủ động trong tiến trình hội nhập, Việt Nam cần xây dựng một hệ thống ngân hàng có uy tín, đủ năng cạnh tranh, hoạt động có hiệu quả cao, an toàn, có khả năng huy động tốt hơn các nguồn vốn trong xã hội và mở rộng đầu tư. Việc này đòi hỏi sự nổ lực nhiều mặt từ phía Chính phủ, Ngân hàng Nhà nước, và chính nội tại các ngân hàng thương mại.
http://nckh.vietlod.com/
The dissolution test is an important means of assuring the continuing performance of non-solution orally administered drug products. The development of a dissolution test procedure is briefly discussed in USP general information chapter In Vitro and In Vivo Evaluation of Dosage Forms 1088, whereas general information chapter Validation of Compendial Procedures 1225 gives limited validation information for dissolution testing. Neither of these two chapters provides a level of detail and focus sufficient for dissolution testing. In 2001, a Stimuli article provided an initial rationale and discussion of content for a new general information chapter. The new chapter, The Dissolution Procedure: Development and Validation 1092, was intended to supplement the information in 1088 and 1225 and provided step-by-step detail for development and validation as well as offering information on new technology and equipment. In 2006, the chapter became official with the Second Supplement to USP 29–NF 24 (2–4).
The General Chapters—Dosage Forms Expert Committee 2010–2015 placed the review and possible revision of The Dissolution Procedure: Development and Validation 1092 on its work plan for the 2010–2015 revision cycle (2011) .
Drug Regulations has prepared this presentation based on the proposed chapter.
bio analytical method validation usfda guidlineschandu chatla
This document provides guidance from the US FDA on bioanalytical method validation. It discusses key principles such as method development, validation parameters for chromatographic and ligand binding assays, calibration curves, quality controls, selectivity, sensitivity, accuracy, precision, recovery, and stability. The guidance is intended to help sponsors validate analytical methods used in human and animal studies to quantify drug, metabolite, protein, and biomarker levels in biological matrices like blood, serum and tissue.
MỘT SỐ GIẢI PHÁP GÓP PHẦN NÂNG CAO NĂNG LỰC CẠNH TRANH CỦA NGÂN HÀNG THƯƠNG M...vietlod.com
Ngân hàng là một trong những lĩnh vực hết sức nhạy cảm và phải mở cửa gần như hoàn toàn theo các cam kết gia nhập tổ chức thương mại thế giới WTO, hệ thống ngân hàng Việt Nam được xếp vào diện các ngành chủ chốt, cần được tái cơ cấu nhằm nâng cao năng lực cạnh tranh. Để giành thế chủ động trong tiến trình hội nhập, Việt Nam cần xây dựng một hệ thống ngân hàng có uy tín, đủ năng cạnh tranh, hoạt động có hiệu quả cao, an toàn, có khả năng huy động tốt hơn các nguồn vốn trong xã hội và mở rộng đầu tư. Việc này đòi hỏi sự nổ lực nhiều mặt từ phía Chính phủ, Ngân hàng Nhà nước, và chính nội tại các ngân hàng thương mại.
http://nckh.vietlod.com/
The dissolution test is an important means of assuring the continuing performance of non-solution orally administered drug products. The development of a dissolution test procedure is briefly discussed in USP general information chapter In Vitro and In Vivo Evaluation of Dosage Forms 1088, whereas general information chapter Validation of Compendial Procedures 1225 gives limited validation information for dissolution testing. Neither of these two chapters provides a level of detail and focus sufficient for dissolution testing. In 2001, a Stimuli article provided an initial rationale and discussion of content for a new general information chapter. The new chapter, The Dissolution Procedure: Development and Validation 1092, was intended to supplement the information in 1088 and 1225 and provided step-by-step detail for development and validation as well as offering information on new technology and equipment. In 2006, the chapter became official with the Second Supplement to USP 29–NF 24 (2–4).
The General Chapters—Dosage Forms Expert Committee 2010–2015 placed the review and possible revision of The Dissolution Procedure: Development and Validation 1092 on its work plan for the 2010–2015 revision cycle (2011) .
Drug Regulations has prepared this presentation based on the proposed chapter.
Formulation and evaluation of mouth dissolving film containing antiemetic dru...siddhant thakur
The final presentation on the research topic of "FORMULATION AND EVALUATION OF MOUTH DISSOLVING FILM CONTAINING ANTIEMETIC DRUG FOR THE TREATMENT OF MOTION SICKNESS"
This presentation includes the Introduction about MDFs, Literature reviews, Statement of the research problem, Objectives of the study, Plan of research work, Drug selections, Preformulation studies, Design expert 11, Preparation of MDFs and Evaluations, Optimization of formulations and final conclusion
This document describes the development and validation of two stability-indicating methods for the quantitative analysis of rosuvastatin (ROSU) in the presence of its degradation products: a high-performance liquid chromatography (HPLC) method and a high-performance thin layer chromatography (HPTLC) method. Both methods were found to be precise, accurate, specific and sensitive for the analysis of ROSU in raw materials and pharmaceutical formulations in the presence of degradation products. The methods were fully validated per ICH guidelines and successfully applied to analyze ROSU levels in commercial tablet formulations.
The feature of the new general chapter 621 is that a column packed with small particles can be used if column length and particle ratio (L/dp) is kept constant between the designated and modified column. This enables high speed analysis of USP methods more than ever.
In this study, a USP method was successfully transferred to an ultra-high speed method with the system suitability requirements met.
For more information, go to SSI.Shimadzu.com. Thanks for viewing.
Trajan Scientific and Medical collaborates with academic and industry partners to develop and deliver innovative solutions to impact human wellbeing. Focusing on developing and commercialising technologies that enable analytical systems to be more selective, sensitive and specific, to improve biological, environmental or food related measurements. Global operations with hubs in Europe, USA, Asia and Austrailia serve over 100 countries with highly specialised products used in scientific analysis and laboratory consumables and devices for healthcare applications. Trajan’s comprehensive range of technical capabilities include precision glass fabrication and surface treatments, chemical synthesis and separation solutions, materials knowledge and integrated solutions for samples integrity, precision machining and design engineering, photonics sensing and device technologies, microscopy products, as well as clinical collection devices and methods.
Filtration Strategies for Optimal Development and Purification of a FMD Virus...Merck Life Sciences
This document summarizes a collaboration between ME VAC and Merck to develop an optimal filtration process for the production and purification of a foot-and-mouth disease (FMD) vaccine. Key points include:
1) Merck's Cellvento® BHK-200 serum-free medium was used for growing BHK21 cells and replicating the FMD virus. Millipore Express® filters demonstrated good sterilizing-grade filtration capacity for the cell culture media.
2) For clarification, the Millistak+® HC C0HC depth filter showed high capacity and low turbidity, allowing efficient single-step clarification.
3) Pellicon® 2 cassettes
a simple multi-utility miniature device used as test tube to 3D cell culture ...guo jun chen
IMAPlate 5RC96 is patented and developed by NCL New Concept Lab GmbH in Switzerland. It is a multi-utility miniature analytical platform that is capable of MANUALLY performing up to 96 individual liquid transfer, analysis, reaction and assay simultaneously. The device is compatible with most 96-well plate readers for the measurement and spectral analysis. The IMAPlate 5RC96 can be used as 96-channel self-dosed manual pipette for tiny amount liquid transfer, as a 96-micro long path-length high sensitive cuvette array for UV-VIS-IR spectrum detection with a flexible sample volume of 1 - 25 ul and as a virtual 96-microwell plate for different assays. The IMAPlate has very broad applications in life sciences and diagnostics and fits for both manual operation and automated liquid handling workstation.
Many assays performed in multi-well plate can easily be adapted to the IMAPlate with increased sensitivity and cost saving, for example ELISA, cell adhesion assay, protein quantification and so on. Due to its unique feature, it can also be used for 3D cell culture to prepare micro-tissues and perform subsequent testing and measurement directed in the device. If needed, the micro-tissues or cells in the IMAPlate can easily be transferred to any 96-well plate and even can be spotted on microscope slide directly from the IMAPlate.
Particle Size Analysis for Homogenization Process Development HORIBA Particle
Emulsions and suspensions are commonly used in pharmaceutical, chemical and consumer products. The pharmaceutical industry, in particular, uses emulsions and suspensions to increase drug efficacy by controlling their particle size and size distribution. Among various available preparation methods, high-pressure homogenization is one of the widely employed processes in the field. This webinar discusses ways to develop a robust homogenization process for making pharmaceutical emulsions by evaluating droplet size distribution.
View recorded webinars:
http://bit.ly/particlewebinars
Rapid UHPLC Determination of Common Preservatives in Cosmetic Products v2zq
This document presents a fast UHPLC method for quantifying six common parabens (preservatives) in cosmetic products. The UHPLC method using a 1.9 μm column achieved a run time of 3.5 minutes, an over 80% reduction compared to the 19 minute run time using a conventional HPLC column. The UHPLC method also reduced solvent usage by over 90%. The method was shown to be linear, precise, and able to detect parabens at levels within EU and FDA regulations using samples of face lotion and body lotion.
New Software Methods Enhance Sedimentation Velocity Analysis of Protein Aggre...KBI Biopharma
1) New software methods have improved the resolution and sensitivity of sedimentation velocity analysis, making it useful for comparability testing, formulation development, and quality control.
2) A method developed by Peter Schuck increases resolution of multiple components and detects very minor components below 1%. It provides higher resolution than SEC columns.
3) Another new method allows obtaining conformation and mass information at extremely low concentrations below 1 microgram total protein.
1) A study was conducted to reverse engineer an oral suspension containing a poorly water soluble drug to match the dissolution profile of the reference listed drug (RLD) using quality by design principles.
2) Two multivariate design of experiments (DoEs) were used, along with live in-situ dissolution monitoring using fiber optic probes, to identify formulation factors influencing dissolution and reduce development time.
3) The approach resulted in a new formulation matching the RLD dissolution profile within 72 hours, much faster than traditional methods requiring costly and time-consuming HPLC analysis.
Filtration Strategies for Optimal Development and Purification of a FMD Virus...MilliporeSigma
This poster presentation outlines the different filtration strategies and performances in the upstream and downstream process to develop a scalable, cost-efficient and GMP-compliant Foot and Mouth Disease (FMD) vaccine production:
• Introduction to foot and mouth disease (FMD) and background on FMD vaccines
• Review of cell culture, clarification, and concentration/diafiltration steps for the production and purification of the FMD vaccine
• Suggested further actions based on data outlined in this poster
To learn more about this topic or collaborate with our technical experts, schedule an in-person or remote visit at our M Lab™ Collaboration Centers: www.emdmillipore.com/mlab
Effect of Detergent Concentration and Type when printing on Epoxy Coated NEXT...SCHOTT
This document summarizes the results of an experiment testing the effect of detergent concentration and type on signal intensity and spot morphology when printing oligonucleotides on NEXTERION® Slide E microarray slides. The experiment found that Triton X-100 at concentrations between 0.015-0.04% produced uniform spot intensity, shape, and diameter with low variability in spot sizes. Both detergent concentration and type influenced spot size and signal intensity, with lower concentrations producing less uniform spots and higher concentrations risking irregular spot morphology. The optimal detergent must be determined empirically based on the specific coating, probes, and printing method used.
Formulation and evaluation of mouth dissolving film containing antiemetic dru...siddhant thakur
The final presentation on the research topic of "FORMULATION AND EVALUATION OF MOUTH DISSOLVING FILM CONTAINING ANTIEMETIC DRUG FOR THE TREATMENT OF MOTION SICKNESS"
This presentation includes the Introduction about MDFs, Literature reviews, Statement of the research problem, Objectives of the study, Plan of research work, Drug selections, Preformulation studies, Design expert 11, Preparation of MDFs and Evaluations, Optimization of formulations and final conclusion
This document describes the development and validation of two stability-indicating methods for the quantitative analysis of rosuvastatin (ROSU) in the presence of its degradation products: a high-performance liquid chromatography (HPLC) method and a high-performance thin layer chromatography (HPTLC) method. Both methods were found to be precise, accurate, specific and sensitive for the analysis of ROSU in raw materials and pharmaceutical formulations in the presence of degradation products. The methods were fully validated per ICH guidelines and successfully applied to analyze ROSU levels in commercial tablet formulations.
The feature of the new general chapter 621 is that a column packed with small particles can be used if column length and particle ratio (L/dp) is kept constant between the designated and modified column. This enables high speed analysis of USP methods more than ever.
In this study, a USP method was successfully transferred to an ultra-high speed method with the system suitability requirements met.
For more information, go to SSI.Shimadzu.com. Thanks for viewing.
Trajan Scientific and Medical collaborates with academic and industry partners to develop and deliver innovative solutions to impact human wellbeing. Focusing on developing and commercialising technologies that enable analytical systems to be more selective, sensitive and specific, to improve biological, environmental or food related measurements. Global operations with hubs in Europe, USA, Asia and Austrailia serve over 100 countries with highly specialised products used in scientific analysis and laboratory consumables and devices for healthcare applications. Trajan’s comprehensive range of technical capabilities include precision glass fabrication and surface treatments, chemical synthesis and separation solutions, materials knowledge and integrated solutions for samples integrity, precision machining and design engineering, photonics sensing and device technologies, microscopy products, as well as clinical collection devices and methods.
Filtration Strategies for Optimal Development and Purification of a FMD Virus...Merck Life Sciences
This document summarizes a collaboration between ME VAC and Merck to develop an optimal filtration process for the production and purification of a foot-and-mouth disease (FMD) vaccine. Key points include:
1) Merck's Cellvento® BHK-200 serum-free medium was used for growing BHK21 cells and replicating the FMD virus. Millipore Express® filters demonstrated good sterilizing-grade filtration capacity for the cell culture media.
2) For clarification, the Millistak+® HC C0HC depth filter showed high capacity and low turbidity, allowing efficient single-step clarification.
3) Pellicon® 2 cassettes
a simple multi-utility miniature device used as test tube to 3D cell culture ...guo jun chen
IMAPlate 5RC96 is patented and developed by NCL New Concept Lab GmbH in Switzerland. It is a multi-utility miniature analytical platform that is capable of MANUALLY performing up to 96 individual liquid transfer, analysis, reaction and assay simultaneously. The device is compatible with most 96-well plate readers for the measurement and spectral analysis. The IMAPlate 5RC96 can be used as 96-channel self-dosed manual pipette for tiny amount liquid transfer, as a 96-micro long path-length high sensitive cuvette array for UV-VIS-IR spectrum detection with a flexible sample volume of 1 - 25 ul and as a virtual 96-microwell plate for different assays. The IMAPlate has very broad applications in life sciences and diagnostics and fits for both manual operation and automated liquid handling workstation.
Many assays performed in multi-well plate can easily be adapted to the IMAPlate with increased sensitivity and cost saving, for example ELISA, cell adhesion assay, protein quantification and so on. Due to its unique feature, it can also be used for 3D cell culture to prepare micro-tissues and perform subsequent testing and measurement directed in the device. If needed, the micro-tissues or cells in the IMAPlate can easily be transferred to any 96-well plate and even can be spotted on microscope slide directly from the IMAPlate.
Particle Size Analysis for Homogenization Process Development HORIBA Particle
Emulsions and suspensions are commonly used in pharmaceutical, chemical and consumer products. The pharmaceutical industry, in particular, uses emulsions and suspensions to increase drug efficacy by controlling their particle size and size distribution. Among various available preparation methods, high-pressure homogenization is one of the widely employed processes in the field. This webinar discusses ways to develop a robust homogenization process for making pharmaceutical emulsions by evaluating droplet size distribution.
View recorded webinars:
http://bit.ly/particlewebinars
Rapid UHPLC Determination of Common Preservatives in Cosmetic Products v2zq
This document presents a fast UHPLC method for quantifying six common parabens (preservatives) in cosmetic products. The UHPLC method using a 1.9 μm column achieved a run time of 3.5 minutes, an over 80% reduction compared to the 19 minute run time using a conventional HPLC column. The UHPLC method also reduced solvent usage by over 90%. The method was shown to be linear, precise, and able to detect parabens at levels within EU and FDA regulations using samples of face lotion and body lotion.
New Software Methods Enhance Sedimentation Velocity Analysis of Protein Aggre...KBI Biopharma
1) New software methods have improved the resolution and sensitivity of sedimentation velocity analysis, making it useful for comparability testing, formulation development, and quality control.
2) A method developed by Peter Schuck increases resolution of multiple components and detects very minor components below 1%. It provides higher resolution than SEC columns.
3) Another new method allows obtaining conformation and mass information at extremely low concentrations below 1 microgram total protein.
1) A study was conducted to reverse engineer an oral suspension containing a poorly water soluble drug to match the dissolution profile of the reference listed drug (RLD) using quality by design principles.
2) Two multivariate design of experiments (DoEs) were used, along with live in-situ dissolution monitoring using fiber optic probes, to identify formulation factors influencing dissolution and reduce development time.
3) The approach resulted in a new formulation matching the RLD dissolution profile within 72 hours, much faster than traditional methods requiring costly and time-consuming HPLC analysis.
Filtration Strategies for Optimal Development and Purification of a FMD Virus...MilliporeSigma
This poster presentation outlines the different filtration strategies and performances in the upstream and downstream process to develop a scalable, cost-efficient and GMP-compliant Foot and Mouth Disease (FMD) vaccine production:
• Introduction to foot and mouth disease (FMD) and background on FMD vaccines
• Review of cell culture, clarification, and concentration/diafiltration steps for the production and purification of the FMD vaccine
• Suggested further actions based on data outlined in this poster
To learn more about this topic or collaborate with our technical experts, schedule an in-person or remote visit at our M Lab™ Collaboration Centers: www.emdmillipore.com/mlab
Similar to Compatibility of DMSO spotting buffers with Nexterion Slide E Epoxy-coated Microarray slides (15)
Effect of Detergent Concentration and Type when printing on Epoxy Coated NEXT...SCHOTT
This document summarizes the results of an experiment testing the effect of detergent concentration and type on signal intensity and spot morphology when printing oligonucleotides on NEXTERION® Slide E microarray slides. The experiment found that Triton X-100 at concentrations between 0.015-0.04% produced uniform spot intensity, shape, and diameter with low variability in spot sizes. Both detergent concentration and type influenced spot size and signal intensity, with lower concentrations producing less uniform spots and higher concentrations risking irregular spot morphology. The optimal detergent must be determined empirically based on the specific coating, probes, and printing method used.
Customer Newsletter September 2007
Contents
• Short Profile Director of R&D and Tech support
• Nitrocellulose Slides
• Customized solutions
• Application report: Nexterion 70mer Kit
• News and Exhibition calendar
Customrer Newsletter September 2007
Contents
• Short Profile Director of R&D and Tech support
• Nitrocellulose Slides
• Customized solutions
• Application report: NEXTERION 70mer Kit
• News and Exhibition calendar
Newsletter September 2006
Contents
• SCHOTT Profile Sales and Tech Support North America
• Comarative Genomic Hybridization
• New low evaporation spotting buffer
• News and Exhibition calendar
Perkin Elmer HydroGel slide and the SCHOTT alternative (Slide H)SCHOTT
The document compares the Perkin Elmer HydroGel microarray slide to the SCHOTT Nexterion Slide H as an alternative. Both slides can be used for protein microarrays and small molecules, though the SCHOTT slide has a thinner, less fragile coating and requires fewer pre-treatment steps. The SCHOTT slide protocols differ from the Perkin Elmer protocols and should be followed for the SCHOTT slide.
Epoxy silane (NEXTERION Slide E) versus Amino-silane Microarray Slides: Which...SCHOTT
Many researchers still use aminosilane coated slides for microarray production by immobilizing the DNA probes on the surface via ionic interactions. However, epoxysilane coated slides, which allow to link probes
covalently, becoming more and more accepted.
The aim of this study was to compare both slides in their performance by using oligonucleotides (probe and target) as test system.
We wanted to discover if there is a difference between both surfaces in terms of
I) Binding capacity and signal intensity,
II) Binding specificity,
III) Background performance
IV) Reproducibility.
We used NEXTERION Slide E (epoxy) and NEXTERION Slide A+ (aminosilane) which are produced using identical production processes and analogous silanes. Therefore, these two slides types represent a suitable model to investigate the questions asked. In addition, epoxy and amine slides from other suppliers were included into the test. We discuss how these differences affect the degree of discrimination and specificity of microarray based experiments.
SCHOTT Glass Substrates for Microfluidic ApplicationsSCHOTT
SCHOTT produces glass substrates well-suited for microfluidic systems. They offer various glass types and formats including flat glass sheets, wafers, and a photostructurable glass ceramic. SCHOTT uses several melting technologies for their flat glass including microfloat, down-draw, up-draw, and float processes. Their glass substrates offer properties like chemical inertness and optical transparency that make them suitable for microfluidics.
SCHOTT NEXTERION® HiSens optical coating for high sensitive microarray analysisSCHOTT
The reflective layers on Nexterion® HiSens significantly enhance sensitivity and signal response. The characteristics of the reflective layers have been optimized for the fluorescent wavelengths most commonly used in microarray experiments, and will simultaneously improve the performance in both the Cy3™ and Cy5™ channels. The slide is produced according to industry standard slide dimensions and is available with SCHOTT’s standard high quality functional coatings for DNA and protein microarraying. This means that the Nexterion® HiSens Slide is fully compatible with all microarray printing technologies and most slide processing protocols, allowing customers a smooth transition from industry-standard transparent microarray slides.
NEXTERION® Slide P - The slide of choice for Antibody MicroarraysSCHOTT
The document describes the Nexterion Slide P, a slide coating for antibody microarrays. It has a proprietary polymer coating that provides very low non-specific binding. Probes can be attached to the NHS-ester functional groups on the coating. The slide demonstrates excellent spot morphology and binding capacity up to 1mg/ml probe concentration. It also shows extremely low background signals and superior signal-to-background ratios compared to other slides, making it well-suited for antibody microarrays.
Protein microarrays allow the immobilization and detection of large numbers of proteins on small surfaces. Three key steps in the protein microarray workflow are printing, surface selection, and imaging. Optimizing the printing process is important to minimize contamination between samples. The Omnigrid and Microgrid systems can print contact-style onto 3D substrates with controls to reduce surface damage. Multiplexed protein microarrays on plates allow high-throughput screening by testing many samples in parallel. NovaRay imaging supports multiple array formats and wavelengths for detection. An example experiment showed specific and reproducible detection of target proteins in individual wells of a multiplexed plate with no carryover between wells.
Modification to the Nexterion Spot Spotting buffer compositionSCHOTT
Nexterion Spot is a twofold concentrated spotting solution that can be modified to create different solutions by adding detergents. Nexterion Spot I is identical to the base Nexterion Spot solution, while Nexterion Spot II and III are prepared by adding cetyltrimethylammoniumbromide or Triton X-100 respectively to Nexterion Spot in concentrations of 0.02%. Nexterion Spot AM I contains 6xSSC with 3 M betaine instead of Nexterion Spot, and Nexterion Spot AM II is prepared by adding betaine to Nexterion Spot. The detergent concentration can be varied between 0.01-0.1% for modifying spot size.
This document provides a bibliography of 10 publications that cite the use of SCHOTT Nexterion products. The publications cover a range of topics including:
1. Methods for distinguishing between types of lung cancer.
2. Analysis conditions for profiling mammalian tissue and cell extracts using antibody microarrays.
3. Isolating proteins from mammalian tissue for analysis on antibody microarrays.
4. Genes controlling metabolism in Corynebacterium glutamicum.
The bibliography lists the authors, year, title, journal, digital object identifier, and whether there is open/free access to the publication. The majority of citations do not provide open access to the full publication.
Schott Nexterion provides glass substrates for microarrays and cleans them using an automated ultrasonic cleaning process in a class 100 clean room. The cleaning process uses multiple wash steps with solutions at varying pH levels, rinse steps, and final drying. Schott Nexterion offers high flexibility and a comprehensive product line of coated slides to meet various microarray applications.
Overview of SCHOTT’s protein micro-array slide surfacesSCHOTT
Choosing the best slide coating chemistry for your protein micro-array application. Poster presented at the Advances in Microarray Technology 2008 conference in Barcelona, Spain.
SCHOTT Nexterion Slide H poster presented at PepTalk 2004SCHOTT
SCHOTT Nexterion Slide H is a slide surface ideally suited for the printing of protein microarrays, as it is ideally suited for the covalent immobilization of peptides and proteins such as antibodies, antibody fragments, enzymes, or receptors. For many protein microarray applications Nexterion® Slide H has proven to be a very attractive alternative to the commonly used nitrocellulose coated slide, especially where low background, or slide transparency are important considerations. Since its introduction, the slide coating has also been successfully used with amino-modified oligonucleotides, and has become the slide of choice for printing amino-linked glycan microarrays.
Carbohydrate arrays are a rapidly growing area of microarray research and Nexterion® Slide H is an excellent choice for use in the rapid screening of carbohydrateprotein interactions. The permeable, polymer coating has a large immobilization capacity, and helps to preserve the native three-dimensional structure of complex bio-molecules, thus maintaining conformation and functionality.
Nexterion® Slide H produces excellent signal-to-background ratios and an exceptionally wide dynamic range compared to conventional “two-dimensional” coatings through a unique combination of low non-specific binding characteristics, and high probe loading capacity. Even very low intensity signals, such as those obtained from low-abundance analytes, or weakly expressed genes can be reliably detected and quantified on Nexterion® Slide H. The robust coating matrix is fully compatible with commercial microarray printers and scanners. Simple and robust protocols are available making Nexterion® Slide H easy-to-use.
"Choosing proper type of scaling", Olena SyrotaFwdays
Imagine an IoT processing system that is already quite mature and production-ready and for which client coverage is growing and scaling and performance aspects are life and death questions. The system has Redis, MongoDB, and stream processing based on ksqldb. In this talk, firstly, we will analyze scaling approaches and then select the proper ones for our system.
zkStudyClub - LatticeFold: A Lattice-based Folding Scheme and its Application...Alex Pruden
Folding is a recent technique for building efficient recursive SNARKs. Several elegant folding protocols have been proposed, such as Nova, Supernova, Hypernova, Protostar, and others. However, all of them rely on an additively homomorphic commitment scheme based on discrete log, and are therefore not post-quantum secure. In this work we present LatticeFold, the first lattice-based folding protocol based on the Module SIS problem. This folding protocol naturally leads to an efficient recursive lattice-based SNARK and an efficient PCD scheme. LatticeFold supports folding low-degree relations, such as R1CS, as well as high-degree relations, such as CCS. The key challenge is to construct a secure folding protocol that works with the Ajtai commitment scheme. The difficulty, is ensuring that extracted witnesses are low norm through many rounds of folding. We present a novel technique using the sumcheck protocol to ensure that extracted witnesses are always low norm no matter how many rounds of folding are used. Our evaluation of the final proof system suggests that it is as performant as Hypernova, while providing post-quantum security.
Paper Link: https://eprint.iacr.org/2024/257
Taking AI to the Next Level in Manufacturing.pdfssuserfac0301
Read Taking AI to the Next Level in Manufacturing to gain insights on AI adoption in the manufacturing industry, such as:
1. How quickly AI is being implemented in manufacturing.
2. Which barriers stand in the way of AI adoption.
3. How data quality and governance form the backbone of AI.
4. Organizational processes and structures that may inhibit effective AI adoption.
6. Ideas and approaches to help build your organization's AI strategy.
FREE A4 Cyber Security Awareness Posters-Social Engineering part 3Data Hops
Free A4 downloadable and printable Cyber Security, Social Engineering Safety and security Training Posters . Promote security awareness in the home or workplace. Lock them Out From training providers datahops.com
Introduction of Cybersecurity with OSS at Code Europe 2024Hiroshi SHIBATA
I develop the Ruby programming language, RubyGems, and Bundler, which are package managers for Ruby. Today, I will introduce how to enhance the security of your application using open-source software (OSS) examples from Ruby and RubyGems.
The first topic is CVE (Common Vulnerabilities and Exposures). I have published CVEs many times. But what exactly is a CVE? I'll provide a basic understanding of CVEs and explain how to detect and handle vulnerabilities in OSS.
Next, let's discuss package managers. Package managers play a critical role in the OSS ecosystem. I'll explain how to manage library dependencies in your application.
I'll share insights into how the Ruby and RubyGems core team works to keep our ecosystem safe. By the end of this talk, you'll have a better understanding of how to safeguard your code.
How information systems are built or acquired puts information, which is what they should be about, in a secondary place. Our language adapted accordingly, and we no longer talk about information systems but applications. Applications evolved in a way to break data into diverse fragments, tightly coupled with applications and expensive to integrate. The result is technical debt, which is re-paid by taking even bigger "loans", resulting in an ever-increasing technical debt. Software engineering and procurement practices work in sync with market forces to maintain this trend. This talk demonstrates how natural this situation is. The question is: can something be done to reverse the trend?
Building Production Ready Search Pipelines with Spark and MilvusZilliz
Spark is the widely used ETL tool for processing, indexing and ingesting data to serving stack for search. Milvus is the production-ready open-source vector database. In this talk we will show how to use Spark to process unstructured data to extract vector representations, and push the vectors to Milvus vector database for search serving.
Digital Marketing Trends in 2024 | Guide for Staying AheadWask
https://www.wask.co/ebooks/digital-marketing-trends-in-2024
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Compatibility of DMSO spotting buffers with Nexterion Slide E Epoxy-coated Microarray slides
1. November 2004DMSO Spotting BufferMicroarray Solution
SCHOTT Jenaer Glas GmbH
Final Report
DMSO Spotting Buffers on Slide E
Norman Rahnis, Kornelia Kuhn, Katrin Steinmetzer
Application Support/R&D
Microarray Solutions
Schott Jenaer Glas GmbH
2. November 2004DMSO Spotting BufferMicroarray Solution
SCHOTT Jenaer Glas GmbH
Spotting Solution
Components DMSO, SSC, phosphate buffer, SDS
Slides Nexterion Slide E
Probes 70mer (from E. coli Genome Oligo Set Version 1.0) 10 µM
Spotting QArray Mini, 22°C, 45% RH, SMP3 pin (Telechem), aQu Split
Pin K2801 (diameter 150 µm)
Target total RNA (E.coli) was used to synthesize aa-cDNA, the coupling
of NHS-ester-dyes (Nexterion Dye3 and Dye5) was performed
with a standard protocol derived from a Cy3/Cy5 labeling
protocol,
Hybridization Tecan Hybridization Station HS4800, in 5xSSC, 0.1% SDS, 0.1
mg/ml herring sperm DNA, 50% formamide, 42°C, 16 hrs
Scanning Affymetrix Array Scanner 428
Analysis IconoClust
Experimental Details
3. November 2004DMSO Spotting BufferMicroarray Solution
SCHOTT Jenaer Glas GmbH
....some remarks
the following images show results after hybridization
blue spots mean lower signal, green spots mean higher signals
suitable buffers were highlighted in red
spot diameter was only determined for good spots
4. November 2004DMSO Spotting BufferMicroarray Solution
SCHOTT Jenaer Glas GmbH
Results SMP3 Pin 50% DMSO
50% DMSO 3x SSC
50% DMSO 1.5x SSC, spot diameter 70 µm
50% DMSO 150 mM PP, 0.1% SDS
50% DMSO 150 mM PP, 0.05% SDS
50% DMSO 150 mM PP
50% DMSO
5. November 2004DMSO Spotting BufferMicroarray Solution
SCHOTT Jenaer Glas GmbH
Results SMP3 Pin 30% DMSO
30% DMSO 3x SSC
30% DMSO 1.5x SSC,
spot diameter 70 µm
30% DMSO 150 mM PP, 0.1% SDS
30% DMSO 150 mM PP, 0.05% SDS
30% DMSO 150 mM PP
30% DMSO
6. November 2004DMSO Spotting BufferMicroarray Solution
SCHOTT Jenaer Glas GmbH
Results SMP3 Pin
25% DMSO 0.005% SDS
50% DMSO 1.5x SSC, 0.005% SDS,
spot diameter 60-70 µm
25% DMSO 1.5xSSC, 0.005% SDS
Spot diameter 90 µm
50% DMSO 75 mM PP
best results were obtained when combining DMSO with SSC
and with or without SDS (but only for f.c. SDS of 0.005%, higher
concentrations lead to precipitation)
a combination of DMSO, phosphate buffer and SDS does not
work
further tests with additional combinations of DMSO, SSC and
other detergents will be tested
7. November 2004DMSO Spotting BufferMicroarray Solution
SCHOTT Jenaer Glas GmbH
Results SMP3 Pin
50% DMSO 2x SSC
best results were obtained when combining DMSO with SSC
and with or without detergents
spot sizes range between 100 and 140 µm
similar results were obtained with 30% DMSO
50% DMSO 1.5x SSC, 0.025% CTAB
50% DMSO 1.5x SSC, 0.025% Triton X100
50% DMSO 2.5x SSC
50% DMSO 1.5x SSC, 0.005% SDS
8. November 2004DMSO Spotting BufferMicroarray Solution
SCHOTT Jenaer Glas GmbH
Results aQu K2801 Pin 50% DMSO
50% DMSO 3x SSC
50% DMSO 1.5x SSC
50% DMSO 150 mM PP, 0.1% SDS
50% DMSO 150 mM PP, 0.05% SDS
50% DMSO 150 mM PP
50% DMSO
no suitable buffer combinations
9. November 2004DMSO Spotting BufferMicroarray Solution
SCHOTT Jenaer Glas GmbH
Results aQu K2801 Pin 30% DMSO
30% DMSO 3x SSC
spot diameter 360 µm
30% DMSO 1.5x SSC,
spot diameter 90 µm
30% DMSO 150 mM PP, 0.1% SDS
30% DMSO 150 mM PP, 0.05% SDS
30% DMSO 150 mM PP
30% DMSO
30% DMSO with 1.5x SSC gives good results, further SSC-
concentrations will be tested
10. November 2004DMSO Spotting BufferMicroarray Solution
SCHOTT Jenaer Glas GmbH
Results aQu K2801 Pin
25% DMSO 0.005% SDS
50% DMSO 1.5x SSC, 0.005% SDS,
spot diameter 110 µm
25% DMSO 1.5xSSC, 0.005% SDS
Spot diameter 160 µm
50% DMSO 75 mM PP
best results were obtained when combining DMSO with SSC
and with or without SDS (but only for f.c. SDS of 0.005%, higher
concentrations lead to precipitation)
a combination of DMSO, phosphate buffer and SDS does not
work
further tests with additional combinations of DMSO, SSC and
other detergents will be tested
11. November 2004DMSO Spotting BufferMicroarray Solution
SCHOTT Jenaer Glas GmbH
Results aQu K2801 Pin
50% DMSO 1.5x SSC, 0.025% CTAB
50% DMSO 1.5x SSC, 0.025% Triton X100
50% DMSO 2.5x SSC
50% DMSO 2x SSC
best results were obtained when combining DMSO with SSC
and with or without detergents
spot sizes range between 100 and 140 µm
similar results were obtained with 30% DMSO
50% DMSO 1.5x SSC, 0.005% SDS
12. November 2004DMSO Spotting BufferMicroarray Solution
SCHOTT Jenaer Glas GmbH
Summary
50 (30%) DMSO in water usually gives very small spots on Slide E
(approximately 60 µm)
addition of SSC (f.c. 1.5x to 2.5x) with or without detergent (f.c. 0.005% to
0.025%) gives good spot morphology and size
spot size can be increased by increasing SSC concentration and/or adding
detergent
50(30)% DMSO is not compatible with Next Spot or phosphate buffer (150
mM f.c. phosphate buffer), addition of detergent does not improve spot
morphology*
Editor's Notes
The complete labeling protocol as pdf is available upon request
Lot-Numbers of dyes used:
Dye 3E08-03158
Dye 5E06-04115
Cy3311839
Cy5311840
Alexa 55502E2-1
Alexa 64702E1-1
spotting buffers from second test
all combinations of DMSO, SSC and 0.05% or 0.1% SDS precipitated and could not be tested
spotting buffers from second test
all combinations of DMSO, SSC and 0.05% or 0.1% SDS precipitated and could not be tested
selected spotting buffers from first test
selected spotting buffers from first test
CTAB = cetyl- trimethylammonium bromide
spotting buffers from second test
all combinations of DMSO, SSC and 0.05% or 0.1% SDS precipitated and could not be tested
spotting buffers from second test
all combinations of DMSO, SSC and 0.05% or 0.1% SDS precipitated and could not be tested
selected spotting buffers from first test
selected spotting buffers from first test
CTAB = cetyl- trimethylammonium bromide