The document compares the Perkin Elmer HydroGel microarray slide to the SCHOTT Nexterion Slide H as an alternative. Both slides can be used for protein microarrays and small molecules, though the SCHOTT slide has a thinner, less fragile coating and requires fewer pre-treatment steps. The SCHOTT slide protocols differ from the Perkin Elmer protocols and should be followed for the SCHOTT slide.

1. For immediate release
Perkin Elmer HydroGel slide and the SCHOTT alternative
Introduction
SCHOTT can offer an alternative to the PE slide: SCHOTT Nexterion Slide H.
PE microarray slide PE Product Equivalent SCHOTT SCHOTT
Code slide Product Code
HydroGel coated slides- 6050017 Nexterion Slide H bar- 1070936
Single pad (25 per box) no coded 25 slides per
barcode pack
Further Nexterion Slide H information & protocols are available on
http://www.schott.com/nexterion/english/
http://www.schott.com/nexterion/english/download/protokol_slide_h.pdf
Comparison PE HydroGel Slide and SCHOTT Slide H performances
Perkin Elmer HydroGel Nexterion Slide H Comments
coated slides
Applications Protein microarrays Protein microarrays Both PE HydroGel
Small molecules and Slide H may
(peptides, sugars, etc.) be used for
arraying proteins
without the need
to modify the
probes.
Coating type 3-Dimensional surface 3-Dimensional surface The PE surface is
and The HydroGel gel is 4 Slide H coating is 10 - very fragile when
thickness microns thick when dry 60 nm. hydrated meaning
and 20-30µm thick when that it can be very
hydrated. easily damaged
by contact
printers. The
Slide H surface is
much thinner, and
less prone to
damage.
However due the
reduced
thickness,
customers will
need to re
calibrate the
printing (Z axis)
height when they
change to Slide H

2. For immediate release
Pre printing The PE HydroGel slides Slide H may be used
treatment have a protective storage immediately from the
step agent applied to the packaging (after
surface after bringing the package
manufacture. This layer up to room
has to be removed in a temperature. Slide H
four-step procedure. . must be stored at -20'C
). No pre-treatment is
required
Chemical The PE HydroGel slide Slide H is based on a Typically the non-
composition uses an acrylamide- type of polymer specific binding on
based polymer coating specifically chosen for Slide H is much
bound to a glass slide. its extremely low non- lower than on
specific binding. acrylamide-based
polymers.
Binding Proteins are irreversibly Slide H has a high Slide H will
capacity immobilized on the PE binding capacity as a typically show a
slide within the porous result of having NHS- higher signal to
HydroGel substrate, ester active groups for background ratios
through interactions with covalently binding bio that the PE
the matrix. The molecules. After HydroGel.
recommended blocking arraying, the residual
for the PE is a "physical" NHS ester groups can
blocking method using be efficiently
PBST + 1%BSA. Non- chemically deactivated
specific binding of serum by treating with 50 mM
proteins can still occur on ethanolamine in buffer.
the blocked HydroGel
coating, leading to a
higher background.
Protocols For details see below For details see below The PE HydroGel
and Slide H
protocols are very
different, and we
would only
recommend using
the SCHOTT Slide
H protocols with
the SCHOTT
slides.

3. For immediate release
Comparison PE HydroGel Slide and SCHOTT Slide H Protocols
Step PE HydroGel SCHOTT Slide H
Storage (of unprinted +20°C, sealed Short term: (< 2 week) +20°C,
slides) dry and sealed
Long term: sealed, -20°C
Pre-printing wash Required (to remove storage Not required
procedure agent)
• 3 x (briefly) dH2O
• 3 x 10 min dH2O
• 6 x (briefly) dH2O
• spin dry: 1000-1500
RCF, 5 min
• 20 min, 40°C
Print buffer • Phosphate / borate Recommended printing buffer
buffer systems (for contact printing)
• Glycerol up to 40% 150 mM Phosphate, pH 8.5, 5 %
• PH stability 5-9 Glycerol, 0,1 mg/ml BSA, 0,01%
Sarcosyl
Immobilization 8-16 h at 30°C, Over night at 20°C,
approximately 65-70% air approximately 65-70% air
humidity (saturated sodium humidity (saturated sodium
chloride solution) chloride solution)
• 20 sek PBST (0.5%)
• 3 x 30 min PBST
Blocking / Optional Required (to deactivate
Deactivation • PBST with 1% BSA, remaining NHS ester groups)
1h, RT PBST (0.01%) with 50 mM
• 3 x (briefly) PBST Ethanolamine
PBST, 5 min
dH2O, 1 min
Target Incubation Recommended dilution Recommended dilution buffer
buffer for target / anatlyte: for target / anatlyte:
• PBST • PBST (0.05%)
Washes: Washes:
• (Briefly) PBST (0.5%) • PBST (1%), 2 min
• 3 x 10 min PBST • PBST (0.5%), 2 min
(0.1%) • PBST (0.1%), 2 min
• 2 x (briefly) dH2O
spin dry (e.g. 1000 RPM, 2 min)
Date: 12. Februar 2007