Innoscan microarray Scanner with 785 nm Infra-red option
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SCHOTT NEXTERION® HiSens optical coating for high sensitive microarray analysis
The reflective layers on Nexterion® HiSens significantly enhance sensitivity and signal response. The characteristics of the reflective layers have been optimized for the fluorescent wavelengths most commonly used in microarray experiments, and will simultaneously improve the performance in both the Cy3™ and Cy5™ channels. The slide is produced according to industry standard slide dimensions and is available with SCHOTT’s standard high quality functional coatings for DNA and protein microarraying. This means that the Nexterion® HiSens Slide is fully compatible with all microarray printing technologies and most slide processing protocols, allowing customers a smooth transition from industry-standard transparent microarray slides.
Effect of Detergent Concentration and Type when printing on Epoxy Coated NEXT...
This document summarizes the results of an experiment testing the effect of detergent concentration and type on signal intensity and spot morphology when printing oligonucleotides on NEXTERION® Slide E microarray slides. The experiment found that Triton X-100 at concentrations between 0.015-0.04% produced uniform spot intensity, shape, and diameter with low variability in spot sizes. Both detergent concentration and type influenced spot size and signal intensity, with lower concentrations producing less uniform spots and higher concentrations risking irregular spot morphology. The optimal detergent must be determined empirically based on the specific coating, probes, and printing method used.
Microarray Solutions newsletter #5
Newsletter May 2009
Contents
• OEM coated coverslips
• Diagnostic microarray based applications
• News and Exhibition calendar
Microarray Solutions newsletter #3
Customer Newsletter September 2007
Contents
• Short Profile Director of R&D and Tech support
• Nitrocellulose Slides
• Customized solutions
• Application report: Nexterion 70mer Kit
• News and Exhibition calendar
Microarray Solutions newsletter #2
Customrer Newsletter September 2007
Contents
• Short Profile Director of R&D and Tech support
• Nitrocellulose Slides
• Customized solutions
• Application report: NEXTERION 70mer Kit
• News and Exhibition calendar
Microarray Solutions newsletter #1
Newsletter September 2006
Contents
• SCHOTT Profile Sales and Tech Support North America
• Comarative Genomic Hybridization
• New low evaporation spotting buffer
• News and Exhibition calendar
Perkin Elmer HydroGel slide and the SCHOTT alternative (Slide H)
The document compares the Perkin Elmer HydroGel microarray slide to the SCHOTT Nexterion Slide H as an alternative. Both slides can be used for protein microarrays and small molecules, though the SCHOTT slide has a thinner, less fragile coating and requires fewer pre-treatment steps. The SCHOTT slide protocols differ from the Perkin Elmer protocols and should be followed for the SCHOTT slide.
Epoxy silane (NEXTERION Slide E) versus Amino-silane Microarray Slides: Which...
Many researchers still use aminosilane coated slides for microarray production by immobilizing the DNA probes on the surface via ionic interactions. However, epoxysilane coated slides, which allow to link probes
covalently, becoming more and more accepted.
The aim of this study was to compare both slides in their performance by using oligonucleotides (probe and target) as test system.
We wanted to discover if there is a difference between both surfaces in terms of
I) Binding capacity and signal intensity,
II) Binding specificity,
III) Background performance
IV) Reproducibility.
We used NEXTERION Slide E (epoxy) and NEXTERION Slide A+ (aminosilane) which are produced using identical production processes and analogous silanes. Therefore, these two slides types represent a suitable model to investigate the questions asked. In addition, epoxy and amine slides from other suppliers were included into the test. We discuss how these differences affect the degree of discrimination and specificity of microarray based experiments.
Recommended
SCHOTT NEXTERION® HiSens optical coating for high sensitive microarray analysis
The reflective layers on Nexterion® HiSens significantly enhance sensitivity and signal response. The characteristics of the reflective layers have been optimized for the fluorescent wavelengths most commonly used in microarray experiments, and will simultaneously improve the performance in both the Cy3™ and Cy5™ channels. The slide is produced according to industry standard slide dimensions and is available with SCHOTT’s standard high quality functional coatings for DNA and protein microarraying. This means that the Nexterion® HiSens Slide is fully compatible with all microarray printing technologies and most slide processing protocols, allowing customers a smooth transition from industry-standard transparent microarray slides.
Effect of Detergent Concentration and Type when printing on Epoxy Coated NEXT...
This document summarizes the results of an experiment testing the effect of detergent concentration and type on signal intensity and spot morphology when printing oligonucleotides on NEXTERION® Slide E microarray slides. The experiment found that Triton X-100 at concentrations between 0.015-0.04% produced uniform spot intensity, shape, and diameter with low variability in spot sizes. Both detergent concentration and type influenced spot size and signal intensity, with lower concentrations producing less uniform spots and higher concentrations risking irregular spot morphology. The optimal detergent must be determined empirically based on the specific coating, probes, and printing method used.
Microarray Solutions newsletter #5
Newsletter May 2009
Contents
• OEM coated coverslips
• Diagnostic microarray based applications
• News and Exhibition calendar
Microarray Solutions newsletter #3
Customer Newsletter September 2007
Contents
• Short Profile Director of R&D and Tech support
• Nitrocellulose Slides
• Customized solutions
• Application report: Nexterion 70mer Kit
• News and Exhibition calendar
Microarray Solutions newsletter #2
Customrer Newsletter September 2007
Contents
• Short Profile Director of R&D and Tech support
• Nitrocellulose Slides
• Customized solutions
• Application report: NEXTERION 70mer Kit
• News and Exhibition calendar
Microarray Solutions newsletter #1
Newsletter September 2006
Contents
• SCHOTT Profile Sales and Tech Support North America
• Comarative Genomic Hybridization
• New low evaporation spotting buffer
• News and Exhibition calendar
Perkin Elmer HydroGel slide and the SCHOTT alternative (Slide H)
The document compares the Perkin Elmer HydroGel microarray slide to the SCHOTT Nexterion Slide H as an alternative. Both slides can be used for protein microarrays and small molecules, though the SCHOTT slide has a thinner, less fragile coating and requires fewer pre-treatment steps. The SCHOTT slide protocols differ from the Perkin Elmer protocols and should be followed for the SCHOTT slide.
Epoxy silane (NEXTERION Slide E) versus Amino-silane Microarray Slides: Which...
Many researchers still use aminosilane coated slides for microarray production by immobilizing the DNA probes on the surface via ionic interactions. However, epoxysilane coated slides, which allow to link probes
covalently, becoming more and more accepted.
The aim of this study was to compare both slides in their performance by using oligonucleotides (probe and target) as test system.
We wanted to discover if there is a difference between both surfaces in terms of
I) Binding capacity and signal intensity,
II) Binding specificity,
III) Background performance
IV) Reproducibility.
We used NEXTERION Slide E (epoxy) and NEXTERION Slide A+ (aminosilane) which are produced using identical production processes and analogous silanes. Therefore, these two slides types represent a suitable model to investigate the questions asked. In addition, epoxy and amine slides from other suppliers were included into the test. We discuss how these differences affect the degree of discrimination and specificity of microarray based experiments.
SCHOTT Glass Substrates for Microfluidic Applications
SCHOTT produces glass substrates well-suited for microfluidic systems. They offer various glass types and formats including flat glass sheets, wafers, and a photostructurable glass ceramic. SCHOTT uses several melting technologies for their flat glass including microfloat, down-draw, up-draw, and float processes. Their glass substrates offer properties like chemical inertness and optical transparency that make them suitable for microfluidics.
NEXTERION® Slide P - The slide of choice for Antibody Microarrays
The document describes the Nexterion Slide P, a slide coating for antibody microarrays. It has a proprietary polymer coating that provides very low non-specific binding. Probes can be attached to the NHS-ester functional groups on the coating. The slide demonstrates excellent spot morphology and binding capacity up to 1mg/ml probe concentration. It also shows extremely low background signals and superior signal-to-background ratios compared to other slides, making it well-suited for antibody microarrays.
Protein Microarrays: Approaches to Printing
Protein microarrays allow the immobilization and detection of large numbers of proteins on small surfaces. Three key steps in the protein microarray workflow are printing, surface selection, and imaging. Optimizing the printing process is important to minimize contamination between samples. The Omnigrid and Microgrid systems can print contact-style onto 3D substrates with controls to reduce surface damage. Multiplexed protein microarrays on plates allow high-throughput screening by testing many samples in parallel. NovaRay imaging supports multiple array formats and wavelengths for detection. An example experiment showed specific and reproducible detection of target proteins in individual wells of a multiplexed plate with no carryover between wells.
NEXTERION® Microarray slides with barcodes and custom logos from SCHOTT
Details about the barcodes and custom logos SCHOTT can apply to borosilicate glass microarray slides.
Compatibility of DMSO spotting buffers with Nexterion Slide E Epoxy-coated Mi...
Compatibility of DMSO spotting buffers with SCHOTT Nexterion Epoxy-silane coated microarray slides. Effect of using different spotting pins and DMSO concentrations.
Modification to the Nexterion Spot Spotting buffer composition
Nexterion Spot is a twofold concentrated spotting solution that can be modified to create different solutions by adding detergents. Nexterion Spot I is identical to the base Nexterion Spot solution, while Nexterion Spot II and III are prepared by adding cetyltrimethylammoniumbromide or Triton X-100 respectively to Nexterion Spot in concentrations of 0.02%. Nexterion Spot AM I contains 6xSSC with 3 M betaine instead of Nexterion Spot, and Nexterion Spot AM II is prepared by adding betaine to Nexterion Spot. The detergent concentration can be varied between 0.01-0.1% for modifying spot size.
Protocol for Preparation of Streptavidin-coated Slides based on SCHOTT Nexter...
Protocol for preparation of streptavidin-coated slides at point of use by Prof Jonathan Blackburn; University of Cape Town
Publications citing schott_nexterion_microarray_products_20100929
This document provides a bibliography of 10 publications that cite the use of SCHOTT Nexterion products. The publications cover a range of topics including:
1. Methods for distinguishing between types of lung cancer.
2. Analysis conditions for profiling mammalian tissue and cell extracts using antibody microarrays.
3. Isolating proteins from mammalian tissue for analysis on antibody microarrays.
4. Genes controlling metabolism in Corynebacterium glutamicum.
The bibliography lists the authors, year, title, journal, digital object identifier, and whether there is open/free access to the publication. The majority of citations do not provide open access to the full publication.
Nexterion glass cleaning_2009
Schott Nexterion provides glass substrates for microarrays and cleans them using an automated ultrasonic cleaning process in a class 100 clean room. The cleaning process uses multiple wash steps with solutions at varying pH levels, rinse steps, and final drying. Schott Nexterion offers high flexibility and a comprehensive product line of coated slides to meet various microarray applications.
Overview of SCHOTT’s protein micro-array slide surfaces
Choosing the best slide coating chemistry for your protein micro-array application. Poster presented at the Advances in Microarray Technology 2008 conference in Barcelona, Spain.
SCHOTT Nexterion Slide H poster presented at PepTalk 2004
SCHOTT Nexterion Slide H is a slide surface ideally suited for the printing of protein microarrays, as it is ideally suited for the covalent immobilization of peptides and proteins such as antibodies, antibody fragments, enzymes, or receptors. For many protein microarray applications Nexterion® Slide H has proven to be a very attractive alternative to the commonly used nitrocellulose coated slide, especially where low background, or slide transparency are important considerations. Since its introduction, the slide coating has also been successfully used with amino-modified oligonucleotides, and has become the slide of choice for printing amino-linked glycan microarrays.
Carbohydrate arrays are a rapidly growing area of microarray research and Nexterion® Slide H is an excellent choice for use in the rapid screening of carbohydrateprotein interactions. The permeable, polymer coating has a large immobilization capacity, and helps to preserve the native three-dimensional structure of complex bio-molecules, thus maintaining conformation and functionality.
Nexterion® Slide H produces excellent signal-to-background ratios and an exceptionally wide dynamic range compared to conventional “two-dimensional” coatings through a unique combination of low non-specific binding characteristics, and high probe loading capacity. Even very low intensity signals, such as those obtained from low-abundance analytes, or weakly expressed genes can be reliably detected and quantified on Nexterion® Slide H. The robust coating matrix is fully compatible with commercial microarray printers and scanners. Simple and robust protocols are available making Nexterion® Slide H easy-to-use.
SCHOTT Nexterion Micro-array Glass Substrates Presentation
SCHOTT established a dedicated microarray solutions unit in 2002. In 2004, they opened the world's most advanced microarray slide production plant in Jena, Germany. SCHOTT leverages their glass manufacturing and coating expertise to produce a broad range of high-quality microarray substrates. Their substrates and technical support services help customers with DNA and protein microarray applications.
SCHOTT Nexterion® Glass B surface flatness
SCHOTT BOROFLOAT 33 glass is manufactured using the microfloat method, which produces flat glass sheets with a mirror-like surface quality. The flatness of glass sheets can be influenced by various parameters such as warp, bow, total thickness variation, thickness tolerance, and thickness deviation. For Nexterion Glass B slides, flatness is defined as the accumulated thickness deviation including warp, intra-slide thickness deviation, and inter-slide thickness tolerance, which is typically less than or equal to 25 μm combined. Measurement using interferometry shows that the typical flatness across a single 75.6 x 25 mm Nexterion Glass B slide is less than or equal to 5 μm. The surface microroughness of Glass B
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SCHOTT Glass Substrates for Microfluidic Applications
SCHOTT produces glass substrates well-suited for microfluidic systems. They offer various glass types and formats including flat glass sheets, wafers, and a photostructurable glass ceramic. SCHOTT uses several melting technologies for their flat glass including microfloat, down-draw, up-draw, and float processes. Their glass substrates offer properties like chemical inertness and optical transparency that make them suitable for microfluidics.
NEXTERION® Slide P - The slide of choice for Antibody Microarrays
The document describes the Nexterion Slide P, a slide coating for antibody microarrays. It has a proprietary polymer coating that provides very low non-specific binding. Probes can be attached to the NHS-ester functional groups on the coating. The slide demonstrates excellent spot morphology and binding capacity up to 1mg/ml probe concentration. It also shows extremely low background signals and superior signal-to-background ratios compared to other slides, making it well-suited for antibody microarrays.
Protein Microarrays: Approaches to Printing
Protein microarrays allow the immobilization and detection of large numbers of proteins on small surfaces. Three key steps in the protein microarray workflow are printing, surface selection, and imaging. Optimizing the printing process is important to minimize contamination between samples. The Omnigrid and Microgrid systems can print contact-style onto 3D substrates with controls to reduce surface damage. Multiplexed protein microarrays on plates allow high-throughput screening by testing many samples in parallel. NovaRay imaging supports multiple array formats and wavelengths for detection. An example experiment showed specific and reproducible detection of target proteins in individual wells of a multiplexed plate with no carryover between wells.
NEXTERION® Microarray slides with barcodes and custom logos from SCHOTT
Details about the barcodes and custom logos SCHOTT can apply to borosilicate glass microarray slides.
Compatibility of DMSO spotting buffers with Nexterion Slide E Epoxy-coated Mi...
Compatibility of DMSO spotting buffers with SCHOTT Nexterion Epoxy-silane coated microarray slides. Effect of using different spotting pins and DMSO concentrations.
Modification to the Nexterion Spot Spotting buffer composition
Nexterion Spot is a twofold concentrated spotting solution that can be modified to create different solutions by adding detergents. Nexterion Spot I is identical to the base Nexterion Spot solution, while Nexterion Spot II and III are prepared by adding cetyltrimethylammoniumbromide or Triton X-100 respectively to Nexterion Spot in concentrations of 0.02%. Nexterion Spot AM I contains 6xSSC with 3 M betaine instead of Nexterion Spot, and Nexterion Spot AM II is prepared by adding betaine to Nexterion Spot. The detergent concentration can be varied between 0.01-0.1% for modifying spot size.
Protocol for Preparation of Streptavidin-coated Slides based on SCHOTT Nexter...
Protocol for preparation of streptavidin-coated slides at point of use by Prof Jonathan Blackburn; University of Cape Town
Publications citing schott_nexterion_microarray_products_20100929
This document provides a bibliography of 10 publications that cite the use of SCHOTT Nexterion products. The publications cover a range of topics including:
1. Methods for distinguishing between types of lung cancer.
2. Analysis conditions for profiling mammalian tissue and cell extracts using antibody microarrays.
3. Isolating proteins from mammalian tissue for analysis on antibody microarrays.
4. Genes controlling metabolism in Corynebacterium glutamicum.
The bibliography lists the authors, year, title, journal, digital object identifier, and whether there is open/free access to the publication. The majority of citations do not provide open access to the full publication.
Nexterion glass cleaning_2009
Schott Nexterion provides glass substrates for microarrays and cleans them using an automated ultrasonic cleaning process in a class 100 clean room. The cleaning process uses multiple wash steps with solutions at varying pH levels, rinse steps, and final drying. Schott Nexterion offers high flexibility and a comprehensive product line of coated slides to meet various microarray applications.
Overview of SCHOTT’s protein micro-array slide surfaces
Choosing the best slide coating chemistry for your protein micro-array application. Poster presented at the Advances in Microarray Technology 2008 conference in Barcelona, Spain.
SCHOTT Nexterion Slide H poster presented at PepTalk 2004
SCHOTT Nexterion Slide H is a slide surface ideally suited for the printing of protein microarrays, as it is ideally suited for the covalent immobilization of peptides and proteins such as antibodies, antibody fragments, enzymes, or receptors. For many protein microarray applications Nexterion® Slide H has proven to be a very attractive alternative to the commonly used nitrocellulose coated slide, especially where low background, or slide transparency are important considerations. Since its introduction, the slide coating has also been successfully used with amino-modified oligonucleotides, and has become the slide of choice for printing amino-linked glycan microarrays.
Carbohydrate arrays are a rapidly growing area of microarray research and Nexterion® Slide H is an excellent choice for use in the rapid screening of carbohydrateprotein interactions. The permeable, polymer coating has a large immobilization capacity, and helps to preserve the native three-dimensional structure of complex bio-molecules, thus maintaining conformation and functionality.
Nexterion® Slide H produces excellent signal-to-background ratios and an exceptionally wide dynamic range compared to conventional “two-dimensional” coatings through a unique combination of low non-specific binding characteristics, and high probe loading capacity. Even very low intensity signals, such as those obtained from low-abundance analytes, or weakly expressed genes can be reliably detected and quantified on Nexterion® Slide H. The robust coating matrix is fully compatible with commercial microarray printers and scanners. Simple and robust protocols are available making Nexterion® Slide H easy-to-use.
SCHOTT Nexterion Micro-array Glass Substrates Presentation
SCHOTT established a dedicated microarray solutions unit in 2002. In 2004, they opened the world's most advanced microarray slide production plant in Jena, Germany. SCHOTT leverages their glass manufacturing and coating expertise to produce a broad range of high-quality microarray substrates. Their substrates and technical support services help customers with DNA and protein microarray applications.
SCHOTT Nexterion® Glass B surface flatness
SCHOTT BOROFLOAT 33 glass is manufactured using the microfloat method, which produces flat glass sheets with a mirror-like surface quality. The flatness of glass sheets can be influenced by various parameters such as warp, bow, total thickness variation, thickness tolerance, and thickness deviation. For Nexterion Glass B slides, flatness is defined as the accumulated thickness deviation including warp, intra-slide thickness deviation, and inter-slide thickness tolerance, which is typically less than or equal to 25 μm combined. Measurement using interferometry shows that the typical flatness across a single 75.6 x 25 mm Nexterion Glass B slide is less than or equal to 5 μm. The surface microroughness of Glass B
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How to Get CNIC Information System with Paksim Ga.pptx
Pakdata Cf is a groundbreaking system designed to streamline and facilitate access to CNIC information. This innovative platform leverages advanced technology to provide users with efficient and secure access to their CNIC details.
みなさんこんにちはこれ何文字まで入るの？40文字以下不可とか本当に意味わからないけどこれ限界文字数書いてないからマジでやばい文字数いけるんじゃないの？えこ...
ここ3000字までしか入らないけどタイトルの方がたくさん文字入ると思います。
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Cosa hanno in comune un mattoncino Lego e la backdoor XZ?
ABSTRACT: A prima vista, un mattoncino Lego e la backdoor XZ potrebbero avere in comune il fatto di essere entrambi blocchi di costruzione, o dipendenze di progetti creativi e software. La realtà è che un mattoncino Lego e il caso della backdoor XZ hanno molto di più di tutto ciò in comune.
Partecipate alla presentazione per immergervi in una storia di interoperabilità, standard e formati aperti, per poi discutere del ruolo importante che i contributori hanno in una comunità open source sostenibile.
BIO: Sostenitrice del software libero e dei formati standard e aperti. È stata un membro attivo dei progetti Fedora e openSUSE e ha co-fondato l'Associazione LibreItalia dove è stata coinvolta in diversi eventi, migrazioni e formazione relativi a LibreOffice. In precedenza ha lavorato a migrazioni e corsi di formazione su LibreOffice per diverse amministrazioni pubbliche e privati. Da gennaio 2020 lavora in SUSE come Software Release Engineer per Uyuni e SUSE Manager e quando non segue la sua passione per i computer e per Geeko coltiva la sua curiosità per l'astronomia (da cui deriva il suo nickname deneb_alpha).
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みなさんこんにちはこれ何文字まで入るの？40文字以下不可とか本当に意味わからないけどこれ限界文字数書いてないからマジでやばい文字数いけるんじゃないの？えこ...
みなさんこんにちはこれ何文字まで入るの？40文字以下不可とか本当に意味わからないけどこれ限界文字数書いてないからマジでやばい文字数いけるんじゃないの？えこ...
Cosa hanno in comune un mattoncino Lego e la backdoor XZ?
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