Column chromatography is a chromatography method used to isolate individual chemical compounds from mixtures. It involves packing a glass column with silica gel as the stationary phase, then adding the compound mixture to the top of the column. As the mobile phase solvent moves through the column, the different compounds separate into bands based on their varying degrees of adhesion to the silica gel. The individual bands can then be collected and the desired product isolated from the mixture.

1. COLUMN CHROMATOGRAPHY
Presented By-
Dip Roy
BSc Student
Department of Chemistry
University of Barishal,Bangladesh

2. Column chromatography was devoloped by the American chemist D.T Day in
1900 .M.S. Tswett, the Polish botanist, in 1906 used adsorption columns in
his investigations of plant pigments.

3. COLUMN CHROMATOGRAPHY
Column chromatography is a chromatography method used to
isolate a single chemical compound from a mixture. In order to
actually perform the separation, a glass column is assembled
(as shown in the picture). A glass column was taken with a
stopcock attached at the bottom, inserted a cotton plug at the
bottom of the column and packed the column with a slurry of
silica gel (prepared in an organic solvent).

4. COLUMN CHROMATOGRAPHY
Once the column was packed, poured the
reaction mixture over the bed of silica from the
top of the column carefully, with the aid of a glass
pipette. Then the stopcock was opened and let
the solvent run slowly through the column and
constantly kept adding solvent from the top of the
glass column.

5. COLUMN CHROMATOGRAPHY
The reaction mixture started separating into three
distinct bands respectively. The individual bands was
collected in separate flasks and was thus able to
obtain desired product. The different components of
the analyte exhibit varying degrees of adhesion to the
silica, and as a result they travel at different speeds
through the stationary phase as the solvent flows
through it, indicated by the separation of the different
bands.

6. COLUMN CHROMATOGRAPHY
Higher the adsorption to the
stationary phase, the slower the
molecule will move through the
column. Higher the solubility in the
mobile phase, the faster the molecule
will move through the column.

7. THANK YOU