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Growing Cells in Lab Conditions
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2. A 3D cell culture grown with magnetic levitation (Image: G. Souza/N3D Biosciences)
3. GROWING CONDITIONS NATURAL MEDIA :FLUIDS OBTAINED FROM LIVING SYSTEMS FOR EG LYMPH ,BLOOD , SERUM AND EMBRYO HOMOGENATES NUTRIENT MEDIA :IT IS THE MOST COMMON MEDIA WHICH CONSIST OF CARBON, SALT WATER AS WELL AS AMINOACID NITROGEN MINIMAL MEDIA : MINIMUM NUTRIENTS NEEDED FOR GROWTH WITHOUT THE PRESCENCE OF AMINO ACIDS SELECTIVE MEDIA :IT IS UDED TO CULTURE SELECTIVE ORGANISM COMMONLY USED BY MICROBIOLOGIST
4. TYPES OF CELL CULTURE MASS CULTURE PRIMARY CULTURE CLONAL CULTURE MASS CULTURE :Large numbers of cells are added to a culture dish where they attach to the bottom. Those cells that survive will proliferate and after a number of generations form a monolayer of cells that cover the bottom of dish CLONAL CULTURE : Cell is attached to a dish at some distance from its neighbors. Surviving cells proliferate and form a colony (a clone) whose members are derived from same cell SECONDARY CULTURE : Previously culture cells are removed and transferred to a media is secondary culture
![CELL CULTURE GROWTH OF MAINTAINING DIFFRENT KINDS OF CELLS IN AN ARTIFICIAL ENVIRONMENT UNDER LABORATORCONDITIONS ,[object Object], COMMON METHOD GLASS OR PLASTIC SURFACE IMMERSED IN NUTRIENT FLUID ALLOWS CELLS TO PROLIFERATE](https://image.slidesharecdn.com/cellculture-110831014857-phpapp02/85/Cell-culture-1-320.jpg)
