This clinical audit examines the prevention of cross contamination of Clostridium difficile at Citizens Hospital from 2022-2023. The audit aims to ensure early isolation of C. difficile positive patients, educate staff on prevention precautions, and adhere to infection control guidelines. Among the 12 C. difficile positive patients audited, all required cross contamination precautions were promptly followed by staff, including proper isolation, use of PPE, hand hygiene, and cleaning/disinfection of equipment. The results showed no instances of cross contamination from these patients. Teamwork and staff education on prevention strategies were emphasized.