1) CHO cells are commonly used for recombinant protein production due to their ability to produce human-like proteins through gene amplification methods and their resistance to human pathogens. 2) General strategies for CHO cell line development include transfection of the gene of interest, selection of high-producing clones through gene amplification markers like DHFR, and single cell cloning to isolate high-producing cell lines. 3) Transfected CHO cells are cultured in medium containing selection agents like methotrexate to stimulate amplification of the gene of interest along with the DHFR gene. Clones with the highest protein titers are selected for scale-up in bioreactors.