Performance report of a Biodegradable Industrial degreaser

1. RMC manufactured -DfE Biozyme - Biodegradable Industrial degreaser
A compilation by Hussam Adeni
hussam_adeni@yahoo.com Lubricant Specialist & Tribologist
Trial and Performance report
RMC product
DfE Biozyme
Biodegradable
Industrial Heavy Duty Degreaser.
Hussam Adeni – Compilation
(Collated during my tenure with Reza Industrial Solution.
Jeddah Saudi Arabia, 2014-15)

2. www.rezahygiene.com
Jeddah
Tel: +966 12 657 2877
Fax: +966 12 657 2832
hygiene@rezagroup.com
U.A.E.
Tel: +971 4 380 5715
Fax: +971 4 380 5905
hygieneuae@rezagroup.com
Riyadh
Tel: +966 11 479 1906
Fax: +966 11 478 9853
hygiene@rezagroup.com
Qatar
Tel: +974 4 44160824
Fax: + 974 4 44160513
hygieneqatar@rezagroup.com
Al-Khobar
Tel: +966 13 802 4860
Fax: +966 13 802 1302
hygiene@rezagroup.com
Bahrain
Tel: +973 17 699125
Fax: +973 17 699061
hygienebh@rezagroup.com

3. September 23, 2013
DfE Biozyme M Specifications
• Flammability: Non-flammable
• Maximum Storage Temperature: 120 degrees Fahrenheit
• Minimum Storage Temperature: 35 degrees Fahrenheit
• Optimal Storage Temperature: 40-100 degrees Fahrenheit
• Shelf Life: 2 years when stored as recommended
• Toxicity
Material Tested Species LC50 (ppm)
DfE Biozyme M Menidia beryllina 38.24 96-hr
DfE Biozyme M Mysidopsis bahia 38.94 48-hr
No. 2 Fuel Oil Menidia beryllina 3.76 96-hr
No. 2 Fuel Oil Mysidopsis bahia 2.04 48-hr
DfE Biozyme M & No 2 Fuel Oil (1:10) Menidia beryllina 6.88 96-hr
DfE Biozyme M & No 2 Fuel Oil (1:10) Mysidopsis bahia 2.14 48-hr
Reference Toxicant Menidia beryllina 12.19 96-hr
(Sodium Laurel Sulfate) Mysidopsis bahia 10.53 48-hr
Note: Toxicity data was derived using the concentrated product. Aquatic toxicity
test method: US EPA 40 CFR Chapter 1, Part 300 Appendix C, Item 3.0: Revised
Standard Dispersant Toxicity Test.
• Physical/Chemical Properties
Flash Point: Not Determined
Pour Point: None
Viscosity: Like Water
Specific Gravity: Approximately 1
pH: 8.5-9.5
www.rochestermidland.com
Corporate Headquarters
155 Paragon Drive
Rochester, NY 14624 USA
Tel: 585.336.2200
Fax: 585.467.4406

4. Surface Active Agents: Proprietary
Solvents; Proprietary
Additives: Proprietary
Solubility: Complete in Water
This formulation is a trade secret and comprised of a mixture of anionic and
nonionic surfactants, preservatives, micronutrients and bacteria (Bacillus
pumilus, Brevibacillus parabrevis, Paenibacillus validus) in water. The strains in
this product have not been genetically modified or engineered. The species used
in the product are recognized as Class 1 microorganisms (Risk Group 1;
Biosafety Group 1) which means they pose little or no threat to humans.
• Biodegradability
This product contains Anionic Alcohol Ethoxylates and Dodecyl Benzene Sulfonic
Acids; both classes of surfactants are regarded as readily biodegradable using
the OECD Closed Bottle Test.
• Analysis for Heavy Metals, Cyanide, and Chlorinated Hydrocarbons
Compound Concentration (ppm)
Arsenic 0.0098
Cadmium <0.0020
Chromium 0.1190
Copper 0.0180
Lead <0.0040
Mercury <0.010
Nickel <0.0040
Zinc 0.0606
Cyanide <0.020
Chlorinated Hydrocarbons <1.0
Polychlorinated Biphenyls <0.100

5.
REZA Hygiene Technical Report
Field demonstrations of Biozyme M
Report Date 18/5/13
Report No. RH2
Author Andrew Turner
Issued to Prasad Dias
Keith Watson
Investigation
We performed 3 separate tests on the Biozyme M product across 2 days. These
test involved the following;
- Test 1 – Demonstration of product on fresh oil and crud removed from an
offshore tank. This was poured over a steel pipe and left to dry for an
hour before demonstrating the products ability.
- Test 2 – The product was tested on 2 onshore well heads which had
recently had oil leaks
- Test 3 – The product was demonstrated on an offshore platform on 2
wellheads. These had sunbaked crude oil, dust and salt crust covering
the equipment
All tests were carried out using the same batch of Biozyme M and dosed using a
simple pump up sprayer. In a simple test of the products ability no proper
agitation was used on any of the tests with the exception of high pressure hosing
on the final offshore test site.

6.
Results
Test 1 – Product Demonstration
On this test fresh crude and silt from an offshore tank was poured over a
stainless pipe and left to dry on for approximately 1 hour.
Test details
Product Concentration 10% (1:10) made up with sweet water
Dosing method Portable pump up sprayer
Contact time 1-2 minutes
Agitation None used. Product just wiped off
Rinsing None used
The product was sprayed onto the area required to be cleaned and had
immediate results as the crude started to come away from the steel tube straight
away. After a dwell time of 1-2 minutes the tube was wiped cleaned with a rag
leaving no trace of the crude oil at all.
Fig. 1 Applying the product

7.
Test 2 – Field Trial on onshore Wellheads
Following the successful demonstration of the Biozyme M in a controlled
environment we ventured out in the field to a wellhead that needed cleaning after
suffering minor leaks.
Test details
Product Concentration 10% (1:10) made up with sweet water
Dosing method Portable pump up sprayer
Contact time 5-10 minutes
Agitation None used. Product just wiped off
Rinsing None used
The wellhead was coated in oil that had started to dry out but retained some of its
tackiness. The entire wellhead was sprayed with the Biozyme M solution and
left to dwell for 5-10 minutes. We then wiped clean all the easily reachable areas
with rack with a few repeat sprayings on thick areas of crude.
The result was instant with the crude simply wiping away leaving the painted
wellhead surface completely clean in most areas. Some areas would need more
agitation and some sort of pressure rinsing but over all a good standard of clean
was achieved in no more than 15 minutes. The top 60cm of the wellhead was
not accessible.
Fig 2. Before cleaning Fig 3. After cleaning
Fig 2. Welhead prior to treatment

8.
Test 3 – Field Trial on offshore wellheads
Following the successful trials onshore it was decided that we should trial the
Biozyme M product offshore on a wellhead platform. A suitable platform was
chosen that had not been cleaned in a longtime. The platform in question had up
to 2-3cm of baked on crude oil on the wellheads that was also covered in a thick
layer of dust and salt.
Test details
Product Concentration 10% made up with salt water
50% & Neat also used
Dosing method Portable pump up sprayer
Contact time 30 minutes
Agitation None used
Rinsing High pressure jetting
Initially we treated a wellhead with the same 10% solution used onshore left for
10-15 minutes minutes then rinsed off using high pressure jetting. The result
was not as immediate as when used on the fresher crude but it did clean faster
than normal jetting and leave a better result.
As the trial progressed due to the difficulty in getting fresh water to mix the
product we used it at higher concentrations and even applied neat in areas. It
should be noted that Biozyme M will not increase in benefit as the concentration
goes beyond 40-50%. The best range for use is 10-40% so a good supply of
water is needed to make up solutions.
We performed various test including cleaning half of a wellhead with just high
pressure hosing and half treated with Biozyme M. The result was noticeably
improved using Biozyme M on a number of counts;
- Time to remove the crude was much shorter
- Overall appearance of the wellhead was better and removed more of the
crude to the painted surface level
- Less work was needed with the high pressure hose. When just using the
hose it was held at 5-10cm from the wellhead to achieve the same result
as 20-30cm away with Biozyme M.

9.
Fig 4. Before Fig 5. After

10.
Fig 6. Before and after when just using 15% Biozyme M

11.
Summary
From our results we can conclude that the Biozyme M works excellently on fresh
spillage cleanup on equipment and older (7-10 day) crude at levels as low as
10% (1:10). These results were achieved using minimal or zero agitation and no
rinsing.
On older baked on crude and higher concentrations of Biozyme M the result was
not as instant but still gave a noticeable improvement in clean, decrease in
cleaning time and decrease in cleaning effort.
With the correct procedure Biozyme M could be used in many different areas to
speed up the cleaning process.
We would recommend a procedure along the lines of:-
1. Pre-Treat the effected area with 10-20% Biozyme M solution. The
solution can be applied via pump up sprayer or buckets and brushes.
2. Leave solution to soak for 20-30 minutes
3. Scrub thicker areas of crude using brushes dipped in buckets of Biozyme
M solution.
4. Rinse using high pressure hosing
5. Repeat if necessary in areas of heavy soiling
Biozyme M has a residual effect so the product will continue to work for a few
days afterwards.
We would recommend using Biozyme M in place of any high hazard chemicals
or traditional detergents used to remove crude oil as it has numerous advantages
over other products, including;
- 100% Biodegradable. Waste biodegrades to just CO2 and H2O
- pH 7.5 – 8.5
- Minimal (0) to slight (1) NFPA HMIS hazard codes
- VOC compliant
- EcoLogo certified
- Degrades Polyaromatic hydrocarbons, Phenol, Cresol and BETEX
compounds
- Can be used offshore with no need to retain rinsewater
If the end goal is to remove organic solvents, caustics, acids and petrochemical
based cleaners then a bacteria based biologic cleaning product is the only step
to take.

12. MATERIAL SAFETY DATA SHEET
204/13/2012
DATE PRINTED: 04/23/2012
REVISION DATE:
PREPARED BY: EH&S DEPARTMENT
REVISION NUMBER:
1. CHEMICAL PRODUCT
PRODUCT NAME:
DFE BIOZYME M, Bio-Remediator
PRODUCT CODE: 119276
NFPA/HMIS HAZARD CODES(minimal=0; slight=1; moderate=2; serious=3; severe=4)
2. HAZARDS IDENTIFICATION
EFFECTS FROM ACUTE EXPOSURE:
INGESTION: Causes vomiting, nausea, and diarrhea.
SKIN CONTACT: May cause mild skin irritation.
INHALATION: May irritate mouth, nose, and throat.
EYE CONTACT: May cause mild eye irritation.
CHRONIC EFFECTS: Dermatitis. Possible respiratory damage from inhalation of dust or mist.
EFFECTS/CARCINOGENICITY: None listed under OSHA, IARC, or NTP.
ROUTES OF ENTRY: Routes of entry for solids and liquids include eye and skin contact, ingestion and inhalation.
3. COMPOSITION/INFORMATION ON INGREDIENTS
PRODUCT COMPOSITION
CAS#
% ACGIH TLV OSHA PELs
Viable Bacteria Cultures
MIXTURE
<1 Not applicable Not applicable
4. FIRST AID MEASURES
INGESTION: Rinse mouth with water many times. Drink several glasses of water or milk.
SKIN: Wash with soap and water.
INHALATION: Move person to fresh air.
EYES: Immediately flush eyes with large amounts of water for at least 15 minutes. Get immediate
medical attention.
NOTES TO PHYSICIAN: None.
5. FIRE AND EXPLOSION HAZARD DATA
METHOD: None
FLAMMABLE LIMITS IN AIR
Page 1 of 3
Reactivity: 0/0 Special/Protective Equipment: None/B
Health:
FLASH POINT:
NAME OF THE
MANUFACTURER:
(F): Not determined (C): Not
determined
Rochester Midland Corporation
155 Paragon Drive
Rochester, New York 14624
USA
1/1
Information: 585-336-2200
Emergency Phone:
INFOTRAC: 1-800-535-5053
OUTSIDE US: 1-352-323-3500
Fire: 0/0

13. DFE BIOZYME M, Bio-Remediator
____________________________________________________________________________________
LOWER (%): No data
UPPER (%): No data
SENSITIVITY TO MECHANICAL IMPACT(Y/N): NO
SENSITIVITY TO STATIC DISCHARGE: Sensitivity to static discharge is not expected.
SUITABLE EXTINGUISHING MEDIA: Water fog, carbon dioxide, foam, dry chemical.
FIRE FIGHTING PROCEDURES: Fire-fighters should wear self-contained breathing apparatus and
full protective clothing when fighting chemical fires.
6. ACCIDENTAL RELEASE MEASURES
SPILL PROCEDURES:
SMALL SPILLS: Reclaim as much as possible. Flush residue with water.
LARGE SPILLS: Reclaim as much as possible. Shovel or sweep up residue and place in suitable containers.
Flush remainder with water.
PERSONAL PRECAUTIONS: Wear adequate personal protective equipment, see Section 8, Exposure Controls/Personal
Protection.
ENVIRONMENTAL PRECAUTIONS: Not applicable
METHODS FOR CLEANING UP: Not applicable
7. HANDLING AND STORAGE
PRECAUTIONS TO BE TAKEN
IN HANDLING AND STORAGE:
Avoid generating or breathing dust. Store in a well ventilated area. Store in a cool, dry location
away from incompatible materials. Keep container closed when not in use. Store between 32°
F and 120°F. Avoid direct sunlight.
OTHER PRECAUTIONS: No other spill procedures necessary.
SPECIFIC USE(S): Not applicable.
8. EXPOSURE CONTROLS/PERSONAL PROTECTION
PROTECTIVE EQUIPMENT:
EXPOSURE CONTROLS: Use in a well ventilated area.
RESPIRATORY PROTECTION: Use approved NIOSH respiratory protection if TLV/PEL exceeded or if over exposure is likely.
PROTECTIVE GLOVES: Chemical resistant gloves are recommended to minimize skin contact.
EYE PROTECTION: Safety glasses are recommended to minimize eye contact.
OTHER PERSONAL PROTECTION
EQUIPMENT:
Appropriate protective clothing as needed to prevent skin contact. Eyewash fountains and
safety showers must be easily accessible. It is the responsibility of the end user of this product
to determine level of PPE required that is consistent with safe use of this product.
VENTILATION: General mechanical and/or local exhaust as needed to meet exposure limits if mist in air.
9. PHYSICAL AND CHEMICAL PROPERTIES
APPEARANCE AND ODOR: Opaque. Liquid. Bacterial odor.
VAPOR PRESSURE: Not determined
VAPOR DENSITY (AIR=1): Not determined
SOLUBILITY IN WATER: Not determined
SPECIFIC GRAVITY: Not determined
VOC Content (%): Not determined
EVAPORATION RATE: Not determined
PH: 8.5-9.5
10. STABILITY AND REACTIVITY
STABILITY DATA: STABLE
Page 2 of 3
BOILING POINT (F): (C) Not determinedNot determined

14. DFE BIOZYME M, Bio-Remediator
____________________________________________________________________________________
10. STABILITY AND REACTIVITY
POLYMERIZATION: Will Not Occur.
HAZARDOUS DECOMPOSITION: None known.
INCOMPATIBILITY (MATERIALS TO
AVOID):
Contact with oxidizing agents.
CONDITIONS/HAZARDS TO AVOID: Strong acids or alkali compounds may inactivate biological cultures.
11. TOXICOLOGICAL INFORMATION
ACUTE TOXICITY: Not Established
EFFECTS OF CHRONIC EXPOSURE: Not established.
OTHER TOXIC EFFECTS: Not established.
12. ECOLOGICAL INFORMATION
ECOTOXICOLOGICAL
INFORMATION:
No data at this time
CHEMICAL FATE INFORMATION: No data at this time.
MOBILITY: Not applicable.
PERSISTENCE/DEGRADABILITY: Not applicable
BIOACCUMULATIVE POTENTIAL: Not applicable
OTHER ADVERSE EFFECTS: Not applicable
13. DISPOSAL CONSIDERATIONS
WASTE DISPOSAL METHODS: Dispose in accordance with Federal, State and Local regulations.
14. TRANSPORT INFORMATION
Please refer to the Bill of Lading/Receiving documents for up to date shipping information.
15. REGULATORY INFORMATION
PRODUCT COMPOSITION
CAS#
% TSCA: EINECS: Canada DSL: CA PROP 65:
Viable Bacteria Cultures
MIXTURE
<1 Not Listed Not Listed Not Listed Not Listed
PRODUCT COMPOSITION
CAS#
% CERCLA: SARA 302: SARA 313:
Viable Bacteria Cultures
MIXTURE
<1 Not Listed Not Listed Not Listed
None of the components of this material are included in the Massachusetts Substance List nor are present at or above reportable
levels.
None of the components of this material are included in the New Jersey Substance List nor are present at or above reportable levels.
None of the components of this material are included in the Pennsylvania Substance List nor are present at or above reportable levels.
16. OTHER INFORMATION
This information was compiled from current, reliable sources and is believed to be correct. As data, and/or regulations change, and
conditions of use and handling are beyond our control, no warranty, express or implied, is made as to completeness or continuing
accuracy of this information.
*** END OF MSDS ***
Page 3 of 3

15. Networking – Memberships and Certificates Professional:
Hussam Adeni hussam57 Blog URL: http://lubricantspecialist.wordpress.com/
www.stle.org
www.lubecouncil.com
Society of Tribologists & Lubrication Engineer: Member – Individual
Membership number - 49912 M Certification: MLA 1
Section - International (99). Certified Level 1 Machinery Lubricant
Analyst, equivalent to Category I of
ISO 18436-4
For details of my prowess on the net, please feel free to browse the web links.
http://www.slideshare.net/hussam57/testing-of-lubes-and-its-significance-nov-2011
http://www.slideshare.net/hussam57/basic-of-lubricants-amp-lubrication
http://lubricantspecialist.wordpress.com/2011/10/25/6/ http://twitter.com/#!/hussamadeni
Invite you to Google search for "Hussam Adeni" for blogs & posts of myweb activity.