This document provides instructions for setting up and using Virtuoso Opensource Triplestore (VOS) and related tools. It describes how to install VOS, configure it using a PHP manager script to run multiple instances, load Bio2RDF data into the instances using a loader script, and access the data via SPARQL endpoints. Step-by-step instructions are given for installing VOS, loading DrugBank data as an example, and a live demo is referenced.