A closed-system for HPLC can provide several benefits over an open-system by protecting mobile phases from contamination which can damage columns. A closed-system prevents issues like particulates from entering the flow and causing thousands of dollars in costs and troubleshooting time. It also avoids safety hazards from open bottles and maintains consistency by keeping the mobile phase intact. The benefits of a closed-system include consistency, increased instrument and lab up time, lack of bacteria and fungus, extended column life, returning 25% of analysts' time to the lab, and reduced operator training.