Basic Tests for Pharmaceutical substances Basic Tests for Pharmaceutical substances Basic Tests for Pharmaceutical substances Basic Tests for Pharmaceutical substances

1. BASIC TESTS FOR DRUGS-
TEST PROCEDURES FOR
PHARMACEUTICAL SUBSTANCES
Mr. Shubham Sharma
Assistant Professor
SOP, Mangalayatan University, Aligarh

2. These tests are performed for the identification of pharmaceutical
substances, plant materials and dosage forms.

3. AMIKACIN SULFATE
Description- A white to yellowish- white, crystalline powder, almost odorless.
Melting Point – About 204°C IdentityTest 1 -
Dissolve 10mg Amikacin sulfate in 1ml of water
Add 1ml of Sodium Hydroxide solution and mix
Then add 2ml cobalt nitrate solution
A violet colour is produced, which shows the presence of Amikacin sulfate

4. IdentityTest- 2
Dissolve 0.05gm of Amikacin sulfate in 3ml of water
Add slowly 4ml of anthrone
A bluish violet colour is produced, which confirms the presence of Amikacin sulfate

5. Dissolve 20mg Amikacin sulfate in 1ml of water
Add 1ml of Barium chloride solution
A white ppt is produced, confirms the presence of Amikacin sulfate
IdentityTest 3 –

6. CAPTOPRIL
Description- A white or almost white, crystalline powder, odor – characteristic
Melting Point- About 108°C
IdentityTest 1 -
Dissolve 10mg captopril in 2ml of hydrochloric acid solution
Add about 1ml of iodine
The colour of the iodine disappears immediately and a white, turbid solution is produced,
which confirms the presence of captopril

7. IdentityTest 2 –
Dissolve 10mg captopril in 2ml of water
And add 0.5ml of lead acetate solution
A white ppt id produced, shows the presence of captopril

8. CISPLATIN
Description- White to yellowish crystals or a yellow powder
Melting Point- About 270°C
IdentityTests 1 -
Dissolve 5mg of cisplatin in 5ml of hydrochloric acid solution
And heat to boiling to half of the solution (Keep the unused portion
for test 2)
Add a few crystal of potassium iodide
A brownish yellow colour is produced which changes to reddish-
brown on standing, which confirms the presence cisplatin

9. IdentityTest 2 –
To the remaining solution, from test 1 add few crystals of tin chloride
A reddish- orange colour is produced which changes to reddish- brown on
standing, which shows the presence of cisplatin

10. KETAMINE HYDROCHLORIDE
Description- A white, hygroscopic, crystalline powder, characteristic odor
Melting Point- About 260°C
IdentityTest 1 –
Dissolve 10mg ketamine hydrochloride in 4ml of water
Add 0.5ml of nitric acid solution
Add 0.5 ml of silver nitrate solution,A white curdy ppt is produced.
Separate the ppt, wash it with water and add an excess of ammonia, the ppt
dissolve

11. IdentityTest 2 –
Dissolve 10mg of ketamine hydrochloride in 4ml of sulfuric acid solution
Add 1 drop of potassium iodobismuthate
A reddish-brown ppt is produced

12. MAGNESIUM SULFATE
Description- Colorless crystals or a white, crystalline powder, odorless.
IdentityTest 1 –
Dissolve 10mg Magnesium sulfate in 2ml of water
Add 1ml of ammonia, a white ppt is produced
Add 1ml of ammonium chloride, the ppt is dissolved
Add 1ml of disodium hydrogen phosphate solution
A white ppt is produced

13. IdentityTest 2 –
Dissolve 10mg Magnesium sulfate in 2ml of water and add 3 drops of
titan yellow
And add 2ml of sodium hydroxide solution
A pink color is produced

14. COAL TAR
Shake 1 drop of coal tar with 5ml of ethanol and filter
The filtrate is yellow with a bluish green fluorescence
Which shows the presence of coal tar
Description- Brown- black or black, viscous liquid, odor characteristic and strong.
IdentityTest –

15. THANK YOU