Aflatoxin detection at field level is crucial to reduce the contamination in value chain.Antibody reacts with aflatoxin antigen giving coloured reaction. This is simple to perform, cost effective (<2$),><5 min) and accurate.

1. Locations
ICRISAT - East and Southern
Africa
Regional Hub – Nairobi,
Kenya
Country Offices
Ethiopia – Addis Ababa
Malawi – Lilongwe
Zimbabwe – Bulawayo
Mozambique – Maputo

2. ICRISAT
RESEARCH
OUTPUTS
ICRISAT
RESEARCH
OUTPUTS
WCAWCA
ASIAASIAESAESA
Inter-Regional Collaboration

3. Association studies in
Finger Millet (Striga) and
Sorghum (Striga, Disease
resistance)
Association studies in
Finger Millet (Striga) and
Sorghum (Striga, Disease
resistance)
Whole Genome
sequencing of Finger
Millet
Whole Genome
sequencing of Finger
Millet
QTL mapping of new
sources of resistance to
ELS and GRD in Groundnut
QTL mapping of new
sources of resistance to
ELS and GRD in Groundnut
Association studies in
Chickpea for Agronomic
Traits
Association studies in
Chickpea for Agronomic
Traits
GENOMICS
(Cereals and
Legumes)
GENOMICS
(Cereals and
Legumes)

4. Pigeonpea – Exploring Diversity

5. Pigeonpea – Exploiting Diversity

6. ICPL 86022 Cream Selection from MZ 2/9
Pigeonpea – End-user Traits

7. ICPL 88034 MZ 2/9
Pigeonpea – End-user Traits

8. Disease
resistance
3 new sources of
double
resistance
identified
Disease
resistance
3 new sources of
double
resistance
identified
Heat tolerance
13 high yielding
large-seeded
MABC lines in
Kenya
Heat tolerance
13 high yielding
large-seeded
MABC lines in
Kenya
Scaling out
3 new varieties
released in
Ethiopia
Scaling out
3 new varieties
released in
Ethiopia
Seed Prod.
8t Breeder, 72t
Foundation,
2040t
Certified/QDS
seed
Seed Prod.
8t Breeder, 72t
Foundation,
2040t
Certified/QDS
seed
CHICKPEA

9. LFD- User friendly diagnostic device
Aflatoxin detection at
field level is crucial to
reduce the
contamination in
value chain.
Antibody reacts with aflatoxin antigen giving
coloured reaction. This is simple to perform, cost
effective (<2$), less time consuming (<5 min) and
accurate

10. ICRISAT
LFD
ICRISAT
LFD
Results on Validation of LFD
Lateral flow
immunoassay
Number and % of samples tested for AFB1 in ELISA
Positive (above
20 ppb of AFB1)
Negative (below
20 ppb of AFB1)
Total
Positive (above
20 ppb of AFB1)
25 (50.00 %) 0 (0.00 %) 25 (50.00 %)
Negative (below
20 ppb of AFB1)
0 (0.00 %) 25 (50.00 %) 25 (50.00 %)
Total 25 (50.00 %) 25 (50.00 %) 50 (100.00%)
The figure in parentheses were computed based on the visual scoring of lateral
flow device and quantitative data obtained for ELISA after calculation. The
sensitivity of lateral flow device for AFB1 above 20 ppb in samples are 100 %; the
specificity is also 100 %. The positive predictive value is 100 %. The negative
predictive value is 100 %.
Table 1: sensitivity and specificity of lateral flow immunoassay compared to
ELISA for detecting above 20 ppb of aflatoxin in groundnut samples

11. Major Projects
BMGF
• Harnessing Opportunities for
Productivity Enhancement in Sorghum
and Millets in Sub-Saharan Africa (HOPE
II)
• Tropical Legumes III

12. Scaling-out the Revolving Seed
Fund - Malawi

13. Sorghum seed production -
Kenya

14. Bringing it all together
Changing
Lives in the
Semi-Arid
Tropics
Changing
Lives in the
Semi-Arid
Tropics
Understanding
Market pull for
Grain or Livestock
= (Decision for
investment)
Understanding
Market pull for
Grain or Livestock
= (Decision for
investment)
Improved
Technologies
(Crop-Soil-
Livestock)
Improved
Technologies
(Crop-Soil-
Livestock)
Resilience
(Climate
variability, Yield
stability)
Resilience
(Climate
variability, Yield
stability)

15. CRP –
Flagships,
Regional
collaboration
ICRISAT
Investment
plan –
Strategic
Science
Bilateral
Projects –
inter- and
intra-
regional
Partnerships
– Two-way
(Asia-Africa)
– Breeding,
Genomics,
Modelling
OPPORTUNITIES

16. Thank You