Overview of ResearchIn the USDA Infectious Bacterial Diseases UnitWorkshop: An Integrated Approach to Controlling Brucello...
USDA Animal Health Facilities andUSDA Animal Health Facilities andExpertiseExpertiseState-of-the-art:State-of-the-art:•ABS...
abortusabortus) in the United) in the UnitedStatesStates0200040006000800010000120001982 1985 1988 1991 1994 1997 199919912...
Lipopolysaccharide structureLipopolysaccharide structureof virulent and vaccine strainsof virulent and vaccine strainsLipi...
Efficacy of RB51 as aEfficacy of RB51 as aCalfhood Vaccine for CattleCalfhood Vaccine for CattleAge atVaccinationProtectio...
Distribution of Feral Swine in theDistribution of Feral Swine in theUSUSGPS mapping at http://128.192.20.53/infsms/
Brucellosis in the GreaterBrucellosis in the GreaterYellowstone Area (GYA)Yellowstone Area (GYA)
= =Variance in ImmunologicVariance in ImmunologicResponses between RuminantsResponses between Ruminants==
“Lysine decarboxylase”Ab GAAGATGTGA GGAGTAGGGC AGTAGGGC AGT::::: :::::::: ::::::: ::::AAAGAMel GAAGATGTGA GGAGTAGGGC AGT::...
Alleles(numberofrepeatunitsperlocus)12345678910111213So.CaliforniaSo.FloridaCent.FloridaAlabamKansasArkansasElk-WYOklahoma...
Overview of research in the USDA Infectious Bacterial Diseases Unit
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Overview of research in the USDA Infectious Bacterial Diseases Unit

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Presented by Steven Olsen at a workshop on an integrated approach to controlling brucellosis in Africa, Addis Ababa, Ethiopia, 29-31 January 2013.

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Overview of research in the USDA Infectious Bacterial Diseases Unit

  1. 1. Overview of ResearchIn the USDA Infectious Bacterial Diseases UnitWorkshop: An Integrated Approach to Controlling Brucellosisin Africa, Addis Ababa, 29-31 January 2013Dr. Steven OlsenDr. Steven OlsenNational Animal Disease CenterNational Animal Disease CenterAmes, IA 50010Ames, IA 50010
  2. 2. USDA Animal Health Facilities andUSDA Animal Health Facilities andExpertiseExpertiseState-of-the-art:State-of-the-art:•ABSL2 and ABSL3 smallABSL2 and ABSL3 smalland large animal facilitiesand large animal facilities•BSL3 Agriculture largeBSL3 Agriculture largeanimal facilityanimal facilityAnimal care and facilitiesAnimal care and facilitiesoperations expertise thatoperations expertise thatsupports high-containmentsupports high-containmentresearch in all majorresearch in all majorlivestock and severallivestock and severalwildlife specieswildlife species
  3. 3. abortusabortus) in the United) in the UnitedStatesStates0200040006000800010000120001982 1985 1988 1991 1994 1997 199919912011
  4. 4. Lipopolysaccharide structureLipopolysaccharide structureof virulent and vaccine strainsof virulent and vaccine strainsLipid ABrucella abortus strain 19Brucella abortus field strainsBrucella abortus strain RB51Sugars O-side chainKDOLipid A KDO Sugars O-side chainLipid A SugarsKDOThe O-side chain is the immunodominant antigenof Brucella for antibody responses
  5. 5. Efficacy of RB51 as aEfficacy of RB51 as aCalfhood Vaccine for CattleCalfhood Vaccine for CattleAge atVaccinationProtection from abortionRB51 Strain 19 Control% protected (# aborted/ #challenged)10 months 100% (0/20) 100% (0/6) 45% (6/11)7 months 100% (0/22) 100% (0/5) 63% (4/11)5-6 months 92% (2/25) 100% (0/4) 57% (6/14)3 months 87% (2/15) 100% (0/4) 50% (5/10)Overall 95% (4/82) 100% (0/19) 54% (21/46)
  6. 6. Distribution of Feral Swine in theDistribution of Feral Swine in theUSUSGPS mapping at http://128.192.20.53/infsms/
  7. 7. Brucellosis in the GreaterBrucellosis in the GreaterYellowstone Area (GYA)Yellowstone Area (GYA)
  8. 8. = =Variance in ImmunologicVariance in ImmunologicResponses between RuminantsResponses between Ruminants==
  9. 9. “Lysine decarboxylase”Ab GAAGATGTGA GGAGTAGGGC AGTAGGGC AGT::::: :::::::: ::::::: ::::AAAGAMel GAAGATGTGA GGAGTAGGGC AGT::::: :::::::: :::::::: ::::::: ::::AAAGASuis GAAGATGTGA GGAGTAGGGC AGTAGGGC AGTAGGGC AGTAGGGC AGTAGGGC AGTAAAGA
  10. 10. Alleles(numberofrepeatunitsperlocus)12345678910111213So.CaliforniaSo.FloridaCent.FloridaAlabamKansasArkansasElk-WYOklahomaElk-MTBison-MTCent.FloridaCent.Florida14 1717 2121 1616 1919VNTR-1VNTR-1 VNTR-2VNTR-2 VNTR-3VNTR-3 VNTR-4VNTR-4 VNTR-5VNTR-5 VNTR-6VNTR-6 VNTR-7VNTR-7 VNTR-8VNTR-8 VNTR-9VNTR-9 VNTR-10VNTR-10

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