Articulo biología molecular Tercer semestre UPB

1. Mariana Barona Cabrera
Estudiante de medicina
Tercer semestre 2019 - 02

2. Introduction Adenocarcinoma
Is a malignant tumor of a
glandular epithelium.
And now.. What is
PDAC?
Pancreatic ductal
adenocarcinoma?
Ductal pancreatic
cancer cells have
a tendency to
metastasize early
It is a form of
pancreatic cancer
that forms in the
glandular ducts of
the pancreas.
Is a dismal
malignancy with a 5-
year survival rate of
less than 8%

3. Generation of branched
actin networks that power
the protrusion of the
plasma membrane in
lamellipodia.
Introduction
Arpin (Arp2/3 complex
inhibitor), a novel protein
found in 2013, plays a
pivotal role in cell motility
and migration.
What
happen with
arpin?
Arpin
Inhibition Arp 2/3
How is
arpin in
the
tumor?
Arpin
Inhibicion Arp 2/3
Proliferation celular
(Arp2 / 3)
Arpin can negatively
regulate Arp2 / 3
What is
arpin?

4. In this study, was examined the
expression of Arpin in PDAC
tissues and assessed its correlation
with clinicopathological and
prognostic variables in patients
who underwent surgery for PDAC.
Further compared the differences
in Arpin expression between the
primary tumor and metastatic sites
Objective

5. MethodsPatients and sample
collection

6. Methods
Immunohistochemistry
For what?
Which is the
fundament?
Detect, amplify and make visible
a specific antigen, which is
usually a protein.
It is based on the Antigen-
Antibody reaction.

7. Methods
Western
blot
Detect specific proteins in a defined
sample
• Reaction between antigen and antibody
• Protein separation by electrophoresis
• They are transferred to a filter with
antibodies that react with the protein of
interest, in this case is the arpin
For what?
Which is the
fundament?

8. MethodsStatistical
analyses
Fisher exact test.
Chisquare test
Log-rank test
It is a hypothesis test that
compares the observed
distribution of the data with an
expected distribution of the data.
It is a test of statistical significance
used in the analysis of contingency
tables.
It is a statistical technique to
contrast the survival functions of
two populations.

9. Results

10. Results

11. Results

12. Results

13. D
I
S
C
U
S
S
I
O
N
Author Contribution
Dang I
The absence of Arpin can
induce faster
lamellipodia protrusions and
cell migration
Lomakina ME
In breast cancer, loss of Arpin
expression is associated with
enhanced cell growth and larger
tumor size, which supported
our results that low Arpin
expression is related to T stage
of PDAC.
Rotty JD
The Arp2/3 complex is the sole
machinery that generates
branched actin networks. This
machinery is activated at
different
locations of the cell by NPF

14. ConclusionsArpin is diminished in PDAC tissues, which
confirms the rapid metastasis given by PDAC
cells, because when the protein is diminished,
the arp2 / 3 complex will not be inhibited and,
on the contrary, it will be activated in a great
way, generating cell migration.
Molecular biology is important in the clinical
field because it is a science that studies the
processes in the living organism from the
molecular point of view, it also has different
techniques that allows analysis, diagnosis and
treatment of different diseases