2. ANTIFUNGAL ACTIVITY OF BACTERIAL
DERIVER FROM SILVERNANOPARTICLES
AGAINST CAUSING GUVA WILT CAUSING
FUSARIUM SP.
BY
G.ARJUN
II M.Sc. Applied Microbiology
Sacred Heart College (Autonomous)
Tirupattur.
3. INTRODUCTION
Plant disease result in an annual estimate loss of 10 to 15% of the world major crop
with direct economic losses of up to hundred of billion of dollars. Of these disease
70 to 80 % cause by pathogenic fungi. Plant pathogenic fungi have adverse effect
on crop growth and yield. Silver nanoparticles synthesised from bacterial are
applied to agriculture showing good potential result agianst insects, fungi, bacterial,
and viruses. Fusarium species are important plant pathogens causing various diseases
such as crown rot, head blight, and scab on cereal grains (72), and they may
occasionally cause infection in animals (32).
4. AIM
To analyze the antifungal efficacy of biosynthesized silver
nanoparticles against Gauva wilt causing Fusarium sp.
5. OBJECTIVES
To isolate Phytopathogenic fungi from Psidium guajava tree
• To Identify isolated fungi using Morphological and Microscopic observation.
• To perform Preliminary Screening for Silver nanoparticles by collected bacteria.
• To synthesize Silver Nanoparticles Extracellularly from the potent bacterium.
• To characterize the synthesized Silver Nanoparticles.
• To perform antifungal activity of synthesized Silver Nanoparticles against
15. RESULT
The antifungal effect of AgNPs against Fusarium sp. was then performed in
concentration dependant manner. Compared to the control group (no silver
nanoparticles), the growth of Fusarium sp. was obviously inhibited by the
silver nanoparticles in a concentration-dependent manner. The size of the
colonies decreased with the increasing concentration of silver
nanoparticles. When the concentration of silver nanoparticles was 200 μl,
no growth was observed on the plate but when the concentration was 50 μl,
the silver nanoparticles showed no inhibitory effect on colony growth. This
silvernanoparticles in our study showed positive inhibitory effect against
Fusarium sp.