ANAQUANT provides an antibody free protein quantification using mass spectrometry to speed up your R&D projects

1. A trusted CRO to speed up your R&D
programs

2. ANAQUANT ‘At a glance’
! Contract Research Organization
! specialized in targeted and absolute protein
quantification by Mass spectrometry,
! offering customized services in assay development
and samples bioanalysis.
! Supports pharmaceutical and biotech companies to speed
up their R&D programs with robust and validated
analytical tools
! ANAQUANT was founded by Tanguy Fortin in 2014 after
more than ten years of research driven by Pr Jérôme
Lemoine at the «Analytical Sciences Institute » of Lyon.
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3. A purpose for your laboratory
! simplify and accelerate the development of your
bioanalytical tests
! Quickly validate or unvalidate your scientific hypotheses
with limited expenses and risk
! Cross validate your reference method
! Bring additional analytical information like precise
measurement of proteins modifications.
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Whether you are concerned by upfront research, quality control, or clinical study,
we propose you an orthogonal antibody-free alternative to immunoassay
approach in order to:

4. 3 offers to cover your needs
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Evaluation, validation
and quantitation of
protein biomarker(s)
tailored to your clinical
question
Absolute and sensitive
quantification of
targeted protein and
homologs in various
biological matrices
Development of assay
to contribute to Critical
Quality Attribute the
definition of
biotherapeutic and the
bioprocess validation
Biomarker Targeted protein
quantitation
Product Quality &
Bioprocess validation
MULTIPLEXING SPECIFICITY ROBUSTNESS

5. 5
Biomarker
! Single or multiplexed
biomarker analysis
(10 to 100 proteins)
! markerQUANT: a analytical tool
developed with the Camille
Jordan Institute, a worldwide
renowned mathematic institute,
to guarantee accurate and
specific results.
! Discovery of biomarkers thanks
to the fullQUANT approach
Main applications
! disease mechanism study
! i d e n t i f i c a t i o n o f n e w
therapeutic targets
! early assessment of drug
efficacy or safety
! identification of your targets in
body fluids
! development of companion
diagnostic test…

6. ! targetQUANT: precise and
absolute quantification of
targeted protein with or
without post-translational
modifications.
! Based on innovative
technology to ensure specificity
and robustness
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Targeted protein quantification
Main applications
! Level of protein target-ligand
! Early PK/PD assessment
! Measure of post-translational
modification (PTM)
! Cross validation with reference
method
! Transgene expression for gene
therapy drug
! One analysis instead of several
immunoassay kits

7. ! Services based on EMA guidelines
related to biotherapeutics (protein,
Ab, gene therapy, vaccines)
! Goals: to contribute to the Critical
Quality Attribute evaluation of
biotherapeutics & the bioprocess
validation.
! Antibody free analysis to speed up
your bioprocess evaluation
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Product quality & bioprocess validation
Main applications
! Identity test for Ab, proteins,
vaccines
! Specific proteins degradation
pathways
! Potency tests
! Low levels of impurities (HCPs)
! Biosimilar comparability study
! Bioprocess scale-up comparability
study

8. Our know-know at your services
! Regarding your challenge, your concern, ANAQUANT answers with
customized services.
! Milestones with Go/NoGo decision are established for each project to
secure your budget.
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Assay
development
Assay validation
Technology
transfer
Sample
bioanalysis
Feasibility study

9. 9
Main advantages of our technology
No antibody
Multiplexing
Absolute quantification
Short assay development time
Minimal sample volume
Biological complex matrices analysis (serum,
plasma, urine, tissue)

10. The added value of our scientific expertise
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Advices on the protein assay
to implement
Innovative approaches
which improve robustness &
sensitivity of MS analysis
Validated protocols to
control pre-analytical factors
& biological samples
preparation
Method and experience in
data interpretation
Access to:

11. Main publications
! Clinical quantitation of prostate specific antigen biomarker in the low nanogram/mL range by
conventional bore liquid chromatography-tandem mass spectrometry (MRM) coupling and correlation
with ELISA tests
Fortin T, Salvador A, Charrier JP, Lenz C, Lacoux X, Morla A, Choquet-Kastylevsky G, Lemoine J. - Mol Cell Proteomics. 2009
! Multiple reaction monitoring cubed for protein quantification at the low nanogram/milliliter level in
nondepleted human serum.
Fortin T, Salvador A, Charrier JP, Lenz C, Bettsworth F, Lacoux X, Choquet-Kastylevsky G, Lemoine J. - Anal Chem, 2009
! The current status of clinical proteomics and the use of MRM and MRM(3) for biomarker validation
Jérôme Lemoine, Tanguy Fortin, Arnaud Salvador, Aurore Jaffuel, Jean-Philippe Charrier, Geneviève Choquet-Kastylevsky -
Expert Review of Molecular Diagnostics
! Absolute quantification of podocin, a potential biomarker of glomerular injury in human urine, by liquid
chromatography-multiple reaction monitoring cubed mass spectrometry.
Simon R, Lemoine J, Fonbonne C,Jaffuel A, Léonard JF, Gautier JC, Pasquier O, Salvador A. - J Pharm Biomed Anal. 2014

12. To
discuss
about
your
project
please
contact
us:
Tanguy.fortin@anaquant.com
www.anaquant.com