Anthelmintic activity of leaves of different extracts of Gossypium herbaceum ...SriramNagarajan16
The N-hexane, ethyl ether and ethanol extracts of leaves of Gossypium herbaceum L was investigated for
anthelmintic activity using earthworms (Pheretimaposthuma). Various concentrations (10,20,40,60,80 &100
mg/ml) of plant extracts were tested in the bioassay. Albendazole (10 mg/ml) was used as reference standard drug
whereas 1% v/v tween 80 as control. Determination of paralysis time and death time of the worms were recorded.
Theethyl ether and ethanol extracts exhibited significant anthelmintic activity at highest concentration of 60, 80 &
100 mg/ml compared to standard drug. The result shows that ethyl ether extract possesses potent vermicidal
activity and found to be effective as an anthelmintic compared to ethanolic extract
The Central and Peripheral effects of the methanol extract of Fadogia cienkow...iosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Hepato Protective Assessment of Pawpaw Leaves, Neem, Lemon Grass and Acts on ...ijtsrd
Malaria is a major concern in Nigeria, and stands as the second leading cause of death from all infectious disease in Africa. Several studies have reported the damaging effect of the parasite to various body organs especially the liver. Reports over time has shown the benefits of various plants extracts in ethno medicine. However, not much have been done on the effects of some of these extracts in combined form on its hepato protective assessment in comparison with any known ACT based anti malaria. The focus of this study was to explore the hepato protective properties of ethanoic extract of Carica papaya Linn, AzadirachtaIndica, CymbopogonCitratusagainst ACT based antimalarial therapy on plasmodium berghei parasitized wistar rats. Phytochemical analysis of the extracts were done according to the method described by Treaseand Evans. Hepato protective assessment were done using the liver function tests and assay of the liver histology respectively. One hundred and ten 110 rats distributed into 11 groups, each group having 10rats were used for the experiment. Negative control received just feed and water, Positive control were induced with the malaria parasite and given feed and water only. The tests groups were induced with malaria, received feed and water and treated with 500mg kg, 250mg kg and 165mg kg doses of the extracts, both individually and in combined forms, as well as the standard ACT anti malaria. Phytochemical screening showed that the plant extracts possessed high concentration of Tannins, Flavonoids, Saponins and Alkaloids. Plasmodium berghei increased the activities of ALP, ASP and ALT when compared with the positive control group. This may be attributed to increase in functional capacity of the liver as a result of the presence of the infection for the tests groups. Treatment with the plant extracts decreased ALP and ALT levels significantly P 0.05 , as well as AST levels except for the Neem extract. Histological examination of the liver of test animals showed no extensive damage to the tissue by the individual extracts when compared to the negative control group. Nnyaha Anthonia E. | Igbokwe Ugochukwu V. | Okonkwo Onyeka Chukwudi | Ajeka Prisca O. | Nwaissac Ikechukwu S. | Okpa Precious N. "Hepato-Protective Assessment of Pawpaw Leaves, Neem, Lemon Grass and Acts on Plasmodium Berghei Parasitized Wistar Rats" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd46292.pdf Paper URL: https://www.ijtsrd.com/medicine/physiology/46292/hepatoprotective-assessment-of-pawpaw-leaves-neem-lemon-grass-and-acts-on-plasmodium-berghei-parasitized-wistar-rats/nnyaha-anthonia-e
— Post-harvest diseases are a major problem for banana yield. Despite treatments with chemical fungicides, a persistence of diseases is noticed. This study aims at proposing a biological control method against banana post-harvest diseases by using banana rachis leachate. The effect of leachate has been tested in vitro on mycelial growth, conidial germination and in vivo on pathogenic fungi virulence. All leachate concentrations (5, 15 and 20%) tested showed antifungal activity on the tested fungi. However, the 20% concentration was more effective with complete inhibition of mycelial growth and conidial germination of all fungi. No symptoms of crown rot and anthracnose were observed after treatment of bananas with leachate. However, with azoxystrobin, the prevalence of crown rot and anthracnose was 60% and 30%, respectively. Banana rachis leachate recorded highly significant reduction of banana finger rot prevalence compared to azoxystrobin. Banana rachis leachate have strong antifungal properties that may be useful to control banana post-harvest disease as a safe alternative option to chemical fungicides Keywords— banana; post-harvest diseases; banana rachis leachate, antifungal activity.
Anthelmintic activity of leaves of different extracts of Gossypium herbaceum ...SriramNagarajan16
The N-hexane, ethyl ether and ethanol extracts of leaves of Gossypium herbaceum L was investigated for
anthelmintic activity using earthworms (Pheretimaposthuma). Various concentrations (10,20,40,60,80 &100
mg/ml) of plant extracts were tested in the bioassay. Albendazole (10 mg/ml) was used as reference standard drug
whereas 1% v/v tween 80 as control. Determination of paralysis time and death time of the worms were recorded.
Theethyl ether and ethanol extracts exhibited significant anthelmintic activity at highest concentration of 60, 80 &
100 mg/ml compared to standard drug. The result shows that ethyl ether extract possesses potent vermicidal
activity and found to be effective as an anthelmintic compared to ethanolic extract
The Central and Peripheral effects of the methanol extract of Fadogia cienkow...iosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Hepato Protective Assessment of Pawpaw Leaves, Neem, Lemon Grass and Acts on ...ijtsrd
Malaria is a major concern in Nigeria, and stands as the second leading cause of death from all infectious disease in Africa. Several studies have reported the damaging effect of the parasite to various body organs especially the liver. Reports over time has shown the benefits of various plants extracts in ethno medicine. However, not much have been done on the effects of some of these extracts in combined form on its hepato protective assessment in comparison with any known ACT based anti malaria. The focus of this study was to explore the hepato protective properties of ethanoic extract of Carica papaya Linn, AzadirachtaIndica, CymbopogonCitratusagainst ACT based antimalarial therapy on plasmodium berghei parasitized wistar rats. Phytochemical analysis of the extracts were done according to the method described by Treaseand Evans. Hepato protective assessment were done using the liver function tests and assay of the liver histology respectively. One hundred and ten 110 rats distributed into 11 groups, each group having 10rats were used for the experiment. Negative control received just feed and water, Positive control were induced with the malaria parasite and given feed and water only. The tests groups were induced with malaria, received feed and water and treated with 500mg kg, 250mg kg and 165mg kg doses of the extracts, both individually and in combined forms, as well as the standard ACT anti malaria. Phytochemical screening showed that the plant extracts possessed high concentration of Tannins, Flavonoids, Saponins and Alkaloids. Plasmodium berghei increased the activities of ALP, ASP and ALT when compared with the positive control group. This may be attributed to increase in functional capacity of the liver as a result of the presence of the infection for the tests groups. Treatment with the plant extracts decreased ALP and ALT levels significantly P 0.05 , as well as AST levels except for the Neem extract. Histological examination of the liver of test animals showed no extensive damage to the tissue by the individual extracts when compared to the negative control group. Nnyaha Anthonia E. | Igbokwe Ugochukwu V. | Okonkwo Onyeka Chukwudi | Ajeka Prisca O. | Nwaissac Ikechukwu S. | Okpa Precious N. "Hepato-Protective Assessment of Pawpaw Leaves, Neem, Lemon Grass and Acts on Plasmodium Berghei Parasitized Wistar Rats" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd46292.pdf Paper URL: https://www.ijtsrd.com/medicine/physiology/46292/hepatoprotective-assessment-of-pawpaw-leaves-neem-lemon-grass-and-acts-on-plasmodium-berghei-parasitized-wistar-rats/nnyaha-anthonia-e
— Post-harvest diseases are a major problem for banana yield. Despite treatments with chemical fungicides, a persistence of diseases is noticed. This study aims at proposing a biological control method against banana post-harvest diseases by using banana rachis leachate. The effect of leachate has been tested in vitro on mycelial growth, conidial germination and in vivo on pathogenic fungi virulence. All leachate concentrations (5, 15 and 20%) tested showed antifungal activity on the tested fungi. However, the 20% concentration was more effective with complete inhibition of mycelial growth and conidial germination of all fungi. No symptoms of crown rot and anthracnose were observed after treatment of bananas with leachate. However, with azoxystrobin, the prevalence of crown rot and anthracnose was 60% and 30%, respectively. Banana rachis leachate recorded highly significant reduction of banana finger rot prevalence compared to azoxystrobin. Banana rachis leachate have strong antifungal properties that may be useful to control banana post-harvest disease as a safe alternative option to chemical fungicides Keywords— banana; post-harvest diseases; banana rachis leachate, antifungal activity.
The International Journal of Engineering & Science is aimed at providing a platform for researchers, engineers, scientists, or educators to publish their original research results, to exchange new ideas, to disseminate information in innovative designs, engineering experiences and technological skills. It is also the Journal's objective to promote engineering and technology education. All papers submitted to the Journal will be blind peer-reviewed. Only original articles will be published.
Invasive Alien Plants: Valuable Elixir with Pharmacological and Ethnomedicina...ijtsrd
Use of herbal medicines is propagating day-by-day and several tribes still rely upon this green treasure against their ailments. Being unfortunate to the environment, invasive plants species hold supreme remedies that are unique. Besides ethnoremedial uses they embrace anticancerous, antidiabetic, antimicrobial, antitubercular and other pharmacological attributes in them. In the present review, authors aimed to compile the segregated ethnomedicinal information of invasive plant species. The literature study revealed a significant ethnoremedial importance of invasive alien weeds that may serve to establish a ground for future researchers to explore in pharmacognostic field with safe and natural drug resource. Shaiphali Saxena | P. B. Rao"Invasive Alien Plants: Valuable Elixir with Pharmacological and Ethnomedicinal Attributes" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-3 , April 2018, URL: http://www.ijtsrd.com/papers/ijtsrd11636.pdf http://www.ijtsrd.com/pharmacy/pharmacognosy-/11636/invasive-alien-plants-valuable-elixir-with-pharmacological-and-ethnomedicinal-attributes/shaiphali-saxena
Ethnobotanical uses of wild flora and fungi on the circum-Sicilian island of ...Cassandra Quave
This is a presentation given at the 2015 meeting of the Society for Economic Botany.
Abstract:
In 1969, Galt and Galt conducted an ethnobotanical survey [1] in the community of Khamma on the circum-Sicilian volcanic island of Pantelleria, located 67 km from the Tunisian coast. Since then, a number of botanical studies concerning the local wild flora and cultivation of the zibebo grape and capers have been conducted, but none have investigated traditional ecological knowledge regarding the use of wild plants and fungi. Here, 45 years later, we revisit this topic, examining the current day knowledge and practices concerning wild plants and fungi on the island. A total of 42 in-depth interviews were conducted in June 2014 in six communities. All interviews were conducted in person in Italian with prior informed consent. We employed two primary means of eliciting responses concerning traditional practices; informants were asked to: 1) free-list the most commonly used plants for wild foods, general medicine, and skin remedies; and 2) view and discuss a booklet composed of photos of taxa listed in the Galt study.
A total of 86 botanical and 19 fungal taxa representing 54 families were cited by the study participants. While many plant-based traditions have disappeared from daily practice, especially those related to traditional fishing and hunting, they remain in the memories of the eldest subset of the population. For example, one of the most pervasive species in the landscape, Opuntia ficus-indica, has current day uses that persist (e.g., food source and shade source for cultivated vegetables), but its past applications were much more diverse, and included manipulation into hunting snares for birds. Other predominant flora included a number of Euphorbia spp., whose toxic latex was regularly used as a fish poison. Fungi, on the other hand, represent an important source of wild food today, and are widely recognized and regularly consumed. In conclusion, we have found that traditional e knowledge remains important to everyday practices of Pantescans, ranging in applications from wild foods, agricultural tools, and traditional medicines.
in vitro screening of larvicidal and insecticidal activity of methanolic extr...IJEAB
The aim of this work was to evaluate the larvicidal and insecticidal activity of the selected plants namelyArtocarpus altilis, Piper betle and Artocarpus heterophyllus. The leaves of Artocarpus altilis and Artocarpus heterophyllus and roots of Piper betle were subjected to methanolic solvent extraction for the isolation of various bioactive constituents. The evaluation of larvicidal activity was carried out using late third instar larvae of Drosophila melanogaster. The insecticidal activity of extracts was studied against adult Bruchus pisorum, Tribolium castaneum, Sitophilus oryzae and was evaluated by direct contact application method .Nucleic acids and protein contents are regarded as important biomarkers of the metabolic potential of cells, as these play the main role in regulating the different activities of cells. Piper betle and Artocarpus heterophyllusextractshad a reducing effect on the nucleic acid and protein content in the larvae in a dose dependent manner whereas Artocarpus altilis extract did not exhibit any significant larvicidal activity. Piper betle and Artocarpus heterophyllusextractsshowed good insecticidal activity whereas A.altilis extract showed poor insecticidal activity. The results of the present study clearly indicate that Piper betle and Artocarpus heterophyllus extracts can be developed as ecofriendly larvicides and were also quite effective as insecticides for providing a better and excellent alternate for the control of insects.
Atlanta Botanical Garden Science Cafe: Medicines from Nature - 2014Cassandra Quave
In 2014, Dr. Quave presented a Science Cafe talk at Atlanta Botanical Gardens: "Medicines From Nature: Adventures of a Medical Ethnobotanist"
Abstract:
Ethnobotany is the study of human interactions with plants. It has perhaps been best defined as the science of survival as it involves the study of plants used in our daily lives for food, medicine, shelter, art, and much more. In this science café, Dr. Quave will discuss her research on medicinal plants for the treatment of infectious disease. From remote regions of the Amazon to volcanic islands in the Mediterranean and isolated mountains in the Balkans, Quave has conducted fieldwork in some of the most biologically diverse regions of the planet. She will share some of her adventures as a medical ethnobotanist and explain how traditional knowledge may be the key to the discovery of new life-saving medicines.
Effect of the Gayatri Mantra Playing on Microbial Load in Room AirBhoj Raj Singh
In the study, the effect of the Gayatri Mantra playing for 24 hr on microbial quality of air was examined in 12 rooms of scientists of the ICAR-Indian Veterinary Research Institute, Izatnagar, India willing to participate in the study and in 7 empty classroom/ examination hall lying vacant since March 2020 due to COVID-19. Of the empty rooms, 6 were used as the control for the first two days, then after a week, all rooms were also used to detect the effect of playing the Gayatri Mantra. A total of 31 bacterial species of medical importance were isolated and identified in the air of the rooms. After playing the Gayatri Mantra in Raag Bhairvi for 24 h bacterial count significantly decreased in rooms inhabited by vegetarian (OR 24, CI 95: 0.00-0.88; p, 0.036) than in rooms occupied by non-vegetarians. Rooms occupied by vegetarians. The effect of Gayatri Mantra in the empty room had no significant difference but bacterial count reduced. Bacterial counts of rooms occupied by non-vegetarian increased significantly post Mantra playing. Bacterial counts of rooms occupied by non-vegetarians and empty rooms varied significantly (OR 18, p, 0.02, CI 95: 0.00 – 0.79). Empty rooms with and without mantra not varied significantly for the reduction of the bacterial count. Paenibacillus spp. was not detected in any of the 12 rooms occupied by the scientists/ staff but in 3 of the 7 empty rooms (p, 0.01) it was detected even without playing any mantra. It indicated that daily disturbance in the environment may be detrimental to the survival of Paenibacillus spp. After playing Mantra P. alvei, P. cookie, P. lautus were detected in 5 of the seven rooms occupied by Non-vegetarians, while P. pantothenicus continued to be present in three of the vacant rooms even after playing the Mantra. Paenibacillus spp. bacteria are known for their probiotic potential and its significance in the study is not clear. It seems that the Gayatri mantra has some enrichment effect on Paenibacillus spp. but a detrimental effect on other microbes.
Microbiological Investigations of Selected Flies of Public Health Importance ...iosrjce
Bacteria associated with flies of public health importance in Nigeria are not well known and their
ecology is also not well understood. We aim to determine the bacteria associated with flies of waste dump site.
Three flies of public health significance were collected from a waste dump site of the Rivers State University of
Science and Technology, Port Harcourt. The three dipterous flies were Luciliasericata, Chrysomyasp and
Musca domestica..The three flies were all of medical importance.The microbial load on three species of flies
was investigated using standard plate count methods. The fly samples were collected from the Post Graduate
Entomology Laboratory was cultured to isolate and identify the microbes associated with them. The samples
were analyzed for total heterotrophic bacteria and fungi counts. The study revealed high heterotrophic bacteria
and fungi counts in all three species of the flies used, with Musca domestica having the highest count of 2.9 X
109Cfu/gram and Chrysomyasp with the least count of 3.4 x 10 5Cfu/g and fungi counts ranged from 3.1 X
103Cfu/g to 2.9 X 105Cfu/g. The bacteria isolated from these samples includes: Escherichia coli,
Pseudomonassp,, Bacillussp, Enterobactersp, Staphylococcussp,Salmonellasp, Proteussp, and Klebsiellasp,
while the fungi species isolated includes: Penicilliumsp,
Aspergillussp,Rhizopussp,Cladosporiumsp,Aspergillusflavus, Aspergillusniger, Fusariumsp and Trichoderma
sp.
Analyses of Bacterial Community Dynamics Present in Culex quinquefasciatus Co...BRNSS Publication Hub
Culex quinquefasciatus are among the most important vectors of arboviral diseases worldwide. Recent
studies indicate that diverse midgut microbiota of mosquitoes significantly affects development, digestion,
metabolism, and immunity of their hosts. Here, we studied the bacterial diversity found in midgut part
of C. quinquefasciatus to understand the host and microbe interaction. The adult C. quinquefasciatus
mosquitos were collected from Loyola College Campus, Chennai, using ovitraps, and midgut part was
extracted; moreover, the DNA templates were isolated and amplified by polymerase chain reaction. The
DNA amplicons were sequenced by Illumina MiSeq gene sequencer. The total of 279,157 reads was
classified into 85, the bacterial genera of Pseudomonas, Klebsiella, Staphylococcus, and Aeromonas
predominantly found to be high when compared to the other bacterial genera. The present data strongly
encourage further investigations to verify the potential role of the detected bacteria in mosquito for the
transmission of several vectoral diseases.
Mosquitocidal property of leaf extract of bougainvillea spectabilis (Nictagin...researchanimalsciences
Mosquitocidal property of leaf extract of Bougainvillea spectabilis was evaluated for the egg hatchability, larvicidal and pupicidal activity of mosquito, Aedes aegypti under the room temperature in the laboratory. A relationship was observed between the plant extract dose and the percentage of egg hatchability, larval and pupal mortality. Dosage value as expressed in % was 0.01 to 4.0 for Aedes aegypti. The percentage of egg hatchability, larval and pupal mortality were found to increase with the dosage indicating a relationship between the two. Based on the probit analysis the LC50 (mg/l) value of egg (31), I instar (59), II instar (231), III instar (606), IV instar (1578) and pupa (2637) were observed.
Article Citation:
Rajmohan D and Logankumar K.
Mosquitocidal property of leaf extract of Bougainvillea spectabilis (Nictaginaceae) against the growth and development of the mosquito, Aedes aegypti. l.
(Diptera: Culicidae)
Journal of Research in Animal Sciences (2012) 1(1): 028-032.
Full Text:
http://janimalsciences.com/documents/AS0012.pdf
Microbiological Investigations on Gryllotalpa Africana [Orthoptera: Gryllotal...iosrjce
This paper reports for the first time the bacterial distribution on Gryllotalpa africana, a mole cricket
and one of the edible hexapods of the Niger Delta region of Nigeria. Children hunt it during the rainy and also
at dry seasons and it is harvested as snacks in the rural settings of the region. It is eaten raw, boiled, fried or
roasted along with other condiments including onions and pepper. It is a delicacy enjoyed in many other parts
of Nigeria. Assessments were made to identify bacteria that are associated with different external structures of
the edible mole cricket.
Mosquito larvicidal activity of leaf and seed extracts of Lantana camara on A...researchanimalsciences
Background and Objectives: This paper reports the toxicity of Lantana camara to developmental stages of the yellow fever mosquito, Aedes aegypti. Aqueous extracts of leaf and seed of the plant were also tested for their effect on the hatchability of mosquito egg and age at pupation and emergence.
Methods: Different concentrations of aqueous leaf and seed extract were prepared. The data of mortality rate were subjected to finney’s method of probit analysis. The plant was also tested for their effect on the hatchability of mosquito eggs.
Results: Percent log LC50 / 24 h values of the leaf and seed extracts of L. camara to IV instar larvae were 2.25 and 2.25 respectively. Percent hatchability of mosquito eggs was remarkably reduced when treated with higher concentration of the toxicants. Extended time of pupation and emergence was observed for the larvae reared in different concentrations of the plant extract.
Conclusion: The results suggested that leaf and seed extract of Lantana camera possessed remarkable larvicidal, ovicidal, and prolonged time of pupation and adult emergence against Aedes aegypti.
Article Citation:
Sathya K, Mohanraj RS, Dhanakkodi B .
Mosquito larvicidal activity of leaf and seed extracts of Lantana camara on Aedes aegypti.
Journal of Research in Animal Sciences (2012) 1(2): 040-047.
Full Text:
http://janimalsciences.com/documents/AS0013.pdf
The Effect of Dried Leaves Extract of Hyptis suaveolens on Various Stages of ...iosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Evaluation of the Antibacterial Activities of Crude Extracts of Cochlospermum...iosrjce
The antibacterial activities of the methanolic, hot water, chloroform and petroleum ether extracts of
Choclospermum planchonii leaf were tested against some clinical bacterial isolates and reference organisms.
The phytochemical screenings of the extracts were also carried out. The leaf extracts of the plant exhibited
antibacterial activities against clinical isolates of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus
aureus, Shigella flexneri, and Salmonella typhii and reference strains of Staphylococcus aureus (ATCC 28923),
Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853. The methanolic leaf extracts
exhibited the highest antibacterial activity, its minimum inhibitory concentration (MIC) ranging between 0.31
and 1.25 mg/ml; and its zones of inhibition on the various test microorganisms ranging between 8mm and 13
mm. The petroleum ether extracts had the weakest antibacterial activity, its minimum inhibitory concentration
(MIC) ranging between 2.50mg/ml and 5.00mg/ml and its inhibition zone ranging between 4mm and 8mm. The
phytochemical constituents present in the plant were alkaloids, tannins, saponins, cardiac glycosides, and
sterols. The methanolic extracts of the leaf exhibited potentially biological activity than other extracts. This
indicates that the plant leaf could be useful in the treatment of human infections caused by these pathogens. The
antibacterial activities of the methanolic leaf extract may be attributed to the presence of bioactive compounds.
The International Journal of Engineering & Science is aimed at providing a platform for researchers, engineers, scientists, or educators to publish their original research results, to exchange new ideas, to disseminate information in innovative designs, engineering experiences and technological skills. It is also the Journal's objective to promote engineering and technology education. All papers submitted to the Journal will be blind peer-reviewed. Only original articles will be published.
Invasive Alien Plants: Valuable Elixir with Pharmacological and Ethnomedicina...ijtsrd
Use of herbal medicines is propagating day-by-day and several tribes still rely upon this green treasure against their ailments. Being unfortunate to the environment, invasive plants species hold supreme remedies that are unique. Besides ethnoremedial uses they embrace anticancerous, antidiabetic, antimicrobial, antitubercular and other pharmacological attributes in them. In the present review, authors aimed to compile the segregated ethnomedicinal information of invasive plant species. The literature study revealed a significant ethnoremedial importance of invasive alien weeds that may serve to establish a ground for future researchers to explore in pharmacognostic field with safe and natural drug resource. Shaiphali Saxena | P. B. Rao"Invasive Alien Plants: Valuable Elixir with Pharmacological and Ethnomedicinal Attributes" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-3 , April 2018, URL: http://www.ijtsrd.com/papers/ijtsrd11636.pdf http://www.ijtsrd.com/pharmacy/pharmacognosy-/11636/invasive-alien-plants-valuable-elixir-with-pharmacological-and-ethnomedicinal-attributes/shaiphali-saxena
Ethnobotanical uses of wild flora and fungi on the circum-Sicilian island of ...Cassandra Quave
This is a presentation given at the 2015 meeting of the Society for Economic Botany.
Abstract:
In 1969, Galt and Galt conducted an ethnobotanical survey [1] in the community of Khamma on the circum-Sicilian volcanic island of Pantelleria, located 67 km from the Tunisian coast. Since then, a number of botanical studies concerning the local wild flora and cultivation of the zibebo grape and capers have been conducted, but none have investigated traditional ecological knowledge regarding the use of wild plants and fungi. Here, 45 years later, we revisit this topic, examining the current day knowledge and practices concerning wild plants and fungi on the island. A total of 42 in-depth interviews were conducted in June 2014 in six communities. All interviews were conducted in person in Italian with prior informed consent. We employed two primary means of eliciting responses concerning traditional practices; informants were asked to: 1) free-list the most commonly used plants for wild foods, general medicine, and skin remedies; and 2) view and discuss a booklet composed of photos of taxa listed in the Galt study.
A total of 86 botanical and 19 fungal taxa representing 54 families were cited by the study participants. While many plant-based traditions have disappeared from daily practice, especially those related to traditional fishing and hunting, they remain in the memories of the eldest subset of the population. For example, one of the most pervasive species in the landscape, Opuntia ficus-indica, has current day uses that persist (e.g., food source and shade source for cultivated vegetables), but its past applications were much more diverse, and included manipulation into hunting snares for birds. Other predominant flora included a number of Euphorbia spp., whose toxic latex was regularly used as a fish poison. Fungi, on the other hand, represent an important source of wild food today, and are widely recognized and regularly consumed. In conclusion, we have found that traditional e knowledge remains important to everyday practices of Pantescans, ranging in applications from wild foods, agricultural tools, and traditional medicines.
in vitro screening of larvicidal and insecticidal activity of methanolic extr...IJEAB
The aim of this work was to evaluate the larvicidal and insecticidal activity of the selected plants namelyArtocarpus altilis, Piper betle and Artocarpus heterophyllus. The leaves of Artocarpus altilis and Artocarpus heterophyllus and roots of Piper betle were subjected to methanolic solvent extraction for the isolation of various bioactive constituents. The evaluation of larvicidal activity was carried out using late third instar larvae of Drosophila melanogaster. The insecticidal activity of extracts was studied against adult Bruchus pisorum, Tribolium castaneum, Sitophilus oryzae and was evaluated by direct contact application method .Nucleic acids and protein contents are regarded as important biomarkers of the metabolic potential of cells, as these play the main role in regulating the different activities of cells. Piper betle and Artocarpus heterophyllusextractshad a reducing effect on the nucleic acid and protein content in the larvae in a dose dependent manner whereas Artocarpus altilis extract did not exhibit any significant larvicidal activity. Piper betle and Artocarpus heterophyllusextractsshowed good insecticidal activity whereas A.altilis extract showed poor insecticidal activity. The results of the present study clearly indicate that Piper betle and Artocarpus heterophyllus extracts can be developed as ecofriendly larvicides and were also quite effective as insecticides for providing a better and excellent alternate for the control of insects.
Atlanta Botanical Garden Science Cafe: Medicines from Nature - 2014Cassandra Quave
In 2014, Dr. Quave presented a Science Cafe talk at Atlanta Botanical Gardens: "Medicines From Nature: Adventures of a Medical Ethnobotanist"
Abstract:
Ethnobotany is the study of human interactions with plants. It has perhaps been best defined as the science of survival as it involves the study of plants used in our daily lives for food, medicine, shelter, art, and much more. In this science café, Dr. Quave will discuss her research on medicinal plants for the treatment of infectious disease. From remote regions of the Amazon to volcanic islands in the Mediterranean and isolated mountains in the Balkans, Quave has conducted fieldwork in some of the most biologically diverse regions of the planet. She will share some of her adventures as a medical ethnobotanist and explain how traditional knowledge may be the key to the discovery of new life-saving medicines.
Effect of the Gayatri Mantra Playing on Microbial Load in Room AirBhoj Raj Singh
In the study, the effect of the Gayatri Mantra playing for 24 hr on microbial quality of air was examined in 12 rooms of scientists of the ICAR-Indian Veterinary Research Institute, Izatnagar, India willing to participate in the study and in 7 empty classroom/ examination hall lying vacant since March 2020 due to COVID-19. Of the empty rooms, 6 were used as the control for the first two days, then after a week, all rooms were also used to detect the effect of playing the Gayatri Mantra. A total of 31 bacterial species of medical importance were isolated and identified in the air of the rooms. After playing the Gayatri Mantra in Raag Bhairvi for 24 h bacterial count significantly decreased in rooms inhabited by vegetarian (OR 24, CI 95: 0.00-0.88; p, 0.036) than in rooms occupied by non-vegetarians. Rooms occupied by vegetarians. The effect of Gayatri Mantra in the empty room had no significant difference but bacterial count reduced. Bacterial counts of rooms occupied by non-vegetarian increased significantly post Mantra playing. Bacterial counts of rooms occupied by non-vegetarians and empty rooms varied significantly (OR 18, p, 0.02, CI 95: 0.00 – 0.79). Empty rooms with and without mantra not varied significantly for the reduction of the bacterial count. Paenibacillus spp. was not detected in any of the 12 rooms occupied by the scientists/ staff but in 3 of the 7 empty rooms (p, 0.01) it was detected even without playing any mantra. It indicated that daily disturbance in the environment may be detrimental to the survival of Paenibacillus spp. After playing Mantra P. alvei, P. cookie, P. lautus were detected in 5 of the seven rooms occupied by Non-vegetarians, while P. pantothenicus continued to be present in three of the vacant rooms even after playing the Mantra. Paenibacillus spp. bacteria are known for their probiotic potential and its significance in the study is not clear. It seems that the Gayatri mantra has some enrichment effect on Paenibacillus spp. but a detrimental effect on other microbes.
Microbiological Investigations of Selected Flies of Public Health Importance ...iosrjce
Bacteria associated with flies of public health importance in Nigeria are not well known and their
ecology is also not well understood. We aim to determine the bacteria associated with flies of waste dump site.
Three flies of public health significance were collected from a waste dump site of the Rivers State University of
Science and Technology, Port Harcourt. The three dipterous flies were Luciliasericata, Chrysomyasp and
Musca domestica..The three flies were all of medical importance.The microbial load on three species of flies
was investigated using standard plate count methods. The fly samples were collected from the Post Graduate
Entomology Laboratory was cultured to isolate and identify the microbes associated with them. The samples
were analyzed for total heterotrophic bacteria and fungi counts. The study revealed high heterotrophic bacteria
and fungi counts in all three species of the flies used, with Musca domestica having the highest count of 2.9 X
109Cfu/gram and Chrysomyasp with the least count of 3.4 x 10 5Cfu/g and fungi counts ranged from 3.1 X
103Cfu/g to 2.9 X 105Cfu/g. The bacteria isolated from these samples includes: Escherichia coli,
Pseudomonassp,, Bacillussp, Enterobactersp, Staphylococcussp,Salmonellasp, Proteussp, and Klebsiellasp,
while the fungi species isolated includes: Penicilliumsp,
Aspergillussp,Rhizopussp,Cladosporiumsp,Aspergillusflavus, Aspergillusniger, Fusariumsp and Trichoderma
sp.
Analyses of Bacterial Community Dynamics Present in Culex quinquefasciatus Co...BRNSS Publication Hub
Culex quinquefasciatus are among the most important vectors of arboviral diseases worldwide. Recent
studies indicate that diverse midgut microbiota of mosquitoes significantly affects development, digestion,
metabolism, and immunity of their hosts. Here, we studied the bacterial diversity found in midgut part
of C. quinquefasciatus to understand the host and microbe interaction. The adult C. quinquefasciatus
mosquitos were collected from Loyola College Campus, Chennai, using ovitraps, and midgut part was
extracted; moreover, the DNA templates were isolated and amplified by polymerase chain reaction. The
DNA amplicons were sequenced by Illumina MiSeq gene sequencer. The total of 279,157 reads was
classified into 85, the bacterial genera of Pseudomonas, Klebsiella, Staphylococcus, and Aeromonas
predominantly found to be high when compared to the other bacterial genera. The present data strongly
encourage further investigations to verify the potential role of the detected bacteria in mosquito for the
transmission of several vectoral diseases.
Mosquitocidal property of leaf extract of bougainvillea spectabilis (Nictagin...researchanimalsciences
Mosquitocidal property of leaf extract of Bougainvillea spectabilis was evaluated for the egg hatchability, larvicidal and pupicidal activity of mosquito, Aedes aegypti under the room temperature in the laboratory. A relationship was observed between the plant extract dose and the percentage of egg hatchability, larval and pupal mortality. Dosage value as expressed in % was 0.01 to 4.0 for Aedes aegypti. The percentage of egg hatchability, larval and pupal mortality were found to increase with the dosage indicating a relationship between the two. Based on the probit analysis the LC50 (mg/l) value of egg (31), I instar (59), II instar (231), III instar (606), IV instar (1578) and pupa (2637) were observed.
Article Citation:
Rajmohan D and Logankumar K.
Mosquitocidal property of leaf extract of Bougainvillea spectabilis (Nictaginaceae) against the growth and development of the mosquito, Aedes aegypti. l.
(Diptera: Culicidae)
Journal of Research in Animal Sciences (2012) 1(1): 028-032.
Full Text:
http://janimalsciences.com/documents/AS0012.pdf
Microbiological Investigations on Gryllotalpa Africana [Orthoptera: Gryllotal...iosrjce
This paper reports for the first time the bacterial distribution on Gryllotalpa africana, a mole cricket
and one of the edible hexapods of the Niger Delta region of Nigeria. Children hunt it during the rainy and also
at dry seasons and it is harvested as snacks in the rural settings of the region. It is eaten raw, boiled, fried or
roasted along with other condiments including onions and pepper. It is a delicacy enjoyed in many other parts
of Nigeria. Assessments were made to identify bacteria that are associated with different external structures of
the edible mole cricket.
Mosquito larvicidal activity of leaf and seed extracts of Lantana camara on A...researchanimalsciences
Background and Objectives: This paper reports the toxicity of Lantana camara to developmental stages of the yellow fever mosquito, Aedes aegypti. Aqueous extracts of leaf and seed of the plant were also tested for their effect on the hatchability of mosquito egg and age at pupation and emergence.
Methods: Different concentrations of aqueous leaf and seed extract were prepared. The data of mortality rate were subjected to finney’s method of probit analysis. The plant was also tested for their effect on the hatchability of mosquito eggs.
Results: Percent log LC50 / 24 h values of the leaf and seed extracts of L. camara to IV instar larvae were 2.25 and 2.25 respectively. Percent hatchability of mosquito eggs was remarkably reduced when treated with higher concentration of the toxicants. Extended time of pupation and emergence was observed for the larvae reared in different concentrations of the plant extract.
Conclusion: The results suggested that leaf and seed extract of Lantana camera possessed remarkable larvicidal, ovicidal, and prolonged time of pupation and adult emergence against Aedes aegypti.
Article Citation:
Sathya K, Mohanraj RS, Dhanakkodi B .
Mosquito larvicidal activity of leaf and seed extracts of Lantana camara on Aedes aegypti.
Journal of Research in Animal Sciences (2012) 1(2): 040-047.
Full Text:
http://janimalsciences.com/documents/AS0013.pdf
The Effect of Dried Leaves Extract of Hyptis suaveolens on Various Stages of ...iosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Evaluation of the Antibacterial Activities of Crude Extracts of Cochlospermum...iosrjce
The antibacterial activities of the methanolic, hot water, chloroform and petroleum ether extracts of
Choclospermum planchonii leaf were tested against some clinical bacterial isolates and reference organisms.
The phytochemical screenings of the extracts were also carried out. The leaf extracts of the plant exhibited
antibacterial activities against clinical isolates of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus
aureus, Shigella flexneri, and Salmonella typhii and reference strains of Staphylococcus aureus (ATCC 28923),
Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853. The methanolic leaf extracts
exhibited the highest antibacterial activity, its minimum inhibitory concentration (MIC) ranging between 0.31
and 1.25 mg/ml; and its zones of inhibition on the various test microorganisms ranging between 8mm and 13
mm. The petroleum ether extracts had the weakest antibacterial activity, its minimum inhibitory concentration
(MIC) ranging between 2.50mg/ml and 5.00mg/ml and its inhibition zone ranging between 4mm and 8mm. The
phytochemical constituents present in the plant were alkaloids, tannins, saponins, cardiac glycosides, and
sterols. The methanolic extracts of the leaf exhibited potentially biological activity than other extracts. This
indicates that the plant leaf could be useful in the treatment of human infections caused by these pathogens. The
antibacterial activities of the methanolic leaf extract may be attributed to the presence of bioactive compounds.
Invitro and Invivo antibacterial study of leaf extracts of Euphorbia heteroph...Premier Publishers
A study was carried out to determine the phytochemical components and antibacterial activity of Euphorbia heterophylla crude extracts on four enteric organisms namely; Salmonella typhi, Shigella flexneri, Escherichia coli and Proteus vulgaris. The clinical isolates of Salmonella typhi, Shigella flexneri, E.coli and Proteus vulgaris were subjected to antimicrobial susceptibility test using agar diffusion technique. Phytochemistry of the Euphorbia heterophylla, crude extracts revealed the presence of flavonoids, alkaloids, saponins and cardiac glycosides. Methanolic and aqueous crude extract produced clear zones of inhibition at concentration ranging from 50 to 200mg/ml. In vivo antimicrobial assay revealed that the mice treated with the crude methanolic and aqueous extracts after being infected with the various test organisms, survived and showed minute pathological effects. Similarly, untreated mice (control) died after 48hours of inoculation with Salmonella typhi, Shigella flexneri, E.coli and Proteus vulgaris. Euphorbia heterophylla crude extract, most especially its methanolic and aqueous counterpart could be a potential source for the treatment of diseases associated with enteric organisms. Further studies should be directed towards isolation and characterization of the active compound in the crude extracts.
SYNERGISTIC ANTIMICROBIAL ACTIVITIES OF PHYTOESTROGENS IN CRUDE EXTRACTS OF T...lukeman Joseph Ade shittu
Intensive studies on extracts and biologically active compounds isolated from medicinal plants have doubled in the last decade worldwide. However, as a result of paucity of knowledge and folkloric claim on the effectiveness of sesame leaves in infectious disease treatments, we aimed to determine the synergistic antimicrobial activity of essential oils and lignans present in the crude leaves extracts of Sesame radiatum and Sesame indicum. Ethanolic, methanolic and aqueous extracts of both leaves were studied for their in-vitro synergistic antimicrobial activity against both Gram positive and Gram negative micro-organisms, and Yeast using Agar diffusion method. The GC-MS phytochemical screening of methanolic extract showed that the major compounds in essential oils are of carboxylic acids and phenolic groups especially, the most potent antioxidants known to man like sesamol, sesamolin and sesamin among others. Methanolic and ethanolic extracts have broad spectrum antimicrobial effect against all the tested pathogenic micro-organisms except Streptococcus pneumoniae and Staphylococcus aureus respectively, while the aqueous extract exhibited inhibitory activity on Staphylococcus aureus, Streptococcus pneumoniae and Candida albicans. The result confirmed the folkloric claims of the antimicrobial effectiveness of locally consumed sesame leaves extracts especially against bacterial and common skin infection in many areas of Nigeria .
Antibiogram of Staphylococcus Aureus and its Sensitivity to Ocimum Gratissimu...ijtsrd
In this study, clinical isolate of Staphylococcus aureus was subjected to susceptibility tests against commercially available antibiotics and Ocimum gratissimum scent leaf leaf extracts. disc diffusion nmethod was adopted for the antibiotics test while agar well diffusion technique was employed for the antimicrobial screening of the leaf extracts. The result shows that the organism was sensitive to Gentamycin, Zinnacef, Ciprofloxacin and Streptomycin representing 40 of the antibiotics while showing outright resistance to Ampiclox, Amoxicillin and Erythromycin 30 and intermediate results for the rest. Also, the cold water extract of O. gratissimum did not show any activity against the bacterium. However, both hot water extract and ethanol extract of the plant had varying degree of activity against the organism with ethanol extract recording a zone of inhibition of 25.33 mm compared with 32.00 mm recorded for Gentamycin. Furthermore, the minimum inhibitory concentration recorded ofr the extract against the organism were 10 mg ml and 2.5 mg ml respectively for hot water and ethanol extracts. These results indicate that ethanol extract of O. gratissimum leaf is very effective against Staphylococcus aureus and could be used as potential source of natural product for the treatment of infections caused by the organism especially the antibiotic resistant strains. Komolafe T. O. | Ogunyankin O. G "Antibiogram of Staphylococcus Aureus and its Sensitivity to Ocimum Gratissimum Extract" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, URL: https://www.ijtsrd.com/papers/ijtsrd46333.pdf Paper URL : https://www.ijtsrd.com/other-scientific-research-area/other/46333/antibiogram-of-staphylococcus-aureus-and-its-sensitivity-to-ocimum-gratissimum-extract/komolafe-t-o
Insecticidal activities of diketopiperazines of Nomuraea rileyi entomopathoge...IJEAB
Entomopathogenic fungi are fungal organisms extensively used in various parts of the world as biopesticides against insect pests that cause important economic damage. Various secondary metabolites produced by these fungi have many potential biological activities. The present study was undertaken to evaluate the insecticidal activity of extracts and pure compounds from Nomuraea rileyi (Farlow) Samson entomopathogenic fungi against Spodoptera frugiperda Smith (Lepidoptera), Ceratitis capitata Wiedemann (Diptera) and Tribolium castaneum Herbst (Coleoptera), three insect pests that generate serious economic losses in the northwest of Argentina. Diketopiperazines were extracted from the culture free supernatant of the media with ethyl acetate. Antifeedant properties were detected in all extracts under dietary choice conditions (300 ug/ g of diet). The maximum antifeedant activity was noted in cycles (Pro-Val) (86.02) and cycle (Pro-Phe) (73.47), while the rest of the extracts and metabolites exhibited varying degrees of moderate or less toxic effects. The maximum oviposition deterrence against C. capitata (55.86%) was recorded with cycle (Pro-Phe) at a 50 µm/cm2 dose. Culture medium extracts supplemented with insect remains and all pure compounds showed repellent action against T. castaneum. The main repellency was observed in phenylacetic acid and cycle (Pro-Val) with RI values of 42 and 41% respectively. The present study would suggest the possible utilization of entomopathogenic fungal metabolites as an effective agent for controlling insect pests that cause important economic losses.
ABSTRACT- Mosquito-borne diseases have intruded the globe since immemorial time. The present scenario for
commanding the mosquitoes is aimed at application of target and stage-specific, cost-effective and biodegradable
phytoproducts. Plant extracts are safer for non-target organisms including man. Plant based formulations would be more
feasible environmental products with proven potential as insecticide. Therefore, in the present study of larvicidal
activity of biologically active compound Apigenin extracted from leaf of Jatropha gossypifolia against the filarial
vector, Culex quinquefasciatus was studied. Standard WHO protocols with minor modifications was adopted for the
larvicidal bioassay. The active compound Apigenin extracted through ethyl alcohol solvent from the leaf of Jatropha
gossypifolia plant of family Euphorbiaceae was administered for 24h or 96h to the larvae of Culex quinquefasciatus.
Exposure of larvae over 24h to sub-lethal doses (40% and 80% of LC50) of apigenin, significantly (P<0.05) altered the
level of total protein, total free amino acid, glycogen and activity of enzymes acetyl cholinesterase, acid and alkaline
phosphatase activity in whole body tissue of Culex quinquefasciatus larvae. The alterations in all these biochemical
parameters were significantly (P<0.05) time and dose dependent.
Key-words- Jatropha gossypifolia, Euphorbiaceae, Culex quinquefasciatus, biochemical effects, Wuchereria bancrofti
The aim of the study was to evaluate the antibacterial evaluation of root extracts of Juglans regia against Extended Spectrum Beta Lactamase (ESBL) producing E. coli and Klebsiella pneumonia in Bombay Hospital and Research Centre Jabalpur. The antibacterial activity of, ethyl acetate and methanol root extracts of Juglans regia was determined by disk diffusion method. The antibacterial activity was calculated based on the minimum inhibitory concentration using Mueller–Hinton broth in a tube-dilution method. The best antibacterial activity, calculated as minimum inhibitory concentration values, against ESBL was shown by the methanol root extract Juglans regia (25 mg/mL) for both isolated organisms and ethyl acetate (25mg/mL) against E. coli. The methanol extract showed zone of inhibition in the range of 17-26mm as compared to ethyl acetate extract which showed zone of inhibition in the range of 11-16mm against the uropathogens. The zone of inhibition ranged from 17 mm to 26 mm and MIC was 25mg/ml. This effect is comparable to antibiotics. The results obtained in the present study suggest that Juglans regia have the potential to be developed as antibacterial agents against ESBL producing UTI bacteria strain. Further investigations are needed to identify the active compounds and their mechanism of action
Study of virulence genes in vancomycin resistant Enterococci (vre) from anima...Innspub Net
With Enterococcus species in the leading cause of nosocomial infections and resistance to an array of antibiotics, this study focused to determine the frequency and distribution of vancomycin-resistant Enterococci, the presence of virulence genes and to determine the relative nucleotide sequence relatedness among isolates using 16S rRNA sequence. A random sampling of 120 fecal samples of cattle, poultry, and piggery, and human clinical isolates was analyzed. Standard bacteriological methods were employed in the isolation and characterization of isolates and the disk diffusion method was used in determining their antibiotic resistance profiles. Results showed Enterococcus species in cattle at 100%, followed by clinical isolates at 80%. Vancomycin resistance was observed at high rates in Enterococcus species from human clinical isolates and cattle isolates at 90% and 80% respectively. Multiple antibiotic-resistant isolates yielded twelve resistance profiles and 16S rDNA sequences identified E. faecalis, E. durans, E. mundtii, and Enterococcus sp. Isolates from cattle samples were the most probable source of clinical isolates at 78% homology of conserved regions with the clinical isolates. Virulence determinant genes Asa1 was recorded at66.6%, Cyl at 16.6% and GelE at 8.3% among the isolates. This study established farm animals as possible reservoirs of VRE isolates to man. Hence, healthy and professional practices among animal farmers with antibiotic usage, as well as hygienic and preventive measures among hospital workers are here recommended.
Molecular Identification of Bulinus Species in Ogun State, South-West Nigeria...AI Publications
The study considers the distribution of a small sample of 100 Bulinus snails, across 8 localities within Ogun State, Nigerian. Snails were identified using a molecular method of fragment and restriction profiles obtained from ribosomal internal transcribed spacer (its) amplicons. The results showed that the majority of Bulinus samples tested belonged to the species Bulinustruncatus while only one was Bulinusglobosus. The use of Rsa1 restriction endonuclease to cleave the ribosomal its of Bulinus, as a method of species identification, was adopted for the majority of samples, this being a quicker and cheaper method better suited to small laboratory environments. Polymerase chain reaction (PCR) amplification of the schistosome Dra1 repeat within each of the collected Bulinus samples was employed to determine the extent and distribution of infected snails within the sample areas. Successful amplification of the Dra1 repeat demonstrated that 23% of snails were infected with schistosome
Studies on Prevalence of Ixodid Ticks Infesting Cattle and Their Control by P...iosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
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RMD24 | Debunking the non-endemic revenue myth Marvin Vacquier Droop | First ...BBPMedia1
Marvin neemt je in deze presentatie mee in de voordelen van non-endemic advertising op retail media netwerken. Hij brengt ook de uitdagingen in beeld die de markt op dit moment heeft op het gebied van retail media voor niet-leveranciers.
Retail media wordt gezien als het nieuwe advertising-medium en ook mediabureaus richten massaal retail media-afdelingen op. Merken die niet in de betreffende winkel liggen staan ook nog niet in de rij om op de retail media netwerken te adverteren. Marvin belicht de uitdagingen die er zijn om echt aansluiting te vinden op die markt van non-endemic advertising.
RMD24 | Retail media: hoe zet je dit in als je geen AH of Unilever bent? Heid...BBPMedia1
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[Note: This is a partial preview. To download this presentation, visit:
https://www.oeconsulting.com.sg/training-presentations]
Sustainability has become an increasingly critical topic as the world recognizes the need to protect our planet and its resources for future generations. Sustainability means meeting our current needs without compromising the ability of future generations to meet theirs. It involves long-term planning and consideration of the consequences of our actions. The goal is to create strategies that ensure the long-term viability of People, Planet, and Profit.
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LEARNING OBJECTIVES
1. Develop a comprehensive understanding of the fundamental principles and concepts that form the foundation of sustainability within corporate environments.
2. Explore the sustainability implementation model, focusing on effective measures and reporting strategies to track and communicate sustainability efforts.
3. Identify and define best practices and critical success factors essential for achieving sustainability goals within organizations.
CONTENTS
1. Introduction and Key Concepts of Sustainability
2. Principles and Practices of Sustainability
3. Measures and Reporting in Sustainability
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To download the complete presentation, visit: https://www.oeconsulting.com.sg/training-presentations
Discover the innovative and creative projects that highlight my journey throu...dylandmeas
Discover the innovative and creative projects that highlight my journey through Full Sail University. Below, you’ll find a collection of my work showcasing my skills and expertise in digital marketing, event planning, and media production.
An investigation of the lethality of picralima nitida, family apocynaceae in malaria vector control
1. Advances in Life Science and Technology www.iiste.org
ISSN 2224-7181 (Paper) ISSN 2225-062X (Online)
Vol.23, 2014
An Investigation of the Lethality of Picralima Nitida, Family
Apocynaceae in Malaria Vector Control
Nwabor, Ozioma Forstinus, Dibua, Uju Marie Esther 1, Nnamonu, Emmanuel Ikechukwu2, Odiachi, Osita1,
Edeh,Ojima Gloria1, Ezechukwu, Chiemekam Samuel2, Eze, Tochukwu Rex3,
Izunna, Stanislaus
1. Department of Microbiology, University of Nigeria, Nsukka.
2. Department of Zoology, University of Nigeria, Nsukka.
3. Department of Biochemistry, University of Nigeria, Nsukka.
4. Department of Physics and Astronomy
Corresponding author: Nnamonu, Emmanuel Ikechukwu (nnamonuei@yahoo.com, +2348064855635)
Abstract
Insecticides resistance and the corresponding health and environmental challenges that arises as a result of the
use of synthetic chemical based insecticides prompts the search for better alternative control measures which are
more effective, specific in action and less toxic. The 4th instar larvae of Anopheles spp, the vector of the deadly
plasmodium were evaluated in this research against aqueous and methanolic leaf extracts of the plant Picralima
nitida. Results revealed that aqueous leaf extract of the test plant had a mean mortality of 11 at 24hrs exposure
and concentration of 5.0mg/ml. 95% mortality was also recorded at 5.0mg/ml after 48hrs exposure. Methanolic
leaf extract had a mean mortality of 7.7 at 48hrs exposure time and same concentration of 5.0mg/ml. however, at
72hrs exposure, (concentration 5.0mg/ml), the mean mortality increased to 19.3 (97% mortality). The Median
Lethal Time evaluated using probit analysis at 95% Confidence Limit showed the average lethal time of the test
organism Anopheles larvae to the methanolic extract to be 55hrs and 29hrs for the aqueous leaf extract. This
result hence supports the fact that leaf extracts of P. nitida can be used as a source of eco-friendly alternatives in
the control of mosquito vectors, if developed.
Keywords: Resistance, Insecticides, Anopheles spp, Eco-friendly, Larviciding
1. Introduction
Mosquitoes are vectors of disease causing agents found within almost all tropical and subtropical countries. They
are responsible for the transmission of pathogens causing some of the most life-threatening and debilitating
diseases of man, like malaria, yellow fever, dengue fever, filariasis, encephalitis, etc (Chandra et al., 2008 and
ICMR Bulletin, 2003).Which has put 55% of the world’s population at risk in 124 countries (Beatty et al., 2007).
According to WHO/UNICEF, 2005 in their first comprehensive report of the Roll Back Malaria partnership,
malaria is endemic in 117 countries with some 3.2 billion people living in risk areas all over the world.
Anopheline mosquitoes are the vector responsible for the transmission of malaria (Wendy et al., 2012). Around
the world, the medical and economic burden caused by vector-borne diseases continues to grow as current
control measures fail to cope (Radhika et al., 2011). There is therefore an urgent need to identify new control
strategies that will remain effective, even in the face of growing insecticide and drug resistance (Achs and
Malaney, 2002). Repetitive use of man-made insecticides for mosquito control disrupts natural biological control
systems and lead to reemergence of mosquito populations (Radhika et al., 2011). It also resulted in the
development of resistance, detrimental effects on non-target organisms and human health problems and
subsequently this initiated a search for alternative control measures (Das et al., 2007 Zhang et al., 2011). The use
of biological control agents such as predatory fish (Legner, 1995), bacteria (Becker & Ascher, 1998), protozoa
(Chapman, 1974), fungus (Murugesan, 2009), nematodes (Kaya & Gaugler, 1993) and plant products (Mathur,
2003) had shown promising results in the control of mosquito populations. The development of new strategies,
including naturally occurring larvicides in the control of mosquitoes, is important in order to counter the
evolution of resistance in target populations and the possible effects on non-target organisms (Cetin and
Yanikoglu, 2006). The work An Investigation of the Lethality of Picralima nitida, Family Apocynaceae In
Malaria Vector Control was therefore carried out to explore the possibility of using this plant against these
deadly insect vectors. The plant Picralima nitida, family Apocynaceae has for many years been used by rural
dwellers in Oguta and other parts of southeastern Nigeria for the treatment of various diseases like malaria and
other forms of fever. The use of this plant against pathogenic diseases was examined by (Nwakile et al., 2011
and Okokon et al., 2007) examined its use against protozoan infections (Dibua et al., 2013 and Ubulom et al.,
2012) examined the larvicidal effect of the leaf samples while (Ubulom et al., 2012) also evaluated the
antifungal effects.
77
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2. Materials and Methods
2.1. Collection of Plant samples
Fruits and leaves of the Plant Picralima nitida were collected from Oguta Local Government Area of Imo State,
Nigeria. The plant was identified by a professional plant traxonomist at the herbarium section of the International
Center for Ethno–Medicine and Drug Development, Nsukka, Enugu State, Nigeria.
2.2. Photochemical Screening
Screening was conducted in accordance with (Evans and Treas, 2002). The phytochemical tested for includes;
saponin, steroid, tannins, terpenes, alkaloids, glycosides and flavonoids.
2.3. Preparation of Samples
Leaf and seed samples were collected washed and air dried under shady condition at room temperature
(28±2OC). 1g of each dried samples was then pulverized and extracted using a soxhlet extractor. Extracts were
then evapourized using a rotary evaporator until solvents were completely evaporated to get the solidified crude
extracts. The crude extracts thus obtained was stored in sterilized amber coloured bottles and maintained at 4oc
in a refrigerator.
2.4. Stock Preparation
2.5g of the extracts was solubilized in 5ml dimethylsulphuroxide (DMSO), the mixture was then made up with
495ml of water to get a stock solution of 5mg/ml. Graded concentrations of 4mg/ml, 3mg/ml, 2mg/ml, 1mg/ml
and 0.5mg/ml.
2.5. Larvae Identification
Larvae used for this research were identified by a professional parasitologist from the Department of Zoology,
University of Nigeria, Nsukka. They lacked the air tube (Siphon) which is possessed by other genus of mosquito.
They lay parallel to the water surface unlike other genus that stays at an angle to the water surface.
2.6. Procedures for Insect Rearing:
About 100 unparasitalized 4th instar larvae of the insect were collected from flooded grass field. The larvae were
identified by a parasitologist as Anopheles by a parasitologist at the department of Zoology University of
Nigeria, Nsukka. Larvae collected were reared in a bowl protected with net to trap the adults when they emerge.
The larvae were fed with quaker oat. Emerged adult mosquitoes were transferred to an oviposition cage with
potted plants. Adult mosquitoes were fed with 10% glucose solution, laboratory reared albino rats were also
provided as source of blood meal for the adults. Ovitraps were placed within the cage for laying of eggs. Eggs
laid were collected on daily basis and were recycled to the 4th instar before they were used for the bioassay.
2.7. Bioassay
Larvicidal bioassay of individual plant extracts was tested against 4th instar larvae of Anopheles spp. The tests
were conducted, in accordance with (WHO, 2005) protocol with slight modification. Three replicates and a
control were run simultaneously during each trial. Twenty healthy larvae were introduced into each glass beaker
and mortality was observed at 24, 48 and 72 hrs. Larvicidal activity of each extract was determined, by counting
the number of dead larvae on daily basis (24hrs interval). The dead larvae in the three replicates were combined
and expressed as percentage mortality for each concentration. Larvae were recorded dead when they failed to
move after probing with a needle.
When the control mortality ranged from five to twenty per cent, the observed percentage mortality was corrected
by Abbott’s (Abbott, 1925) formula.
78
% Po – % Pc
P = X 100
100 – % Pc
Where P is the corrected mortality, Po is the observed mortality and Pc is the control
Mortality, all expressed in percentages.
2.8. Statistical Analysis
Results obtained from research were analysed using probit analysis as developed by [24]. Statistical package foe
social sciences (SPSS) version 16 was used for the analysis.
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Vol.23, 2014
3. Results
Phytochemical screening of the plant revealed the presence of bioactive ingredients in the leaf extract of the test
plant. Test showed that P. nitida is rich in alkaloids, flavonoids, saponins, tannins, terpenes and glycosides.
3.1. Bioassay
The bioassay results as shown in table 1 and 2 shows the mean mortality values of the two extracts. Their
percentage mortality at the various time of exposure is also shown in the tables. Result shows that the aqueous
leaf extract showed a mean mortality of 11 after 24 hours exposure at 5.0mg/ml, but at 48 hours exposure the
mean mortality increased to 19 which indicates 95% of the test larvae. Mean mortality for the methanolic leaf
extract after 24 hours exposure at 5.0mg/ml was 3, but increased to 19.3 after 72 hours exposure at the same
concentration. This amounts to a percentage mortality of 97%.
3.2. Lethal Median Time
The lethal median time of extracts at the various concentrations, expressed as log of time is shown in Figure 1.
The average lethal time for which approximately 50 percent of larvae exposed would die was 55 hours for
methanolic extract were as that of the aqueous leaf extract is approximately 29 hours.
4. Discussion
Larviciding as a strategy in the fight against insect pest is gaining grounds as most research focuses on the
elimination of pest at their immature stage rather than their adult forms. The use of plants and plant products has
also been a focus research area in the search for possible alternative to chemical based insecticides. Shaalan
(2005) , reviewed the application of larvicide from botanical origin as an essential part of IMM, and various
mosquito control agents such as ocimenone, rotenone, capllin, quassin, thymol, eugenol, neolignans, arborine
and goniothalamin. The larvicidal effect of the plant P. nitida has been previously studied by (Ubulom et al.,
2012 Dibua et al., 2013). The use of plants belonging to the family “Apocynaceae” was also reported to exhibit
significant cidal effect on the larvae of various insect pest by (Al-Doghairi et al., 2004). Phytochemical
screening of the plant revealed the presence of phytochemicals corresponding to those reported by previous
works. The mortality was more with the aqueous leaf extract, showing a mean percentage mortality of 95% at
48hrs exposure at a concentration of 5.0mg/ml and a mean percentage mortality of 100% after 72hrs exposure at
4.0mg/ml (Table 1), while the methanolic extracts showed 38% mortality at 48hrs exposure and concentration of
5.0mg/ml, and 95% mortality at 72hrs exposure with concentration of 3.0mg/ml (Table 2). This confirms the
findings of (Dibua et al., 2013) who gave LC50 and LC95 values of 3.141mg/ml and 42.154mg/ml, 0.352mg/ml
and 4.730mg/ml and 0.164mg/ml and 2.201mg/ml for aqueous leaf extracts at 24, 48 and 72h. LC50 values for
methanolic leaf extract were 48.383mg/ml, 15.817mg/ml and 0.333mg/ml at 24h, 48h and 72 h. This hence
suggests that P. nitida has a higher proportion of methanol soluble bio- active components than water soluble
components. The phytochemical compounds detected in the leaf of P. nitida have been reported to have
biological activity (Lee, 2000 and Wiesman, 2006). Evaluation of the Median lethal time of the test larvae to the
various extracts revealed the average LT50 values to be 55 hours for methanolic extract and 29 hours for aqueous
extract (Figure 1). The results of this work therefore suggest that P. nitida is rich in bioactive components which
are active against the insect vector Anopheles spp. It further calls for more work on the specific effect of the
individual phytochemicals present in this plant. Again the effect of environmental factors like turbidity of water,
temperature and pH should also be investigated as these factors has been reported by (Paaijmans, 2008) to have
an effect on the survival rate of larva.
5. Conclusion
P. nitida is rich in bioactive components which are active against the insect vector Anopheles spp.This result
hence supports the fact that leaf extracts of P. nitida can be used as a source of eco-friendly alternatives in the
control of mosquito vectors, if developed.
Acknowledgements
We sincerely appreciate our research collaborators for their keen interest and support, and also Mr. and Mrs.
M.U. Nwabor for their financial support. Finally, we declare no conflict of interest among the authors.
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Table 1: Larvicidal Effect of Aqueous Leaf Extracts
81
Conc.
(mg/ml)
Mean
±
SD
24hrs
Mean
% Mortality
Mean
±
SD
48hrs
Mean
% Mortality
Mean
±
SD
72hrs
Mean
% Mortality
0.0 0.0 ± 0.0 0 0.0 ± 0.0 0 0.0 ± 0.0 0
0.5 2.3 ± 0.6 12 10.0 ± 1.7 50 12.0 ± 1.0 60
1.0 7.7 ± 1.2 38 17.0 ± 1.0 85 19.3 ± 1.2 97
2.0 8.0 ± 2.6 40 17.0 ± 1.7 85 19.7 ± 0.6 98
3.0 9.3 ± 1.5 47 18.3 ± 0.6 92 19.7 ± 0.6 98
4.0 9.7 ± 3.2 48 18.7 ± 1.5 93 20.0 ± 0.0 100
5.0 11.0 ± 1.0 55 19.0 ± 1.0 95 20.0 ± 0.0 100
Table 2: Larvicidal Effect of Methanolic Leaf Extracts
Conc.
(mg/ml)
Mean
±
SD
24hrs
Mean
% Mortality
Mean
±
SD
48hrs
Mean
% Mortality
Mean
±
SD
72hrs
Mean
% Mortality
0.0 0.0 ± 0.0 0 0.0 ± 0.0 0 0.0 ± 0.0 0
0.5 0.0 ± 0.0 0 2.7 ± 0.6 13 8.3 ± 1.5 42
1.0 0.0 ± 0.0 0 4.3 ± 1.2 22 16.7 ± 1.5 83
2.0 0.0 ± 0.0 0 5.0 ± 1.0 25 17.7 ± 1.5 88
3.0 0.3 ± 0.6 2 5.7 ± 1.5 28 19.0 ± 1.0 95
4.0 1.0 ± 1.0 5 7.0 ± 2.0 35 19.3 ± 0.6 97
5.0 3.0 ± 1.0 15 7.7 ± 0.6 38 19.3 ± 0.6 97
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Vol.23, 2014
Figure 1: 50 Percent Lethal Concentration for Extracts
82
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