This document provides a summary of Andrew Heuze's education and career experience. He received a BSc in Biological Sciences in 2001 and has over 20 years of experience in life sciences research, primarily in assay development and high-throughput screening. His career includes roles at AstraZeneca from 1996-2012 developing cell-based and biochemical assays and running primary screens of over 1 million compounds. He currently works at Thermo Fisher Scientific running biochemical screens for pharmaceutical companies.

1. Andrew Joseph Heuzé BSc (Hons)
Address : 50D High Street Telephone: 0141 814 5913 (home)
Renfrew 07940504111 (mobile)
Renfrewshire, email: andyheuze@gmail.com
PA4 8QP
EDUCATION.
2001: Manchester Metropolitan University: BSc (Hons) Biological Sciences (immunology, physiology,
biochemistry & molecular biology)
1993: Stockport College of Further Education: HNC Electronic (analogue) engineering
1985: Coney Green comprehensive school, Radcliffe, Manchester: O-levels in Maths, English,
Physics, Chemistry, Computer studies, Geography
Skills
Flow Cytometry (and Fluorescent microscopy), Biochemical and cell based assay
development, ELISA, HTRF, fluorescent/luminescent/ assays), High throughput screening, IT &
Computing skills, Electronic engineering, Automation/robotics support, high speed/ nanolitre
liquid dispensers,1536MTP assay design/optimisation , customer support, Mathematics,
Statistics
CAREER DETAILS.
2014 –present day : Thermo Fisher Scientific (ex Life Technologies)
Member of a small team running biochemical screens (FRET/Binding) for global pharma in
an ISO 9001:2008 accredited laboratory. Due to the small number of personnel involved in
all aspects of the laboratory: production/screening & planning, data analysis, training &
documentation, stock management, receiving customer compounds and subsequent
management and usage on the ECHO550.
Achievements: in a very high paced laboratory, rapidly became competent in screening
methodologies and production planning. Brought back into usage 96-channel pipette
(Nanoscreen Multimek™) – learning how to program & set-up, training and ISO
documentation
1996 - 2012: AstraZeneca plc. - Research Bioscientist
My main role was technical and scientific support for the secondary screening and HTS operations for
oncology and inflammation projects ensuring delivery of high quality reliable screening data for cell
based and biochemical small molecule compound screens. I ran screens utilising many assay
technologies whilst running the departmental flow cytometry laboratory. Experienced with many assay
formats – ELISA, Biochemical (fluorescent/luminescent/colourimetric), radiochemical, high content
screening with specific support for Flow Cytometry, Luminex, FMAT8200, FLIPR, mammalian cell
culture and laboratory automation. I was also a member of a core team of scientists generating data for
core screening activities and preparation/solubilisation of compounds using automated liquid handling
systems (TecanEVO, Tecan Genesis, Hamilton and BioMek robots as well as PlateMate Plus &
Agiilent systems).
Main Responsibilities included (but not limited to)
 Optimisation of in-vitro biological assays for automated (robotic) liquid handling systems
o Including development of novel programming solutions
o Selection of appropriate technology/ liquid handling solutions to deliver the HTS
effectively
 Cell culture and provision of cells to selected cell-based assays
o Performed day to day maintenance of mammalian cell lines
 Performing HTS primary screens [on >1 million compounds]
o including compound & reagent supply and/or procurement

2.  close liaison with compound management to ensure correct supply and format of
assay ready plates
o data analysis and reporting of data
o writing scientific reports to support conclusions
 supported secondary screening activities
o cell based (FLIPR/FMAT/Flow Cytometry) and biochemical (FRET) assays
o supported automated liquid handling equipment as a member of a core screening
facility
 ‘Super-User’ for selected automated systems and ancillary equipment
o Including Biocell/Agilent robotic systems, BioRaptr dispensers
o Bespoke plate-based washer/dispenser automated assay systems
 Expert user for Flow Cytometry & FLIPR
 Provided internal and external service for cell sorting using Becton Dickinson cell
sorters ; Operated FACSCan, FACSCalibur, LSR2 and FACSVantage
cytometers
o Support for experimental design and also presentation and interpretation of data
o Maintained an awareness of the scientific literature and commercial press
o Experienced in many aspects of flow Cytometry – immunophenotyping, multiplexing,
DNA (ploidy) analysis, rare event counting & cell sorting
 Ran radiochemical membrane binding assays for the departmental HERG screening
 Experienced user of ArrayScan technology
 Departmental risk and COSHH assessor & Local Laboratory safety coordinator
Main achievements
Nominated person for several departmental technologies.
 Introduced systems to ensure correct operation of small volume dispensers : Instigated a
regular regime of cleaning and calibration that otherwise would have been ad-hoc
 For 10years managed flow Cytometry laboratory maintaining 3 flow cytometers for use of the
research department and providing support for both internal and external customers
o Introduced networking capabilities for flow Cytometry, plate based (HTS) sampling and
new software
o Trained scientists to use new systems and perform data analysis
o Negotiated exchange of old equipment to extend capabilities –e.g. new red lasers.
o Presented successful case to management for expansion of facilities
 i.e HTS processing, replacement of FACSCan with FACSCalibur and BD LSR
o assisted flow cytometer operators in an external affiliated laboratory to set-up ad operate
their flow cytometers
o Assisted in the preparation for the Becton Dickinson user meeting at Zeneca
 Primary user of the FLIPR
o Increased effectiveness and efficiency of FLIPR usage through optimisation and
standardisation of assays with consultation with fellow scientists
o In consultation with other users updated FLIPR 384MTP format after presenting
extensive test results
o completed intensive FLIPR screen with positive recognition (gift) from higher
management
 Brought in impedance based technology (CellKey) for departmental testing
 Collaborated inter-departmentally to test new SPR systems (SRUBIND)
o Collaborated with SRU to optimise non-adherent cell assays
o Asked to present at SBS/ELRIG
 Extended support to Luminx100 (BioPlex) and FMAT8200 systems when introduced
Performed risk assessments for all laboratory equipment making the department compliant with new
interpretation of HSE regulations utilising AstraZeneca cross departmental templates
1993 to 1996 – Assistant Research Bioscientist - ZENECA
Compound screening for Bioscience projects, performing ex-vivo (isolated tissue) safety pharmacology

3. Responsibilities included:
 Cell adhesion (colourimetric) and ELISA type assays for integrin project.
 Dissection of biological tissue, setting up and running the isolated tissue assays – mouse vas
deferens; guinea pig ileum, atria (right and left) and trachea; rat aorta and fundus.
 flow cytometry support
Achievements:
Learned several new techniques in rapid succession and interpretation of pharmocological data
Brought in new technology to update outmoded technology – replaced with digital recording system.
Quickly learned to operate flow cytometers and interpret complex multi-parametric biological data
1986 to 1993 – Assistant Research Bioscientist – ICI
I held a primarily technical role in the Bio-Electronics Unit. That was a dedicated small
group providing novel solutions for monitoring, recording and processing biological data,
primarily for the cardiovascular laboratories.
Responsibilities included:
 Calibration, repair, maintenance and training for externally sourced and in-house produced
laboratory electronic equipment.
 Design and manufacturing novel bespoke small electronic modules (signal processing units) and
ancillary items (e.g. miniature electrodes, interfaces) for use in cardiovascular laboratories.
 Using CAD software, PCB design and manufacture
 On-demand and in-situ laboratory equipment fault-finding & repair.
 Support for the in-house designed physiological telemetry system and acoustic Doppler flow
sensors
Achievements included:
 Design and Implementation of an instantaneous Heart Rate monitor.
 Improved design of Heat Rate calibrator by incorporating digital integrated circuits.
 Award of HNC Applied Biology and HNC (analogue) electronic engineering
1985 YTS placement at ICI
Introduction to Ex-vivo safety screening
Completed ONC Applied Biology with distinction (Biology was not my main choice at school)
1984 Apprentice printer at Bibby and Baron Ltd.
Additional training
Training in Microsoft Outlook, Word, Excel and PowerPoint. Knowledge of Java programming (and a
little SQL) Work based & university training in statistical analysis including use of SpotFire, Prism and
Origin software packages for analysing and presenting data,
Safety training for laboratory safety coordinator and departmental Risk/ COSHH assessor and radio-
isotope usage
Other interests
General interests include football and astronomy; I possessed a 14inch Dobsonian telescope
until I moved house and played regular football with colleagues and non-work related
friends.
I have a continued interest in IT (repaired several PCs) and automation technologies, and
electronics in general.
Publications
Jones SW. Brockbank SMV. Mobbs ML. Le Good NJ. Soma-Haddrick S. Heuze AJ. Langham CJ.
Timms D. Newham P. Needham MRC. The orphan G-protein coupled receptor RDC1: evidence
for a role in chondrocyte hypertrophy and articular cartilage matrix turnover. [Article]
Osteoarthritis & Cartilage. 14(6):597-608, 2006 Jun
Monks NR. Blakey DC. Curtin NJ. East SJ. Heuze A. Newell DR. Induction of apoptosis by the ADEPT agent
ZD2767: comparison with the classical nitrogen mustard chlorambucil and a monofunctional ZD2767
analogue. [Article] British Journal of Cancer. 85(5):764-771, 2001 Sep 1