NADIR: the European “Network for
Animal Diseases Infectiology
Research” facilities

European
Commission

Lantier Frederic ...
Objectives of Research Institutes
in Veterinary Science
To prevent and control infectious diseases of livestock (& wild fa...
NADIR: a platform for level 3 (BSL3)
animal facilities
 The rational of level 3 (BSL3) containment in animal facilities i...
NADIR: the main European centres
for research on
livestock diseases and zoonoses
 In Europe, a number of laboratories wit...
NADIR
EU INFRASTRUCTURE NETWORK

VESO

UEDIN
MRI

AU
DTU

IAH

FLI

CVI

AH-VLA
UNOT

INRA
ANSES
PTP
INIA

CRESA

KVI

5
An integrating infrastructure project
A 2009-2013 project dedicated to:
 Promote exchange of experience between animal fa...
Networking activities
 Exchange of animal lines and DNA concerned chicken, sheep, pig and
fishes:
– Genomic and functiona...
NADIR research activities
RA are designed to sustain the improvement of the services provided by the
infrastructures along...
NADIR transnational access
Access work packages: 14 facilities
Call for access is organized through NA3.2 "Outreach to new...
TA proposals Accepted per country (total 45)

3
1
2
1

2
1

8
1
1
1
1
1

3
1
3
1
1
1
1

7
1

1

1
1

4
1
1
1

6
1
1
1
Species used in Transnational access demands 
and fields of interest

SPECIES

  nbrs. of 
TAs

PIG
FISH
SHEEP
CHICKEN
CAT...
Proven usefulness of the 
NADIR concept
 Improved knowledge of each other and of available resources: an
efficient way to...
 Overall Strategy for the future
RA- Research

NA- Networking
Coordinating:
 Biosecurity
 Biosafety
 Resources
 Exchan...
Examples of links with «One health» strategies
Research objective: finding the adapted equilibrium …
Actions

Expected  ef...
 Thank you for your attention !
VESO

UEDIN
MRI

AU
DTU

IAH

FLI

CVI

AH-VLA
UNOT
ANSES

INRA
PTP

INIA

CRESA

KVI

15
Monitoring tools

Whole genome micro-arrays for transcriptome analysis in :
• Cattle:
– a 90K feature array custom from Co...
Institutes performing TA’s
INSTITUTES
INRA (FR)
CRESA (ES)
CVI (NL)
VESO (NOR)
DTU, LINDHOLM (DK)
DTU, AARHUS (DK)
PTP (IT...
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NADIR: the European “Network for Animal Diseases Infectiology Research” facilities

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GRF 2nd One Health Summit 2013: Presentation by Frederic Lantier, NADIR

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NADIR: the European “Network for Animal Diseases Infectiology Research” facilities

  1. 1. NADIR: the European “Network for Animal Diseases Infectiology Research” facilities European Commission Lantier Frederic 1, Stockhofe, Norbert2, Charleston Bryan3, Blanco Esther4, Simmons Hugh5, Williams John6, Wisselink Henk2, Balkema-Buschmann Anne7, Groschup Martin7, NADIR Partners8 1 ISP, INRA, Nouzilly, France. 2CVI, Wageningen University, Lelystad, The Netherlands. 3 IAH, Institute of Animal Health, Pirbright, United Kingdom. 4CISA-INIA, Madrid, Spain. 5 AH-VLA, Weybridge, United Kingdom. 6PTP, Parco Technologico Padano, Lodi, Italy. 7 FLI, Friedrich Loeffler institute, Isle of Riems, Germany. 8 NADIR Partners, http://www.nadir-project.eu/ or see the Poster FP7 - EU programme (2009-2013) Capacities – infrastructure The overall objective of the Research Infrastructures part of this specific programme is to optimise the use and development of the best research infrastructures existing in Europe. Infrastructure for animal diseases, including zoonoses
  2. 2. Objectives of Research Institutes in Veterinary Science To prevent and control infectious diseases of livestock (& wild fauna) and their transmission to human: 70% of human diseases originate from animals – – – – – – Cattle Sheep and Goat Pigs Poultry Fishes (Wild reservoirs…)  Requests reproducing the disease in target species and animal models, making possible the evaluation and improvement of new diagnostic, prevention and therapeutic tools: • Respecting biosafety, biosecurity and ethical regulations > Using adapted confined animal facilities (BSL3) 2
  3. 3. NADIR: a platform for level 3 (BSL3) animal facilities  The rational of level 3 (BSL3) containment in animal facilities is to Secure animal experimental infections for studies of highly transmissible virulent pathogens, in order to:  Respond to scientific questions: – Pathophysiology of highly transmissible infectious diseases – Conditions of pathogens transmission (analytic epidemiology) – Host Immune response and disease resistance  Validate prophylactic strategies: – Diagnostic tools – Determine infectious tissues (food chain) and contaminating animals – Vaccinations, Treatments and Selection of Resistant genotypes  Make tissues from infected animals (and healthy controls) available: – Material for laboratory investigations – Reference samples for the validation of diagnostic techniques – Pathogen strains collections 3
  4. 4. NADIR: the main European centres for research on livestock diseases and zoonoses  In Europe, a number of laboratories with the level 3 of biosafety are dedicated to the study of infectious diseases.  Among experimental research units, only a few have consequent experimental capacities and are open to the whole scientific community.  They lean on internationally known laboratories, offering both research tools for the analysis of biological samples (BSL3 laboratories) and hosting capacities for scientists.  They decided to take the opportunity of the infrastructure EC projects to coordinate their action to improve interactions between:  Animal infectiology research laboratories  High containment animal facilities with similar or complementary capacities  Academic and private users (including new users and new countries). 4
  5. 5. NADIR EU INFRASTRUCTURE NETWORK VESO UEDIN MRI AU DTU IAH FLI CVI AH-VLA UNOT INRA ANSES PTP INIA CRESA KVI 5
  6. 6. An integrating infrastructure project A 2009-2013 project dedicated to:  Promote exchange of experience between animal facilities (NA)  Facilitate collaborative research to improve existing animal facilities and technologies (RA)  Provide access “free of charge” to external users (TA), including all the infrastructural, logistical, technological and scientific support (adding training courses, travel and subsistence for users): access costs are defined on the basis of “user fees” related to the costs of the infrastructure (calculated as “monthly" costs; e.g; 10 accesses/ 20 K€) EC is providing 7.5 partner: M€ for 17 partners, i.e. in average 500 K€ per – 20% (100 K€) for Networking activities – 40% (200 K€) for Research activities – 40% (200 K€) for Transnational access 6
  7. 7. Networking activities  Exchange of animal lines and DNA concerned chicken, sheep, pig and fishes: – Genomic and functional characterization of these lines  Exchange of procedures and best practices has been initiated through : – The Biosafety/biosecurity meetings : reach a consensual report advising animal facilities for the containment of farm animal species infected with BSL3 pathogens – Listing of validated animal models – The ethical aspects’ meetings (UNOTT 2011, 2012). – Telemetry and imaging techniques: exchange of experience in telemetric transmission to monitor the animal response.  Exchange of technique and laboratory resources: – Cell lines listed, exchanged and tested – High throughput molecular tools (hosts and pathogens) – Collections of infected samples Communications on the NADIR TA in various meetings, publications and through the NADIR Newsletter, website and blog 7
  8. 8. NADIR research activities RA are designed to sustain the improvement of the services provided by the infrastructures along three axes: RA1: Better characterization of animal lines (P7 INIA, Esther Blanco) RA 1.1 - Chicken lines RA 1.2 - Histocompatible and SPF outbred pig lines RA 1.3 - Sheep lines RA 1.4 - Transgenic mice RA 1.5 - Fishes (Trout and Salmon) RA 1.6 - Immunological tools, specific for livestock species. RA2: Development of infection monitoring tools (P13 CVI, Henk Wisselink) RA 2.1 - Upgrading imaging technologies RA 2.2 - Telemetry RA 2.3 - New molecular tools for monitoring infection processes RA 2.4 - Multi-diagnostic tools RA3: Development of new infection animal models (P5 FLI, Anne Buschmann) RA 3.1 - Cell culture RA 3.2 - Vector borne pathogens: AHSV, RVFV, ASFV, West Nile virus, BTV … RA 3.3 - Emerging aquatic animal diseases 8
  9. 9. NADIR transnational access Access work packages: 14 facilities Call for access is organized through NA3.2 "Outreach to new users" and the NADIR Executive committee has: – Nominated the independent Expert Panel. – Organised “the permanent NADIR call for access” (web) – Put in place the procedures for selection (every 2 months) – Evaluates the outcomes of the permanent call through reports and questionaires The call outlines: – The resources (animal facilities) and services available at each partner site – The support offered at each partner site – The conditions of access: member of EU state, publication of project results The selection of the candidates has to take into account: - The nature and quality of their research project - Their motivation for accessing the NADIR facilities – Their potential for disseminating their results – Their EU member state and accessing facilities outside their country – Mainly external demands (external to the Nadir partners) 9
  10. 10. TA proposals Accepted per country (total 45) 3 1 2 1 2 1 8 1 1 1 1 1 3 1 3 1 1 1 1 7 1 1 1 1 4 1 1 1 6 1 1 1
  11. 11. Species used in Transnational access demands  and fields of interest SPECIES   nbrs. of  TAs PIG FISH SHEEP CHICKEN CATTLE BADGER MOUSE 17 11 6 5 4 1 1 45 OBJECTIVE VIRAL PATHOGENESIS TSE PAHOGENESIS BACT.  PATHOGENESIS VIRAL VACCINES IMMUNOLOGY GENETICS BACTERIAL VACCINES PARASITES EPIDEMIOLOGY HOST SUSCEPTIBILITY  nbrs. of TAs 12 3 2 11 7 3 2 1 2 2
  12. 12. Proven usefulness of the  NADIR concept  Improved knowledge of each other and of available resources: an efficient way to shorten delays and to improve response efficiency in case of emergence (e.g. Schmallenberg)  Improved capacities of NADIR infrastructure: molecular and immunological tools, telemetry , imaging, models of infection …for about 40 publications presently acknowledging NADIR  Complementary research studies have been performed, prioritising a limited number of infectious diseases to work on, while taking into account recent evolution of the European sanitary status: Blue-Tongue, Schmallenberg, Tuberculosis, West Nile fever  45 transnational accesses have been provided: most of them would have NOT been done without the NADIR-European support: infrastructure, money and skills availability  Collaboration increased, competition decreased: reducing the barriers to 12 exchange results and difficulties … and requesting help
  13. 13.  Overall Strategy for the future RA- Research NA- Networking Coordinating:  Biosecurity  Biosafety  Resources  Exchanges  Ethics  Accesses   Sustainability  Third countries Phenotyping Animal models In vivo / in vitro  Services  (BSL3 facilities) Access to facilities and  to samples (TA) Monitoring tools Imaging and telemetric technologies Improved Response to Emerging Diseases High throughput molecular technologies Bioinformatics 13
  14. 14. Examples of links with «One health» strategies Research objective: finding the adapted equilibrium … Actions Expected  effect Treatments Treatments Stamping out Negative impact Test alternatives  Stamping out • Cure • Creating resistances • Limit losses • Environment • Decrease contamination pathogen Environment contamination excretion • Better molecules or reduce doses • Prevention: adapted vaccines, host resistance, environment. • Limit epidemic • Limit human contamination • Eradicate • Treatments • Vaccines • Other preventive measures • Social distress • Loss of selection • Loss of exploitations 14
  15. 15.  Thank you for your attention ! VESO UEDIN MRI AU DTU IAH FLI CVI AH-VLA UNOT ANSES INRA PTP INIA CRESA KVI 15
  16. 16. Monitoring tools Whole genome micro-arrays for transcriptome analysis in : • Cattle: – a 90K feature array custom from CombiMatrix which has been tested in studies of mastitis, Johne’s Disease and BASE. – In addition an array of 600 miRNA probes is available • Chickens: – A set of 31K probes representing the unique chicken transcripts has been designed. Can be re-used (x 4 times) with no loss of quality. Expression micro-arrays have been designed for pathogens: • Mycobacterium paratuberculosis • Influenza virus Pathogens (Salmonella, Eimeria) labelled for fluorescence imaging Devices (bolus or sc) developped for telemetric transmission of host 16 infection parameters in cattle, sheep and pigs
  17. 17. Institutes performing TA’s INSTITUTES INRA (FR) CRESA (ES) CVI (NL) VESO (NOR) DTU, LINDHOLM (DK) DTU, AARHUS (DK) PTP (IT) INIA (ES) MOREDUN (SCO) AHVLA (GB) PIRBRIGHT (GB) ANSES (FR) FLI (GER) ACCEPTED PROPOSALS 7 5 5 5 4 4 4 3 3 2 2 1 1

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